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The map label for this gene is aspS [H]

Identifier: 16272271

GI number: 16272271

Start: 347763

End: 349529

Strand: Reverse

Name: aspS [H]

Synonym: HI0317

Alternate gene names: 16272271

Gene position: 349529-347763 (Counterclockwise)

Preceding gene: 161350014

Following gene: 30995367

Centisome position: 19.1

GC content: 41.88

Gene sequence:

>1767_bases
ATGATGCGTACACATTATTGCGGAGCATTAAACCGTAACAATATCGGACAAGACGTAACATTAAGCGGTTGGGTTCATCG
CCGTCGTGATTTAGGTGGCTTGATTTTTATTGATATGCGCGATCGTGATGGTATTGTGCAAGTTTGTTTTGATCCTAAAT
ATCAAGATGCATTGACAGCGGCTGCTGGGTTACGTAATGAATTCTGTATTCAAATTAAAGGCGAGGTGATCGCTCGTCCT
GATAATCAAATTAATAAAAATATGGCAACAGGCGAAGTGGAAGTGTTAGCGAAAGAATTACGCATCTACAATGCTTCTGA
CGTTTTACCATTAGACTTCAACCAAAATAACACCGAAGAACAACGTTTAAAATACCGTTATTTAGATTTACGTCGTCCTG
AAATGGCGCAGCGTTTGAAAACCCGTGCAAAAATCACCAGCTTTGTGCGTCGCTTTATGGATGATAATGGTTTTCTTGAT
ATTGAAACCCCAATGCTAACCAAAGCAACGCCAGAAGGCGCGCGCGACTATTTAGTGCCAAGCCGTGTGCATAAAGGCAA
ATTCTATGCATTGCCGCAATCACCACAGCTTTTCAAACAGCTTTTAATGATGTCTGGTTTTGACCGCTATTATCAAATCG
TAAAATGTTTCCGTGATGAAGATTTACGAGCAGATCGTCAGCCTGAGTTTACTCAAATCGATGTGGAAACTTCTTTCCTA
ACTGCGCCAGAAGTCCGCGAAATTATGGAACGCATGGTGCACGGCTTATGGCTTGATACCATTGGTGTGGACTTAGGTAA
ATTCCCAGTGATGACTTGGCAAGAAGCAATGCGTCGTTTTGGTTCTGATAAGCCAGATTTGCGTAATCCATTAGAAATGG
TAGATGTAGCAGATATTGTCAAAGATGTTGAATTTAAAGTATTTAATGAGCCAGCAAACAATCCAAATGGCCGTGTTGCA
GTCATTCGAGTACCAAATGGTGCAGAAATTACTCGTAAACAAATTGATGAATATACACAATTTGTAGGCATCTACGGTGC
GAAAGGTTTGGCTTGGGCAAAAGTAAACGATATTAATGCTGGTCTTGAAGGCGTGCAAAGTCCGATTGCGAAATTCTTAA
ATGAAGATGTATGGAAAGGGTTAGCAGAACGTGTGAATGCACAAACTGGCGATATTTTATTCTTTGGTGCAGACAAATGG
CAAACTACCACTGATGCAATGGGTGCGTTACGTTTGAAATTAGGTCGTGATCTTGGCTTAACTCGTTTAGACGAATGGCA
ACCGCTTTGGGTCATTGATTTCCCAATGTTTGAACGTGATGAAGAAGGTAATCTTGCAGCAATGCACCATCCATTCACTT
CACCAAAAGATTTCAGCCCAGAGCAATTAGAGGCTGATCCAACAAGTGCGGTAGCCAATGCTTACGATATGGTCATCAAC
GGCTACGAAGTGGGTGGTGGTTCTGTGCGTATTTTTGATCCGAAAATGCAACAAACTGTGTTCCGTATTCTTGGTATTGA
CGAAGAACAACAACGAGAGAAATTCGGTTTCTTATTAGATGCGTTAAAATTTGGTACTCCACCACATGCTGGTTTAGCAT
TCGGTTTAGACCGCTTAACCATGCTTTTAACAGGCACGGAAAACATTCGTGATGTGATTGCCTTTCCGAAAACCACGGCA
GCAGCGTGCTTAATGACGGAAGCCCCGAGTTTTGCAAATCCGCAAGCATTGGAAGAGTTGGCAATTTCTGTCGTTAAAGC
AGAATAA

Upstream 100 bases:

>100_bases
TTGATTTGTCAGCATAAATAACCTTTTTATCTCTCTTGGTTTTCCGCTATAATTGCGACAGATTTTTGAGCATAATTAAA
AGAGTACAAAGGATTTTGAA

Downstream 100 bases:

>100_bases
GTTTGAAATATTAGGAATGAAGTGCGGTCAGATTTGACCGCATTTTTAATGATGCAATACAAAAATAATCAATCTGTTCT
CGTTGTAATTTACACTAAAG

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 588; Mature: 588

Protein sequence:

>588_residues
MMRTHYCGALNRNNIGQDVTLSGWVHRRRDLGGLIFIDMRDRDGIVQVCFDPKYQDALTAAAGLRNEFCIQIKGEVIARP
DNQINKNMATGEVEVLAKELRIYNASDVLPLDFNQNNTEEQRLKYRYLDLRRPEMAQRLKTRAKITSFVRRFMDDNGFLD
IETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFL
TAPEVREIMERMVHGLWLDTIGVDLGKFPVMTWQEAMRRFGSDKPDLRNPLEMVDVADIVKDVEFKVFNEPANNPNGRVA
VIRVPNGAEITRKQIDEYTQFVGIYGAKGLAWAKVNDINAGLEGVQSPIAKFLNEDVWKGLAERVNAQTGDILFFGADKW
QTTTDAMGALRLKLGRDLGLTRLDEWQPLWVIDFPMFERDEEGNLAAMHHPFTSPKDFSPEQLEADPTSAVANAYDMVIN
GYEVGGGSVRIFDPKMQQTVFRILGIDEEQQREKFGFLLDALKFGTPPHAGLAFGLDRLTMLLTGTENIRDVIAFPKTTA
AACLMTEAPSFANPQALEELAISVVKAE

Sequences:

>Translated_588_residues
MMRTHYCGALNRNNIGQDVTLSGWVHRRRDLGGLIFIDMRDRDGIVQVCFDPKYQDALTAAAGLRNEFCIQIKGEVIARP
DNQINKNMATGEVEVLAKELRIYNASDVLPLDFNQNNTEEQRLKYRYLDLRRPEMAQRLKTRAKITSFVRRFMDDNGFLD
IETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFL
TAPEVREIMERMVHGLWLDTIGVDLGKFPVMTWQEAMRRFGSDKPDLRNPLEMVDVADIVKDVEFKVFNEPANNPNGRVA
VIRVPNGAEITRKQIDEYTQFVGIYGAKGLAWAKVNDINAGLEGVQSPIAKFLNEDVWKGLAERVNAQTGDILFFGADKW
QTTTDAMGALRLKLGRDLGLTRLDEWQPLWVIDFPMFERDEEGNLAAMHHPFTSPKDFSPEQLEADPTSAVANAYDMVIN
GYEVGGGSVRIFDPKMQQTVFRILGIDEEQQREKFGFLLDALKFGTPPHAGLAFGLDRLTMLLTGTENIRDVIAFPKTTA
AACLMTEAPSFANPQALEELAISVVKAE
>Mature_588_residues
MMRTHYCGALNRNNIGQDVTLSGWVHRRRDLGGLIFIDMRDRDGIVQVCFDPKYQDALTAAAGLRNEFCIQIKGEVIARP
DNQINKNMATGEVEVLAKELRIYNASDVLPLDFNQNNTEEQRLKYRYLDLRRPEMAQRLKTRAKITSFVRRFMDDNGFLD
IETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFL
TAPEVREIMERMVHGLWLDTIGVDLGKFPVMTWQEAMRRFGSDKPDLRNPLEMVDVADIVKDVEFKVFNEPANNPNGRVA
VIRVPNGAEITRKQIDEYTQFVGIYGAKGLAWAKVNDINAGLEGVQSPIAKFLNEDVWKGLAERVNAQTGDILFFGADKW
QTTTDAMGALRLKLGRDLGLTRLDEWQPLWVIDFPMFERDEEGNLAAMHHPFTSPKDFSPEQLEADPTSAVANAYDMVIN
GYEVGGGSVRIFDPKMQQTVFRILGIDEEQQREKFGFLLDALKFGTPPHAGLAFGLDRLTMLLTGTENIRDVIAFPKTTA
AACLMTEAPSFANPQALEELAISVVKAE

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=604, Percent_Identity=37.7483443708609, Blast_Score=403, Evalue=1e-112,
Organism=Homo sapiens, GI5031815, Length=253, Percent_Identity=29.2490118577075, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI194272210, Length=253, Percent_Identity=29.2490118577075, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI45439306, Length=253, Percent_Identity=24.901185770751, Blast_Score=73, Evalue=9e-13,
Organism=Homo sapiens, GI226491502, Length=271, Percent_Identity=23.2472324723247, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1788173, Length=585, Percent_Identity=76.2393162393162, Blast_Score=927, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=290, Percent_Identity=28.2758620689655, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1790571, Length=289, Percent_Identity=27.3356401384083, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI32566633, Length=600, Percent_Identity=34.1666666666667, Blast_Score=319, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI71994340, Length=248, Percent_Identity=24.1935483870968, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17535925, Length=263, Percent_Identity=22.4334600760456, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17535927, Length=263, Percent_Identity=22.4334600760456, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17551876, Length=298, Percent_Identity=27.1812080536913, Blast_Score=85, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6325153, Length=628, Percent_Identity=34.7133757961783, Blast_Score=295, Evalue=2e-80,
Organism=Saccharomyces cerevisiae, GI6320242, Length=258, Percent_Identity=24.4186046511628, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321807, Length=250, Percent_Identity=25.6, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323011, Length=289, Percent_Identity=25.9515570934256, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24584738, Length=610, Percent_Identity=37.2131147540984, Blast_Score=346, Evalue=3e-95,
Organism=Drosophila melanogaster, GI24640851, Length=331, Percent_Identity=27.7945619335347, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24640849, Length=263, Percent_Identity=29.6577946768061, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI17136276, Length=241, Percent_Identity=26.1410788381743, Blast_Score=75, Evalue=9e-14,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 66640; Mature: 66640

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRTHYCGALNRNNIGQDVTLSGWVHRRRDLGGLIFIDMRDRDGIVQVCFDPKYQDALTA
CCCCCCCCCCCCCCCCCCCEEHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCHHHHHHH
AAGLRNEFCIQIKGEVIARPDNQINKNMATGEVEVLAKELRIYNASDVLPLDFNQNNTEE
HHCCCCCEEEEECCCEEECCCHHHCCCCCCCHHHHHHHHHHEECCCCEEEECCCCCCCHH
QRLKYRYLDLRRPEMAQRLKTRAKITSFVRRFMDDNGFLDIETPMLTKATPEGARDYLVP
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCC
SRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFL
CCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECHHCC
TAPEVREIMERMVHGLWLDTIGVDLGKFPVMTWQEAMRRFGSDKPDLRNPLEMVDVADIV
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
KDVEFKVFNEPANNPNGRVAVIRVPNGAEITRKQIDEYTQFVGIYGAKGLAWAKVNDINA
HHCCEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHH
GLEGVQSPIAKFLNEDVWKGLAERVNAQTGDILFFGADKWQTTTDAMGALRLKLGRDLGL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCC
TRLDEWQPLWVIDFPMFERDEEGNLAAMHHPFTSPKDFSPEQLEADPTSAVANAYDMVIN
CCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCHHHCCCCCHHHHHHHHHHEEE
GYEVGGGSVRIFDPKMQQTVFRILGIDEEQQREKFGFLLDALKFGTPPHAGLAFGLDRLT
EEEECCCEEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
MLLTGTENIRDVIAFPKTTAAACLMTEAPSFANPQALEELAISVVKAE
EEEECCHHHHHHHCCCCHHHHHHHEECCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMRTHYCGALNRNNIGQDVTLSGWVHRRRDLGGLIFIDMRDRDGIVQVCFDPKYQDALTA
CCCCCCCCCCCCCCCCCCCEEHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCHHHHHHH
AAGLRNEFCIQIKGEVIARPDNQINKNMATGEVEVLAKELRIYNASDVLPLDFNQNNTEE
HHCCCCCEEEEECCCEEECCCHHHCCCCCCCHHHHHHHHHHEECCCCEEEECCCCCCCHH
QRLKYRYLDLRRPEMAQRLKTRAKITSFVRRFMDDNGFLDIETPMLTKATPEGARDYLVP
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCC
SRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFL
CCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECHHCC
TAPEVREIMERMVHGLWLDTIGVDLGKFPVMTWQEAMRRFGSDKPDLRNPLEMVDVADIV
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
KDVEFKVFNEPANNPNGRVAVIRVPNGAEITRKQIDEYTQFVGIYGAKGLAWAKVNDINA
HHCCEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHH
GLEGVQSPIAKFLNEDVWKGLAERVNAQTGDILFFGADKWQTTTDAMGALRLKLGRDLGL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCC
TRLDEWQPLWVIDFPMFERDEEGNLAAMHHPFTSPKDFSPEQLEADPTSAVANAYDMVIN
CCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCHHHCCCCCHHHHHHHHHHEEE
GYEVGGGSVRIFDPKMQQTVFRILGIDEEQQREKFGFLLDALKFGTPPHAGLAFGLDRLT
EEEECCCEEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
MLLTGTENIRDVIAFPKTTAAACLMTEAPSFANPQALEELAISVVKAE
EEEECCHHHHHHHCCCCHHHHHHHEECCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA