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The map label for this gene is menE

Identifier: 16272158

GI number: 16272158

Start: 207287

End: 208645

Strand: Direct

Name: menE

Synonym: HI0194

Alternate gene names: 16272158

Gene position: 207287-208645 (Clockwise)

Preceding gene: 16272157

Following gene: 16272159

Centisome position: 11.33

GC content: 36.42

Gene sequence:

>1359_bases
ATGTATCCTTGGCAAGATTTTGCTATTCAACCTGATTTCTCAGATAAAATCGCTTTGCGTACTACGCAGGGCGATATGCT
TACTTGGATAGAATTGACTACAAAGATTAACCAAACAGTGGCTTTTTTACAAAAAAAAGGCGTAAATGCAGAAAGTGCGG
TTGCTTTTGTGGGAAAAAATTCAGAGAAAATTTTATTTTTATATCTGGCGACAATTCAGCTTGGCGCAAAAGTTTTAGGC
ATAAACCCTGCTTTTCCACAAGAAAAAATTGCAAAATTATGTGAGTTTTATCAAATTGATTTTTGTTTTTATGATAAAGA
TTTACTGAATTTGCAAGAAATTGATGTTTTTACTCAAAAAGCCGATTTTTTTCGTCCTGCGACGATGACGCTAACGTCTG
GCTCGACAGGTTTACCAAAAGCAGTTGTGCATAATGTCCAAGCACATTTGGATAATGCAAAAGGGGTATGTAACTTAATG
AAGTTTGATTGTAATCAATCTTGGTTACTTTCATTACCCTTATATCACGTTTCAGGGCAAGGTATTGTTTGGCGTTGGTT
ATATTGCGGTGCACAATTACATTTCCCAGAAGATGATTTTTATGCTTCATTATTAAAGACGACCCACGTTTCTCTTGTGC
CAACGCAATTACAGCGTTTATTAGATTATTTACAGGAAAATCCGAGCATTTCATTTGCTACACGCCATATTTTACTGGGC
GGTGCGCATATTCCGACAGAACTTACACAAAATATGTTGAAATATGGTATCGAAACGTATTCTGGCTACGGAATGACGGA
AATGGCTTCGACAGTTTTTGCTAAAAAATCTGACAGAAAACAAGGCGTAGGGCAACCGCTCTTAGGTAGAGAGTATTGTT
TAGTAAATGATGAAATTTGGCTGAAAGGTGCAGGTTTGGCGATGGGTTATTGGAAAGATCGACAAATTGTTCCATTAACG
AATAACCAAGGCTGGATTCAGACAAAAGATAAAGGCATTTGGCAAGAGGGCGAACTTGTTATTATCGGACGACTTGATAA
TATGTTTATTTCAGGTGGCGAAAATATTCAGCCAGAAGAAATTGAACAAGTGATTATTCAACATTCTTCAGTTAATCAAG
TATTTGTTTTACCACAAAAAAACAAAGAATTTGGTCAGCGTCCTGTCGCTTTAGTGGATTTTAATGAGCCCTTTAGCAAA
AGTGCGGTTGAAAATTTAATGTTTTTTTTACAAGATAAACTTGCACGTTTTAAACAACCTATTGCATATTATCCTTTGCC
ACTGATGCTTGAGAAAGGCATTAAGATCTCACGTAAACAGCTTGCTGATTGGTTGGCAAAGCGAGATGAGATAAATTAA

Upstream 100 bases:

>100_bases
GTGGACGAAAAATGTTAATGCTAGAAGGTGCAATGCAATCTATGGAATTACCTGAAACATTGATTGATGAAGTGCGATCA
TATTTCACGGTAAATTAATA

Downstream 100 bases:

>100_bases
TTTACCATTTAGATTAGGGAAAAATAATGATTAGGAAACTTATGAAAATACCTCCATTTTTTACCGCACTTTTTGCCTCG
GCAATGTTTACCCTTAGCGT

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MYPWQDFAIQPDFSDKIALRTTQGDMLTWIELTTKINQTVAFLQKKGVNAESAVAFVGKNSEKILFLYLATIQLGAKVLG
INPAFPQEKIAKLCEFYQIDFCFYDKDLLNLQEIDVFTQKADFFRPATMTLTSGSTGLPKAVVHNVQAHLDNAKGVCNLM
KFDCNQSWLLSLPLYHVSGQGIVWRWLYCGAQLHFPEDDFYASLLKTTHVSLVPTQLQRLLDYLQENPSISFATRHILLG
GAHIPTELTQNMLKYGIETYSGYGMTEMASTVFAKKSDRKQGVGQPLLGREYCLVNDEIWLKGAGLAMGYWKDRQIVPLT
NNQGWIQTKDKGIWQEGELVIIGRLDNMFISGGENIQPEEIEQVIIQHSSVNQVFVLPQKNKEFGQRPVALVDFNEPFSK
SAVENLMFFLQDKLARFKQPIAYYPLPLMLEKGIKISRKQLADWLAKRDEIN

Sequences:

>Translated_452_residues
MYPWQDFAIQPDFSDKIALRTTQGDMLTWIELTTKINQTVAFLQKKGVNAESAVAFVGKNSEKILFLYLATIQLGAKVLG
INPAFPQEKIAKLCEFYQIDFCFYDKDLLNLQEIDVFTQKADFFRPATMTLTSGSTGLPKAVVHNVQAHLDNAKGVCNLM
KFDCNQSWLLSLPLYHVSGQGIVWRWLYCGAQLHFPEDDFYASLLKTTHVSLVPTQLQRLLDYLQENPSISFATRHILLG
GAHIPTELTQNMLKYGIETYSGYGMTEMASTVFAKKSDRKQGVGQPLLGREYCLVNDEIWLKGAGLAMGYWKDRQIVPLT
NNQGWIQTKDKGIWQEGELVIIGRLDNMFISGGENIQPEEIEQVIIQHSSVNQVFVLPQKNKEFGQRPVALVDFNEPFSK
SAVENLMFFLQDKLARFKQPIAYYPLPLMLEKGIKISRKQLADWLAKRDEIN
>Mature_452_residues
MYPWQDFAIQPDFSDKIALRTTQGDMLTWIELTTKINQTVAFLQKKGVNAESAVAFVGKNSEKILFLYLATIQLGAKVLG
INPAFPQEKIAKLCEFYQIDFCFYDKDLLNLQEIDVFTQKADFFRPATMTLTSGSTGLPKAVVHNVQAHLDNAKGVCNLM
KFDCNQSWLLSLPLYHVSGQGIVWRWLYCGAQLHFPEDDFYASLLKTTHVSLVPTQLQRLLDYLQENPSISFATRHILLG
GAHIPTELTQNMLKYGIETYSGYGMTEMASTVFAKKSDRKQGVGQPLLGREYCLVNDEIWLKGAGLAMGYWKDRQIVPLT
NNQGWIQTKDKGIWQEGELVIIGRLDNMFISGGENIQPEEIEQVIIQHSSVNQVFVLPQKNKEFGQRPVALVDFNEPFSK
SAVENLMFFLQDKLARFKQPIAYYPLPLMLEKGIKISRKQLADWLAKRDEIN

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA)

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily

Homologues:

Organism=Homo sapiens, GI156151445, Length=346, Percent_Identity=25.1445086705202, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788595, Length=458, Percent_Identity=43.6681222707424, Blast_Score=347, Evalue=7e-97,
Organism=Escherichia coli, GI145693145, Length=513, Percent_Identity=22.2222222222222, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788107, Length=323, Percent_Identity=26.0061919504644, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI32563687, Length=450, Percent_Identity=26.6666666666667, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17558820, Length=471, Percent_Identity=27.8131634819533, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71994703, Length=350, Percent_Identity=25.1428571428571, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71994690, Length=350, Percent_Identity=25.1428571428571, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71994694, Length=350, Percent_Identity=25.1428571428571, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17531443, Length=326, Percent_Identity=26.6871165644172, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17560308, Length=326, Percent_Identity=25.7668711656442, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17559526, Length=333, Percent_Identity=25.2252252252252, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=348, Percent_Identity=25.5747126436782, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21355181, Length=447, Percent_Identity=22.1476510067114, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MENE_HAEIN (P44565)

Other databases:

- EMBL:   L42023
- PIR:   F64053
- RefSeq:   NP_438363.1
- ProteinModelPortal:   P44565
- SMR:   P44565
- GeneID:   951106
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0194
- NMPDR:   fig|71421.1.peg.184
- TIGR:   HI_0194
- HOGENOM:   HBG547964
- OMA:   VACISIN
- ProtClustDB:   PRK09029
- BioCyc:   HINF71421:HI_0194-MONOMER
- BRENDA:   6.2.1.26
- HAMAP:   MF_00731
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- TIGRFAMs:   TIGR01923

Pfam domain/function: PF00501 AMP-binding

EC number: =6.2.1.26

Molecular weight: Translated: 51395; Mature: 51395

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00455 AMP_BINDING; PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYPWQDFAIQPDFSDKIALRTTQGDMLTWIELTTKINQTVAFLQKKGVNAESAVAFVGKN
CCCCCCCEECCCCCCCEEEEECCCCEEEEEHHHHHHHHHHHHHHHCCCCHHHEEEEECCC
SEKILFLYLATIQLGAKVLGINPAFPQEKIAKLCEFYQIDFCFYDKDLLNLQEIDVFTQK
CCEEEEEEEEHHHHCCEEEECCCCCCHHHHHHHHHHHEEEEEEECCHHCCHHHHHHHHHH
ADFFRPATMTLTSGSTGLPKAVVHNVQAHLDNAKGVCNLMKFDCNQSWLLSLPLYHVSGQ
HHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEHHEECCCCCEEEEECEEEECCC
GIVWRWLYCGAQLHFPEDDFYASLLKTTHVSLVPTQLQRLLDYLQENPSISFATRHILLG
CEEEEEEEECCEEECCCHHHHHHHHHHHHHEECHHHHHHHHHHHHCCCCEEEEEEEEEEC
GAHIPTELTQNMLKYGIETYSGYGMTEMASTVFAKKSDRKQGVGQPLLGREYCLVNDEIW
CCCCCHHHHHHHHHHCHHHHCCCCHHHHHHHHHHCCCCHHCCCCCCCCCCEEEEECCCEE
LKGAGLAMGYWKDRQIVPLTNNQGWIQTKDKGIWQEGELVIIGRLDNMFISGGENIQPEE
EEECCEEEEECCCCEEEEEECCCCEEEECCCCCCCCCCEEEEEECCCEEECCCCCCCHHH
IEQVIIQHSSVNQVFVLPQKNKEFGQRPVALVDFNEPFSKSAVENLMFFLQDKLARFKQP
HHHHHHHCCCCCEEEEEECCCHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCC
IAYYPLPLMLEKGIKISRKQLADWLAKRDEIN
HHCCCCHHHHHCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MYPWQDFAIQPDFSDKIALRTTQGDMLTWIELTTKINQTVAFLQKKGVNAESAVAFVGKN
CCCCCCCEECCCCCCCEEEEECCCCEEEEEHHHHHHHHHHHHHHHCCCCHHHEEEEECCC
SEKILFLYLATIQLGAKVLGINPAFPQEKIAKLCEFYQIDFCFYDKDLLNLQEIDVFTQK
CCEEEEEEEEHHHHCCEEEECCCCCCHHHHHHHHHHHEEEEEEECCHHCCHHHHHHHHHH
ADFFRPATMTLTSGSTGLPKAVVHNVQAHLDNAKGVCNLMKFDCNQSWLLSLPLYHVSGQ
HHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEHHEECCCCCEEEEECEEEECCC
GIVWRWLYCGAQLHFPEDDFYASLLKTTHVSLVPTQLQRLLDYLQENPSISFATRHILLG
CEEEEEEEECCEEECCCHHHHHHHHHHHHHEECHHHHHHHHHHHHCCCCEEEEEEEEEEC
GAHIPTELTQNMLKYGIETYSGYGMTEMASTVFAKKSDRKQGVGQPLLGREYCLVNDEIW
CCCCCHHHHHHHHHHCHHHHCCCCHHHHHHHHHHCCCCHHCCCCCCCCCCEEEEECCCEE
LKGAGLAMGYWKDRQIVPLTNNQGWIQTKDKGIWQEGELVIIGRLDNMFISGGENIQPEE
EEECCEEEEECCCCEEEEEECCCCEEEECCCCCCCCCCEEEEEECCCEEECCCCCCCHHH
IEQVIIQHSSVNQVFVLPQKNKEFGQRPVALVDFNEPFSKSAVENLMFFLQDKLARFKQP
HHHHHHHCCCCCEEEEEECCCHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCC
IAYYPLPLMLEKGIKISRKQLADWLAKRDEIN
HHCCCCHHHHHCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800