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The map label for this gene is lpdV [H]

Identifier: 99082615

GI number: 99082615

Start: 2933253

End: 2934611

Strand: Reverse

Name: lpdV [H]

Synonym: TM1040_2775

Alternate gene names: 99082615

Gene position: 2934611-2933253 (Counterclockwise)

Preceding gene: 99082616

Following gene: 99082613

Centisome position: 91.68

GC content: 60.85

Gene sequence:

>1359_bases
ATGACTGATATAAAACGCAAAGTTCTGGTGATCGGCGCTGGCCCCGGCGGCTATGTCTGCGCCATTCGCGCTGGGCAGCT
CGGGCTCGATACGGTCATCGTCGATGAGGCCCGCCCCGGTGGCACCTGTCTCAATATCGGCTGTATTCCGTCCAAGGCGA
TGATCCATGCCGCCGACGAGTTCTACAAAATGACCCATGCAGGCGCAGGCCCGCTTGGGATTTCGGCCGCAGGCCCGACT
ATTGATCTTGAGCAAACCGTTGCGTGGAAAGATGGCATTGTAGAGCGCTTGACTGGCGGGGTAGGCGGGCTCTTGCGTAA
AGCAGGCGTCAAGATGATCTCAGGCCGCGCCCGCTTTGTGGATGGCAAGACTGTTACCGTTGCAGGCGACGAGGGCGAGA
TACAGATCCGTGCCGACTACATCGTGATCGCAACCGGGTCGGCCCCGGTGGAACTGCCCTTCCTGCCATTTGGGGGGGCG
GTGATGTCTTCGACCGAAGCGCTCTCCCTGACCGAAGTGCCCGAGAGCCTAGCCGTGGTCGGCGGCGGCTATATCGGCCT
CGAACTTGGCACCGTGTTTGCAAAACTTGGGTCCAAGGTCACCGTGGTTGAGGCCGAGGACCGTATACTGCCGCTATATG
ACAAGGCGCTGACACGTCCTGTTCACAAACGCCTTGAGGAGCTCGGCGTGACGGTCATGACGGGCACCAAAGCGCAGTCA
CATGAGGGCGGCGCGCTGCAAACTGATGCTGGTGAAATCGCTGCAGAGCGTGTGCTTGTGACGGTCGGGCGCAGGCCGCG
CACTCAGGGCATTGGTATTGATGAGTTGTCTCTGACGATGAACGGGCCTTTCATCCGCATCGACAAGGTCTGCCAGACCT
CGATGCGGGGGATCTATGCAATCGGGGACGTGACCGGAGAGCCCATGCTTGCCCATCGTGCGATGGCGCAGGGGGAGATG
GTGGCGGAATATCTGGCCGGGCAAGCGGTAGAGTGGGACAAGCGCGCGATCCCTGCCGTGTGTTTTACCGATCCGGAGAT
TGTCACCTGTGGCGTGCTCCCCGGCGAGGTCGAGGGCACCAAATCGGCCGAGTTTCCGTTTGTGGCCAATGGTCGTGCGA
TGACAACCGAACGCGATGAGGGTTTCATTCGGGTGGTCTGGCGCGAAGCGGACAATGCGGTTCTGGGGCTGCAGGGTGTC
GGCGTGGGCATATCCGAGCTCTCTTCGGCGTTTTCGCTCGCCATCGAGATGGGCGCCTGCCTTGAGGATATCGCCGCCAC
CATCCATGCGCATCCAACACAGTCCGAGGGTTTGCAAGAGGCCTGTCTGAAGGCGCTTGGTCACGCGTTGCACATCTGA

Upstream 100 bases:

>100_bases
TTTCGACCACCGGGTGATCGACGGCTGGGATGCGGCGGTGTTCGTGCAAAAACTGAAACTCCTGCTGGAAACGCCAGCGA
TGCTGTTTGTTGAGGGCTAG

Downstream 100 bases:

>100_bases
CAGCGAGAGCGCAGAAATCTGGGCCGCCTACGTGTCAGGCGGTCTCAGCCTGTGGCTTGCAGATCATCGCCTTCGCCTTG
CAGCGGAGCCACGGCCGTGG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of branched-chain alpha-keto acid dehydrogenase complex; LPD-Val [H]

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MTDIKRKVLVIGAGPGGYVCAIRAGQLGLDTVIVDEARPGGTCLNIGCIPSKAMIHAADEFYKMTHAGAGPLGISAAGPT
IDLEQTVAWKDGIVERLTGGVGGLLRKAGVKMISGRARFVDGKTVTVAGDEGEIQIRADYIVIATGSAPVELPFLPFGGA
VMSSTEALSLTEVPESLAVVGGGYIGLELGTVFAKLGSKVTVVEAEDRILPLYDKALTRPVHKRLEELGVTVMTGTKAQS
HEGGALQTDAGEIAAERVLVTVGRRPRTQGIGIDELSLTMNGPFIRIDKVCQTSMRGIYAIGDVTGEPMLAHRAMAQGEM
VAEYLAGQAVEWDKRAIPAVCFTDPEIVTCGVLPGEVEGTKSAEFPFVANGRAMTTERDEGFIRVVWREADNAVLGLQGV
GVGISELSSAFSLAIEMGACLEDIAATIHAHPTQSEGLQEACLKALGHALHI

Sequences:

>Translated_452_residues
MTDIKRKVLVIGAGPGGYVCAIRAGQLGLDTVIVDEARPGGTCLNIGCIPSKAMIHAADEFYKMTHAGAGPLGISAAGPT
IDLEQTVAWKDGIVERLTGGVGGLLRKAGVKMISGRARFVDGKTVTVAGDEGEIQIRADYIVIATGSAPVELPFLPFGGA
VMSSTEALSLTEVPESLAVVGGGYIGLELGTVFAKLGSKVTVVEAEDRILPLYDKALTRPVHKRLEELGVTVMTGTKAQS
HEGGALQTDAGEIAAERVLVTVGRRPRTQGIGIDELSLTMNGPFIRIDKVCQTSMRGIYAIGDVTGEPMLAHRAMAQGEM
VAEYLAGQAVEWDKRAIPAVCFTDPEIVTCGVLPGEVEGTKSAEFPFVANGRAMTTERDEGFIRVVWREADNAVLGLQGV
GVGISELSSAFSLAIEMGACLEDIAATIHAHPTQSEGLQEACLKALGHALHI
>Mature_451_residues
TDIKRKVLVIGAGPGGYVCAIRAGQLGLDTVIVDEARPGGTCLNIGCIPSKAMIHAADEFYKMTHAGAGPLGISAAGPTI
DLEQTVAWKDGIVERLTGGVGGLLRKAGVKMISGRARFVDGKTVTVAGDEGEIQIRADYIVIATGSAPVELPFLPFGGAV
MSSTEALSLTEVPESLAVVGGGYIGLELGTVFAKLGSKVTVVEAEDRILPLYDKALTRPVHKRLEELGVTVMTGTKAQSH
EGGALQTDAGEIAAERVLVTVGRRPRTQGIGIDELSLTMNGPFIRIDKVCQTSMRGIYAIGDVTGEPMLAHRAMAQGEMV
AEYLAGQAVEWDKRAIPAVCFTDPEIVTCGVLPGEVEGTKSAEFPFVANGRAMTTERDEGFIRVVWREADNAVLGLQGVG
VGISELSSAFSLAIEMGACLEDIAATIHAHPTQSEGLQEACLKALGHALHI

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=464, Percent_Identity=37.0689655172414, Blast_Score=258, Evalue=1e-68,
Organism=Homo sapiens, GI50301238, Length=460, Percent_Identity=29.1304347826087, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI22035672, Length=460, Percent_Identity=29.1304347826087, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI148277065, Length=467, Percent_Identity=24.8394004282655, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI33519430, Length=467, Percent_Identity=24.8394004282655, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI33519428, Length=467, Percent_Identity=24.8394004282655, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI33519426, Length=467, Percent_Identity=24.8394004282655, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI148277071, Length=467, Percent_Identity=24.8394004282655, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI291045266, Length=467, Percent_Identity=25.0535331905782, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI291045268, Length=455, Percent_Identity=23.956043956044, Blast_Score=92, Evalue=7e-19,
Organism=Escherichia coli, GI1786307, Length=453, Percent_Identity=38.1898454746137, Blast_Score=300, Evalue=2e-82,
Organism=Escherichia coli, GI1789915, Length=431, Percent_Identity=29.4663573085847, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI87081717, Length=452, Percent_Identity=25.2212389380531, Blast_Score=138, Evalue=7e-34,
Organism=Escherichia coli, GI87082354, Length=459, Percent_Identity=24.8366013071895, Blast_Score=130, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI32565766, Length=457, Percent_Identity=36.7614879649891, Blast_Score=257, Evalue=7e-69,
Organism=Caenorhabditis elegans, GI17557007, Length=468, Percent_Identity=26.7094017094017, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI71983429, Length=446, Percent_Identity=28.47533632287, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI71983419, Length=446, Percent_Identity=28.47533632287, Blast_Score=125, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6321091, Length=472, Percent_Identity=35.8050847457627, Blast_Score=242, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6325166, Length=460, Percent_Identity=29.5652173913043, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6325240, Length=480, Percent_Identity=28.75, Blast_Score=155, Evalue=1e-38,
Organism=Drosophila melanogaster, GI21358499, Length=458, Percent_Identity=39.0829694323144, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24640553, Length=470, Percent_Identity=27.6595744680851, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24640551, Length=470, Percent_Identity=27.6595744680851, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24640549, Length=470, Percent_Identity=27.6595744680851, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI17737741, Length=466, Percent_Identity=25.5364806866953, Blast_Score=108, Evalue=9e-24,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 47326; Mature: 47195

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIKRKVLVIGAGPGGYVCAIRAGQLGLDTVIVDEARPGGTCLNIGCIPSKAMIHAADE
CCCCCEEEEEEECCCCCEEEEEEECCCCCEEEEEECCCCCCCEEEEECCCCHHHHHHHHH
FYKMTHAGAGPLGISAAGPTIDLEQTVAWKDGIVERLTGGVGGLLRKAGVKMISGRARFV
HHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEECCCEEEE
DGKTVTVAGDEGEIQIRADYIVIATGSAPVELPFLPFGGAVMSSTEALSLTEVPESLAVV
CCCEEEEECCCCEEEEEEEEEEEEECCCCEEEEEECCCCHHHCCCCCEEHHHCCCHHEEE
GGGYIGLELGTVFAKLGSKVTVVEAEDRILPLYDKALTRPVHKRLEELGVTVMTGTKAQS
CCCEEEHHHHHHHHHHCCEEEEEECCCCEEEHHHHHHHHHHHHHHHHCCEEEEECCCCCC
HEGGALQTDAGEIAAERVLVTVGRRPRTQGIGIDELSLTMNGPFIRIDKVCQTSMRGIYA
CCCCCEECCHHHHHHHEEEEEECCCCCCCCCCCEEEEEEECCCEEEEHHHHHHHCCCEEE
IGDVTGEPMLAHRAMAQGEMVAEYLAGQAVEWDKRAIPAVCFTDPEIVTCGVLPGEVEGT
EECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCCCC
KSAEFPFVANGRAMTTERDEGFIRVVWREADNAVLGLQGVGVGISELSSAFSLAIEMGAC
CCCCCCEEECCEEEEEECCCCEEEEEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHH
LEDIAATIHAHPTQSEGLQEACLKALGHALHI
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDIKRKVLVIGAGPGGYVCAIRAGQLGLDTVIVDEARPGGTCLNIGCIPSKAMIHAADE
CCCCEEEEEEECCCCCEEEEEEECCCCCEEEEEECCCCCCCEEEEECCCCHHHHHHHHH
FYKMTHAGAGPLGISAAGPTIDLEQTVAWKDGIVERLTGGVGGLLRKAGVKMISGRARFV
HHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEECCCEEEE
DGKTVTVAGDEGEIQIRADYIVIATGSAPVELPFLPFGGAVMSSTEALSLTEVPESLAVV
CCCEEEEECCCCEEEEEEEEEEEEECCCCEEEEEECCCCHHHCCCCCEEHHHCCCHHEEE
GGGYIGLELGTVFAKLGSKVTVVEAEDRILPLYDKALTRPVHKRLEELGVTVMTGTKAQS
CCCEEEHHHHHHHHHHCCEEEEEECCCCEEEHHHHHHHHHHHHHHHHCCEEEEECCCCCC
HEGGALQTDAGEIAAERVLVTVGRRPRTQGIGIDELSLTMNGPFIRIDKVCQTSMRGIYA
CCCCCEECCHHHHHHHEEEEEECCCCCCCCCCCEEEEEEECCCEEEEHHHHHHHCCCEEE
IGDVTGEPMLAHRAMAQGEMVAEYLAGQAVEWDKRAIPAVCFTDPEIVTCGVLPGEVEGT
EECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCCCC
KSAEFPFVANGRAMTTERDEGFIRVVWREADNAVLGLQGVGVGISELSSAFSLAIEMGAC
CCCCCCEEECCEEEEEECCCCEEEEEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHH
LEDIAATIHAHPTQSEGLQEACLKALGHALHI
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3046941; 2917566; 1325638 [H]