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The map label for this gene is dacA

Identifier: 16272004

GI number: 16272004

Start: 30229

End: 31410

Strand: Reverse

Name: dacA

Synonym: HI0029

Alternate gene names: 16272004

Gene position: 31410-30229 (Counterclockwise)

Preceding gene: 16272005

Following gene: 16272003

Centisome position: 1.72

GC content: 35.36

Gene sequence:

>1182_bases
ATGTTGAAAAGAACAACAAAAATCGCATTTTTATCAAGTTTTGTGGCACTTAGTGCTTTTTCGGTATCTGCAGAGGATAT
GCAGTTTGGCGTAACTCCACCACAAATTACGGCTCAAACGTATGTATTGATGGATTATAATTCAGGTGCAATTTTGACCG
CACTTAATCCAGATCAACGTCAATATCCAGCATCACTGACTAAAATGATGACAAGTTATGTAGTTGGTGTCGCATTAAAA
CAAGGCAAAATCCATAATACCGATATGGTTACCATTGGTGAAAGTGCGTGGGGGCGTAATTTCCCTGATTCATCAAAAAT
GTTTTTAGATTTAAATACACAAGTATCTGTAGCAGACTTAAATCGAGGTGTGATTGTTGTTTCTGGTAATGATGCGACTG
TTGCATTAGCTGAACATATTTCTGGCAATGTACCGAACTTTGTAGAAACAATGAATAAATATGTTCAACAATTTGGTTTG
AAAAATACAAATTTCACCACGCCCCATGGTTTAGACGATCCAAATCAATATTCTTCCGCGCGTGATATGGCAATTATTGG
TGCGCATATTATTCGTGATTTACCTGAAGAATATAAAATTTACTCCGAAAAAAACTTTACATTTAATAAAATCAAACAAG
CTAACCGTAATGGACTATTATGGGATAAAACTATCAATGTTGATGGAATGAAGACAGGGCATACTAGCCAAGCAGGATAT
AACTTAGTCGCTTCAGCGACTACATCAAACAATATGCGTTTAATTTCTGTAGTAATGGGTGTGCCAACTTACAAAGGTCG
TGAAGTAGAAAGTAAGAAACTTTTACAATGGGGTTTTGCTAATTTCGAAACATTTAAAACTTTAGAGGCTGGTAAAGAAA
TTTCAGAACAAAGGGTTTATTATGGCGATAAAAATTCAGTAAAACTTGGTGCTTTAATGGATCACTTTATTACGATCCCT
AAAGGTAAACAAAGTGAGGTTAAAGCTCGTTATGAATTAGCAGATAAAAATTTACAAGCACCATTAGTAAAAGGTCAAGT
GATTGGTAAAGTGGTTTATCAACTGGATGGTAAGGATATTGCAAGTGCCAATTTACAAGTGATGAATGATGTGGGAGAAG
CGGGTATTTTTGGTAAACTATGGGATTGGTTAGTATTGACTGTGAAAGGGTTATTCTCATAA

Upstream 100 bases:

>100_bases
CAAAACAAAAATGGCACAAGTGAGAACTAAATTACAAAAAATGGCAAATAATAAGCCATTAATTGTTTATACGTATAAAA
ATTAATTGTAGGAACACACT

Downstream 100 bases:

>100_bases
AAAGAATTTGAAAATTGACCGCACTTTTTGATTTATTTTATTTTTAGGAAAGAAAATGACAATAGAAAACGATTATGCAA
AACTCAAGGAATTAATGGAA

Product: penicillin-binding protein 5

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MLKRTTKIAFLSSFVALSAFSVSAEDMQFGVTPPQITAQTYVLMDYNSGAILTALNPDQRQYPASLTKMMTSYVVGVALK
QGKIHNTDMVTIGESAWGRNFPDSSKMFLDLNTQVSVADLNRGVIVVSGNDATVALAEHISGNVPNFVETMNKYVQQFGL
KNTNFTTPHGLDDPNQYSSARDMAIIGAHIIRDLPEEYKIYSEKNFTFNKIKQANRNGLLWDKTINVDGMKTGHTSQAGY
NLVASATTSNNMRLISVVMGVPTYKGREVESKKLLQWGFANFETFKTLEAGKEISEQRVYYGDKNSVKLGALMDHFITIP
KGKQSEVKARYELADKNLQAPLVKGQVIGKVVYQLDGKDIASANLQVMNDVGEAGIFGKLWDWLVLTVKGLFS

Sequences:

>Translated_393_residues
MLKRTTKIAFLSSFVALSAFSVSAEDMQFGVTPPQITAQTYVLMDYNSGAILTALNPDQRQYPASLTKMMTSYVVGVALK
QGKIHNTDMVTIGESAWGRNFPDSSKMFLDLNTQVSVADLNRGVIVVSGNDATVALAEHISGNVPNFVETMNKYVQQFGL
KNTNFTTPHGLDDPNQYSSARDMAIIGAHIIRDLPEEYKIYSEKNFTFNKIKQANRNGLLWDKTINVDGMKTGHTSQAGY
NLVASATTSNNMRLISVVMGVPTYKGREVESKKLLQWGFANFETFKTLEAGKEISEQRVYYGDKNSVKLGALMDHFITIP
KGKQSEVKARYELADKNLQAPLVKGQVIGKVVYQLDGKDIASANLQVMNDVGEAGIFGKLWDWLVLTVKGLFS
>Mature_393_residues
MLKRTTKIAFLSSFVALSAFSVSAEDMQFGVTPPQITAQTYVLMDYNSGAILTALNPDQRQYPASLTKMMTSYVVGVALK
QGKIHNTDMVTIGESAWGRNFPDSSKMFLDLNTQVSVADLNRGVIVVSGNDATVALAEHISGNVPNFVETMNKYVQQFGL
KNTNFTTPHGLDDPNQYSSARDMAIIGAHIIRDLPEEYKIYSEKNFTFNKIKQANRNGLLWDKTINVDGMKTGHTSQAGY
NLVASATTSNNMRLISVVMGVPTYKGREVESKKLLQWGFANFETFKTLEAGKEISEQRVYYGDKNSVKLGALMDHFITIP
KGKQSEVKARYELADKNLQAPLVKGQVIGKVVYQLDGKDIASANLQVMNDVGEAGIFGKLWDWLVLTVKGLFS

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space (By similarity)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family

Homologues:

Organism=Escherichia coli, GI1786851, Length=396, Percent_Identity=52.020202020202, Blast_Score=390, Evalue=1e-109,
Organism=Escherichia coli, GI1787062, Length=384, Percent_Identity=51.3020833333333, Blast_Score=377, Evalue=1e-106,
Organism=Escherichia coli, GI87082022, Length=388, Percent_Identity=47.9381443298969, Blast_Score=349, Evalue=2e-97,
Organism=Escherichia coli, GI87082054, Length=256, Percent_Identity=26.5625, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DACA_HAEIN (P44466)

Other databases:

- EMBL:   L42023
- PIR:   I64043
- RefSeq:   NP_438202.1
- PDB:   3A3J
- PDBsum:   3A3J
- ProteinModelPortal:   P44466
- SMR:   P44466
- GeneID:   950922
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0029
- NMPDR:   fig|71421.1.peg.28
- TIGR:   HI_0029
- HOGENOM:   HBG728991
- OMA:   EISEQRV
- ProtClustDB:   CLSK870669
- BioCyc:   HINF71421:HI_0029-MONOMER
- BRENDA:   3.4.16.4
- DrugBank:   DB00303
- GO:   GO:0006508
- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967
- Gene3D:   G3DSA:2.60.410.10
- PRINTS:   PR00725
- SMART:   SM00936

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11; SSF56601 PBP_transp_fold; SSF69189 Peniciliin-bd_prot-assoc

EC number: =3.4.16.4

Molecular weight: Translated: 43414; Mature: 43414

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: NA

Important sites: ACT_SITE 65-65 ACT_SITE 68-68 ACT_SITE 128-128 BINDING 231-231

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRTTKIAFLSSFVALSAFSVSAEDMQFGVTPPQITAQTYVLMDYNSGAILTALNPDQR
CCCCHHHHHHHHHHHHHHHHCCCHHHCEECCCCCCCCEEEEEEEEECCCEEEEEECCCHH
QYPASLTKMMTSYVVGVALKQGKIHNTDMVTIGESAWGRNFPDSSKMFLDLNTQVSVADL
HHHHHHHHHHHHHHHHHHEECCCCCCCCEEEECCHHCCCCCCCCCCEEEEECCEEEEEEC
NRGVIVVSGNDATVALAEHISGNVPNFVETMNKYVQQFGLKNTNFTTPHGLDDPNQYSSA
CCCEEEEECCCCEEEEEHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCC
RDMAIIGAHIIRDLPEEYKIYSEKNFTFNKIKQANRNGLLWDKTINVDGMKTGHTSQAGY
CCHHHHHHHHHHHCCHHHEEECCCCCCHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCC
NLVASATTSNNMRLISVVMGVPTYKGREVESKKLLQWGFANFETFKTLEAGKEISEQRVY
EEEEEECCCCCEEEEEEEECCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCEEE
YGDKNSVKLGALMDHFITIPKGKQSEVKARYELADKNLQAPLVKGQVIGKVVYQLDGKDI
ECCCCCEEHHHHHHHHEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHEEECCCCC
ASANLQVMNDVGEAGIFGKLWDWLVLTVKGLFS
CCCCHHEEHHCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLKRTTKIAFLSSFVALSAFSVSAEDMQFGVTPPQITAQTYVLMDYNSGAILTALNPDQR
CCCCHHHHHHHHHHHHHHHHCCCHHHCEECCCCCCCCEEEEEEEEECCCEEEEEECCCHH
QYPASLTKMMTSYVVGVALKQGKIHNTDMVTIGESAWGRNFPDSSKMFLDLNTQVSVADL
HHHHHHHHHHHHHHHHHHEECCCCCCCCEEEECCHHCCCCCCCCCCEEEEECCEEEEEEC
NRGVIVVSGNDATVALAEHISGNVPNFVETMNKYVQQFGLKNTNFTTPHGLDDPNQYSSA
CCCEEEEECCCCEEEEEHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCC
RDMAIIGAHIIRDLPEEYKIYSEKNFTFNKIKQANRNGLLWDKTINVDGMKTGHTSQAGY
CCHHHHHHHHHHHCCHHHEEECCCCCCHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCC
NLVASATTSNNMRLISVVMGVPTYKGREVESKKLLQWGFANFETFKTLEAGKEISEQRVY
EEEEEECCCCCEEEEEEEECCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCEEE
YGDKNSVKLGALMDHFITIPKGKQSEVKARYELADKNLQAPLVKGQVIGKVVYQLDGKDI
ECCCCCEEHHHHHHHHEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHEEECCCCC
ASANLQVMNDVGEAGIFGKLWDWLVLTVKGLFS
CCCCHHEEHHCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800