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The map label for this gene is rnhA [H]

Identifier: 16272105

GI number: 16272105

Start: 153323

End: 153787

Strand: Direct

Name: rnhA [H]

Synonym: HI0138

Alternate gene names: 16272105

Gene position: 153323-153787 (Clockwise)

Preceding gene: 16272102

Following gene: 16272106

Centisome position: 8.38

GC content: 36.64

Gene sequence:

>465_bases
ATGCCKAAACAGATTGAAATTTTTACTGATGGATCTTGCTTAGGTAATCCAGGGGCGGGCGGAATTGGTGCCGTATTGCG
TTATAAACAACATGAAAAAACACTCTCCAAAGGCTATTTCCAAACCACCAATAATCGAATGGAATTACGCGCTGTCATTG
AAGCATTAAATACATTAAAAGAACCTTGCTTGATCACGCTTTATAGTGATAGCCAATATATGAAAAATGGCATAACCAAA
TGGATCTTTAACTGGAAAAAAAATAATTGGAAAGCAAGTTCTGGAAAGCCTGTAAAAAACCAAGATTTATGGATAGCCTT
AGATGAATCCATCCAACGTCATAAAATTAATTGGCAATGGGTAAAAGGCCATGCTGGACACAGAGAAAATGAAATTTGCG
ATGAATTAGCAAAAAAAGGGGCAGAAAATCCGACATTGGAAGATATGGGGTACATAGAAGAATAA

Upstream 100 bases:

>100_bases
TTTCAGTATCTAATACAATTTGGCGATTGGGATTAATCATTTGTTCAACCTATCTCTTTCCATTAAAATACTTGCCATTC
TACACAACAACCTTTTTGTT

Downstream 100 bases:

>100_bases
TACAACTGATATAACGTCATATTTTTCGATACCTAAAAATATTTAATACTTAAACCTAAAACAGAATAAAAAATAATCAA
ATTCATTTAAAAAATGTGAT

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MPKQIEIFTDGSCLGNPGAGGIGAVLRYKQHEKTLSKGYFQTTNNRMELRAVIEALNTLKEPCLITLYSDSQYMKNGITK
WIFNWKKNNWKASSGKPVKNQDLWIALDESIQRHKINWQWVKGHAGHRENEICDELAKKGAENPTLEDMGYIEE

Sequences:

>Translated_154_residues
MPKQIEIFTDGSCLGNPGAGGIGAVLRYKQHEKTLSKGYFQTTNNRMELRAVIEALNTLKEPCLITLYSDSQYMKNGITK
WIFNWKKNNWKASSGKPVKNQDLWIALDESIQRHKINWQWVKGHAGHRENEICDELAKKGAENPTLEDMGYIEE
>Mature_153_residues
PKQIEIFTDGSCLGNPGAGGIGAVLRYKQHEKTLSKGYFQTTNNRMELRAVIEALNTLKEPCLITLYSDSQYMKNGITKW
IFNWKKNNWKASSGKPVKNQDLWIALDESIQRHKINWQWVKGHAGHRENEICDELAKKGAENPTLEDMGYIEE

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=144, Percent_Identity=36.8055555555556, Blast_Score=85, Evalue=3e-17,
Organism=Escherichia coli, GI1786408, Length=151, Percent_Identity=65.5629139072848, Blast_Score=213, Evalue=5e-57,
Organism=Caenorhabditis elegans, GI115532542, Length=141, Percent_Identity=35.4609929078014, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17505482, Length=136, Percent_Identity=36.0294117647059, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45552509, Length=144, Percent_Identity=35.4166666666667, Blast_Score=86, Evalue=9e-18,
Organism=Drosophila melanogaster, GI17137476, Length=144, Percent_Identity=35.4166666666667, Blast_Score=86, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 17640; Mature: 17509

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKQIEIFTDGSCLGNPGAGGIGAVLRYKQHEKTLSKGYFQTTNNRMELRAVIEALNTLK
CCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHCC
EPCLITLYSDSQYMKNGITKWIFNWKKNNWKASSGKPVKNQDLWIALDESIQRHKINWQW
CCEEEEEECCCHHHHCCHHHHHEECCCCCEECCCCCCCCCCCEEEEECCCHHHHCCCEEE
VKGHAGHRENEICDELAKKGAENPTLEDMGYIEE
EECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PKQIEIFTDGSCLGNPGAGGIGAVLRYKQHEKTLSKGYFQTTNNRMELRAVIEALNTLK
CCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHCC
EPCLITLYSDSQYMKNGITKWIFNWKKNNWKASSGKPVKNQDLWIALDESIQRHKINWQW
CCEEEEEECCCHHHHCCHHHHHEECCCCCEECCCCCCCCCCCEEEEECCCHHHHCCCEEE
VKGHAGHRENEICDELAKKGAENPTLEDMGYIEE
EECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA