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The map label for this gene is pflA

Identifier: 16272144

GI number: 16272144

Start: 191340

End: 192080

Strand: Reverse

Name: pflA

Synonym: HI0179

Alternate gene names: 16272144

Gene position: 192080-191340 (Counterclockwise)

Preceding gene: 16272145

Following gene: 16272143

Centisome position: 10.5

GC content: 36.71

Gene sequence:

>741_bases
ATGTCAGTTCTTGGACGAATTCACTCTTTTGAATCCTGTGGCACTGTAGATGGGCCAGGTATTCGTTTTATTTTATTTAT
GCAAGGCTGCTTGATGCGCTGCAAATATTGCCACAATCGTGATACTTGGGATCTTGAAGGTGGTAAAGAAATCAGTGTCG
AAGATTTAATGAAAGAAGTCGTGACTTATCGCCATTTTATGAATGCTACTGGCGGTGGTGTCACAGCATCTGGTGGCGAG
GCTGTGTTACAAGCAGAGTTTGTACGCGATTGGTTCCGTGCTTGTAAAGAGGAAGGGATTAATACTTGCTTAGATACAAA
TGGTTTTGTACGTCATTATGATCATATTATTGATGAATTATTAGATGTAACAGATCTTGTTTTACTTGATTTAAAAGAAC
TTAATGATCAAGTTCATCAAAATCTTATTGGGGTGCCAAATAAACGTACCCTTGAATTTGCAAAATATTTGCAAAAACGT
AATCAACATACCTGGATTCGTTATGTTGTGGTTCCTGGTTATACTGATAGCGATCACGATGTGCATTTATTAGGTCAGTT
TATTGAAGGTATGACCAATATTGAAAAAGTTGAACTTCTTCCTTATCATCGATTAGGTGTGCATAAATGGAAAACCCTTG
GGTTAGATTATGAGCTTGAAAATGTATTACCGCCAACTAAAGAATCCTTAGAACATATTAAAACAATCCTAGAAGGTTAT
GGACACACTGTAAAATTCTAG

Upstream 100 bases:

>100_bases
GGGAACATAATATGTATGAGCCTGATTTAAATCAGGCTCATTTTTTATAGACTAAATCTTTGGTAAAATTTAGCTATATC
TAATTTTAGGAAATTTATGT

Downstream 100 bases:

>100_bases
AATAAATGTCAGCTAACATAAGGAGTAAATAATGAAAAAAATTATTTTAACATTATCACTTGGGTTACTTACCGCTTGTT
CTGCTCAAATCCAAAAGGCT

Product: pyruvate formate lyase-activating enzyme 1

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 1; PFL-activating enzyme 1

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MSVLGRIHSFESCGTVDGPGIRFILFMQGCLMRCKYCHNRDTWDLEGGKEISVEDLMKEVVTYRHFMNATGGGVTASGGE
AVLQAEFVRDWFRACKEEGINTCLDTNGFVRHYDHIIDELLDVTDLVLLDLKELNDQVHQNLIGVPNKRTLEFAKYLQKR
NQHTWIRYVVVPGYTDSDHDVHLLGQFIEGMTNIEKVELLPYHRLGVHKWKTLGLDYELENVLPPTKESLEHIKTILEGY
GHTVKF

Sequences:

>Translated_246_residues
MSVLGRIHSFESCGTVDGPGIRFILFMQGCLMRCKYCHNRDTWDLEGGKEISVEDLMKEVVTYRHFMNATGGGVTASGGE
AVLQAEFVRDWFRACKEEGINTCLDTNGFVRHYDHIIDELLDVTDLVLLDLKELNDQVHQNLIGVPNKRTLEFAKYLQKR
NQHTWIRYVVVPGYTDSDHDVHLLGQFIEGMTNIEKVELLPYHRLGVHKWKTLGLDYELENVLPPTKESLEHIKTILEGY
GHTVKF
>Mature_245_residues
SVLGRIHSFESCGTVDGPGIRFILFMQGCLMRCKYCHNRDTWDLEGGKEISVEDLMKEVVTYRHFMNATGGGVTASGGEA
VLQAEFVRDWFRACKEEGINTCLDTNGFVRHYDHIIDELLDVTDLVLLDLKELNDQVHQNLIGVPNKRTLEFAKYLQKRN
QHTWIRYVVVPGYTDSDHDVHLLGQFIEGMTNIEKVELLPYHRLGVHKWKTLGLDYELENVLPPTKESLEHIKTILEGYG
HTVKF

Specific function: Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family

Homologues:

Organism=Escherichia coli, GI1787130, Length=246, Percent_Identity=73.9837398373984, Blast_Score=397, Evalue=1e-112,
Organism=Escherichia coli, GI1790389, Length=274, Percent_Identity=25.9124087591241, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1790839, Length=256, Percent_Identity=28.90625, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI226510931, Length=171, Percent_Identity=30.4093567251462, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PFLA_HAEIN (P43751)

Other databases:

- EMBL:   L42023
- PIR:   E64052
- RefSeq:   NP_438347.1
- ProteinModelPortal:   P43751
- SMR:   P43751
- GeneID:   951086
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0179
- NMPDR:   fig|71421.1.peg.168
- TIGR:   HI_0179
- HOGENOM:   HBG554871
- OMA:   ETCGTVD
- ProtClustDB:   PRK11145
- BioCyc:   HINF71421:HI_0179-MONOMER
- BRENDA:   1.97.1.4
- GO:   GO:0005737
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197
- TIGRFAMs:   TIGR02493

Pfam domain/function: PF04055 Radical_SAM

EC number: =1.97.1.4

Molecular weight: Translated: 28236; Mature: 28104

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLGRIHSFESCGTVDGPGIRFILFMQGCLMRCKYCHNRDTWDLEGGKEISVEDLMKEV
CCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
VTYRHFMNATGGGVTASGGEAVLQAEFVRDWFRACKEEGINTCLDTNGFVRHYDHIIDEL
HHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHH
LDVTDLVLLDLKELNDQVHQNLIGVPNKRTLEFAKYLQKRNQHTWIRYVVVPGYTDSDHD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCH
VHLLGQFIEGMTNIEKVELLPYHRLGVHKWKTLGLDYELENVLPPTKESLEHIKTILEGY
HHHHHHHHHHHCCHHHHHCCCHHHHCHHHHHHCCCCEEHHHCCCCCHHHHHHHHHHHHHC
GHTVKF
CCCCCC
>Mature Secondary Structure 
SVLGRIHSFESCGTVDGPGIRFILFMQGCLMRCKYCHNRDTWDLEGGKEISVEDLMKEV
CHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
VTYRHFMNATGGGVTASGGEAVLQAEFVRDWFRACKEEGINTCLDTNGFVRHYDHIIDEL
HHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHH
LDVTDLVLLDLKELNDQVHQNLIGVPNKRTLEFAKYLQKRNQHTWIRYVVVPGYTDSDHD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCH
VHLLGQFIEGMTNIEKVELLPYHRLGVHKWKTLGLDYELENVLPPTKESLEHIKTILEGY
HHHHHHHHHHHCCHHHHHCCCHHHHCHHHHHHCCCCEEHHHCCCCCHHHHHHHHHHHHHC
GHTVKF
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800