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The map label for this gene is pflB [H]

Identifier: 16272145

GI number: 16272145

Start: 192211

End: 194529

Strand: Reverse

Name: pflB [H]

Synonym: HI0180

Alternate gene names: 16272145

Gene position: 194529-192211 (Counterclockwise)

Preceding gene: 16272146

Following gene: 16272144

Centisome position: 10.63

GC content: 39.41

Gene sequence:

>2319_bases
ATGACTATGTCAGAACTTAATGAAATGCAAAAATTGGCGTGGGCTGGTTTTGCTGGTGGCGATTGGCAAGAAAATGTCAA
TGTACGTGACTTTATCCAAAAAAACTATACCCCTTATGAAGGCGATGACTCTTTCTTAGCAGGTCCAACCGAAGCAACAA
CCAAGCTTTGGGAATCTGTGATGGAAGGTATTAAAATTGAAAACCGTACTCACGCGCCATTAGATTTTGATGAACATACA
CCATCTACCATTATCTCTCACGCACCTGGTTACATTAACAAAGATTTAGAAAAAATCGTTGGTCTTCAAACTGATGAACC
TTTAAAACGTGCCATTATGCCATTCGGTGGTATCAAAATGGTGGAAGGTTCTTGTAAAGTTTATGGTCGTGAACTTGATC
CAAAAGTGAAAAAAATCTTCACTGAATACCGTAAAACACATAACCAAGGTGTATTCGATGTTTACACGCCAGATATTTTA
CGTTGCCGTAAATCTGGGGTATTAACTGGTCTTCCAGATGCTTATGGTCGTGGTCGTATCATCGGTGACTACCGTCGTGT
AGCACTTTATGGTGTAGATTTCTTAATGAAAGATAAATACGCACAATTCTCTTCTTTACAAAAAGATTTAGAAGATGGCG
TAAATCTTGAAGCAACAATTCGTTTACGTGAAGAAATCGCAGAACAACACCGTGCATTAGGTCAATTAAAACAAATGGCA
GCAAGCTATGGTTATGATATTTCTAACCCAGCAACTAATGCTCAAGAAGCCATTCAATGGATGTACTTTGCTTATCTTGC
TGCAATAAAATCACAAAATGGTGCTGCAATGTCATTCGGTCGTACCGCAACCTTTATTGACGTGTACATCGAACGTGATT
TAAAAGCAGGAAAAATTACTGAAACTGAAGCGCAAGAATTAGTTGACCACTTAGTTATGAAACTTCGTATGGTTCGTTTC
TTACGTACACCTGAATACGATCAATTATTCTCTGGTGACCCAATGTGGGCAACTGAAACCATCGCAGGTATGGGTTTAGA
TGGTCGTACATTAGTAACCAAAAATACATTCCGTATTTTACACACCCTTTACAACATGGGTACTTCTCCAGAGCCAAACT
TAACCATTCTTTGGTCTGAACAATTACCTGAAAACTTCAAACGTTTCTGTGCAAAAGTATCGATTGATACCTCATCAGTT
CAATACGAAAACGATGATTTAATGCGTCCAGACTTCAACAACGATGACTACGCAATCGCATGTTGTGTATCACCAATGAT
TGTGGGTAAACAAATGCAATTCTTCGGTGCACGTGCAAACTTAGCGAAAACATTGTTATACGCAATCAACGGCGGTATCG
ATGAAAAATTAGGTATGCAAGTAGGTCCGAAAACTGCACCAATTACTGATGAAGTATTAGATTTCGATACAGTAATGACT
CGTATGGATAGCTTTATGGATTGGTTGGCAAAACAATATGTGACTGCCTTAAACGTAATCCACTATATGCACGATAAATA
TTCATACGAAGCCGCATTAATGGCATTACATGATCGTGATGTATACCGTACTATGGCTTGTGGTATCGCAGGTCTTTCTG
TTGCGGCTGACTCACTTTCAGCAATCAAATATGCGAAAGTTAAACCAGTTCGTGGCGACATCAAAGATAAAGATGGCAAT
GTTGTTGCAACTAACGTAGCAATCGACTTTGAAATCGAAGGTGAATATCCACAATATGGTAACAATGATAACCGTGTTGA
TGACATCGCTTGTGACTTAGTTGAACGTTTTATGAAGAAAATTCAAAAACTTAAAACTTACCGCAATGCAGTGCCTACAC
AATCTGTATTAACCATTACTTCTAACGTAGTTTATGGTAAGAAAACGGGTAACACCCCTGATGGTCGTCGTGCTGGTGCA
CCATTCGGACCAGGTGCGAACCCAATGCACGGTCGTGACCAAAAAGGTGCGGTAGCATCATTAACTTCTGTAGCTAAACT
TCCATTTGCTTACGCGAAAGATGGTATTTCTTATACCTTCTCAATCGTACCAAATGCCTTAGGTAAAGATGCAGAAGCAC
AACGTCGCAACCTTGCTGGCTTAATGGATGGTTACTTCCACCACGAAGCAACGGTTGAAGGTGGCCAACACTTAAATGTG
AACGTGTTAAACCGTGAAATGTTATTAGATGCAATGGAAAATCCGGATAAATATCCACAATTGACTATCCGTGTATCAGG
TTACGCAGTACGTTTTAACTCTTTAACTAAAGAGCAACAACAAGACGTAATTACTAGAACTTTCACAGAGTCAATGTAA

Upstream 100 bases:

>100_bases
AACGTTGGGAAATATTGTCGGCGGGGCAATTTGTATTGGTGTATTCCAACGTTATTTAACCAAAACACATTAAAGATTTA
TTTATTAATTAACAAAGGAA

Downstream 100 bases:

>100_bases
AAACATGTTTATTTTTGACCGCACTTTGGCGGGAACATAATATGTATGAGCCTGATTTAAATCAGGCTCATTTTTTATAG
ACTAAATCTTTGGTAAAATT

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 772; Mature: 771

Protein sequence:

>772_residues
MTMSELNEMQKLAWAGFAGGDWQENVNVRDFIQKNYTPYEGDDSFLAGPTEATTKLWESVMEGIKIENRTHAPLDFDEHT
PSTIISHAPGYINKDLEKIVGLQTDEPLKRAIMPFGGIKMVEGSCKVYGRELDPKVKKIFTEYRKTHNQGVFDVYTPDIL
RCRKSGVLTGLPDAYGRGRIIGDYRRVALYGVDFLMKDKYAQFSSLQKDLEDGVNLEATIRLREEIAEQHRALGQLKQMA
ASYGYDISNPATNAQEAIQWMYFAYLAAIKSQNGAAMSFGRTATFIDVYIERDLKAGKITETEAQELVDHLVMKLRMVRF
LRTPEYDQLFSGDPMWATETIAGMGLDGRTLVTKNTFRILHTLYNMGTSPEPNLTILWSEQLPENFKRFCAKVSIDTSSV
QYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTLLYAINGGIDEKLGMQVGPKTAPITDEVLDFDTVMT
RMDSFMDWLAKQYVTALNVIHYMHDKYSYEAALMALHDRDVYRTMACGIAGLSVAADSLSAIKYAKVKPVRGDIKDKDGN
VVATNVAIDFEIEGEYPQYGNNDNRVDDIACDLVERFMKKIQKLKTYRNAVPTQSVLTITSNVVYGKKTGNTPDGRRAGA
PFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDAEAQRRNLAGLMDGYFHHEATVEGGQHLNV
NVLNREMLLDAMENPDKYPQLTIRVSGYAVRFNSLTKEQQQDVITRTFTESM

Sequences:

>Translated_772_residues
MTMSELNEMQKLAWAGFAGGDWQENVNVRDFIQKNYTPYEGDDSFLAGPTEATTKLWESVMEGIKIENRTHAPLDFDEHT
PSTIISHAPGYINKDLEKIVGLQTDEPLKRAIMPFGGIKMVEGSCKVYGRELDPKVKKIFTEYRKTHNQGVFDVYTPDIL
RCRKSGVLTGLPDAYGRGRIIGDYRRVALYGVDFLMKDKYAQFSSLQKDLEDGVNLEATIRLREEIAEQHRALGQLKQMA
ASYGYDISNPATNAQEAIQWMYFAYLAAIKSQNGAAMSFGRTATFIDVYIERDLKAGKITETEAQELVDHLVMKLRMVRF
LRTPEYDQLFSGDPMWATETIAGMGLDGRTLVTKNTFRILHTLYNMGTSPEPNLTILWSEQLPENFKRFCAKVSIDTSSV
QYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTLLYAINGGIDEKLGMQVGPKTAPITDEVLDFDTVMT
RMDSFMDWLAKQYVTALNVIHYMHDKYSYEAALMALHDRDVYRTMACGIAGLSVAADSLSAIKYAKVKPVRGDIKDKDGN
VVATNVAIDFEIEGEYPQYGNNDNRVDDIACDLVERFMKKIQKLKTYRNAVPTQSVLTITSNVVYGKKTGNTPDGRRAGA
PFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDAEAQRRNLAGLMDGYFHHEATVEGGQHLNV
NVLNREMLLDAMENPDKYPQLTIRVSGYAVRFNSLTKEQQQDVITRTFTESM
>Mature_771_residues
TMSELNEMQKLAWAGFAGGDWQENVNVRDFIQKNYTPYEGDDSFLAGPTEATTKLWESVMEGIKIENRTHAPLDFDEHTP
STIISHAPGYINKDLEKIVGLQTDEPLKRAIMPFGGIKMVEGSCKVYGRELDPKVKKIFTEYRKTHNQGVFDVYTPDILR
CRKSGVLTGLPDAYGRGRIIGDYRRVALYGVDFLMKDKYAQFSSLQKDLEDGVNLEATIRLREEIAEQHRALGQLKQMAA
SYGYDISNPATNAQEAIQWMYFAYLAAIKSQNGAAMSFGRTATFIDVYIERDLKAGKITETEAQELVDHLVMKLRMVRFL
RTPEYDQLFSGDPMWATETIAGMGLDGRTLVTKNTFRILHTLYNMGTSPEPNLTILWSEQLPENFKRFCAKVSIDTSSVQ
YENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTLLYAINGGIDEKLGMQVGPKTAPITDEVLDFDTVMTR
MDSFMDWLAKQYVTALNVIHYMHDKYSYEAALMALHDRDVYRTMACGIAGLSVAADSLSAIKYAKVKPVRGDIKDKDGNV
VATNVAIDFEIEGEYPQYGNNDNRVDDIACDLVERFMKKIQKLKTYRNAVPTQSVLTITSNVVYGKKTGNTPDGRRAGAP
FGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDAEAQRRNLAGLMDGYFHHEATVEGGQHLNVN
VLNREMLLDAMENPDKYPQLTIRVSGYAVRFNSLTKEQQQDVITRTFTESM

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=770, Percent_Identity=85.1948051948052, Blast_Score=1393, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=760, Percent_Identity=75.2631578947368, Blast_Score=1239, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=585, Percent_Identity=27.5213675213675, Blast_Score=186, Evalue=6e-48,
Organism=Escherichia coli, GI1790388, Length=544, Percent_Identity=25.7352941176471, Blast_Score=143, Evalue=4e-35,
Organism=Escherichia coli, GI1788933, Length=64, Percent_Identity=79.6875, Blast_Score=108, Evalue=1e-24,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 86532; Mature: 86401

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMSELNEMQKLAWAGFAGGDWQENVNVRDFIQKNYTPYEGDDSFLAGPTEATTKLWESV
CCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHH
MEGIKIENRTHAPLDFDEHTPSTIISHAPGYINKDLEKIVGLQTDEPLKRAIMPFGGIKM
HHCCEECCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCEEE
VEGSCKVYGRELDPKVKKIFTEYRKTHNQGVFDVYTPDILRCRKSGVLTGLPDAYGRGRI
ECCCCEEECCCCCHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHCCCCCCCCCCCCCCCE
IGDYRRVALYGVDFLMKDKYAQFSSLQKDLEDGVNLEATIRLREEIAEQHRALGQLKQMA
EHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
ASYGYDISNPATNAQEAIQWMYFAYLAAIKSQNGAAMSFGRTATFIDVYIERDLKAGKIT
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEEEEECCCCCCCCC
ETEAQELVDHLVMKLRMVRFLRTPEYDQLFSGDPMWATETIAGMGLDGRTLVTKNTFRIL
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHCCCCCCCEEEEHHHHHHH
HTLYNMGTSPEPNLTILWSEQLPENFKRFCAKVSIDTSSVQYENDDLMRPDFNNDDYAIA
HHHHHCCCCCCCCEEEEECHHCCHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCEEE
CCVSPMIVGKQMQFFGARANLAKTLLYAINGGIDEKLGMQVGPKTAPITDEVLDFDTVMT
EECCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHH
RMDSFMDWLAKQYVTALNVIHYMHDKYSYEAALMALHDRDVYRTMACGIAGLSVAADSLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIKYAKVKPVRGDIKDKDGNVVATNVAIDFEIEGEYPQYGNNDNRVDDIACDLVERFMKK
HHHHHCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHH
IQKLKTYRNAVPTQSVLTITSNVVYGKKTGNTPDGRRAGAPFGPGANPMHGRDQKGAVAS
HHHHHHHHHCCCCCHHEEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
LTSVAKLPFAYAKDGISYTFSIVPNALGKDAEAQRRNLAGLMDGYFHHEATVEGGQHLNV
HHHHHHCCHHHHCCCCCEEEEECCHHCCCCHHHHHHHHHHHHHHHHHCHHEECCCCEEEE
NVLNREMLLDAMENPDKYPQLTIRVSGYAVRFNSLTKEQQQDVITRTFTESM
EEHHHHHHHHHHCCCCCCCEEEEEEECEEEEEHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TMSELNEMQKLAWAGFAGGDWQENVNVRDFIQKNYTPYEGDDSFLAGPTEATTKLWESV
CHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHH
MEGIKIENRTHAPLDFDEHTPSTIISHAPGYINKDLEKIVGLQTDEPLKRAIMPFGGIKM
HHCCEECCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCEEE
VEGSCKVYGRELDPKVKKIFTEYRKTHNQGVFDVYTPDILRCRKSGVLTGLPDAYGRGRI
ECCCCEEECCCCCHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHCCCCCCCCCCCCCCCE
IGDYRRVALYGVDFLMKDKYAQFSSLQKDLEDGVNLEATIRLREEIAEQHRALGQLKQMA
EHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
ASYGYDISNPATNAQEAIQWMYFAYLAAIKSQNGAAMSFGRTATFIDVYIERDLKAGKIT
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEEEEECCCCCCCCC
ETEAQELVDHLVMKLRMVRFLRTPEYDQLFSGDPMWATETIAGMGLDGRTLVTKNTFRIL
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHCCCCCCCEEEEHHHHHHH
HTLYNMGTSPEPNLTILWSEQLPENFKRFCAKVSIDTSSVQYENDDLMRPDFNNDDYAIA
HHHHHCCCCCCCCEEEEECHHCCHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCEEE
CCVSPMIVGKQMQFFGARANLAKTLLYAINGGIDEKLGMQVGPKTAPITDEVLDFDTVMT
EECCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHH
RMDSFMDWLAKQYVTALNVIHYMHDKYSYEAALMALHDRDVYRTMACGIAGLSVAADSLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIKYAKVKPVRGDIKDKDGNVVATNVAIDFEIEGEYPQYGNNDNRVDDIACDLVERFMKK
HHHHHCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHH
IQKLKTYRNAVPTQSVLTITSNVVYGKKTGNTPDGRRAGAPFGPGANPMHGRDQKGAVAS
HHHHHHHHHCCCCCHHEEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
LTSVAKLPFAYAKDGISYTFSIVPNALGKDAEAQRRNLAGLMDGYFHHEATVEGGQHLNV
HHHHHHCCHHHHCCCCCEEEEECCHHCCCCHHHHHHHHHHHHHHHHHCHHEECCCCEEEE
NVLNREMLLDAMENPDKYPQLTIRVSGYAVRFNSLTKEQQQDVITRTFTESM
EEHHHHHHHHHHCCCCCCCEEEEEEECEEEEEHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]