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The map label for this gene is gapdH

Identifier: 16271977

GI number: 16271977

Start: 2

End: 1021

Strand: Direct

Name: gapdH

Synonym: HI0001

Alternate gene names: 16271977

Gene position: 2-1021 (Clockwise)

Preceding gene: NA

Following gene: 16271978

Centisome position: 0.0

GC content: 40.78

Gene sequence:

>1020_bases
ATGGCAATTAAAATTGGTATCAATGGTTTTGGTCGTATCGGCCGTATCGTATTCCGTGCAGCACAACACCGTGATGACAT
TGAAGTTGTAGGTATTAACGACTTAATCGACGTTGAATACATGGCTTATATGTTGAAATATGATTCAACTCACGGTCGTT
TCGACGGCACTGTTGAAGTGAAAGATGGTAACTTAGTGGTTAATGGTAAAACTATCCGTGTAACTGCAGAACGTGATCCA
GCAAACTTAAACTGGGGTGCAATCGGTGTTGATATCGCTGTTGAAGCGACTGGTTTATTCTTAACTGATGAAACTGCTCG
TAAACATATCACTGCAGGCGCAAAAAAAGTTGTATTAACTGGCCCATCTAAAGATGCAACCCCTATGTTCGTTCGTGGTG
TAAACTTCAACGCATACGCAGGTCAAGATATCGTTTCTAACGCATCTTGTACAACAAACTGTTTAGCTCCTTTAGCACGT
GTTGTTCATGAAACTTTCGGTATCAAAGATGGTTTAATGACCACTGTTCACGCAACGACTGCAACTCAAAAAACTGTGGA
TGGTCCATCAGCTAAAGACTGGCGCGGCGGCCGCGGTGCATCACAAAACATCATTCCATCTTCAACAGGTGCAGCGAAAG
CAGTAGGTAAAGTATTACCTGCATTAAACGGTAAATTAACTGGTATGGCTTTCCGTGTTCCAACGCCAAACGTATCTGTT
GTTGATTTAACAGTTAATCTTGAAAAACCAGCTTCTTATGATGCAATCAAACAAGCAATCAAAGATGCAGCGGAAGGTAA
AACGTTCAATGGCGAATTAAAAGGCGTATTAGGTTACACTGAAGATGCTGTTGTTTCTACTGACTTCAACGGTTGTGCTT
TAACTTCTGTATTTGATGCAGACGCTGGTATCGCATTAACTGATTCTTTCGTTAAATTGGTATCTTGGTACGATAACGAA
ACGGGTTACTCAAACAAAGTATTAGACTTAGTAGCTCATATCTACAACTACAAAGGCTAA

Upstream 100 bases:

>100_bases
TAAAAATGCTCGTGCTATACTCTGTGCGTTGTCTTACTGAGTGAGCAGTATTACTCAAAGCAAACAGATTTGTTTAACTT
AAATAAAAGGTGAAAATCTT

Downstream 100 bases:

>100_bases
TTAAAACTTTGAAAAAATTAACCGCTCTTCGGAGCGTTTTTTATTATCTAGAATTTAATTTACGCTCTAAAAATGAACAA
GGGATCACTAAAAATAATTT

Product: glyceraldehyde-3-phosphate dehydrogenase

Products: NA

Alternate protein names: GAPDH

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MAIKIGINGFGRIGRIVFRAAQHRDDIEVVGINDLIDVEYMAYMLKYDSTHGRFDGTVEVKDGNLVVNGKTIRVTAERDP
ANLNWGAIGVDIAVEATGLFLTDETARKHITAGAKKVVLTGPSKDATPMFVRGVNFNAYAGQDIVSNASCTTNCLAPLAR
VVHETFGIKDGLMTTVHATTATQKTVDGPSAKDWRGGRGASQNIIPSSTGAAKAVGKVLPALNGKLTGMAFRVPTPNVSV
VDLTVNLEKPASYDAIKQAIKDAAEGKTFNGELKGVLGYTEDAVVSTDFNGCALTSVFDADAGIALTDSFVKLVSWYDNE
TGYSNKVLDLVAHIYNYKG

Sequences:

>Translated_339_residues
MAIKIGINGFGRIGRIVFRAAQHRDDIEVVGINDLIDVEYMAYMLKYDSTHGRFDGTVEVKDGNLVVNGKTIRVTAERDP
ANLNWGAIGVDIAVEATGLFLTDETARKHITAGAKKVVLTGPSKDATPMFVRGVNFNAYAGQDIVSNASCTTNCLAPLAR
VVHETFGIKDGLMTTVHATTATQKTVDGPSAKDWRGGRGASQNIIPSSTGAAKAVGKVLPALNGKLTGMAFRVPTPNVSV
VDLTVNLEKPASYDAIKQAIKDAAEGKTFNGELKGVLGYTEDAVVSTDFNGCALTSVFDADAGIALTDSFVKLVSWYDNE
TGYSNKVLDLVAHIYNYKG
>Mature_338_residues
AIKIGINGFGRIGRIVFRAAQHRDDIEVVGINDLIDVEYMAYMLKYDSTHGRFDGTVEVKDGNLVVNGKTIRVTAERDPA
NLNWGAIGVDIAVEATGLFLTDETARKHITAGAKKVVLTGPSKDATPMFVRGVNFNAYAGQDIVSNASCTTNCLAPLARV
VHETFGIKDGLMTTVHATTATQKTVDGPSAKDWRGGRGASQNIIPSSTGAAKAVGKVLPALNGKLTGMAFRVPTPNVSVV
DLTVNLEKPASYDAIKQAIKDAAEGKTFNGELKGVLGYTEDAVVSTDFNGCALTSVFDADAGIALTDSFVKLVSWYDNET
GYSNKVLDLVAHIYNYKG

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family

Homologues:

Organism=Homo sapiens, GI7669492, Length=334, Percent_Identity=63.7724550898204, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI7657116, Length=337, Percent_Identity=61.1275964391691, Blast_Score=416, Evalue=1e-116,
Organism=Escherichia coli, GI1788079, Length=334, Percent_Identity=83.2335329341317, Blast_Score=571, Evalue=1e-164,
Organism=Escherichia coli, GI1789295, Length=334, Percent_Identity=38.9221556886228, Blast_Score=237, Evalue=8e-64,
Organism=Caenorhabditis elegans, GI32566163, Length=338, Percent_Identity=63.0177514792899, Blast_Score=412, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17568413, Length=338, Percent_Identity=63.0177514792899, Blast_Score=412, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17534677, Length=339, Percent_Identity=61.3569321533923, Blast_Score=408, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17534679, Length=339, Percent_Identity=60.7669616519174, Blast_Score=405, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6322409, Length=334, Percent_Identity=65.8682634730539, Blast_Score=436, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6321631, Length=334, Percent_Identity=65.8682634730539, Blast_Score=431, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6322468, Length=334, Percent_Identity=64.9700598802395, Blast_Score=429, Evalue=1e-121,
Organism=Drosophila melanogaster, GI17933600, Length=333, Percent_Identity=65.7657657657658, Blast_Score=429, Evalue=1e-120,
Organism=Drosophila melanogaster, GI18110149, Length=333, Percent_Identity=65.7657657657658, Blast_Score=429, Evalue=1e-120,
Organism=Drosophila melanogaster, GI85725000, Length=333, Percent_Identity=64.5645645645646, Blast_Score=424, Evalue=1e-119,
Organism=Drosophila melanogaster, GI22023983, Length=333, Percent_Identity=64.5645645645646, Blast_Score=424, Evalue=1e-119,
Organism=Drosophila melanogaster, GI19922412, Length=331, Percent_Identity=61.9335347432024, Blast_Score=407, Evalue=1e-114,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): G3P_HAEIN (P44304)

Other databases:

- EMBL:   L42023
- PIR:   G64041
- RefSeq:   NP_438174.1
- ProteinModelPortal:   P44304
- SMR:   P44304
- GeneID:   950899
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0001
- NMPDR:   fig|71421.1.peg.1
- TIGR:   HI_0001
- HOGENOM:   HBG571736
- OMA:   DLKWDAI
- ProtClustDB:   CLSK870299
- BioCyc:   HINF71421:HI_0001-MONOMER
- BRENDA:   1.2.1.12
- GO:   GO:0005737
- GO:   GO:0006096
- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10836
- PIRSF:   PIRSF000149
- PRINTS:   PR00078
- SMART:   SM00846
- TIGRFAMs:   TIGR01534

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N

EC number: =1.2.1.12

Molecular weight: Translated: 36052; Mature: 35921

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00071 GAPDH

Important sites: ACT_SITE 150-150 BINDING 34-34 BINDING 78-78 BINDING 180-180 BINDING 232-232 BINDING 319-319

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIKIGINGFGRIGRIVFRAAQHRDDIEVVGINDLIDVEYMAYMLKYDSTHGRFDGTVEV
CEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCEEEE
KDGNLVVNGKTIRVTAERDPANLNWGAIGVDIAVEATGLFLTDETARKHITAGAKKVVLT
ECCCEEECCEEEEEEECCCCCCCCCCEEEEEEEEEEEEEEEECHHHHHHHCCCCCEEEEE
GPSKDATPMFVRGVNFNAYAGQDIVSNASCTTNCLAPLARVVHETFGIKDGLMTTVHATT
CCCCCCCCEEEEECCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
ATQKTVDGPSAKDWRGGRGASQNIIPSSTGAAKAVGKVLPALNGKLTGMAFRVPTPNVSV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCEE
VDLTVNLEKPASYDAIKQAIKDAAEGKTFNGELKGVLGYTEDAVVSTDFNGCALTSVFDA
EEEEEECCCCCCHHHHHHHHHHHHCCCEECCCEEEEECCCCCEEEEECCCCEEEEEEECC
DAGIALTDSFVKLVSWYDNETGYSNKVLDLVAHIYNYKG
CCCEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AIKIGINGFGRIGRIVFRAAQHRDDIEVVGINDLIDVEYMAYMLKYDSTHGRFDGTVEV
EEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCEEEE
KDGNLVVNGKTIRVTAERDPANLNWGAIGVDIAVEATGLFLTDETARKHITAGAKKVVLT
ECCCEEECCEEEEEEECCCCCCCCCCEEEEEEEEEEEEEEEECHHHHHHHCCCCCEEEEE
GPSKDATPMFVRGVNFNAYAGQDIVSNASCTTNCLAPLARVVHETFGIKDGLMTTVHATT
CCCCCCCCEEEEECCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
ATQKTVDGPSAKDWRGGRGASQNIIPSSTGAAKAVGKVLPALNGKLTGMAFRVPTPNVSV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCEE
VDLTVNLEKPASYDAIKQAIKDAAEGKTFNGELKGVLGYTEDAVVSTDFNGCALTSVFDA
EEEEEECCCCCCHHHHHHHHHHHHCCCEECCCEEEEECCCCCEEEEECCCCEEEEEEECC
DAGIALTDSFVKLVSWYDNETGYSNKVLDLVAHIYNYKG
CCCEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800