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The map label for this gene is brnQ

Identifier: 16272189

GI number: 16272189

Start: 255662

End: 256972

Strand: Direct

Name: brnQ

Synonym: HI0226

Alternate gene names: 16272189

Gene position: 255662-256972 (Clockwise)

Preceding gene: 30995364

Following gene: 16272192

Centisome position: 13.97

GC content: 37.76

Gene sequence:

>1311_bases
ATGTTTTCACGCAAAGATATTATCGTTTTAGGAATGATGATCTTCGCCTTATTTTTAGGTGCGGGTAATATCATTTTTCC
GCCTATGGAAGGATTTTCCTCAGGACAACATTGGACGAGTGCTTCCTTAGGCTTTGTTTTAACTGGCGTTTTAATGCCAT
TTATTACGCTTGTGGTGGTAGCGATTTTAGGTCGTGGCGAAGAACTTACTAAAGATTTACCAAAATGGGCTGGCACAGGT
TTCCTCGTTATACTTTATTTAACCATTGGCTCTACCTTTGCAATGCCCCGCATCACTAATGTTGCTTATGAAATGGCGTG
GTTGCCTTTAGGCTTAACAGAAAATAATGCGAATGTTCGTTTTGTATTCTCACTCATTTTTAACTTAATCGCGATGGGCT
TTATGATTAGCCCAAATACGATCATTTCAAGCGTGGGTAAATTTATGACACCAGCTTTATTAGTGTTACTTATTGCTGTA
GCAATTACCGTATTTATTTCGCCTTTATCTGAAATTCAAGCGCCAAGTAATGCTTATGAAAATAGCCATTCTTTATTAAT
CGGTTTAACTAGCGGCTATCAAACGATGGATGTACTTGCAGCCATCGCTTTCGGTGGAATTGTCGCCCGAGCATTATCAG
CGAAAAATGTGACAAAAACCAAAGATATTGTGAAATACACCATTTCAGCAGGATTTGTTTCAGTCATCTTACTTGCGGGT
TTATATTTTTCGCTTTTCTATCTAGGCGCAACTAGTGCTGCAGTTGCAGAAGGGGCAACGAATGGTGGACAAATTTTCTC
TCGTTATGTAAATGTCTTATTCGGTTCAGCTGGGACTTGGATTATGGCAGGTATTATTGTGTTAGCGAGCTTAACAACAC
TTGTTGGTGTAACTAGCGCATCTGCTGATTATTTTTCAAAATTCTCTGTGCGTTTTTCTTATCCATTCTGGGCCGCACTT
TTTACCGCAATGACAATCACTGTTTCACAATATGGTTTAACGGATCTACTTCGCATAACGATCCCAGCCTTGTTATTAAT
TTACCCAGTAGCCATTGTGCTTGTTTTATTGCAATTCTTACGAAAAAAACTTCCATCAATAAAATTTACATACAATAGTA
CATTGCTCGTTACAGTTTGTTTTAGTTTATGTGACAGCTTAAATAACGTTAAAATGTTACCAGAAAGCATCAATTCATTA
TTGAAACACTTCCCTCTTTCATCTGAAGGAATGGCTTGGTTGGTTCCGACTTTAGTAATGTTAGTTGCAAGCATTTTTAT
TGGAAAAGCATTGCATAAAACCCATTCTTAA

Upstream 100 bases:

>100_bases
CCATATATTGTGACAAAATTCACATTTTTAATTTTACACAATAGAGATTTTATCTACTTTGTAGTAGAATGCGGACCTTT
TATTTTTAGAATCAAAAACT

Downstream 100 bases:

>100_bases
TCTCACAAAGTGTTTTATTTATCAGCCCTGTGCCGTAAAACTCCGTCCTTCAGGGCGGAGATATAAGGCACAGGGCTGAT
AAATTTCTAACCGCACTCTT

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MFSRKDIIVLGMMIFALFLGAGNIIFPPMEGFSSGQHWTSASLGFVLTGVLMPFITLVVVAILGRGEELTKDLPKWAGTG
FLVILYLTIGSTFAMPRITNVAYEMAWLPLGLTENNANVRFVFSLIFNLIAMGFMISPNTIISSVGKFMTPALLVLLIAV
AITVFISPLSEIQAPSNAYENSHSLLIGLTSGYQTMDVLAAIAFGGIVARALSAKNVTKTKDIVKYTISAGFVSVILLAG
LYFSLFYLGATSAAVAEGATNGGQIFSRYVNVLFGSAGTWIMAGIIVLASLTTLVGVTSASADYFSKFSVRFSYPFWAAL
FTAMTITVSQYGLTDLLRITIPALLLIYPVAIVLVLLQFLRKKLPSIKFTYNSTLLVTVCFSLCDSLNNVKMLPESINSL
LKHFPLSSEGMAWLVPTLVMLVASIFIGKALHKTHS

Sequences:

>Translated_436_residues
MFSRKDIIVLGMMIFALFLGAGNIIFPPMEGFSSGQHWTSASLGFVLTGVLMPFITLVVVAILGRGEELTKDLPKWAGTG
FLVILYLTIGSTFAMPRITNVAYEMAWLPLGLTENNANVRFVFSLIFNLIAMGFMISPNTIISSVGKFMTPALLVLLIAV
AITVFISPLSEIQAPSNAYENSHSLLIGLTSGYQTMDVLAAIAFGGIVARALSAKNVTKTKDIVKYTISAGFVSVILLAG
LYFSLFYLGATSAAVAEGATNGGQIFSRYVNVLFGSAGTWIMAGIIVLASLTTLVGVTSASADYFSKFSVRFSYPFWAAL
FTAMTITVSQYGLTDLLRITIPALLLIYPVAIVLVLLQFLRKKLPSIKFTYNSTLLVTVCFSLCDSLNNVKMLPESINSL
LKHFPLSSEGMAWLVPTLVMLVASIFIGKALHKTHS
>Mature_436_residues
MFSRKDIIVLGMMIFALFLGAGNIIFPPMEGFSSGQHWTSASLGFVLTGVLMPFITLVVVAILGRGEELTKDLPKWAGTG
FLVILYLTIGSTFAMPRITNVAYEMAWLPLGLTENNANVRFVFSLIFNLIAMGFMISPNTIISSVGKFMTPALLVLLIAV
AITVFISPLSEIQAPSNAYENSHSLLIGLTSGYQTMDVLAAIAFGGIVARALSAKNVTKTKDIVKYTISAGFVSVILLAG
LYFSLFYLGATSAAVAEGATNGGQIFSRYVNVLFGSAGTWIMAGIIVLASLTTLVGVTSASADYFSKFSVRFSYPFWAAL
FTAMTITVSQYGLTDLLRITIPALLLIYPVAIVLVLLQFLRKKLPSIKFTYNSTLLVTVCFSLCDSLNNVKMLPESINSL
LKHFPLSSEGMAWLVPTLVMLVASIFIGKALHKTHS

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family

Homologues:

Organism=Escherichia coli, GI1786601, Length=430, Percent_Identity=34.1860465116279, Blast_Score=256, Evalue=2e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BRNQ_HAEIN (P71345)

Other databases:

- EMBL:   L42023
- PIR:   D64056
- RefSeq:   NP_438398.1
- GeneID:   951144
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0226
- NMPDR:   fig|71421.1.peg.216
- TIGR:   HI_0226
- HOGENOM:   HBG533860
- OMA:   STFAMPR
- ProtClustDB:   CLSK808393
- BioCyc:   HINF71421:HI_0226-MONOMER
- InterPro:   IPR004685
- TIGRFAMs:   TIGR00796

Pfam domain/function: PF05525 Branch_AA_trans

EC number: NA

Molecular weight: Translated: 47040; Mature: 47040

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xaf4cdb4)-; HASH(0xc3cb948)-; HASH(0xb5e4c14)-; HASH(0xc28f8f0)-; HASH(0xc4b4a54)-; HASH(0xc2ad84c)-; HASH(0xb59308c)-; HASH(0xc3b625c)-; HASH(0xc04d5f8)-; HASH(0xc5aa16c)-; HASH(0xafe030c)-;

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSRKDIIVLGMMIFALFLGAGNIIFPPMEGFSSGQHWTSASLGFVLTGVLMPFITLVVV
CCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AILGRGEELTKDLPKWAGTGFLVILYLTIGSTFAMPRITNVAYEMAWLPLGLTENNANVR
HHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCHHCCCHHHHHHHHHHHEECEEECCCCCHH
FVFSLIFNLIAMGFMISPNTIISSVGKFMTPALLVLLIAVAITVFISPLSEIQAPSNAYE
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
NSHSLLIGLTSGYQTMDVLAAIAFGGIVARALSAKNVTKTKDIVKYTISAGFVSVILLAG
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LYFSLFYLGATSAAVAEGATNGGQIFSRYVNVLFGSAGTWIMAGIIVLASLTTLVGVTSA
HHHHHHHHHCHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
SADYFSKFSVRFSYPFWAALFTAMTITVSQYGLTDLLRITIPALLLIYPVAIVLVLLQFL
CHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKKLPSIKFTYNSTLLVTVCFSLCDSLNNVKMLPESINSLLKHFPLSSEGMAWLVPTLVM
HHHCCCEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LVASIFIGKALHKTHS
HHHHHHHHHHHHHCCH
>Mature Secondary Structure
MFSRKDIIVLGMMIFALFLGAGNIIFPPMEGFSSGQHWTSASLGFVLTGVLMPFITLVVV
CCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AILGRGEELTKDLPKWAGTGFLVILYLTIGSTFAMPRITNVAYEMAWLPLGLTENNANVR
HHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCHHCCCHHHHHHHHHHHEECEEECCCCCHH
FVFSLIFNLIAMGFMISPNTIISSVGKFMTPALLVLLIAVAITVFISPLSEIQAPSNAYE
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
NSHSLLIGLTSGYQTMDVLAAIAFGGIVARALSAKNVTKTKDIVKYTISAGFVSVILLAG
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LYFSLFYLGATSAAVAEGATNGGQIFSRYVNVLFGSAGTWIMAGIIVLASLTTLVGVTSA
HHHHHHHHHCHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
SADYFSKFSVRFSYPFWAALFTAMTITVSQYGLTDLLRITIPALLLIYPVAIVLVLLQFL
CHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKKLPSIKFTYNSTLLVTVCFSLCDSLNNVKMLPESINSLLKHFPLSSEGMAWLVPTLVM
HHHCCCEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LVASIFIGKALHKTHS
HHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800