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The map label for this gene is msbB

Identifier: 16272163

GI number: 16272163

Start: 214867

End: 215823

Strand: Direct

Name: msbB

Synonym: HI0199

Alternate gene names: 16272163

Gene position: 214867-215823 (Clockwise)

Preceding gene: 16272162

Following gene: 16273684

Centisome position: 11.74

GC content: 39.92

Gene sequence:

>957_bases
ATGTCGGATAATCAACAAAATTTACGTTTGACGGCGAGAGTGGGCTATGAAGCGCACTTTTCATGGTCGTATTTAAAGCC
TCAATATTGGGGGATTTGGCTTGGTATTTTCTTTTTATTGTTGTTAGCATTTGTGCCTTTTCGTCTGCGCGATAAATTGA
CGGGAAAATTAGGTATTTGGATTGGGCATAAAGCAAAGAAACAGCGTACGCGTGCACAAACTAACTTGCAATATTGTTTC
CCTCATTGGACTGAACAACAACGTGAGCAAGTGATTGATAAAATGTTTGCGGTTGTCGCTCAGGTTATGTTTGGTATTGG
TGAGATTGCCATCCGTTCAAAGAAACATTTGCAAAAACGCAGCGAATTTATCGGTCTTGAACATATCGAACAGGCAAAAG
CTGAAGGAAAGAATATTATTCTTATGGTGCCACATGGCTGGGCGATTGATGCGTCTGGCATTATTTTGCACACTCAAGGC
ATGCCAATGACTTCTATGTATAATCCACACCGTAATCCATTGGTGGATTGGCTTTGGACGATTACACGCCAACGTTTCGG
CGGAAAAATGCATGCACGCCAAAATGGTATTAAACCTTTTTTAAGTCATGTTCGTAAAGGCGAAATGGGTTATTACTTAC
CCGATGAAGATTTTGGGGCGGAACAAAGCGTATTTGTTGATTTCTTTGGGACTTATAAAGCGACATTACCAGGGTTAAAT
AAAATGGCAAAACTTTCTAAAGCCGTTGTTATTCCAATGTTTCCTCGTTATAACGCTGAAACGGGCAAATATGAAATGGA
AATTCATCCTGCAATGAATTTAAGTGATGATCCTGAACAATCAGCCCGAGCAATGAACGAAGAAATAGAATCTTTTGTTA
CGCCAGCGCCAGAGCAATATGTTTGGATTTTGCAATTATTGCGTACAAGGAAAGATGGCGAAGATCTTTATGATTAA

Upstream 100 bases:

>100_bases
TCTTTTATGATGACGGCTAAAATGGTTTACGATCAGGGTTGGTTTCATTTTTAATTCGGAAAGCGCGCAAAAGTGCGGTT
AAAATTAATTACATTTTATT

Downstream 100 bases:

>100_bases
AAATAGGGTTTGAAAGTCATATTCTCGAAAACGGATATTCAATAAGGTAAATAAGAAATGGAAGAAAAAATTCGGCTGAC
GCAATACAGCCATGGTGCAG

Product: lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MSDNQQNLRLTARVGYEAHFSWSYLKPQYWGIWLGIFFLLLLAFVPFRLRDKLTGKLGIWIGHKAKKQRTRAQTNLQYCF
PHWTEQQREQVIDKMFAVVAQVMFGIGEIAIRSKKHLQKRSEFIGLEHIEQAKAEGKNIILMVPHGWAIDASGIILHTQG
MPMTSMYNPHRNPLVDWLWTITRQRFGGKMHARQNGIKPFLSHVRKGEMGYYLPDEDFGAEQSVFVDFFGTYKATLPGLN
KMAKLSKAVVIPMFPRYNAETGKYEMEIHPAMNLSDDPEQSARAMNEEIESFVTPAPEQYVWILQLLRTRKDGEDLYD

Sequences:

>Translated_318_residues
MSDNQQNLRLTARVGYEAHFSWSYLKPQYWGIWLGIFFLLLLAFVPFRLRDKLTGKLGIWIGHKAKKQRTRAQTNLQYCF
PHWTEQQREQVIDKMFAVVAQVMFGIGEIAIRSKKHLQKRSEFIGLEHIEQAKAEGKNIILMVPHGWAIDASGIILHTQG
MPMTSMYNPHRNPLVDWLWTITRQRFGGKMHARQNGIKPFLSHVRKGEMGYYLPDEDFGAEQSVFVDFFGTYKATLPGLN
KMAKLSKAVVIPMFPRYNAETGKYEMEIHPAMNLSDDPEQSARAMNEEIESFVTPAPEQYVWILQLLRTRKDGEDLYD
>Mature_317_residues
SDNQQNLRLTARVGYEAHFSWSYLKPQYWGIWLGIFFLLLLAFVPFRLRDKLTGKLGIWIGHKAKKQRTRAQTNLQYCFP
HWTEQQREQVIDKMFAVVAQVMFGIGEIAIRSKKHLQKRSEFIGLEHIEQAKAEGKNIILMVPHGWAIDASGIILHTQGM
PMTSMYNPHRNPLVDWLWTITRQRFGGKMHARQNGIKPFLSHVRKGEMGYYLPDEDFGAEQSVFVDFFGTYKATLPGLNK
MAKLSKAVVIPMFPRYNAETGKYEMEIHPAMNLSDDPEQSARAMNEEIESFVTPAPEQYVWILQLLRTRKDGEDLYD

Specific function: Transfers myristate or laurate, activated on ACP, to (KDO)2-(lauroyl)-lipid IVA

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family

Homologues:

Organism=Escherichia coli, GI1788161, Length=301, Percent_Identity=45.1827242524917, Blast_Score=299, Evalue=2e-82,
Organism=Escherichia coli, GI1787292, Length=300, Percent_Identity=32, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI87082094, Length=301, Percent_Identity=28.2392026578073, Blast_Score=139, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSBB_HAEIN (P44567)

Other databases:

- EMBL:   L42023
- PIR:   I64053
- RefSeq:   NP_438368.1
- GeneID:   951108
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0199
- NMPDR:   fig|71421.1.peg.189
- TIGR:   HI_0199
- HOGENOM:   HBG730280
- OMA:   FGTYKAT
- ProtClustDB:   PRK08943
- BioCyc:   HINF71421:HI_0199-MONOMER
- InterPro:   IPR004960
- InterPro:   IPR011921
- TIGRFAMs:   TIGR02208

Pfam domain/function: PF03279 Lip_A_acyltrans

EC number: 2.3.1.-

Molecular weight: Translated: 36883; Mature: 36752

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xbbcea20)-;

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNQQNLRLTARVGYEAHFSWSYLKPQYWGIWLGIFFLLLLAFVPFRLRDKLTGKLGIW
CCCCCCCEEEEEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCEEEEE
IGHKAKKQRTRAQTNLQYCFPHWTEQQREQVIDKMFAVVAQVMFGIGEIAIRSKKHLQKR
ECCHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SEFIGLEHIEQAKAEGKNIILMVPHGWAIDASGIILHTQGMPMTSMYNPHRNPLVDWLWT
HHHCCHHHHHHHHHCCCEEEEEECCCCEECCCCEEEEECCCCCHHCCCCCCCHHHHHHHH
ITRQRFGGKMHARQNGIKPFLSHVRKGEMGYYLPDEDFGAEQSVFVDFFGTYKATLPGLN
HHHHHHCCHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHCCCHH
KMAKLSKAVVIPMFPRYNAETGKYEMEIHPAMNLSDDPEQSARAMNEEIESFVTPAPEQY
HHHHHHHEEEEECCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHH
VWILQLLRTRKDGEDLYD
HHHHHHHHHCCCCCCCCH
>Mature Secondary Structure 
SDNQQNLRLTARVGYEAHFSWSYLKPQYWGIWLGIFFLLLLAFVPFRLRDKLTGKLGIW
CCCCCCEEEEEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCEEEEE
IGHKAKKQRTRAQTNLQYCFPHWTEQQREQVIDKMFAVVAQVMFGIGEIAIRSKKHLQKR
ECCHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SEFIGLEHIEQAKAEGKNIILMVPHGWAIDASGIILHTQGMPMTSMYNPHRNPLVDWLWT
HHHCCHHHHHHHHHCCCEEEEEECCCCEECCCCEEEEECCCCCHHCCCCCCCHHHHHHHH
ITRQRFGGKMHARQNGIKPFLSHVRKGEMGYYLPDEDFGAEQSVFVDFFGTYKATLPGLN
HHHHHHCCHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHCCCHH
KMAKLSKAVVIPMFPRYNAETGKYEMEIHPAMNLSDDPEQSARAMNEEIESFVTPAPEQY
HHHHHHHEEEEECCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHH
VWILQLLRTRKDGEDLYD
HHHHHHHHHCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800