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The map label for this gene is nqr6 [H]

Identifier: 16272136

GI number: 16272136

Start: 184146

End: 185381

Strand: Direct

Name: nqr6 [H]

Synonym: HI0171

Alternate gene names: 16272136

Gene position: 184146-185381 (Clockwise)

Preceding gene: 16272135

Following gene: 16272137

Centisome position: 10.06

GC content: 39.56

Gene sequence:

>1236_bases
ATGAGCGATTCAGTAATTCTTGCACTCGGTATTGCCGCATTCACGGTTATTGTATTAGTGTTAGTGGCAATCATCTTATT
TGCGAAATCAAAATTAGTCGATTCTGGTGATATTACTATCGACATCAATGACGATCCTGAAAAAGCGATCACATTACCTG
CAGGTGGCAAATTATTAGGGGCTTTAGCAAGTAAAGGTATTTTCGTGTCTTCTGCTTGCGGCGGCGGTGGCTCTTGTGGT
CAATGTATTGTTAAAGTGAAAAATGGTGGCGGAGAAATTCTTCCAACCGAACTTTCTCACATTAACAAACGTGAAGCAAA
AGAGGGATATCGCTTAGCTTGTCAAGTTAATGTAAAAGGTAATATGGAAGTTGAACTTCCAGAAGAAATCTTCGGCGTGA
AAAAATGGGAATGTACTGTTATTTCTAACGATAACAAAGCAACCTTTATCAAAGAGCTTAAATTGGCTATTCCTGAAGGC
GAGGAAGTGCCTTTCCGTGCAGGTGGTTATATCCAAATCGAAGCTGAACCACATGTGGTGAACTATAAAGATTTCGATAT
TCCAGAAGAATACCACGAAGACTGGGATAAATATGATTTATGGCGTTATGTCTCTAAAGTGGACGAGCATATTATCCGTG
CTTACTCAATGGCTTCATACCCTGAAGAGAAAGGTATCATTATGCTTAACGTTCGTATTGCGACTCCACCACCACGCCAA
CCTGATGCGCCTCCGGGTCAAATGTCTTCTTACATTTGGTCATTAAAAGCAGGAGATAAAGTCACAATTTCTGGTCCATT
TGGTGAATTCTTTGCGAAAGAAACTGATGCAGAAATGGTATTTATCGGTGGTGGTGCGGGTATGGCTCCAATGCGTTCAC
ATATCTTCGACCAATTAAAACGTTTACATTCTAAACGTAAAATGTCGTTCTGGTATGGTGCACGTTCTAAACGTGAAATA
TTCTATCAAGAAGACTTTGACCAATTACAAGCTGAAAATCCAAACTTTGTATGGCACGTGGCACTTTCAGATGCATTGCC
TGAAGATAATTGGACTGGCTATACAGGCTTTATTCACAATGTACTTTATGAAAACTACTTGAAAAATCATGAAGCACCAG
AAGACTGTGAATACTATATGTGTGGGCCTCCAGTGATGAATGCAGCTGTAATTAAAATGTTGAAAGATCTTGGTGTTGAA
GATGAAAACATTTTATTAGATGACTTTGGTGGCTGA

Upstream 100 bases:

>100_bases
TGCTGGATTAAAAGGTTTAGGTATTACCTTTATCTCTGTTGGTTTAATGGCACTAGGCTTTATGTCTTTCTCTGGTATTC
AATTATAAGGAGTGTAATCA

Downstream 100 bases:

>100_bases
TTTTAGTTAATCAATTCATCATAAAAGCTGCCCGCACTTTGTGATAAAGTGCGGRCSAAATTTAAGATGTTTTTAAACAT
TTTCTATGATTACAGGTAAG

Product: Na(+)-translocating NADH-quinone reductase subunit F

Products: @TETR01txt*tetrahydropteridine!; NO3-; NAD(P); Proton [C]

Alternate protein names: Na(+)-NQR subunit F; Na(+)-translocating NQR subunit F; NQR complex subunit F; NQR-1 subunit F [H]

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MSDSVILALGIAAFTVIVLVLVAIILFAKSKLVDSGDITIDINDDPEKAITLPAGGKLLGALASKGIFVSSACGGGGSCG
QCIVKVKNGGGEILPTELSHINKREAKEGYRLACQVNVKGNMEVELPEEIFGVKKWECTVISNDNKATFIKELKLAIPEG
EEVPFRAGGYIQIEAEPHVVNYKDFDIPEEYHEDWDKYDLWRYVSKVDEHIIRAYSMASYPEEKGIIMLNVRIATPPPRQ
PDAPPGQMSSYIWSLKAGDKVTISGPFGEFFAKETDAEMVFIGGGAGMAPMRSHIFDQLKRLHSKRKMSFWYGARSKREI
FYQEDFDQLQAENPNFVWHVALSDALPEDNWTGYTGFIHNVLYENYLKNHEAPEDCEYYMCGPPVMNAAVIKMLKDLGVE
DENILLDDFGG

Sequences:

>Translated_411_residues
MSDSVILALGIAAFTVIVLVLVAIILFAKSKLVDSGDITIDINDDPEKAITLPAGGKLLGALASKGIFVSSACGGGGSCG
QCIVKVKNGGGEILPTELSHINKREAKEGYRLACQVNVKGNMEVELPEEIFGVKKWECTVISNDNKATFIKELKLAIPEG
EEVPFRAGGYIQIEAEPHVVNYKDFDIPEEYHEDWDKYDLWRYVSKVDEHIIRAYSMASYPEEKGIIMLNVRIATPPPRQ
PDAPPGQMSSYIWSLKAGDKVTISGPFGEFFAKETDAEMVFIGGGAGMAPMRSHIFDQLKRLHSKRKMSFWYGARSKREI
FYQEDFDQLQAENPNFVWHVALSDALPEDNWTGYTGFIHNVLYENYLKNHEAPEDCEYYMCGPPVMNAAVIKMLKDLGVE
DENILLDDFGG
>Mature_410_residues
SDSVILALGIAAFTVIVLVLVAIILFAKSKLVDSGDITIDINDDPEKAITLPAGGKLLGALASKGIFVSSACGGGGSCGQ
CIVKVKNGGGEILPTELSHINKREAKEGYRLACQVNVKGNMEVELPEEIFGVKKWECTVISNDNKATFIKELKLAIPEGE
EVPFRAGGYIQIEAEPHVVNYKDFDIPEEYHEDWDKYDLWRYVSKVDEHIIRAYSMASYPEEKGIIMLNVRIATPPPRQP
DAPPGQMSSYIWSLKAGDKVTISGPFGEFFAKETDAEMVFIGGGAGMAPMRSHIFDQLKRLHSKRKMSFWYGARSKREIF
YQEDFDQLQAENPNFVWHVALSDALPEDNWTGYTGFIHNVLYENYLKNHEAPEDCEYYMCGPPVMNAAVIKMLKDLGVED
ENILLDDFGG

Specific function: NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na(+) ions from the cytoplasm to the periplasm. The first step is catalyzed by nqrF, which accepts electrons from NADH and reduces

COG id: COG2871

COG function: function code C; Na+-transporting NADH:ubiquinone oxidoreductase, subunit NqrF

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17563926, Length=220, Percent_Identity=28.1818181818182, Blast_Score=71, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6322146, Length=156, Percent_Identity=28.8461538461538, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323510, Length=154, Percent_Identity=27.2727272727273, Blast_Score=73, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6323552, Length=202, Percent_Identity=26.2376237623762, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001709
- InterPro:   IPR010205
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.5.1.34 [C]

Molecular weight: Translated: 45706; Mature: 45574

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSVILALGIAAFTVIVLVLVAIILFAKSKLVDSGDITIDINDDPEKAITLPAGGKLLG
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEECCCCCHHHH
ALASKGIFVSSACGGGGSCGQCIVKVKNGGGEILPTELSHINKREAKEGYRLACQVNVKG
HHHCCCEEEEECCCCCCCCCEEEEEEECCCCCCCCHHHHHCHHHHCCCCEEEEEEEECCC
NMEVELPEEIFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFRAGGYIQIEAEPHVV
CEEEECCHHHHCCCEEEEEEEECCCCCHHEEHHHHCCCCCCCCCCCCCCEEEEECCCCEE
NYKDFDIPEEYHEDWDKYDLWRYVSKVDEHIIRAYSMASYPEEKGIIMLNVRIATPPPRQ
CCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCC
PDAPPGQMSSYIWSLKAGDKVTISGPFGEFFAKETDAEMVFIGGGAGMAPMRSHIFDQLK
CCCCCCHHHHHEEEECCCCEEEEECCCHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHH
RLHSKRKMSFWYGARSKREIFYQEDFDQLQAENPNFVWHVALSDALPEDNWTGYTGFIHN
HHHHHHCCEEEECCCCCCEEEECCCHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHH
VLYENYLKNHEAPEDCEYYMCGPPVMNAAVIKMLKDLGVEDENILLDDFGG
HHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCEEEECCCC
>Mature Secondary Structure 
SDSVILALGIAAFTVIVLVLVAIILFAKSKLVDSGDITIDINDDPEKAITLPAGGKLLG
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEECCCCCHHHH
ALASKGIFVSSACGGGGSCGQCIVKVKNGGGEILPTELSHINKREAKEGYRLACQVNVKG
HHHCCCEEEEECCCCCCCCCEEEEEEECCCCCCCCHHHHHCHHHHCCCCEEEEEEEECCC
NMEVELPEEIFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFRAGGYIQIEAEPHVV
CEEEECCHHHHCCCEEEEEEEECCCCCHHEEHHHHCCCCCCCCCCCCCCEEEEECCCCEE
NYKDFDIPEEYHEDWDKYDLWRYVSKVDEHIIRAYSMASYPEEKGIIMLNVRIATPPPRQ
CCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCC
PDAPPGQMSSYIWSLKAGDKVTISGPFGEFFAKETDAEMVFIGGGAGMAPMRSHIFDQLK
CCCCCCHHHHHEEEECCCCEEEEECCCHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHH
RLHSKRKMSFWYGARSKREIFYQEDFDQLQAENPNFVWHVALSDALPEDNWTGYTGFIHN
HHHHHHCCEEEECCCCCCEEEECCCHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHH
VLYENYLKNHEAPEDCEYYMCGPPVMNAAVIKMLKDLGVEDENILLDDFGG
HHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dihydropteridine; 2 nitric oxide; O2; NAD(P)H [C]

Specific reaction: NAD(P)H + dihydropteridine = NAD(P)+ @TETR01.txt*tetrahydropteridine! 2 nitric oxide + 2 O2 + NAD(P)H = 2 NO3- + NAD(P) + Proton [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA