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The map label for this gene is seqA

Identifier: 16272157

GI number: 16272157

Start: 206691

End: 207284

Strand: Direct

Name: seqA

Synonym: HI0192

Alternate gene names: 16272157

Gene position: 206691-207284 (Clockwise)

Preceding gene: 16272153

Following gene: 16272158

Centisome position: 11.29

GC content: 34.51

Gene sequence:

>594_bases
ATGAAGATCATTGAAGTAGATGAAGAATTATACCAATATATTGCGAGCCAAACCCGCTCTATTGGAGAAAGTGCTTCGGA
TATTTTACGCCGTTTGCTTAGTTTACCTGTGCATACTTCAATTGTTAATGATTTAATTATTACTTCAGCAGAAACAGATC
AAAAGCCAAAACAGGCTATCAATGTGAAAGAAGTAAATATCAAAACAACAAAAAAGCAATCAATAACCGCAATTAATCAG
ATTGTAGAAAAAGTCCAAACTTTATTAAATTCAACTGAATTTCAAGAAGAAAGTAAGGCTGTTGTACGTTTCTTAGCGAT
TTTACGTGTGCTTTATCGTACAAATCCTGAAAGTTTTGCGCAAGCAACGGAATCTTTACAAGGACGTACTCGAGTTTACT
TTGCACGCGATGAAGCGACATTATTAATGGCAGGTAACCATACTAAACCAAAACAAATTCCAGATACGCCTTATTGGGTT
ATTACCAATACAAATAGTGGACGAAAAATGTTAATGCTAGAAGGTGCAATGCAATCTATGGAATTACCTGAAACATTGAT
TGATGAAGTGCGATCATATTTCACGGTAAATTAA

Upstream 100 bases:

>100_bases
AAATTGATAATTGAGTAATGATTTTGCCATAATTTTTACGTGAGAATTTGTAAAGAAGTCGTTATAATTGAAAAAAATTT
TAGCAAACAGAGAGATAAAA

Downstream 100 bases:

>100_bases
TAATGTATCCTTGGCAAGATTTTGCTATTCAACCTGATTTCTCAGATAAAATCGCTTTGCGTACTACGCAGGGCGATATG
CTTACTTGGATAGAATTGAC

Product: replication initiation regulator SeqA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MKIIEVDEELYQYIASQTRSIGESASDILRRLLSLPVHTSIVNDLIITSAETDQKPKQAINVKEVNIKTTKKQSITAINQ
IVEKVQTLLNSTEFQEESKAVVRFLAILRVLYRTNPESFAQATESLQGRTRVYFARDEATLLMAGNHTKPKQIPDTPYWV
ITNTNSGRKMLMLEGAMQSMELPETLIDEVRSYFTVN

Sequences:

>Translated_197_residues
MKIIEVDEELYQYIASQTRSIGESASDILRRLLSLPVHTSIVNDLIITSAETDQKPKQAINVKEVNIKTTKKQSITAINQ
IVEKVQTLLNSTEFQEESKAVVRFLAILRVLYRTNPESFAQATESLQGRTRVYFARDEATLLMAGNHTKPKQIPDTPYWV
ITNTNSGRKMLMLEGAMQSMELPETLIDEVRSYFTVN
>Mature_197_residues
MKIIEVDEELYQYIASQTRSIGESASDILRRLLSLPVHTSIVNDLIITSAETDQKPKQAINVKEVNIKTTKKQSITAINQ
IVEKVQTLLNSTEFQEESKAVVRFLAILRVLYRTNPESFAQATESLQGRTRVYFARDEATLLMAGNHTKPKQIPDTPYWV
ITNTNSGRKMLMLEGAMQSMELPETLIDEVRSYFTVN

Specific function: Negative regulator of replication initiation. Required for sequestration, a process that blocks secondary initiation events. Could block the allosteric transition of dnaA from an inactive form to an active form

COG id: COG3057

COG function: function code L; Negative regulator of replication initiationR

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786903, Length=193, Percent_Identity=49.2227979274611, Blast_Score=178, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SEQA_HAEIN (P44564)

Other databases:

- EMBL:   L42023
- PIR:   D64053
- RefSeq:   NP_438362.1
- ProteinModelPortal:   P44564
- GeneID:   951105
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0192
- TIGR:   HI_0192
- HOGENOM:   HBG296989
- OMA:   YRHIASK
- ProtClustDB:   PRK11187
- BioCyc:   HINF71421:HI_0192-MONOMER
- InterPro:   IPR005621
- InterPro:   IPR010985
- Gene3D:   G3DSA:1.20.1380.10

Pfam domain/function: PF03925 SeqA; SSF47598 Met_repress_like; SSF82808 Regulation_modulator_SeqA

EC number: NA

Molecular weight: Translated: 22381; Mature: 22381

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIEVDEELYQYIASQTRSIGESASDILRRLLSLPVHTSIVNDLIITSAETDQKPKQAI
CCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHC
NVKEVNIKTTKKQSITAINQIVEKVQTLLNSTEFQEESKAVVRFLAILRVLYRTNPESFA
CEEEEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
QATESLQGRTRVYFARDEATLLMAGNHTKPKQIPDTPYWVITNTNSGRKMLMLEGAMQSM
HHHHHHCCCEEEEEEECCEEEEEECCCCCCCCCCCCCEEEEEECCCCCEEEEEHHHHHHC
ELPETLIDEVRSYFTVN
CCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MKIIEVDEELYQYIASQTRSIGESASDILRRLLSLPVHTSIVNDLIITSAETDQKPKQAI
CCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHC
NVKEVNIKTTKKQSITAINQIVEKVQTLLNSTEFQEESKAVVRFLAILRVLYRTNPESFA
CEEEEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
QATESLQGRTRVYFARDEATLLMAGNHTKPKQIPDTPYWVITNTNSGRKMLMLEGAMQSM
HHHHHHCCCEEEEEEECCEEEEEECCCCCCCCCCCCCEEEEEECCCCCEEEEEHHHHHHC
ELPETLIDEVRSYFTVN
CCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800