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The map label for this gene is rph

Identifier: 16272231

GI number: 16272231

Start: 306418

End: 307134

Strand: Reverse

Name: rph

Synonym: HI0273

Alternate gene names: 16272231

Gene position: 307134-306418 (Counterclockwise)

Preceding gene: 16272232

Following gene: 16272230

Centisome position: 16.78

GC content: 43.93

Gene sequence:

>717_bases
ATGCGTCCAAATAATCGAGAAAATAATCAACCCCGCCAAATTAAAATTACCCGTAACTACACCAAGCATGCTGAAGGTTC
TGTGCTTGTTGAATTTGGGGACACCAAAGTGCTTTGTACTGCAACAGTGGAAGATGCTGTGCCACGTTTCTTAAAAGGAC
AAGGGCAAGGTTGGGTAACGGCTGAATATGGAATGTTGCCACGTTCAACACATAGTCGTATGCAACGTGAGGCGGCTAAA
GGTAAACAAGGCGGACGCACAATGGAAATTCAACGCTTAATTGCCCGCTCTTTGCGCGCCATGGTGGATCTTAAAGCACT
GGGTGAACGTGCGATTACTTTAGATTGTGATGTTATTCAAGCGGACGGCGGCACACGAACTGCATCCATTACTGGTGCTG
CAGTCGCATTATGTGATGCGATCAATGGTTTAATTGAGAATGGCACGTTAAAAACTAACCCCATTAAAGGGCTAGTTTCG
GCAATTTCTGTAGGGATTGTTGATGGTCAAGCTGTTTGTGATTTGGAATATGTGGAAGATTCTGCAGCGGAAACCGATAT
GAATGTTGTGATGATGGAAGATGGTCGCATGATTGAGGTGCAAGGCACTGCAGAAGGCGAGCCATTTAGCCATGAAGAAT
TATTAACATTATTAGATTTAGCGAAACAAGGCTGTAATCAAATTTTTATCGCTCAACGCGAAGCGTTAGGCTTATAA

Upstream 100 bases:

>100_bases
AATCAATATCTTAGCGAATTTTAACAAAAATAAAAACTAAAGCGTGCTAGAATAGTGCGCTTTTTTATTACATCGTTCGA
AACCATATCAAGGAATAATT

Downstream 100 bases:

>100_bases
ACACAGTTTATAGGAGTTAAAAAATGGAACAATATAAACGCGATTTTATCGAATTTGCTTTAAGCCGAAATGTATTGAAA
TTCGGCGAGTTTACTTTGAA

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MRPNNRENNQPRQIKITRNYTKHAEGSVLVEFGDTKVLCTATVEDAVPRFLKGQGQGWVTAEYGMLPRSTHSRMQREAAK
GKQGGRTMEIQRLIARSLRAMVDLKALGERAITLDCDVIQADGGTRTASITGAAVALCDAINGLIENGTLKTNPIKGLVS
AISVGIVDGQAVCDLEYVEDSAAETDMNVVMMEDGRMIEVQGTAEGEPFSHEELLTLLDLAKQGCNQIFIAQREALGL

Sequences:

>Translated_238_residues
MRPNNRENNQPRQIKITRNYTKHAEGSVLVEFGDTKVLCTATVEDAVPRFLKGQGQGWVTAEYGMLPRSTHSRMQREAAK
GKQGGRTMEIQRLIARSLRAMVDLKALGERAITLDCDVIQADGGTRTASITGAAVALCDAINGLIENGTLKTNPIKGLVS
AISVGIVDGQAVCDLEYVEDSAAETDMNVVMMEDGRMIEVQGTAEGEPFSHEELLTLLDLAKQGCNQIFIAQREALGL
>Mature_238_residues
MRPNNRENNQPRQIKITRNYTKHAEGSVLVEFGDTKVLCTATVEDAVPRFLKGQGQGWVTAEYGMLPRSTHSRMQREAAK
GKQGGRTMEIQRLIARSLRAMVDLKALGERAITLDCDVIQADGGTRTASITGAAVALCDAINGLIENGTLKTNPIKGLVS
AISVGIVDGQAVCDLEYVEDSAAETDMNVVMMEDGRMIEVQGTAEGEPFSHEELLTLLDLAKQGCNQIFIAQREALGL

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=211, Percent_Identity=82.4644549763033, Blast_Score=368, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI71981632, Length=238, Percent_Identity=25.6302521008403, Blast_Score=74, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_HAEI8 (Q4QNR6)

Other databases:

- EMBL:   CP000057
- RefSeq:   YP_247991.1
- HSSP:   Q9UXC2
- ProteinModelPortal:   Q4QNR6
- SMR:   Q4QNR6
- STRING:   Q4QNR6
- GeneID:   3429705
- GenomeReviews:   CP000057_GR
- KEGG:   hit:NTHI0381
- eggNOG:   COG0689
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- PhylomeDB:   Q4QNR6
- ProtClustDB:   PRK00173
- BioCyc:   HINF281310:NTHI0381-MONOMER
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 25790; Mature: 25790

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPNNRENNQPRQIKITRNYTKHAEGSVLVEFGDTKVLCTATVEDAVPRFLKGQGQGWVT
CCCCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCEEEEEECHHHHHHHHHCCCCCCEEE
AEYGMLPRSTHSRMQREAAKGKQGGRTMEIQRLIARSLRAMVDLKALGERAITLDCDVIQ
EECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEE
ADGGTRTASITGAAVALCDAINGLIENGTLKTNPIKGLVSAISVGIVDGQAVCDLEYVED
CCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEEECCCEEEEHHHHHC
SAAETDMNVVMMEDGRMIEVQGTAEGEPFSHEELLTLLDLAKQGCNQIFIAQREALGL
CCCCCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEHHHHCCC
>Mature Secondary Structure
MRPNNRENNQPRQIKITRNYTKHAEGSVLVEFGDTKVLCTATVEDAVPRFLKGQGQGWVT
CCCCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCEEEEEECHHHHHHHHHCCCCCCEEE
AEYGMLPRSTHSRMQREAAKGKQGGRTMEIQRLIARSLRAMVDLKALGERAITLDCDVIQ
EECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEE
ADGGTRTASITGAAVALCDAINGLIENGTLKTNPIKGLVSAISVGIVDGQAVCDLEYVED
CCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEEECCCEEEEHHHHHC
SAAETDMNVVMMEDGRMIEVQGTAEGEPFSHEELLTLLDLAKQGCNQIFIAQREALGL
CCCCCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA