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The map label for this gene is bkdA2 [H]

Identifier: 99082617

GI number: 99082617

Start: 2935882

End: 2936895

Strand: Reverse

Name: bkdA2 [H]

Synonym: TM1040_2777

Alternate gene names: 99082617

Gene position: 2936895-2935882 (Counterclockwise)

Preceding gene: 99082618

Following gene: 99082616

Centisome position: 91.75

GC content: 59.96

Gene sequence:

>1014_bases
ATGGCCCAGATGACCATGATTGAGGCCCTGCGCGACGCGCATGATGTGGCCATGGAGAAAGACGACCGTGTTGTCGTCTA
CGGTGAGGATGTCGGGTATTTTGGCGGTGTGTTTCGTGTCACTGCAGGCCTGCAGGCGAAATACGGCAAGTCACGCTGCT
TTGATACGCCTATCAACGAGGCGGGTATTGTTGGCACCGCGATCGGGATGGCCGCCTATGGTCTGCGCCCTGTGATCGAA
ATCCAGTTCGCCGATTACGTCTATCCGGCCATTGACCAGATCGTCTCAGAGGCCGCCCGCCTGCGGCATCGCTCGGCGGG
GGATTTCACCTGCCCGATCGTGATCCGCATGCCCACCGGTGGGGGCATCTTTGGCGGGCAGACCCACAGCCAGTCGCCCG
AGGCATTCTTTACCCATTCCTCTGGTCTGAAAACGGTAGTGCCTTCCAATCCAAGCGATGCCAAGGGGCTGCTCCTCGCT
GCGATCGAAGACCCGGATCCGGTGATCTTTCTGGAGCCAAAGCGGCTCTATAATGGGCCTTTTGACGGATATCACGACCG
TCCCGTTACCAGTTGGAAGAAGCACCCACTGGGTGATGTCGCAGACGGCTACAACCCGATCCCGCTTGGCAAGGCCGCCA
TCCGTCGCAAAGGCAACGACGTCACGGTGCTGGCCTATGGCACCATGGTCTATGTCGCCGAAGCCGCCGCCGAAGAAAGC
GGCGTCGACGCCGAGGTGATCGACCTGCGCACTCTGCTGCCCCTGGACCTCGAGACCATCGTGGCCTCTGTGGAAAAGAC
CGGGCGCTGCGTCATCGTGCATGAGGCGACCAAGACCTCTGGCTTTGGCGCGGAACTGATGTCGATTGTGCAGGAAAACT
GTTTCTACAGCCTCGAAGCGCCCATCATTCGCGTTACTGGCTGGGATACGCCCTACCCGCATGCGCAGGAATGGAATTAC
TTCCCCGGCCCAGAACGGGTGGGCGAAGCGCTGAAGAAATGCATGGAGGACTGA

Upstream 100 bases:

>100_bases
GGTGGTGGCAAGGGCCCCTCGCCGCGCGACATGTTCGAGGGCGTGTTCGAGAAAATGCCGCCTCATCTGATTAGGCAACG
ACAGGAAGCGGGATACTGAC

Downstream 100 bases:

>100_bases
GCGGATGGGAATCTTTGCAATTCGCTTGCCCGACGTAGGTGAAGGTATCGCTGAGGCCGAGCTTACGGAGTGGCATGTAA
AGCCGGGTGACATCGTCAAG

Product: branched-chain alpha-keto acid dehydrogenase E1 component

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component beta chain; BCKDH E1-beta [H]

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MAQMTMIEALRDAHDVAMEKDDRVVVYGEDVGYFGGVFRVTAGLQAKYGKSRCFDTPINEAGIVGTAIGMAAYGLRPVIE
IQFADYVYPAIDQIVSEAARLRHRSAGDFTCPIVIRMPTGGGIFGGQTHSQSPEAFFTHSSGLKTVVPSNPSDAKGLLLA
AIEDPDPVIFLEPKRLYNGPFDGYHDRPVTSWKKHPLGDVADGYNPIPLGKAAIRRKGNDVTVLAYGTMVYVAEAAAEES
GVDAEVIDLRTLLPLDLETIVASVEKTGRCVIVHEATKTSGFGAELMSIVQENCFYSLEAPIIRVTGWDTPYPHAQEWNY
FPGPERVGEALKKCMED

Sequences:

>Translated_337_residues
MAQMTMIEALRDAHDVAMEKDDRVVVYGEDVGYFGGVFRVTAGLQAKYGKSRCFDTPINEAGIVGTAIGMAAYGLRPVIE
IQFADYVYPAIDQIVSEAARLRHRSAGDFTCPIVIRMPTGGGIFGGQTHSQSPEAFFTHSSGLKTVVPSNPSDAKGLLLA
AIEDPDPVIFLEPKRLYNGPFDGYHDRPVTSWKKHPLGDVADGYNPIPLGKAAIRRKGNDVTVLAYGTMVYVAEAAAEES
GVDAEVIDLRTLLPLDLETIVASVEKTGRCVIVHEATKTSGFGAELMSIVQENCFYSLEAPIIRVTGWDTPYPHAQEWNY
FPGPERVGEALKKCMED
>Mature_336_residues
AQMTMIEALRDAHDVAMEKDDRVVVYGEDVGYFGGVFRVTAGLQAKYGKSRCFDTPINEAGIVGTAIGMAAYGLRPVIEI
QFADYVYPAIDQIVSEAARLRHRSAGDFTCPIVIRMPTGGGIFGGQTHSQSPEAFFTHSSGLKTVVPSNPSDAKGLLLAA
IEDPDPVIFLEPKRLYNGPFDGYHDRPVTSWKKHPLGDVADGYNPIPLGKAAIRRKGNDVTVLAYGTMVYVAEAAAEESG
VDAEVIDLRTLLPLDLETIVASVEKTGRCVIVHEATKTSGFGAELMSIVQENCFYSLEAPIIRVTGWDTPYPHAQEWNYF
PGPERVGEALKKCMED

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4557353, Length=336, Percent_Identity=49.1071428571429, Blast_Score=302, Evalue=3e-82,
Organism=Homo sapiens, GI34101272, Length=336, Percent_Identity=49.1071428571429, Blast_Score=302, Evalue=3e-82,
Organism=Homo sapiens, GI156564403, Length=340, Percent_Identity=39.1176470588235, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI291084858, Length=339, Percent_Identity=37.4631268436578, Blast_Score=197, Evalue=8e-51,
Organism=Caenorhabditis elegans, GI17506935, Length=339, Percent_Identity=47.787610619469, Blast_Score=288, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI17538422, Length=343, Percent_Identity=40.5247813411079, Blast_Score=229, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6319698, Length=327, Percent_Identity=37.6146788990826, Blast_Score=212, Evalue=8e-56,
Organism=Drosophila melanogaster, GI160714828, Length=335, Percent_Identity=47.1641791044776, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI160714832, Length=335, Percent_Identity=47.1641791044776, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI21358145, Length=340, Percent_Identity=40, Blast_Score=232, Evalue=3e-61,
Organism=Drosophila melanogaster, GI24650940, Length=340, Percent_Identity=40, Blast_Score=232, Evalue=3e-61,
Organism=Drosophila melanogaster, GI24650943, Length=90, Percent_Identity=45.5555555555556, Blast_Score=85, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24650945, Length=90, Percent_Identity=45.5555555555556, Blast_Score=85, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 36676; Mature: 36545

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQMTMIEALRDAHDVAMEKDDRVVVYGEDVGYFGGVFRVTAGLQAKYGKSRCFDTPINE
CCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHCCHHHEECCCHHCCCCCCCCCCCCCC
AGIVGTAIGMAAYGLRPVIEIQFADYVYPAIDQIVSEAARLRHRSAGDFTCPIVIRMPTG
CCCHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCC
GGIFGGQTHSQSPEAFFTHSSGLKTVVPSNPSDAKGLLLAAIEDPDPVIFLEPKRLYNGP
CCCCCCCCCCCCCCEEEEECCCCEEECCCCCCCCCCEEEEEECCCCCEEEECCHHHHCCC
FDGYHDRPVTSWKKHPLGDVADGYNPIPLGKAAIRRKGNDVTVLAYGTMVYVAEAAAEES
CCCCCCCCCCCCCCCCCCCHHCCCCCCCCCHHHHHCCCCCEEEEEECCEEEHHHHHHHHC
GVDAEVIDLRTLLPLDLETIVASVEKTGRCVIVHEATKTSGFGAELMSIVQENCFYSLEA
CCCEEEEHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHCEEEECC
PIIRVTGWDTPYPHAQEWNYFPGPERVGEALKKCMED
CEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
AQMTMIEALRDAHDVAMEKDDRVVVYGEDVGYFGGVFRVTAGLQAKYGKSRCFDTPINE
CHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHCCHHHEECCCHHCCCCCCCCCCCCCC
AGIVGTAIGMAAYGLRPVIEIQFADYVYPAIDQIVSEAARLRHRSAGDFTCPIVIRMPTG
CCCHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCC
GGIFGGQTHSQSPEAFFTHSSGLKTVVPSNPSDAKGLLLAAIEDPDPVIFLEPKRLYNGP
CCCCCCCCCCCCCCEEEEECCCCEEECCCCCCCCCCEEEEEECCCCCEEEECCHHHHCCC
FDGYHDRPVTSWKKHPLGDVADGYNPIPLGKAAIRRKGNDVTVLAYGTMVYVAEAAAEES
CCCCCCCCCCCCCCCCCCCHHCCCCCCCCCHHHHHCCCCCEEEEEECCEEEHHHHHHHHC
GVDAEVIDLRTLLPLDLETIVASVEKTGRCVIVHEATKTSGFGAELMSIVQENCFYSLEA
CCCEEEEHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHCEEEECC
PIIRVTGWDTPYPHAQEWNYFPGPERVGEALKKCMED
CEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3416875; 10426958 [H]