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The map label for this gene is apbE

Identifier: 16272137

GI number: 16272137

Start: 185525

End: 186565

Strand: Direct

Name: apbE

Synonym: HI0172

Alternate gene names: 16272137

Gene position: 185525-186565 (Clockwise)

Preceding gene: 16272136

Following gene: 16272138

Centisome position: 10.14

GC content: 38.62

Gene sequence:

>1041_bases
ATGAAAAAATTAATAAGCGGTATCATAGCGGTAGCAATGGCATTAAGTCTTGCTGCTTGTCAAAAAGAAACAAAAGTTAT
CTCTTTAAGCGGTAAAACAATGGGGACAACTTATCATGTTAAATACCTTGATGATGGTTCAATAACAGCAACATCTGAAA
AGACGCATGAAGAAATTGAAGCAATCTTAAAAGATGTAAACGCGAAAATGTCCACTTACAAAAAAGATTCGGAATTGAGC
CGCTTCAATCAAAATACCCAAGTGAACACACCGATTGAGATTTCAGCAGATTTTGCCAAAGTATTAGCTGAAGCGATTCG
TTTAAATAAAGTGACCGAAGGCGCGTTGGATGTAACTGTTGGCCCTGTCGTGAATTTATGGGGATTTGGTCCTGAAAAAC
GCCCCGAAAAGCAACCTACACCAGAACAATTAGCTGAACGCCAAGCGTGGGTTGGCATTGATAAAATTACCCTAGATACT
AACAAAGAAAAAGCTACATTAAGTAAAGCACTTCCTCAAGTTTACGTAGATTTATCGTCAATTGCTAAAGGCTTTGGCGT
TGATCAGGTGGCTGAAAAGTTAGAACAATTAAATGCTCAGAATTACATGGTTGAAATCGGCGGTGAAATTCGTGCGAAAG
GAAAAAATATTGAAGGTAAACCTTGGCAGATTGCCATTGAAAAACCAACTACAACAGGCGAAAGAGCGGTTGAAGCGGTC
ATTGGATTAAACAATATGGGAATGGCAAGTTCTGGCGATTACCGTATTTACTTTGAAGAAAATGGCAAACGCTTTGCGCA
CGAAATTGATCCGAAAACAGGTTATCCAATTCAGCATCATTTAGCCTCAATTACGGTACTTGCACCAACCTCAATGACTG
CAGATGGCTTATCTACAGGGTTATTTGTGCTGGGGGAAGACAAGGCGTTAGAAGTGGCTGAGAAAAATAATCTTGCCGTT
TATTTAATCATTAGAACAGATAATGGTTTTGTTACCAAATCATCCTCTGCTTTCAAAAAATTAACAGAAACAAAAGAATA
G

Upstream 100 bases:

>100_bases
ATAAAGTGCGGRCSAAATTTAAGATGTTTTTAAACATTTTCTATGATTACAGGTAAGTTTTGTTGAGTTTGCCAGTAATT
CACCAATTTAGGAAATCCAG

Downstream 100 bases:

>100_bases
TTATGCAAACTTTATTTTTTACTTTAATCGCTTTCGTCGCAATTATATTGTTGATGTCTATCGGATTTATTATCAAAAAA
CAAAGTTTAAAAGGCAGCTG

Product: lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MKKLISGIIAVAMALSLAACQKETKVISLSGKTMGTTYHVKYLDDGSITATSEKTHEEIEAILKDVNAKMSTYKKDSELS
RFNQNTQVNTPIEISADFAKVLAEAIRLNKVTEGALDVTVGPVVNLWGFGPEKRPEKQPTPEQLAERQAWVGIDKITLDT
NKEKATLSKALPQVYVDLSSIAKGFGVDQVAEKLEQLNAQNYMVEIGGEIRAKGKNIEGKPWQIAIEKPTTTGERAVEAV
IGLNNMGMASSGDYRIYFEENGKRFAHEIDPKTGYPIQHHLASITVLAPTSMTADGLSTGLFVLGEDKALEVAEKNNLAV
YLIIRTDNGFVTKSSSAFKKLTETKE

Sequences:

>Translated_346_residues
MKKLISGIIAVAMALSLAACQKETKVISLSGKTMGTTYHVKYLDDGSITATSEKTHEEIEAILKDVNAKMSTYKKDSELS
RFNQNTQVNTPIEISADFAKVLAEAIRLNKVTEGALDVTVGPVVNLWGFGPEKRPEKQPTPEQLAERQAWVGIDKITLDT
NKEKATLSKALPQVYVDLSSIAKGFGVDQVAEKLEQLNAQNYMVEIGGEIRAKGKNIEGKPWQIAIEKPTTTGERAVEAV
IGLNNMGMASSGDYRIYFEENGKRFAHEIDPKTGYPIQHHLASITVLAPTSMTADGLSTGLFVLGEDKALEVAEKNNLAV
YLIIRTDNGFVTKSSSAFKKLTETKE
>Mature_346_residues
MKKLISGIIAVAMALSLAACQKETKVISLSGKTMGTTYHVKYLDDGSITATSEKTHEEIEAILKDVNAKMSTYKKDSELS
RFNQNTQVNTPIEISADFAKVLAEAIRLNKVTEGALDVTVGPVVNLWGFGPEKRPEKQPTPEQLAERQAWVGIDKITLDT
NKEKATLSKALPQVYVDLSSIAKGFGVDQVAEKLEQLNAQNYMVEIGGEIRAKGKNIEGKPWQIAIEKPTTTGERAVEAV
IGLNNMGMASSGDYRIYFEENGKRFAHEIDPKTGYPIQHHLASITVLAPTSMTADGLSTGLFVLGEDKALEVAEKNNLAV
YLIIRTDNGFVTKSSSAFKKLTETKE

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family

Homologues:

Organism=Escherichia coli, GI1788543, Length=339, Percent_Identity=41.5929203539823, Blast_Score=274, Evalue=8e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): APBE_HAEIN (P44550)

Other databases:

- EMBL:   L42023
- PIR:   C64144
- RefSeq:   NP_438340.1
- ProteinModelPortal:   P44550
- SMR:   P44550
- GeneID:   951081
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0172
- TIGR:   HI_0172
- HOGENOM:   HBG292712
- OMA:   GPTMGST
- ProtClustDB:   CLSK870454
- BioCyc:   HINF71421:HI_0172-MONOMER
- InterPro:   IPR003374

Pfam domain/function: PF02424 ApbE

EC number: NA

Molecular weight: Translated: 37781; Mature: 37781

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLISGIIAVAMALSLAACQKETKVISLSGKTMGTTYHVKYLDDGSITATSEKTHEEIE
CHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEECEEEEEEEECCCCEEECCHHHHHHHH
AILKDVNAKMSTYKKDSELSRFNQNTQVNTPIEISADFAKVLAEAIRLNKVTEGALDVTV
HHHHHHCHHHHHHCCHHHHHHHCCCCCCCCCEEECHHHHHHHHHHHHHHHHCCCCEEEEE
GPVVNLWGFGPEKRPEKQPTPEQLAERQAWVGIDKITLDTNKEKATLSKALPQVYVDLSS
CCHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
IAKGFGVDQVAEKLEQLNAQNYMVEIGGEIRAKGKNIEGKPWQIAIEKPTTTGERAVEAV
HHCCCCHHHHHHHHHHCCCCCEEEEECCEEEECCCCCCCCCEEEEEECCCCCHHHHHHHH
IGLNNMGMASSGDYRIYFEENGKRFAHEIDPKTGYPIQHHLASITVLAPTSMTADGLSTG
HCCCCCCCCCCCCEEEEEECCCCEEHHCCCCCCCCCHHHHHEEEEEEECCCCCCCCCCCC
LFVLGEDKALEVAEKNNLAVYLIIRTDNGFVTKSSSAFKKLTETKE
EEEEECCCEEEEECCCCEEEEEEEECCCCEEECCHHHHHHHHHCCC
>Mature Secondary Structure
MKKLISGIIAVAMALSLAACQKETKVISLSGKTMGTTYHVKYLDDGSITATSEKTHEEIE
CHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEECEEEEEEEECCCCEEECCHHHHHHHH
AILKDVNAKMSTYKKDSELSRFNQNTQVNTPIEISADFAKVLAEAIRLNKVTEGALDVTV
HHHHHHCHHHHHHCCHHHHHHHCCCCCCCCCEEECHHHHHHHHHHHHHHHHCCCCEEEEE
GPVVNLWGFGPEKRPEKQPTPEQLAERQAWVGIDKITLDTNKEKATLSKALPQVYVDLSS
CCHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
IAKGFGVDQVAEKLEQLNAQNYMVEIGGEIRAKGKNIEGKPWQIAIEKPTTTGERAVEAV
HHCCCCHHHHHHHHHHCCCCCEEEEECCEEEECCCCCCCCCEEEEEECCCCCHHHHHHHH
IGLNNMGMASSGDYRIYFEENGKRFAHEIDPKTGYPIQHHLASITVLAPTSMTADGLSTG
HCCCCCCCCCCCCEEEEEECCCCEEHHCCCCCCCCCHHHHHEEEEEEECCCCCCCCCCCC
LFVLGEDKALEVAEKNNLAVYLIIRTDNGFVTKSSSAFKKLTETKE
EEEEECCCEEEEECCCCEEEEEEEECCCCEEECCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800