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The map label for this gene is gltX [H]

Identifier: 16272232

GI number: 16272232

Start: 307435

End: 308877

Strand: Reverse

Name: gltX [H]

Synonym: HI0274

Alternate gene names: 16272232

Gene position: 308877-307435 (Counterclockwise)

Preceding gene: 16272234

Following gene: 16272231

Centisome position: 16.88

GC content: 41.3

Gene sequence:

>1443_bases
ATGAAACTAGATGCTCCTTTTAATTTAGATCCAAATGTAAAAGTGCGTACTCGTTTTGCTCCTAGCCCTACAGGTTATTT
ACATGTAGGTGGCGCACGTACCGCACTTTATTCTTGGTTATACGCAAAACATAATAACGGCGAATTTGTATTACGAATTG
AAGACACTGATTTAGAGCGTTCAACACCTGAAGCCACTGCTGCCATTATTGAAGGCATGGAATGGTTAAATCTTCCGTGG
GAGCATGGCCCTTATTACCAAACTAAACGCTTTGATCGTTATAACCAAGTGATCGATGAAATGATTGAACAAGGCCTAGC
ATATCGTTGTTATTGTACTAAAGAACACTTAGAAGAACTTCGTCATACTCAAGAACAAAATAAAGAAAAACCACGTTATG
ACCGTCACTGCTTACACGATCACAACCATTCACCTGATGAACCCCACGTTGTACGTTTTAAAAATCCAACTGAAGGCTCG
GTGGTATTTGATGATGCCGTACGTGGCCGTATTGAAATCAGCAACAGTGAGCTTGATGATCTTATTATTCGCCGCACAGA
CGGTTCTCCAACTTATAACTTCTGTGTAGTTGTAGATGACTGGGACATGGGTATCACACATGTTGTGCGTGGGGAAGATC
ATATCAACAACACGCCTCGTCAAATTAACATTTTAAAAGCGATTGGTGCCCCAATTCCAACCTATGCACACGTTTCTATG
ATTAATGGCGATGATGGTCAAAAACTCTCTAAACGACATGGCGCAGTAAGCGTAATGCAATATCGTGATGATGGTTACTT
ACCAGAAGCTTTAATTAACTATCTTGTTCGTTTAGGCTGGGGACATGGCGACCAAGAAATTTTTAGTCGCGAAGAAATGA
TCAACTATTTCGAATTAGATCATGTTAGCAAATCAGCTAGCGCATTTAATACCGAAAAATTACAATGGTTGAATCAACAT
TATATCCGTGAATTACCGCCAGAATATGTGGCTAAACACCTTGAATGGCATTACAAAGATCAAGGCATTGATACAAGCAA
TGGCCCTGCATTAACTGAAATAGTCACCATGCTTGCGGAACGTTGCAAAACCTTAAAAGAAATGGCAAGGTCAAGCCGCT
ACTTCTTTGAAGAGTTTGAAACCTTTGATGAAGCCGCAGCGAAAAAACATTTCAAAGGCAATGCTGCTGAAGCGCTTGCA
AAAGTGAAAGAAAAATTAACCGCACTTTCTAGCTGGGATTTACATTCTATTCACGAAGCCATTGAGCAAACAGCTGCTGA
ACTAGAAGTGGGCATGGGTAAAGTTGGCATGCCATTACGTGTCGCGGTGACAGGTTCTGGTCAATCGCCATCAATGGATG
TTACCCTAGTCGGTATCGGCAGAGATCGCGTACTTGCGCGTATTCAGCGTGCTATTGATTTTATTCATGCACAAAACGCT
TAA

Upstream 100 bases:

>100_bases
ATTGTCGTAAAAATAAGCATAAAATCCGCTTTTTTATTTAGAAGTCAAACAAGTCGTAGTAGAATAACGACTAATATTTT
ATTGAAGATAGGTTATAAAA

Downstream 100 bases:

>100_bases
TATTTAATCGGATAACAAACACATAATAAAATCGTATTGACAGCCACATAGTCAAATTTTATCATGTCGCTGTCTTTTAT
GGGGATATAGCTCAGTTGGG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MKLDAPFNLDPNVKVRTRFAPSPTGYLHVGGARTALYSWLYAKHNNGEFVLRIEDTDLERSTPEATAAIIEGMEWLNLPW
EHGPYYQTKRFDRYNQVIDEMIEQGLAYRCYCTKEHLEELRHTQEQNKEKPRYDRHCLHDHNHSPDEPHVVRFKNPTEGS
VVFDDAVRGRIEISNSELDDLIIRRTDGSPTYNFCVVVDDWDMGITHVVRGEDHINNTPRQINILKAIGAPIPTYAHVSM
INGDDGQKLSKRHGAVSVMQYRDDGYLPEALINYLVRLGWGHGDQEIFSREEMINYFELDHVSKSASAFNTEKLQWLNQH
YIRELPPEYVAKHLEWHYKDQGIDTSNGPALTEIVTMLAERCKTLKEMARSSRYFFEEFETFDEAAAKKHFKGNAAEALA
KVKEKLTALSSWDLHSIHEAIEQTAAELEVGMGKVGMPLRVAVTGSGQSPSMDVTLVGIGRDRVLARIQRAIDFIHAQNA

Sequences:

>Translated_480_residues
MKLDAPFNLDPNVKVRTRFAPSPTGYLHVGGARTALYSWLYAKHNNGEFVLRIEDTDLERSTPEATAAIIEGMEWLNLPW
EHGPYYQTKRFDRYNQVIDEMIEQGLAYRCYCTKEHLEELRHTQEQNKEKPRYDRHCLHDHNHSPDEPHVVRFKNPTEGS
VVFDDAVRGRIEISNSELDDLIIRRTDGSPTYNFCVVVDDWDMGITHVVRGEDHINNTPRQINILKAIGAPIPTYAHVSM
INGDDGQKLSKRHGAVSVMQYRDDGYLPEALINYLVRLGWGHGDQEIFSREEMINYFELDHVSKSASAFNTEKLQWLNQH
YIRELPPEYVAKHLEWHYKDQGIDTSNGPALTEIVTMLAERCKTLKEMARSSRYFFEEFETFDEAAAKKHFKGNAAEALA
KVKEKLTALSSWDLHSIHEAIEQTAAELEVGMGKVGMPLRVAVTGSGQSPSMDVTLVGIGRDRVLARIQRAIDFIHAQNA
>Mature_480_residues
MKLDAPFNLDPNVKVRTRFAPSPTGYLHVGGARTALYSWLYAKHNNGEFVLRIEDTDLERSTPEATAAIIEGMEWLNLPW
EHGPYYQTKRFDRYNQVIDEMIEQGLAYRCYCTKEHLEELRHTQEQNKEKPRYDRHCLHDHNHSPDEPHVVRFKNPTEGS
VVFDDAVRGRIEISNSELDDLIIRRTDGSPTYNFCVVVDDWDMGITHVVRGEDHINNTPRQINILKAIGAPIPTYAHVSM
INGDDGQKLSKRHGAVSVMQYRDDGYLPEALINYLVRLGWGHGDQEIFSREEMINYFELDHVSKSASAFNTEKLQWLNQH
YIRELPPEYVAKHLEWHYKDQGIDTSNGPALTEIVTMLAERCKTLKEMARSSRYFFEEFETFDEAAAKKHFKGNAAEALA
KVKEKLTALSSWDLHSIHEAIEQTAAELEVGMGKVGMPLRVAVTGSGQSPSMDVTLVGIGRDRVLARIQRAIDFIHAQNA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=505, Percent_Identity=29.5049504950495, Blast_Score=225, Evalue=8e-59,
Organism=Homo sapiens, GI4826960, Length=297, Percent_Identity=26.2626262626263, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI62241042, Length=338, Percent_Identity=23.6686390532544, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1788743, Length=464, Percent_Identity=72.4137931034483, Blast_Score=723, Evalue=0.0,
Organism=Escherichia coli, GI226510925, Length=243, Percent_Identity=37.037037037037, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1786895, Length=122, Percent_Identity=35.2459016393443, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71993394, Length=478, Percent_Identity=30.9623430962343, Blast_Score=201, Evalue=9e-52,
Organism=Saccharomyces cerevisiae, GI6324540, Length=501, Percent_Identity=30.938123752495, Blast_Score=246, Evalue=6e-66,
Organism=Saccharomyces cerevisiae, GI6324742, Length=210, Percent_Identity=30.4761904761905, Blast_Score=90, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6321192, Length=224, Percent_Identity=29.4642857142857, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24665321, Length=494, Percent_Identity=30.9716599190283, Blast_Score=242, Evalue=4e-64,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 54875; Mature: 54875

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLDAPFNLDPNVKVRTRFAPSPTGYLHVGGARTALYSWLYAKHNNGEFVLRIEDTDLER
CCCCCCCCCCCCCEEEEEECCCCCCEEEECCHHHHHHHHHHEEECCCEEEEEEECCCCCC
STPEATAAIIEGMEWLNLPWEHGPYYQTKRFDRYNQVIDEMIEQGLAYRCYCTKEHLEEL
CCHHHHHHHHHCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHH
RHTQEQNKEKPRYDRHCLHDHNHSPDEPHVVRFKNPTEGSVVFDDAVRGRIEISNSELDD
HHHHHHCCCCCCHHHHHHCCCCCCCCCCCEEEECCCCCCCEEEECCCCCEEEECCCCCCC
LIIRRTDGSPTYNFCVVVDDWDMGITHVVRGEDHINNTPRQINILKAIGAPIPTYAHVSM
EEEEECCCCCCEEEEEEEECCCCCCEEEECCCHHCCCCCHHEEHHHHHCCCCCCCEEEEE
INGDDGQKLSKRHGAVSVMQYRDDGYLPEALINYLVRLGWGHGDQEIFSREEMINYFELD
EECCCCHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
HVSKSASAFNTEKLQWLNQHYIRELPPEYVAKHLEWHYKDQGIDTSNGPALTEIVTMLAE
HHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
RCKTLKEMARSSRYFFEEFETFDEAAAKKHFKGNAAEALAKVKEKLTALSSWDLHSIHEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
IEQTAAELEVGMGKVGMPLRVAVTGSGQSPSMDVTLVGIGRDRVLARIQRAIDFIHAQNA
HHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKLDAPFNLDPNVKVRTRFAPSPTGYLHVGGARTALYSWLYAKHNNGEFVLRIEDTDLER
CCCCCCCCCCCCCEEEEEECCCCCCEEEECCHHHHHHHHHHEEECCCEEEEEEECCCCCC
STPEATAAIIEGMEWLNLPWEHGPYYQTKRFDRYNQVIDEMIEQGLAYRCYCTKEHLEEL
CCHHHHHHHHHCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHH
RHTQEQNKEKPRYDRHCLHDHNHSPDEPHVVRFKNPTEGSVVFDDAVRGRIEISNSELDD
HHHHHHCCCCCCHHHHHHCCCCCCCCCCCEEEECCCCCCCEEEECCCCCEEEECCCCCCC
LIIRRTDGSPTYNFCVVVDDWDMGITHVVRGEDHINNTPRQINILKAIGAPIPTYAHVSM
EEEEECCCCCCEEEEEEEECCCCCCEEEECCCHHCCCCCHHEEHHHHHCCCCCCCEEEEE
INGDDGQKLSKRHGAVSVMQYRDDGYLPEALINYLVRLGWGHGDQEIFSREEMINYFELD
EECCCCHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
HVSKSASAFNTEKLQWLNQHYIRELPPEYVAKHLEWHYKDQGIDTSNGPALTEIVTMLAE
HHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
RCKTLKEMARSSRYFFEEFETFDEAAAKKHFKGNAAEALAKVKEKLTALSSWDLHSIHEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
IEQTAAELEVGMGKVGMPLRVAVTGSGQSPSMDVTLVGIGRDRVLARIQRAIDFIHAQNA
HHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA