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The map label for this gene is pnp [H]

Identifier: 16272192

GI number: 16272192

Start: 258296

End: 260425

Strand: Direct

Name: pnp [H]

Synonym: HI0229

Alternate gene names: 16272192

Gene position: 258296-260425 (Clockwise)

Preceding gene: 16272189

Following gene: 16272193

Centisome position: 14.11

GC content: 42.46

Gene sequence:

>2130_bases
GTGAATCCAATTGTTAAACAATTTAAATACGGTCAGCATACCGTTACTCTAGAAACTGGTGCGATTGCACGTCAAGCAAC
GGCTGCGGTTATGGCAAGCATGGACGATACCACGGTATTCGTGACTGTTGTTGCTAAAAAAGATGTGAAAGAAGGTCAAG
ACTTCTTCCCATTAACCGTAAACTACCAAGAGCGTACTTATGCGGCGGGTAAAATCCCGGGTGGTTTCTTCAAACGTGAA
GGTCGTCCATCTGAAGGCGAAACTTTAATTGCACGTTTAATTGACCGTCCAATTCGTCCATTATTCCCAGAAGGTTTCTT
CAATGAAATCCAAGTTGTGGCAACTGTGGTTTCTGTAAACCCACAAATCAGCCCAGACTTAGTGGCAATGATCGGTGCTT
CTGCAGCATTAACCTTATCTGGTGTGCCTTTCAACGGCCCTATCGGTGCAGCGCGTGTTGGTTTTATCGACAACCAATTC
GTATTAAACCCAACTATGGCAGAACAAAAACAAAGCCGTTTGGACTTAGTGGTTGCAGGCACTGACAAAGCCGTATTAAT
GGTTGAATCTGAAGCTGATATTTTAACTGAAGAACAAATGTTAGCAGCGGTCGTATTCGGTCATCAACAACAACAAGTTG
TAGTTGAAGCAATCAAAGAATTTGCAAAAGAAGCGGGCAAACCACGTTGGGATTGGGTTGCACCACAACCAAACACAGAT
TTAATCAACAAAGTAAAAGCGATTGCTGAAGCACGTTTAGGCGATGCATACCGCATTACTGAAAAACAATTACGTTACGA
ACAGATCGATGCGATTAAAGCGGATGTAATTGCGCAAATCACAGCTGAAGATGAAGAAAYCAGCGAAGGTAAAATCGTGG
ATATTTTCACCGCACTTGAAAGCCAAATCGTTCGTGGTCGTATCATTGCAGGCGAACCACGTATTGACGGTCGTACCGTT
GATACTGTTCGTGCATTAGATATTTGCACTGGTGTATTACCACGTACTCACGGTTCTGCGATTTTTACTCGTGGCGAAAC
GCAAGCATTAGCTGTTGCAACCTTAGGTACCGAACGTGATGCACAAATCATTGATGAATTAACGGGTGAACGTCAAGATC
ACTTCTTATTCCATTATAATTTCCCTCCATATTCTGTAGGCGAAACAGGTATGATCGGCTCGCCAAAACGTCGTGAGATC
GGTCACGGTCGTTTAGCAAAACGTGGTGTAGCGGCTGTTATGCCAAGCCTTGCAGAATTCCCGTATGTTGTGCGTGTAGT
ATCTGAAATCACTGAATCTAACGGTTCTTCTTCTATGGCGTCTGTATGTGGTGCGTCTTTAGCATTAATGGATGCGGGCG
TTCCAATCAAAGCTGCTGTTGCAGGTATCGCAATGGGCTTAGTGAAAGAAGAAGAAAAATTTGTGGTGCTTTCAGATATT
TTAGGCGATGAAGACCATTTAGGCGATATGGACTTTAAAGTGGCTGGTACACGTGAAGGTGTAACCGCCCTTCAAATGGA
CATCAAAATTGAAGGTATCACCCCTGAAATTATGCAAATTGCATTAAACCAAGCGAAAAGTGCACGTATGCACATTCTTG
GTGTAATGGAACAAGCAATTCCTGCTCCACGTGCTGATATTTCTGACTACGCACCGCGCATCTATACAATGAAAATCGAT
CCTAAGAAAATCAAAGATGTGATTGGTAAAGGTGGTGCAACAATCCGTTCACTAACTGAAGAAACTGGTACATCAATTGA
CATTGATGACGATGGTACAGTGAAAATTGCCGCAGTAGACAGCAATGCAGCGAAAAACGTAATGGGACGTATTGAAGAAA
TCGTTGCAGAAGTTGAAGCTGGCGTAATTTACAAAGGTAAAGTCACTCGTCTTGCTGATTTTGGTGCATTCGTCGCAATC
GTTGGAAATAAAGAAGGTTTAGTTCATATTTCTCAAATTGCTGAAGAGCGTGTAGAAAAAGTTAGCGATTACCTTCAAGT
GGGTCAAGAAGTAAACGTAAAAGTTGTTGAAATCGATCGTCAAGGTCGTATTCGTTTAACAATGAAAGACTTGGCTCCAA
AACAAGAAACTGAAATTAACCAAGAAGATTCTGTCGAAGAACAGGAATAA

Upstream 100 bases:

>100_bases
AAACGCCCAAAGTGCGGTCATTTTTTCGAAAGATTTCTTTTGCTAGTCGCAATCTGGGAAATAAGGCATTGTTTTTAATA
TAAATTTTAAGGAAAACATT

Downstream 100 bases:

>100_bases
TCTTGGAATATAGAATCAAAACTTGGTAGCATTCGCTGCCAAGTTTTGTTGTTTTCCAACTTTTAGGCATAAAGAAAGCA
AATGCGGTGTTTTCGCTTAT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 709; Mature: 709

Protein sequence:

>709_residues
MNPIVKQFKYGQHTVTLETGAIARQATAAVMASMDDTTVFVTVVAKKDVKEGQDFFPLTVNYQERTYAAGKIPGGFFKRE
GRPSEGETLIARLIDRPIRPLFPEGFFNEIQVVATVVSVNPQISPDLVAMIGASAALTLSGVPFNGPIGAARVGFIDNQF
VLNPTMAEQKQSRLDLVVAGTDKAVLMVESEADILTEEQMLAAVVFGHQQQQVVVEAIKEFAKEAGKPRWDWVAPQPNTD
LINKVKAIAEARLGDAYRITEKQLRYEQIDAIKADVIAQITAEDEEXSEGKIVDIFTALESQIVRGRIIAGEPRIDGRTV
DTVRALDICTGVLPRTHGSAIFTRGETQALAVATLGTERDAQIIDELTGERQDHFLFHYNFPPYSVGETGMIGSPKRREI
GHGRLAKRGVAAVMPSLAEFPYVVRVVSEITESNGSSSMASVCGASLALMDAGVPIKAAVAGIAMGLVKEEEKFVVLSDI
LGDEDHLGDMDFKVAGTREGVTALQMDIKIEGITPEIMQIALNQAKSARMHILGVMEQAIPAPRADISDYAPRIYTMKID
PKKIKDVIGKGGATIRSLTEETGTSIDIDDDGTVKIAAVDSNAAKNVMGRIEEIVAEVEAGVIYKGKVTRLADFGAFVAI
VGNKEGLVHISQIAEERVEKVSDYLQVGQEVNVKVVEIDRQGRIRLTMKDLAPKQETEINQEDSVEEQE

Sequences:

>Translated_709_residues
MNPIVKQFKYGQHTVTLETGAIARQATAAVMASMDDTTVFVTVVAKKDVKEGQDFFPLTVNYQERTYAAGKIPGGFFKRE
GRPSEGETLIARLIDRPIRPLFPEGFFNEIQVVATVVSVNPQISPDLVAMIGASAALTLSGVPFNGPIGAARVGFIDNQF
VLNPTMAEQKQSRLDLVVAGTDKAVLMVESEADILTEEQMLAAVVFGHQQQQVVVEAIKEFAKEAGKPRWDWVAPQPNTD
LINKVKAIAEARLGDAYRITEKQLRYEQIDAIKADVIAQITAEDEEXSEGKIVDIFTALESQIVRGRIIAGEPRIDGRTV
DTVRALDICTGVLPRTHGSAIFTRGETQALAVATLGTERDAQIIDELTGERQDHFLFHYNFPPYSVGETGMIGSPKRREI
GHGRLAKRGVAAVMPSLAEFPYVVRVVSEITESNGSSSMASVCGASLALMDAGVPIKAAVAGIAMGLVKEEEKFVVLSDI
LGDEDHLGDMDFKVAGTREGVTALQMDIKIEGITPEIMQIALNQAKSARMHILGVMEQAIPAPRADISDYAPRIYTMKID
PKKIKDVIGKGGATIRSLTEETGTSIDIDDDGTVKIAAVDSNAAKNVMGRIEEIVAEVEAGVIYKGKVTRLADFGAFVAI
VGNKEGLVHISQIAEERVEKVSDYLQVGQEVNVKVVEIDRQGRIRLTMKDLAPKQETEINQEDSVEEQE
>Mature_709_residues
MNPIVKQFKYGQHTVTLETGAIARQATAAVMASMDDTTVFVTVVAKKDVKEGQDFFPLTVNYQERTYAAGKIPGGFFKRE
GRPSEGETLIARLIDRPIRPLFPEGFFNEIQVVATVVSVNPQISPDLVAMIGASAALTLSGVPFNGPIGAARVGFIDNQF
VLNPTMAEQKQSRLDLVVAGTDKAVLMVESEADILTEEQMLAAVVFGHQQQQVVVEAIKEFAKEAGKPRWDWVAPQPNTD
LINKVKAIAEARLGDAYRITEKQLRYEQIDAIKADVIAQITAEDEEXSEGKIVDIFTALESQIVRGRIIAGEPRIDGRTV
DTVRALDICTGVLPRTHGSAIFTRGETQALAVATLGTERDAQIIDELTGERQDHFLFHYNFPPYSVGETGMIGSPKRREI
GHGRLAKRGVAAVMPSLAEFPYVVRVVSEITESNGSSSMASVCGASLALMDAGVPIKAAVAGIAMGLVKEEEKFVVLSDI
LGDEDHLGDMDFKVAGTREGVTALQMDIKIEGITPEIMQIALNQAKSARMHILGVMEQAIPAPRADISDYAPRIYTMKID
PKKIKDVIGKGGATIRSLTEETGTSIDIDDDGTVKIAAVDSNAAKNVMGRIEEIVAEVEAGVIYKGKVTRLADFGAFVAI
VGNKEGLVHISQIAEERVEKVSDYLQVGQEVNVKVVEIDRQGRIRLTMKDLAPKQETEINQEDSVEEQE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=708, Percent_Identity=37.0056497175141, Blast_Score=441, Evalue=1e-123,
Organism=Escherichia coli, GI145693187, Length=691, Percent_Identity=76.1215629522431, Blast_Score=1099, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=706, Percent_Identity=32.1529745042493, Blast_Score=334, Evalue=9e-92,
Organism=Caenorhabditis elegans, GI17535281, Length=93, Percent_Identity=46.2365591397849, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281362905, Length=710, Percent_Identity=37.4647887323944, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24651641, Length=710, Percent_Identity=37.4647887323944, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24651643, Length=710, Percent_Identity=37.4647887323944, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI161079377, Length=656, Percent_Identity=37.1951219512195, Blast_Score=416, Evalue=1e-116,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 76954; Mature: 76954

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPIVKQFKYGQHTVTLETGAIARQATAAVMASMDDTTVFVTVVAKKDVKEGQDFFPLTV
CCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEEECCCHHCCCCEEEEEE
NYQERTYAAGKIPGGFFKREGRPSEGETLIARLIDRPIRPLFPEGFFNEIQVVATVVSVN
ECCCCEEECCCCCCCHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHEEEECC
PQISPDLVAMIGASAALTLSGVPFNGPIGAARVGFIDNQFVLNPTMAEQKQSRLDLVVAG
CCCCHHHHHHHCCCEEEEEECCCCCCCCCCEEEEEECCEEEECCCHHHHHHCCEEEEEEC
TDKAVLMVESEADILTEEQMLAAVVFGHQQQQVVVEAIKEFAKEAGKPRWDWVAPQPNTD
CCCEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCCHH
LINKVKAIAEARLGDAYRITEKQLRYEQIDAIKADVIAQITAEDEEXSEGKIVDIFTALE
HHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEHHHHHH
SQIVRGRIIAGEPRIDGRTVDTVRALDICTGVLPRTHGSAIFTRGETQALAVATLGTERD
HHHHCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEECCCCCH
AQIIDELTGERQDHFLFHYNFPPYSVGETGMIGSPKRREIGHGRLAKRGVAAVMPSLAEF
HHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHH
PYVVRVVSEITESNGSSSMASVCGASLALMDAGVPIKAAVAGIAMGLVKEEEKFVVLSDI
HHHHHHHHHHHHCCCCHHHHHHHHHHHEEHCCCCCHHHHHHHHHHHHHCCCCCEEEEHHH
LGDEDHLGDMDFKVAGTREGVTALQMDIKIEGITPEIMQIALNQAKSARMHILGVMEQAI
CCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHC
PAPRADISDYAPRIYTMKIDPKKIKDVIGKGGATIRSLTEETGTSIDIDDDGTVKIAAVD
CCCCCCHHHCCCEEEEEEECHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCCEEEEEEEC
SNAAKNVMGRIEEIVAEVEAGVIYKGKVTRLADFGAFVAIVGNKEGLVHISQIAEERVEK
CCHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHCCEEEEEEECCCCCEEHHHHHHHHHHH
VSDYLQVGQEVNVKVVEIDRQGRIRLTMKDLAPKQETEINQEDSVEEQE
HHHHHHCCCCCCEEEEEECCCCCEEEEHHHCCCCHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNPIVKQFKYGQHTVTLETGAIARQATAAVMASMDDTTVFVTVVAKKDVKEGQDFFPLTV
CCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEEECCCHHCCCCEEEEEE
NYQERTYAAGKIPGGFFKREGRPSEGETLIARLIDRPIRPLFPEGFFNEIQVVATVVSVN
ECCCCEEECCCCCCCHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHEEEECC
PQISPDLVAMIGASAALTLSGVPFNGPIGAARVGFIDNQFVLNPTMAEQKQSRLDLVVAG
CCCCHHHHHHHCCCEEEEEECCCCCCCCCCEEEEEECCEEEECCCHHHHHHCCEEEEEEC
TDKAVLMVESEADILTEEQMLAAVVFGHQQQQVVVEAIKEFAKEAGKPRWDWVAPQPNTD
CCCEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCCHH
LINKVKAIAEARLGDAYRITEKQLRYEQIDAIKADVIAQITAEDEEXSEGKIVDIFTALE
HHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEHHHHHH
SQIVRGRIIAGEPRIDGRTVDTVRALDICTGVLPRTHGSAIFTRGETQALAVATLGTERD
HHHHCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEECCCCCH
AQIIDELTGERQDHFLFHYNFPPYSVGETGMIGSPKRREIGHGRLAKRGVAAVMPSLAEF
HHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHH
PYVVRVVSEITESNGSSSMASVCGASLALMDAGVPIKAAVAGIAMGLVKEEEKFVVLSDI
HHHHHHHHHHHHCCCCHHHHHHHHHHHEEHCCCCCHHHHHHHHHHHHHCCCCCEEEEHHH
LGDEDHLGDMDFKVAGTREGVTALQMDIKIEGITPEIMQIALNQAKSARMHILGVMEQAI
CCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHC
PAPRADISDYAPRIYTMKIDPKKIKDVIGKGGATIRSLTEETGTSIDIDDDGTVKIAAVD
CCCCCCHHHCCCEEEEEEECHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCCEEEEEEEC
SNAAKNVMGRIEEIVAEVEAGVIYKGKVTRLADFGAFVAIVGNKEGLVHISQIAEERVEK
CCHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHCCEEEEEEECCCCCEEHHHHHHHHHHH
VSDYLQVGQEVNVKVVEIDRQGRIRLTMKDLAPKQETEINQEDSVEEQE
HHHHHHCCCCCCEEEEEECCCCCEEEEHHHCCCCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100; 10873488 [H]