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The map label for this gene is fruA

Identifier: 16272394

GI number: 16272394

Start: 468265

End: 469935

Strand: Reverse

Name: fruA

Synonym: HI0446

Alternate gene names: 16272394

Gene position: 469935-468265 (Counterclockwise)

Preceding gene: 16272395

Following gene: 16272387

Centisome position: 25.68

GC content: 41.59

Gene sequence:

>1671_bases
ATGAAGTTATTTTTAACCCAATCAGCTAATGTAGGCGATGTGAAAGCCTATTTATTACATGAAGTTTTTCGAGCAGCTGC
ACAAAAAGCAAATGTCTCTATTGTAGGAACACCAGCCGAAGCAGATCTTGTTCTCGTATTTGGTTCTGTATTACCAAATA
ACCCCGATTTAGTTGGTAAAAAAGTCTTTATTATCGGCGAAGCTATCGCCATGATTTCTCCTGAAGTCACGCTGGCAAAT
GCTTTAGCCAATGGGGCTGATTATGTTGCACCGAAAAGTGCGGTGAGTTTTACAGGTGTTTCTGGTGTAAAAAATATTGT
TGCGGTAACTGCTTGTCCAACTGGCGTTGCACATACGTTTATGTCGGCTGAAGCGATTGAAGCCTATGCGAAAAAGCAAG
GTTGGAATGTAAAAGTTGAAACCCGTGGTCAAGTTGGGGCTGGCAATGAAATTACTGTGGAAGAAGTCGCAGCGGCTGAT
TTGGTATTTGTTGCAGCAGATATTGACGTGCCTTTAGATAAATTTAAAGGTAAACCAATGTACCGCACTTCAACAGGCTT
AGCCTTAAAGAAAACTGAGCAAGAATTTGATAAAGCATTTAAAGAAGCCAAAATTTTTGATGGTGGAAATAATGCGGGAA
CGAAAGAAGAAAGCCGTGAGAAAAAAGGTGTGTACAAACACTTAATGACTGGTGTTTCTCATATGTTACCGTTAGTGGTT
GCTGGTGGATTGTTGATCGCGATTTCCTTTATGTTCAGTTTTAATGTAATTGAAAATACAGGAGTTTTTCAAGATCTTCC
AAATATGTTGATAAATATCGGTAGTGGTGTGGCGTTCAAGTTAATGATTGCCGTGTTTGCTGGCTACGTTGCATTTTCTA
TCGCTGATCGTCCAGGACTTGCAGTTGGTTTGATTGCAGGGATGCTGGCAAGTGAAGCGGGTGCAGGGATTCTTGGCGGT
ATTATTGCTGGTTTCTTAGCGGGCTATGTGGTGAAAGGGCTAAATGTGATTATTCGATTGCCAGCGAGTCTGACTTCATT
AAAACCGATTTTAATTTTACCGCTCTTAGGTTCGATGATTGTTGGTTTGACGATGATTTACCTCATCAATCCACCAGTGG
CTGAAATTATGAAAGAGCTAAGTAATTGGCTTACTTCAATGGGCGAAGTGAATGCGATTGTGTTAGGCGCAATTATCGGC
GCAATGATGTGTATTGATATGGGCGGGCCAGTAAATAAAGCAGCGTACACTTTTTCGGTTGGATTGATTGCTTCCCAAGT
TTATACACCAATGGCTGCGGCAATGGCCGCAGGTATGGTTCCTCCAATTGGTATGACTGTTGCGACTTGGATTGCTCGCA
ATAAATTTACCGTTAGCCAATGCGATGCGGGGAAAGCATCATTTGTTCTTGGTTTATGTTTTATTTCTGAAGGAGCATTA
CCATTTGTGGCAGCAGATCCAATTCGAGTCATTATTAGCTCTGTGATTGGTGGTGCGGTGGCTGGTGCGATTTCAATGGG
ATTAAATATTACCTTACAAGCTCCGCACGGCGGATTATTTGTCATTCCATTTGTGTCTGAGCCGTTGAAATATCTTGGTG
CAATTGCGATTGGTGCTTTATCAACAGGTGTGGTTTACGCCATTATTAAATCGAAAAACAATGCGGAATAA

Upstream 100 bases:

>100_bases
TCCGCTTTTGCTGTATCTCAGAGTAACGTCGGTGTGAGTGATTTGAGTTTACTTGACCCTATTTTAGAAAAAGTACAAAT
AACGATGATTGAAGGATAGT

Downstream 100 bases:

>100_bases
TACCGCACTTTGAATGTAAAAAATAATTTTCATTGAAAAAGTTAGACTATGAAATCAAGGACTAGGCTCTGTAATCTACA
GGGCTTAGTCCTTTTAGTTA

Product: PTS system fructose-specific IIBC component

Products: NA

Alternate protein names: EIIBC-Fru; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MKLFLTQSANVGDVKAYLLHEVFRAAAQKANVSIVGTPAEADLVLVFGSVLPNNPDLVGKKVFIIGEAIAMISPEVTLAN
ALANGADYVAPKSAVSFTGVSGVKNIVAVTACPTGVAHTFMSAEAIEAYAKKQGWNVKVETRGQVGAGNEITVEEVAAAD
LVFVAADIDVPLDKFKGKPMYRTSTGLALKKTEQEFDKAFKEAKIFDGGNNAGTKEESREKKGVYKHLMTGVSHMLPLVV
AGGLLIAISFMFSFNVIENTGVFQDLPNMLINIGSGVAFKLMIAVFAGYVAFSIADRPGLAVGLIAGMLASEAGAGILGG
IIAGFLAGYVVKGLNVIIRLPASLTSLKPILILPLLGSMIVGLTMIYLINPPVAEIMKELSNWLTSMGEVNAIVLGAIIG
AMMCIDMGGPVNKAAYTFSVGLIASQVYTPMAAAMAAGMVPPIGMTVATWIARNKFTVSQCDAGKASFVLGLCFISEGAL
PFVAADPIRVIISSVIGGAVAGAISMGLNITLQAPHGGLFVIPFVSEPLKYLGAIAIGALSTGVVYAIIKSKNNAE

Sequences:

>Translated_556_residues
MKLFLTQSANVGDVKAYLLHEVFRAAAQKANVSIVGTPAEADLVLVFGSVLPNNPDLVGKKVFIIGEAIAMISPEVTLAN
ALANGADYVAPKSAVSFTGVSGVKNIVAVTACPTGVAHTFMSAEAIEAYAKKQGWNVKVETRGQVGAGNEITVEEVAAAD
LVFVAADIDVPLDKFKGKPMYRTSTGLALKKTEQEFDKAFKEAKIFDGGNNAGTKEESREKKGVYKHLMTGVSHMLPLVV
AGGLLIAISFMFSFNVIENTGVFQDLPNMLINIGSGVAFKLMIAVFAGYVAFSIADRPGLAVGLIAGMLASEAGAGILGG
IIAGFLAGYVVKGLNVIIRLPASLTSLKPILILPLLGSMIVGLTMIYLINPPVAEIMKELSNWLTSMGEVNAIVLGAIIG
AMMCIDMGGPVNKAAYTFSVGLIASQVYTPMAAAMAAGMVPPIGMTVATWIARNKFTVSQCDAGKASFVLGLCFISEGAL
PFVAADPIRVIISSVIGGAVAGAISMGLNITLQAPHGGLFVIPFVSEPLKYLGAIAIGALSTGVVYAIIKSKNNAE
>Mature_556_residues
MKLFLTQSANVGDVKAYLLHEVFRAAAQKANVSIVGTPAEADLVLVFGSVLPNNPDLVGKKVFIIGEAIAMISPEVTLAN
ALANGADYVAPKSAVSFTGVSGVKNIVAVTACPTGVAHTFMSAEAIEAYAKKQGWNVKVETRGQVGAGNEITVEEVAAAD
LVFVAADIDVPLDKFKGKPMYRTSTGLALKKTEQEFDKAFKEAKIFDGGNNAGTKEESREKKGVYKHLMTGVSHMLPLVV
AGGLLIAISFMFSFNVIENTGVFQDLPNMLINIGSGVAFKLMIAVFAGYVAFSIADRPGLAVGLIAGMLASEAGAGILGG
IIAGFLAGYVVKGLNVIIRLPASLTSLKPILILPLLGSMIVGLTMIYLINPPVAEIMKELSNWLTSMGEVNAIVLGAIIG
AMMCIDMGGPVNKAAYTFSVGLIASQVYTPMAAAMAAGMVPPIGMTVATWIARNKFTVSQCDAGKASFVLGLCFISEGAL
PFVAADPIRVIISSVIGGAVAGAISMGLNITLQAPHGGLFVIPFVSEPLKYLGAIAIGALSTGVVYAIIKSKNNAE

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain

Homologues:

Organism=Escherichia coli, GI1788492, Length=556, Percent_Identity=56.6546762589928, Blast_Score=593, Evalue=1e-171,
Organism=Escherichia coli, GI1786951, Length=432, Percent_Identity=34.7222222222222, Blast_Score=241, Evalue=1e-64,
Organism=Escherichia coli, GI87082348, Length=428, Percent_Identity=31.0747663551402, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI1790386, Length=319, Percent_Identity=39.4984326018809, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1788729, Length=387, Percent_Identity=28.1653746770026, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1790387, Length=90, Percent_Identity=50, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI1790390, Length=65, Percent_Identity=41.5384615384615, Blast_Score=63, Evalue=5e-11,
Organism=Escherichia coli, GI1788730, Length=89, Percent_Identity=35.9550561797753, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTFBC_HAEIN (P44714)

Other databases:

- EMBL:   L42023
- PIR:   I64068
- RefSeq:   NP_438607.1
- ProteinModelPortal:   P44714
- SMR:   P44714
- GeneID:   949670
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0446
- NMPDR:   fig|71421.1.peg.420
- TIGR:   HI_0446
- HOGENOM:   HBG704192
- OMA:   ARRKFAQ
- ProtClustDB:   CLSK892369
- BioCyc:   HINF71421:HI_0446-MONOMER
- BRENDA:   2.7.1.69
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR003353
- InterPro:   IPR006327
- TIGRFAMs:   TIGR00829
- TIGRFAMs:   TIGR01427

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB

EC number: =2.7.1.69

Molecular weight: Translated: 57707; Mature: 57707

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: PS51099 PTS_EIIB_TYPE_2; PS51104 PTS_EIIC_TYPE_2

Important sites: ACT_SITE 112-112

Signals:

None

Transmembrane regions:

HASH(0xcbdc03c)-; HASH(0xbf5e2e4)-; HASH(0xc86c37c)-; HASH(0xcbe7808)-; HASH(0xccd2a08)-; HASH(0xbea722c)-; HASH(0xbfda4a0)-; HASH(0xc1a8a7c)-; HASH(0xbde5f48)-; HASH(0xae88e68)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFLTQSANVGDVKAYLLHEVFRAAAQKANVSIVGTPAEADLVLVFGSVLPNNPDLVGK
CEEEEECCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEEECCCCCCCCCCCC
KVFIIGEAIAMISPEVTLANALANGADYVAPKSAVSFTGVSGVKNIVAVTACPTGVAHTF
EEEEEEHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEECCHHHHHHEEEEEECCCHHHHHH
MSAEAIEAYAKKQGWNVKVETRGQVGAGNEITVEEVAAADLVFVAADIDVPLDKFKGKPM
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHCEEEEEECCCCCHHHHCCCCC
YRTSTGLALKKTEQEFDKAFKEAKIFDGGNNAGTKEESREKKGVYKHLMTGVSHMLPLVV
EECCCCCEEHHHHHHHHHHHHHCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AGGLLIAISFMFSFNVIENTGVFQDLPNMLINIGSGVAFKLMIAVFAGYVAFSIADRPGL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEECCCCCH
AVGLIAGMLASEAGAGILGGIIAGFLAGYVVKGLNVIIRLPASLTSLKPILILPLLGSMI
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHCCHHHHHHHHHHHH
VGLTMIYLINPPVAEIMKELSNWLTSMGEVNAIVLGAIIGAMMCIDMGGPVNKAAYTFSV
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEH
GLIASQVYTPMAAAMAAGMVPPIGMTVATWIARNKFTVSQCDAGKASFVLGLCFISEGAL
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCCC
PFVAADPIRVIISSVIGGAVAGAISMGLNITLQAPHGGLFVIPFVSEPLKYLGAIAIGAL
CEEECCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEECCHHHHHHHHHHHHHHHH
STGVVYAIIKSKNNAE
HHCEEEEEEECCCCCH
>Mature Secondary Structure
MKLFLTQSANVGDVKAYLLHEVFRAAAQKANVSIVGTPAEADLVLVFGSVLPNNPDLVGK
CEEEEECCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEEECCCCCCCCCCCC
KVFIIGEAIAMISPEVTLANALANGADYVAPKSAVSFTGVSGVKNIVAVTACPTGVAHTF
EEEEEEHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEECCHHHHHHEEEEEECCCHHHHHH
MSAEAIEAYAKKQGWNVKVETRGQVGAGNEITVEEVAAADLVFVAADIDVPLDKFKGKPM
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHCEEEEEECCCCCHHHHCCCCC
YRTSTGLALKKTEQEFDKAFKEAKIFDGGNNAGTKEESREKKGVYKHLMTGVSHMLPLVV
EECCCCCEEHHHHHHHHHHHHHCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AGGLLIAISFMFSFNVIENTGVFQDLPNMLINIGSGVAFKLMIAVFAGYVAFSIADRPGL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEECCCCCH
AVGLIAGMLASEAGAGILGGIIAGFLAGYVVKGLNVIIRLPASLTSLKPILILPLLGSMI
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHCCHHHHHHHHHHHH
VGLTMIYLINPPVAEIMKELSNWLTSMGEVNAIVLGAIIGAMMCIDMGGPVNKAAYTFSV
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEH
GLIASQVYTPMAAAMAAGMVPPIGMTVATWIARNKFTVSQCDAGKASFVLGLCFISEGAL
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCCC
PFVAADPIRVIISSVIGGAVAGAISMGLNITLQAPHGGLFVIPFVSEPLKYLGAIAIGAL
CEEECCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEECCHHHHHHHHHHHHHHHH
STGVVYAIIKSKNNAE
HHCEEEEEEECCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800