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The map label for this gene is bkdB [H]

Identifier: 99082616

GI number: 99082616

Start: 2934612

End: 2935877

Strand: Reverse

Name: bkdB [H]

Synonym: TM1040_2776

Alternate gene names: 99082616

Gene position: 2935877-2934612 (Counterclockwise)

Preceding gene: 99082617

Following gene: 99082615

Centisome position: 91.72

GC content: 61.22

Gene sequence:

>1266_bases
ATGGGAATCTTTGCAATTCGCTTGCCCGACGTAGGTGAAGGTATCGCTGAGGCCGAGCTTACGGAGTGGCATGTAAAGCC
GGGTGACATCGTCAAGGAAGACGATGTGCTCGCAGCTGTCATGACCGACAAGGCTGCCGTCGAGGTGCCTTCCTCGGTAG
AGGGCAAGGTGGTCGAACTCGGGGGAGAGATCGGCGATATGCTCGCGATCGGCTGCGTGCTGGTACGAATCGAAGTGGAT
GGTGAAGGCAATGAGGCAGCCTCTGTCGCTGAACCCGAACCAACCCCGGCCCCCAAAGAGCAGAAGGCGGAGCCGGAGCC
AGAACGAGGGGACAAGCCGGCAGAGCCCGCGCGACCCGTGGTGAAAACCGGAAAACCCGTCGCGCGCGCAAAGAACACCA
AACCTCTGGCGGCACCCTCGGTCCGCGCACGGGCGCGCGAGGAAGGTGTGGATCTGCGTCAAGTGCCCGGGAGCGGCCCG
GGTGGGCGTATCAGCCACGGGGATCTCGAGAACTGGATCGCCTCTGGCGGCATCCAACAAGGCAGCGTCAAGCGCGGCAA
GAACACGGGCGTCGAGGATGTGCGTGTGGTCGGCATGCGCCGCAAGATCGCGGAGAAGATGGCGATTTCCAAACGCCAGA
TCCCGCATATCACCATCGTCGAAGAAATCGAGATGGAGGCGCTCGAAGATCTGCGGGCAGCATTGAACCGCAAATACAAG
GACCAGCGCCCCAAGCTCACGCTGCTGCCTTTCCTGATGCGTGCCATCGTGGAGGCGGTGCGTGAACAGCCGGAGCTCAA
TGCGCGCTACGATGATGAAAAGGGCATCATTCACCGCCACGGCGGCGTGCATGTCGGTATTGCGACCCAGACGCCGAACG
GGCTCAATGTACCCGTGGTGCATCACGCTGAGGCGGGTAGCCTGTGGGACAATGCGGCCGAACTCGCGCGCCTTGCAGAA
GCGGCGCGGGATGGATCGATCAAGCGGGATGAGCTCACGGGTGGCACCATCACCATTACCTCGCTTGGTGCGCTGGGCGC
GATTGCGACCACGCCGATCATCAACCACCCCGAAGTAGCCATTGTTGGGGTCAACAAAATGCAGATGCGCCCGGTTTGGG
ACGGTCAGCAATTCCAGCCCTGCAAGATGATGAATATCTCCTGCTCTTTCGACCACCGGGTGATCGACGGCTGGGATGCG
GCGGTGTTCGTGCAAAAACTGAAACTCCTGCTGGAAACGCCAGCGATGCTGTTTGTTGAGGGCTAG

Upstream 100 bases:

>100_bases
GGCTGGGATACGCCCTACCCGCATGCGCAGGAATGGAATTACTTCCCCGGCCCAGAACGGGTGGGCGAAGCGCTGAAGAA
ATGCATGGAGGACTGAGCGG

Downstream 100 bases:

>100_bases
ATGACTGATATAAAACGCAAAGTTCTGGTGATCGGCGCTGGCCCCGGCGGCTATGTCTGCGCCATTCGCGCTGGGCAGCT
CGGGCTCGATACGGTCATCG

Product: branched-chain alpha-keto acid dehydrogenase E2 component

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MGIFAIRLPDVGEGIAEAELTEWHVKPGDIVKEDDVLAAVMTDKAAVEVPSSVEGKVVELGGEIGDMLAIGCVLVRIEVD
GEGNEAASVAEPEPTPAPKEQKAEPEPERGDKPAEPARPVVKTGKPVARAKNTKPLAAPSVRARAREEGVDLRQVPGSGP
GGRISHGDLENWIASGGIQQGSVKRGKNTGVEDVRVVGMRRKIAEKMAISKRQIPHITIVEEIEMEALEDLRAALNRKYK
DQRPKLTLLPFLMRAIVEAVREQPELNARYDDEKGIIHRHGGVHVGIATQTPNGLNVPVVHHAEAGSLWDNAAELARLAE
AARDGSIKRDELTGGTITITSLGALGAIATTPIINHPEVAIVGVNKMQMRPVWDGQQFQPCKMMNISCSFDHRVIDGWDA
AVFVQKLKLLLETPAMLFVEG

Sequences:

>Translated_421_residues
MGIFAIRLPDVGEGIAEAELTEWHVKPGDIVKEDDVLAAVMTDKAAVEVPSSVEGKVVELGGEIGDMLAIGCVLVRIEVD
GEGNEAASVAEPEPTPAPKEQKAEPEPERGDKPAEPARPVVKTGKPVARAKNTKPLAAPSVRARAREEGVDLRQVPGSGP
GGRISHGDLENWIASGGIQQGSVKRGKNTGVEDVRVVGMRRKIAEKMAISKRQIPHITIVEEIEMEALEDLRAALNRKYK
DQRPKLTLLPFLMRAIVEAVREQPELNARYDDEKGIIHRHGGVHVGIATQTPNGLNVPVVHHAEAGSLWDNAAELARLAE
AARDGSIKRDELTGGTITITSLGALGAIATTPIINHPEVAIVGVNKMQMRPVWDGQQFQPCKMMNISCSFDHRVIDGWDA
AVFVQKLKLLLETPAMLFVEG
>Mature_420_residues
GIFAIRLPDVGEGIAEAELTEWHVKPGDIVKEDDVLAAVMTDKAAVEVPSSVEGKVVELGGEIGDMLAIGCVLVRIEVDG
EGNEAASVAEPEPTPAPKEQKAEPEPERGDKPAEPARPVVKTGKPVARAKNTKPLAAPSVRARAREEGVDLRQVPGSGPG
GRISHGDLENWIASGGIQQGSVKRGKNTGVEDVRVVGMRRKIAEKMAISKRQIPHITIVEEIEMEALEDLRAALNRKYKD
QRPKLTLLPFLMRAIVEAVREQPELNARYDDEKGIIHRHGGVHVGIATQTPNGLNVPVVHHAEAGSLWDNAAELARLAEA
ARDGSIKRDELTGGTITITSLGALGAIATTPIINHPEVAIVGVNKMQMRPVWDGQQFQPCKMMNISCSFDHRVIDGWDAA
VFVQKLKLLLETPAMLFVEG

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=443, Percent_Identity=29.5711060948081, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI31711992, Length=435, Percent_Identity=28.0459770114943, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI19923748, Length=224, Percent_Identity=33.0357142857143, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI203098816, Length=226, Percent_Identity=27.4336283185841, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI203098753, Length=226, Percent_Identity=27.4336283185841, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI260898739, Length=169, Percent_Identity=27.810650887574, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1786946, Length=429, Percent_Identity=29.8368298368298, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1786305, Length=422, Percent_Identity=32.2274881516588, Blast_Score=190, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17537937, Length=445, Percent_Identity=30.3370786516854, Blast_Score=201, Evalue=6e-52,
Organism=Caenorhabditis elegans, GI25146366, Length=420, Percent_Identity=30.2380952380952, Blast_Score=173, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17560088, Length=439, Percent_Identity=29.6127562642369, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17538894, Length=320, Percent_Identity=26.5625, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320352, Length=424, Percent_Identity=29.4811320754717, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6324258, Length=451, Percent_Identity=27.0509977827051, Blast_Score=146, Evalue=6e-36,
Organism=Drosophila melanogaster, GI18859875, Length=441, Percent_Identity=29.0249433106576, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI20129315, Length=431, Percent_Identity=27.1461716937355, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24582497, Length=417, Percent_Identity=27.5779376498801, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24645909, Length=230, Percent_Identity=33.0434782608696, Blast_Score=125, Evalue=6e-29,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 45404; Mature: 45272

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIFAIRLPDVGEGIAEAELTEWHVKPGDIVKEDDVLAAVMTDKAAVEVPSSVEGKVVEL
CCEEEEECCCCCCCHHHCCHHEEECCCCCCCCCCCEEEEEECCCHHEECCCCCCCEEEEE
GGEIGDMLAIGCVLVRIEVDGEGNEAASVAEPEPTPAPKEQKAEPEPERGDKPAEPARPV
CCHHHHHHEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
VKTGKPVARAKNTKPLAAPSVRARAREEGVDLRQVPGSGPGGRISHGDLENWIASGGIQQ
HHCCCCHHHCCCCCCCCCCHHHHHHHHCCCCEEECCCCCCCCCCCCCCHHHHHHHCCCCC
GSVKRGKNTGVEDVRVVGMRRKIAEKMAISKRQIPHITIVEEIEMEALEDLRAALNRKYK
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHCCHHH
DQRPKLTLLPFLMRAIVEAVREQPELNARYDDEKGIIHRHGGVHVGIATQTPNGLNVPVV
HCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCEEEEEEECCCCCCCCCEE
HHAEAGSLWDNAAELARLAEAARDGSIKRDELTGGTITITSLGALGAIATTPIINHPEVA
EECCCCCHHHHHHHHHHHHHHHHCCCCCHHCCCCCEEEEEECCCHHHHHHCCCCCCCCEE
IVGVNKMQMRPVWDGQQFQPCKMMNISCSFDHRVIDGWDAAVFVQKLKLLLETPAMLFVE
EEECCCEEECCCCCCCCCCCEEEEEEEECCCCEEECCCHHHHHHHHHHHHHCCCCEEEEC
G
C
>Mature Secondary Structure 
GIFAIRLPDVGEGIAEAELTEWHVKPGDIVKEDDVLAAVMTDKAAVEVPSSVEGKVVEL
CEEEEECCCCCCCHHHCCHHEEECCCCCCCCCCCEEEEEECCCHHEECCCCCCCEEEEE
GGEIGDMLAIGCVLVRIEVDGEGNEAASVAEPEPTPAPKEQKAEPEPERGDKPAEPARPV
CCHHHHHHEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
VKTGKPVARAKNTKPLAAPSVRARAREEGVDLRQVPGSGPGGRISHGDLENWIASGGIQQ
HHCCCCHHHCCCCCCCCCCHHHHHHHHCCCCEEECCCCCCCCCCCCCCHHHHHHHCCCCC
GSVKRGKNTGVEDVRVVGMRRKIAEKMAISKRQIPHITIVEEIEMEALEDLRAALNRKYK
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHCCHHH
DQRPKLTLLPFLMRAIVEAVREQPELNARYDDEKGIIHRHGGVHVGIATQTPNGLNVPVV
HCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCEEEEEEECCCCCCCCCEE
HHAEAGSLWDNAAELARLAEAARDGSIKRDELTGGTITITSLGALGAIATTPIINHPEVA
EECCCCCHHHHHHHHHHHHHHHHCCCCCHHCCCCCEEEEEECCCHHHHHHCCCCCCCCEE
IVGVNKMQMRPVWDGQQFQPCKMMNISCSFDHRVIDGWDAAVFVQKLKLLLETPAMLFVE
EEECCCEEECCCCCCCCCCCEEEEEEEECCCCEEECCCHHHHHHHHHHHHHCCCCEEEEC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3046941 [H]