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The map label for this gene is mepA

Identifier: 16272161

GI number: 16272161

Start: 213175

End: 214035

Strand: Direct

Name: mepA

Synonym: HI0197

Alternate gene names: 16272161

Gene position: 213175-214035 (Clockwise)

Preceding gene: 16272160

Following gene: 16272162

Centisome position: 11.65

GC content: 43.55

Gene sequence:

>861_bases
ATGAATAAAATTTTATTAAAAACAACCATAATTTTTACCGCACTTTTCTCTCTGAACGTTGTTGCGTCTCCTCTAGATTG
GCAAAAAGTGAAACGCCCTATTCCAAGTGAAGATGGTAAAGCAAGTCCTATTGGTAGTTATACCAACGGGTGTATTATTG
GAGCACAAGCATTACCGCCGAAAGGTGAAGGTTACCAAGTGATTCGCATGAATCGCAATCGTTATTATGGTCATCCAAAT
ATGATTCAGTATCTTGAACGTTTGGGGCAGCGAGCTAAAGCTGCTGGGTTGCCAACGATGTTAGTAGGCGATATTGCCAT
GCCCGGTGGTGGGCGGTTTTTAACGGGTCATGCTAGCCATCAGATGGGATTAGATGCGGATATTTGGTTAAGAATGGGCG
AAATGTCAGATGCCGATGCGCTCAATTCTGATGGTAAAGGTTTATTAGTTGTGGATAGAAAGGCGCAACGAGTAGATGAG
CGCGTGTGGAATAGCAATCATGCGACGTTAATCAAGTTAGCTGCGCAAGATCCAAATGTGACGCGTATTTTTGTTAATCC
TGCGATTAAGGTAAAATTATGTCAAACCGCTGGCAATGATCGCGGTTGGCTACACAAAATTCGTCCTTGGCATGGACACA
ACTCTCATTTTCATGTTCGTTTAACTTGCCCAGCAGATGCATCTTATTGTGAAAATCAAGCGCCTGTGCCTGCTGGTGAT
GGTTGTGGAGACGAATTATATTCTTGGTTTGAACCGCCAAAACCAGGCACTTCAGTGAGCAAACCTAAAGTTACACCGCC
TGAGCCGTTTTTGTGCCAACAGATTTTGAACTCACCGAATCGGAGAGAATGGTTAGAATAG

Upstream 100 bases:

>100_bases
GGCACAGTGTAAATAATCCTGTACCTAGGGTAGTATACTCTAGGTTAGTTCTTCAATAGGACTGAACCTAGGATATACTT
ACCTACAGCAAAACTTTAGA

Downstream 100 bases:

>100_bases
CATTGAGGTAAATCAATATGGATATCGGCATTGATCTTTTAGCAATATTGTTTTGTGTTGGTTTTGTCGCATCATTTATC
GATGCAATTGCTGGCGGTGG

Product: penicillin-insensitive murein endopeptidase

Products: Split Peptidoglycan Strands [C]

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MNKILLKTTIIFTALFSLNVVASPLDWQKVKRPIPSEDGKASPIGSYTNGCIIGAQALPPKGEGYQVIRMNRNRYYGHPN
MIQYLERLGQRAKAAGLPTMLVGDIAMPGGGRFLTGHASHQMGLDADIWLRMGEMSDADALNSDGKGLLVVDRKAQRVDE
RVWNSNHATLIKLAAQDPNVTRIFVNPAIKVKLCQTAGNDRGWLHKIRPWHGHNSHFHVRLTCPADASYCENQAPVPAGD
GCGDELYSWFEPPKPGTSVSKPKVTPPEPFLCQQILNSPNRREWLE

Sequences:

>Translated_286_residues
MNKILLKTTIIFTALFSLNVVASPLDWQKVKRPIPSEDGKASPIGSYTNGCIIGAQALPPKGEGYQVIRMNRNRYYGHPN
MIQYLERLGQRAKAAGLPTMLVGDIAMPGGGRFLTGHASHQMGLDADIWLRMGEMSDADALNSDGKGLLVVDRKAQRVDE
RVWNSNHATLIKLAAQDPNVTRIFVNPAIKVKLCQTAGNDRGWLHKIRPWHGHNSHFHVRLTCPADASYCENQAPVPAGD
GCGDELYSWFEPPKPGTSVSKPKVTPPEPFLCQQILNSPNRREWLE
>Mature_286_residues
MNKILLKTTIIFTALFSLNVVASPLDWQKVKRPIPSEDGKASPIGSYTNGCIIGAQALPPKGEGYQVIRMNRNRYYGHPN
MIQYLERLGQRAKAAGLPTMLVGDIAMPGGGRFLTGHASHQMGLDADIWLRMGEMSDADALNSDGKGLLVVDRKAQRVDE
RVWNSNHATLIKLAAQDPNVTRIFVNPAIKVKLCQTAGNDRGWLHKIRPWHGHNSHFHVRLTCPADASYCENQAPVPAGD
GCGDELYSWFEPPKPGTSVSKPKVTPPEPFLCQQILNSPNRREWLE

Specific function: Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein

COG id: COG3770

COG function: function code M; Murein endopeptidase

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M74 family

Homologues:

Organism=Escherichia coli, GI1788668, Length=251, Percent_Identity=50.996015936255, Blast_Score=272, Evalue=2e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEPA_HAEIN (P44566)

Other databases:

- EMBL:   L42023
- PIR:   H64053
- RefSeq:   NP_438366.1
- ProteinModelPortal:   P44566
- SMR:   P44566
- MEROPS:   M74.001
- GeneID:   951103
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0197
- NMPDR:   fig|71421.1.peg.187
- TIGR:   HI_0197
- HOGENOM:   HBG296858
- OMA:   RIFVNAA
- ProtClustDB:   PRK09429
- BioCyc:   HINF71421:HI_0197-MONOMER
- GO:   GO:0006508
- InterPro:   IPR009045
- InterPro:   IPR005073
- Gene3D:   G3DSA:3.30.1380.10
- PIRSF:   PIRSF018455

Pfam domain/function: PF03411 Peptidase_M74; SSF55166 Hedgehog_sig_N

EC number: 3.4.99.- [C]

Molecular weight: Translated: 31653; Mature: 31653

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKILLKTTIIFTALFSLNVVASPLDWQKVKRPIPSEDGKASPIGSYTNGCIIGAQALPP
CCCEEHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCC
KGEGYQVIRMNRNRYYGHPNMIQYLERLGQRAKAAGLPTMLVGDIAMPGGGRFLTGHASH
CCCCEEEEEECCCEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEECCCCC
QMGLDADIWLRMGEMSDADALNSDGKGLLVVDRKAQRVDERVWNSNHATLIKLAAQDPNV
CCCCCHHHEEEECCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCEEEEEEECCCCCE
TRIFVNPAIKVKLCQTAGNDRGWLHKIRPWHGHNSHFHVRLTCPADASYCENQAPVPAGD
EEEEECCCEEEEEEECCCCCCCCEEECCCCCCCCCEEEEEEECCCCHHHHCCCCCCCCCC
GCGDELYSWFEPPKPGTSVSKPKVTPPEPFLCQQILNSPNRREWLE
CCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHCCC
>Mature Secondary Structure
MNKILLKTTIIFTALFSLNVVASPLDWQKVKRPIPSEDGKASPIGSYTNGCIIGAQALPP
CCCEEHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCC
KGEGYQVIRMNRNRYYGHPNMIQYLERLGQRAKAAGLPTMLVGDIAMPGGGRFLTGHASH
CCCCEEEEEECCCEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEECCCCC
QMGLDADIWLRMGEMSDADALNSDGKGLLVVDRKAQRVDERVWNSNHATLIKLAAQDPNV
CCCCCHHHEEEECCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCEEEEEEECCCCCE
TRIFVNPAIKVKLCQTAGNDRGWLHKIRPWHGHNSHFHVRLTCPADASYCENQAPVPAGD
EEEEECCCEEEEEEECCCCCCCCEEECCCCCCCCCEEEEEEECCCCHHHHCCCCCCCCCC
GCGDELYSWFEPPKPGTSVSKPKVTPPEPFLCQQILNSPNRREWLE
CCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond [C]

Specific reaction: Split The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond Connecting Neighboring Peptidoglycan Strands. [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800