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The map label for this gene is bkdA1 [H]

Identifier: 99082618

GI number: 99082618

Start: 2936897

End: 2938138

Strand: Reverse

Name: bkdA1 [H]

Synonym: TM1040_2778

Alternate gene names: 99082618

Gene position: 2938138-2936897 (Counterclockwise)

Preceding gene: 99082619

Following gene: 99082617

Centisome position: 91.79

GC content: 61.35

Gene sequence:

>1242_bases
ATGAGCGACAACGAAACCTATGCACCGCTCGCACTGAATGTCCCGGAGCCGGGCTGCCGTCCGGGCGATACGCCTGATTT
TTCGTATTTCGAGGTGCCCCGCGCCGGAGCCGTGGCGCGGCCTTCGGTCGATGTGGACCCTGACGACATGCGTGAGATGG
CGTTTTCCATCGTGCGTGTCCTCAACAAAGAGGGCGAAGCGGTGGGCGACTGGGCCGGAACGCTCTCTCCGGAAGAGTTG
CGCGAAGGATTGCGGGACATGATGCTTCTGCGCGCCTTTGATGCGCGGATGCTGAACGCGCAGCGCCAGGGCAAGACCAG
TTTCTACATGCAGCATCTTGGCGAGGAGGCCGTCAGCTGTGCCTTCTCTCGCGCGCTCAAGGATGGGGATATGAATTTTC
CCACCTATCGGCAGGCGGGCCTGTTGATTGCGCGGGACTATCCGCTGGTCACCATGATGAACCAGATCTATTCCAACGCC
GACGACCCGTTGCACGGGCGGCAGTTGCCGATCATGTACTCCTCAAAAGAACACGGCTTCTTTTCGATTTCGGGCAACCT
CGGCACCCAGTTCGTGCAGGCTGTCGGCTGGGCGATGGCCTCAGCGATTTCCGGTGATACCAAGATCGCCGCAGGCTGGA
TTGGCGATGGTTCCACCGCCGAGAGCGACTTTCACGCCGCGATGGTGTTTGCCTCGACTTATAATGCGCCGGTGGTGCTC
AATATCGTCAACAACCAGTGGGCGATCTCGACCTTTCAGGGCATCGCACGCGGTGGGGTGGGCACCTTTGCTGCGCGCGG
GCATGGCTTTGGCATCGCGTCGATCCGGGTAGATGGAAACGACTATCTCGCGGTCAACGCGGTGGCCAAATGGGCGGCCG
AACGCGCACGTCTGGGCCTTGGCCCCACCTTGATCGAGCATGTGACCTATCGCGCGGGTGGCCATTCCACCAGCGATGAC
CCTTCGGCCTATCGCTCCGCAGATGAGGGCGCGGCATGGCCGCTTGGTGATCCCATCGACCGTCTCAAACGCCACCTTAT
CCGGATCGGCGAGTGGAGTGAGGAGCGCCACAGTCAGGCTGAGGCGGAGTTGATGGATCAGGTCATCACCGCCCAGAAAG
AGGCCGAAAAGGTCGGCACGCTCGGTGGTGGCAAGGGCCCCTCGCCGCGCGACATGTTCGAGGGCGTGTTCGAGAAAATG
CCGCCTCATCTGATTAGGCAACGACAGGAAGCGGGATACTGA

Upstream 100 bases:

>100_bases
TTGACCTCAGCGACAAGACCGGGCGCACCACCACCGCCGTGGCAGACGACATGGTTGCAGATATTCTCGCCAAGGCCTCG
AACACCGCGCGAGGTGCAGC

Downstream 100 bases:

>100_bases
CATGGCCCAGATGACCATGATTGAGGCCCTGCGCGACGCGCATGATGTGGCCATGGAGAAAGACGACCGTGTTGTCGTCT
ACGGTGAGGATGTCGGGTAT

Product: branched-chain alpha-keto acid dehydrogenase E1 component

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; BCKDH E1-alpha [H]

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MSDNETYAPLALNVPEPGCRPGDTPDFSYFEVPRAGAVARPSVDVDPDDMREMAFSIVRVLNKEGEAVGDWAGTLSPEEL
REGLRDMMLLRAFDARMLNAQRQGKTSFYMQHLGEEAVSCAFSRALKDGDMNFPTYRQAGLLIARDYPLVTMMNQIYSNA
DDPLHGRQLPIMYSSKEHGFFSISGNLGTQFVQAVGWAMASAISGDTKIAAGWIGDGSTAESDFHAAMVFASTYNAPVVL
NIVNNQWAISTFQGIARGGVGTFAARGHGFGIASIRVDGNDYLAVNAVAKWAAERARLGLGPTLIEHVTYRAGGHSTSDD
PSAYRSADEGAAWPLGDPIDRLKRHLIRIGEWSEERHSQAEAELMDQVITAQKEAEKVGTLGGGKGPSPRDMFEGVFEKM
PPHLIRQRQEAGY

Sequences:

>Translated_413_residues
MSDNETYAPLALNVPEPGCRPGDTPDFSYFEVPRAGAVARPSVDVDPDDMREMAFSIVRVLNKEGEAVGDWAGTLSPEEL
REGLRDMMLLRAFDARMLNAQRQGKTSFYMQHLGEEAVSCAFSRALKDGDMNFPTYRQAGLLIARDYPLVTMMNQIYSNA
DDPLHGRQLPIMYSSKEHGFFSISGNLGTQFVQAVGWAMASAISGDTKIAAGWIGDGSTAESDFHAAMVFASTYNAPVVL
NIVNNQWAISTFQGIARGGVGTFAARGHGFGIASIRVDGNDYLAVNAVAKWAAERARLGLGPTLIEHVTYRAGGHSTSDD
PSAYRSADEGAAWPLGDPIDRLKRHLIRIGEWSEERHSQAEAELMDQVITAQKEAEKVGTLGGGKGPSPRDMFEGVFEKM
PPHLIRQRQEAGY
>Mature_412_residues
SDNETYAPLALNVPEPGCRPGDTPDFSYFEVPRAGAVARPSVDVDPDDMREMAFSIVRVLNKEGEAVGDWAGTLSPEELR
EGLRDMMLLRAFDARMLNAQRQGKTSFYMQHLGEEAVSCAFSRALKDGDMNFPTYRQAGLLIARDYPLVTMMNQIYSNAD
DPLHGRQLPIMYSSKEHGFFSISGNLGTQFVQAVGWAMASAISGDTKIAAGWIGDGSTAESDFHAAMVFASTYNAPVVLN
IVNNQWAISTFQGIARGGVGTFAARGHGFGIASIRVDGNDYLAVNAVAKWAAERARLGLGPTLIEHVTYRAGGHSTSDDP
SAYRSADEGAAWPLGDPIDRLKRHLIRIGEWSEERHSQAEAELMDQVITAQKEAEKVGTLGGGKGPSPRDMFEGVFEKMP
PHLIRQRQEAGY

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCKDHA family [H]

Homologues:

Organism=Homo sapiens, GI11386135, Length=355, Percent_Identity=40, Blast_Score=238, Evalue=6e-63,
Organism=Homo sapiens, GI258645172, Length=355, Percent_Identity=39.7183098591549, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI4505685, Length=256, Percent_Identity=25.390625, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI291084742, Length=256, Percent_Identity=25.390625, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI291084744, Length=261, Percent_Identity=24.904214559387, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI4885543, Length=292, Percent_Identity=25.3424657534247, Blast_Score=82, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI86563355, Length=360, Percent_Identity=40.2777777777778, Blast_Score=235, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI86563357, Length=360, Percent_Identity=40.2777777777778, Blast_Score=234, Evalue=5e-62,
Organism=Caenorhabditis elegans, GI17536047, Length=343, Percent_Identity=25.9475218658892, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI32564172, Length=343, Percent_Identity=25.9475218658892, Blast_Score=98, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6321026, Length=297, Percent_Identity=28.2828282828283, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21355903, Length=391, Percent_Identity=36.0613810741688, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24639748, Length=305, Percent_Identity=26.5573770491803, Blast_Score=85, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017
- InterPro:   IPR022593 [H]

Pfam domain/function: PF00676 E1_dh; PF12573 OxoDH_E1alpha_N [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 45009; Mature: 44878

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNETYAPLALNVPEPGCRPGDTPDFSYFEVPRAGAVARPSVDVDPDDMREMAFSIVRV
CCCCCCCCCEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LNKEGEAVGDWAGTLSPEELREGLRDMMLLRAFDARMLNAQRQGKTSFYMQHLGEEAVSC
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
AFSRALKDGDMNFPTYRQAGLLIARDYPLVTMMNQIYSNADDPLHGRQLPIMYSSKEHGF
HHHHHHHCCCCCCCCHHHCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCE
FSISGNLGTQFVQAVGWAMASAISGDTKIAAGWIGDGSTAESDFHAAMVFASTYNAPVVL
EEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHEEEEEEECCCCCEEE
NIVNNQWAISTFQGIARGGVGTFAARGHGFGIASIRVDGNDYLAVNAVAKWAAERARLGL
EEECCCCHHHHHHHHHHCCCCHHEECCCCCEEEEEEECCCCEEEHHHHHHHHHHHHHCCC
GPTLIEHVTYRAGGHSTSDDPSAYRSADEGAAWPLGDPIDRLKRHLIRIGEWSEERHSQA
CHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
EAELMDQVITAQKEAEKVGTLGGGKGPSPRDMFEGVFEKMPPHLIRQRQEAGY
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCC
>Mature Secondary Structure 
SDNETYAPLALNVPEPGCRPGDTPDFSYFEVPRAGAVARPSVDVDPDDMREMAFSIVRV
CCCCCCCCEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LNKEGEAVGDWAGTLSPEELREGLRDMMLLRAFDARMLNAQRQGKTSFYMQHLGEEAVSC
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
AFSRALKDGDMNFPTYRQAGLLIARDYPLVTMMNQIYSNADDPLHGRQLPIMYSSKEHGF
HHHHHHHCCCCCCCCHHHCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCE
FSISGNLGTQFVQAVGWAMASAISGDTKIAAGWIGDGSTAESDFHAAMVFASTYNAPVVL
EEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHEEEEEEECCCCCEEE
NIVNNQWAISTFQGIARGGVGTFAARGHGFGIASIRVDGNDYLAVNAVAKWAAERARLGL
EEECCCCHHHHHHHHHHCCCCHHEECCCCCEEEEEEECCCCEEEHHHHHHHHHHHHHCCC
GPTLIEHVTYRAGGHSTSDDPSAYRSADEGAAWPLGDPIDRLKRHLIRIGEWSEERHSQA
CHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
EAELMDQVITAQKEAEKVGTLGGGKGPSPRDMFEGVFEKMPPHLIRQRQEAGY
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]