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The map label for this gene is gacS [H]

Identifier: 99082614

GI number: 99082614

Start: 2930923

End: 2933208

Strand: Direct

Name: gacS [H]

Synonym: TM1040_2774

Alternate gene names: 99082614

Gene position: 2930923-2933208 (Clockwise)

Preceding gene: 99082610

Following gene: 99082620

Centisome position: 91.56

GC content: 58.09

Gene sequence:

>2286_bases
ATGTCGCTTGCTGAAAAATTGGCTCAGGAACGCAGGGCGCGATTGGCAGCCGAGCGACTTCTGGAGCTGAAACAAGCTGA
ACTGACCGATGCGAACCGCAAGCTCGGCCGCCATGCGCTTGCGCTAACCAAACGGATCGGCGCCACTCAGGCAGAAATCG
CGACCTTCAAGAACGAAAATGAAAAGGTCAAAAGCGACCTCAACACAGCCAATGAAAAGGTCGAGCAGGCCGAACGGCGC
CTGTGGCACTCTATTCAGGCCTTTCAGGACGGTTTTGCCTTCTTTGACAGTGACAGCAAGTTGATCGGCGCCAACACTGC
CTATCTCAATATCTTTGAGGGGGTAGAGGAGGTATCGCCGGGCGTCTCCTATGTGCGCATCCTGCAAATCCTGACCGATG
AGGGGCTGATCGACACCGAGGAGCTCTCGGCGGATTCGTGGCGCGCGATGATGACGGAGCGCTGGATGTCGCCCGCGCCA
GAACCAACGGTGGTGCGGCTGTGGAATGATCGCTACCTGAAGCTGATCGACCAACGCGGCCATGACGGTGACATCGTGAG
TCTCGCCCTCGATATCACCGCAACTGTCCACTACGAGGAAGAGCTTCGCAGCGCCCGAGAGCGCGCCGAGGCTGCGAATC
GCGCGAAATCCGCCTTTCTGGCCAATATGAGCCACGAGATCCGTACGCCAATGAACGGTGTCGTCGGCATGGCCGAGCTC
CTGAGCGACACCACGCTGACCGAAGAGCAGCGCCTTTATGCCAATACAATCAAGAACTCGGGCGAGGCGCTTCTGGTCAT
CATCAACGACGTTCTCGACTATTCCAAGATCGAGGCGGACAAATTGGCGCTGCATCCCGAGGCCTTTGATCTTGAAGCCT
GCATGCATGAGGTCGTCATGCTGCTGCAGGCCAATGCCCGCGACAAAGGGCTGACGCTGCTGGTTGACTACGACCTCTTT
ATGCCGACGAATTTCATTGGCGACCCAGGGCGCATCCGCCAAATCCTCACCAATCTTATCGGCAATGCGATCAAATTCAC
CAACGAAGGGCATGTTACGGCTCGCGTGACCGGGGTGCCGCATCCCGAAGACAATACCGTGATGCTGCATATCTCGATCG
AGGACAGCGGCATCGGCATTCCTGACGACAAGATTCAGCACATCTTTGGCGAATTCAATCAGGTCGAGGATGAAACCAAC
CGGCAGTTTGAGGGCACGGGGCTTGGTCTCGCGATCACCGAACGCCTGATCAAGTTGATGGACGGCGAGATCTGGGTCGA
AAGCCAGTTGGGCAAAGGCTCGTGTTTTGGCTTCCGCCTGCCGCTTGTGGTGGCGGATGGCTCACAGGTGAATGCGCCCG
AACTGCCCGAAAACCTGAACTGCGTGATGATCGTCGATGATATGGATATCAACCGCGAGATCCTGAACCGTCAACTTCAG
CGCCTGGACCTCAAGGTGGTGGCCGTAACCAGCGGTGCCGAGGCACTCGACGTTCTCAATGCCGAAATCGATTTGGTCAT
CACCGATCGCAACCTACCGGGGATGGACGGGCTGAAACTGGCGCGCAGCCTGCACCATGCTTCGCCACAGACGCCGGTAC
TGCTGCTTTCTTCTGATCCTGCTGATGGGAGCGACCCTGCCCTCAGCGATCTCTTTGCTGGCATTCTGCAGAAACCGATT
CCTCGCGGCGCCCTGTTCCGAGCCCTCTCCAAACTTGATGCGCCATCTGTACATCCCGCCCGACCGCAAGATGTGCCACC
GGCTGCTCCCGCCATCGAGCCGCCTTCAGACGCCCCCGTGCAAGTGCCTGCACCCGAAGCGGGCGTGGACTTCGGATCCG
GCGCGACGATCGCTTCGGATCTGCGCCGGATGCGGGTTCTGGCGGCGGAGGACAACCGCACCAACCAGCTGGTCTTCCGC
AAGATGGTCAAGGATATGAACATCGACTTGCGCTTTGCAGGCAACGGGTTGGAGGCGGTTGAGCTCTACCAGAGTTTTAA
ACCGGATATGATTTTCATGGATATTTCCATGCCCAAAATGGATGGCAAAGAGGCCACGCAGGCCATTCGCGAGCTTGAAA
AAGAAAACGGTCGCAGAGTGCAGATAGTGGCCTTGACGGCGCATGCGATGGATGGCGATTCCGAGGGCATACTGGCCGCA
GGCCTTGACGACTACCTCACCAAGCCCCTGCGGAAATCGGTGATCCACGACCGGATCATGAAAAACATGCCCACGGCCGT
GGCTCCGCTGCAAGGCGAAGGCGATGATCTGCAAGCCACAGGCTGA

Upstream 100 bases:

>100_bases
AAGCATGCGCGCCATAACACCAACGCTAAAGGCAAATTAAGGAATGCCTGCGAGATTGGTGCCGCGGGCAGGGAAACAAC
TAGTGTTGGAGTTGGCGCAT

Downstream 100 bases:

>100_bases
GACCGCCTGACACGTAGGCGGCCCAGATTTCTGCGCTCTCGCTGTCAGATGTGCAACGCGTGACCAAGCGCCTTCAGACA
GGCCTCTTGCAAACCCTCGG

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 761; Mature: 760

Protein sequence:

>761_residues
MSLAEKLAQERRARLAAERLLELKQAELTDANRKLGRHALALTKRIGATQAEIATFKNENEKVKSDLNTANEKVEQAERR
LWHSIQAFQDGFAFFDSDSKLIGANTAYLNIFEGVEEVSPGVSYVRILQILTDEGLIDTEELSADSWRAMMTERWMSPAP
EPTVVRLWNDRYLKLIDQRGHDGDIVSLALDITATVHYEEELRSARERAEAANRAKSAFLANMSHEIRTPMNGVVGMAEL
LSDTTLTEEQRLYANTIKNSGEALLVIINDVLDYSKIEADKLALHPEAFDLEACMHEVVMLLQANARDKGLTLLVDYDLF
MPTNFIGDPGRIRQILTNLIGNAIKFTNEGHVTARVTGVPHPEDNTVMLHISIEDSGIGIPDDKIQHIFGEFNQVEDETN
RQFEGTGLGLAITERLIKLMDGEIWVESQLGKGSCFGFRLPLVVADGSQVNAPELPENLNCVMIVDDMDINREILNRQLQ
RLDLKVVAVTSGAEALDVLNAEIDLVITDRNLPGMDGLKLARSLHHASPQTPVLLLSSDPADGSDPALSDLFAGILQKPI
PRGALFRALSKLDAPSVHPARPQDVPPAAPAIEPPSDAPVQVPAPEAGVDFGSGATIASDLRRMRVLAAEDNRTNQLVFR
KMVKDMNIDLRFAGNGLEAVELYQSFKPDMIFMDISMPKMDGKEATQAIRELEKENGRRVQIVALTAHAMDGDSEGILAA
GLDDYLTKPLRKSVIHDRIMKNMPTAVAPLQGEGDDLQATG

Sequences:

>Translated_761_residues
MSLAEKLAQERRARLAAERLLELKQAELTDANRKLGRHALALTKRIGATQAEIATFKNENEKVKSDLNTANEKVEQAERR
LWHSIQAFQDGFAFFDSDSKLIGANTAYLNIFEGVEEVSPGVSYVRILQILTDEGLIDTEELSADSWRAMMTERWMSPAP
EPTVVRLWNDRYLKLIDQRGHDGDIVSLALDITATVHYEEELRSARERAEAANRAKSAFLANMSHEIRTPMNGVVGMAEL
LSDTTLTEEQRLYANTIKNSGEALLVIINDVLDYSKIEADKLALHPEAFDLEACMHEVVMLLQANARDKGLTLLVDYDLF
MPTNFIGDPGRIRQILTNLIGNAIKFTNEGHVTARVTGVPHPEDNTVMLHISIEDSGIGIPDDKIQHIFGEFNQVEDETN
RQFEGTGLGLAITERLIKLMDGEIWVESQLGKGSCFGFRLPLVVADGSQVNAPELPENLNCVMIVDDMDINREILNRQLQ
RLDLKVVAVTSGAEALDVLNAEIDLVITDRNLPGMDGLKLARSLHHASPQTPVLLLSSDPADGSDPALSDLFAGILQKPI
PRGALFRALSKLDAPSVHPARPQDVPPAAPAIEPPSDAPVQVPAPEAGVDFGSGATIASDLRRMRVLAAEDNRTNQLVFR
KMVKDMNIDLRFAGNGLEAVELYQSFKPDMIFMDISMPKMDGKEATQAIRELEKENGRRVQIVALTAHAMDGDSEGILAA
GLDDYLTKPLRKSVIHDRIMKNMPTAVAPLQGEGDDLQATG
>Mature_760_residues
SLAEKLAQERRARLAAERLLELKQAELTDANRKLGRHALALTKRIGATQAEIATFKNENEKVKSDLNTANEKVEQAERRL
WHSIQAFQDGFAFFDSDSKLIGANTAYLNIFEGVEEVSPGVSYVRILQILTDEGLIDTEELSADSWRAMMTERWMSPAPE
PTVVRLWNDRYLKLIDQRGHDGDIVSLALDITATVHYEEELRSARERAEAANRAKSAFLANMSHEIRTPMNGVVGMAELL
SDTTLTEEQRLYANTIKNSGEALLVIINDVLDYSKIEADKLALHPEAFDLEACMHEVVMLLQANARDKGLTLLVDYDLFM
PTNFIGDPGRIRQILTNLIGNAIKFTNEGHVTARVTGVPHPEDNTVMLHISIEDSGIGIPDDKIQHIFGEFNQVEDETNR
QFEGTGLGLAITERLIKLMDGEIWVESQLGKGSCFGFRLPLVVADGSQVNAPELPENLNCVMIVDDMDINREILNRQLQR
LDLKVVAVTSGAEALDVLNAEIDLVITDRNLPGMDGLKLARSLHHASPQTPVLLLSSDPADGSDPALSDLFAGILQKPIP
RGALFRALSKLDAPSVHPARPQDVPPAAPAIEPPSDAPVQVPAPEAGVDFGSGATIASDLRRMRVLAAEDNRTNQLVFRK
MVKDMNIDLRFAGNGLEAVELYQSFKPDMIFMDISMPKMDGKEATQAIRELEKENGRRVQIVALTAHAMDGDSEGILAAG
LDDYLTKPLRKSVIHDRIMKNMPTAVAPLQGEGDDLQATG

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI145693157, Length=557, Percent_Identity=29.802513464991, Blast_Score=218, Evalue=1e-57,
Organism=Escherichia coli, GI48994928, Length=589, Percent_Identity=29.8811544991511, Blast_Score=218, Evalue=1e-57,
Organism=Escherichia coli, GI87081816, Length=386, Percent_Identity=38.60103626943, Blast_Score=217, Evalue=2e-57,
Organism=Escherichia coli, GI1789149, Length=242, Percent_Identity=47.1074380165289, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1788713, Length=395, Percent_Identity=35.4430379746835, Blast_Score=188, Evalue=9e-49,
Organism=Escherichia coli, GI1786600, Length=230, Percent_Identity=31.304347826087, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI87082128, Length=239, Percent_Identity=28.4518828451883, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1786912, Length=279, Percent_Identity=29.0322580645161, Blast_Score=88, Evalue=3e-18,
Organism=Escherichia coli, GI1786783, Length=239, Percent_Identity=27.1966527196653, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1790436, Length=223, Percent_Identity=29.1479820627803, Blast_Score=84, Evalue=4e-17,
Organism=Escherichia coli, GI1788393, Length=233, Percent_Identity=27.0386266094421, Blast_Score=77, Evalue=5e-15,
Organism=Escherichia coli, GI1788549, Length=226, Percent_Identity=25.2212389380531, Blast_Score=70, Evalue=7e-13,
Organism=Escherichia coli, GI1790346, Length=216, Percent_Identity=27.7777777777778, Blast_Score=67, Evalue=5e-12,
Organism=Escherichia coli, GI1790861, Length=244, Percent_Identity=25, Blast_Score=67, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6323034, Length=192, Percent_Identity=29.6875, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6322044, Length=115, Percent_Identity=34.7826086956522, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 83839; Mature: 83707

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLAEKLAQERRARLAAERLLELKQAELTDANRKLGRHALALTKRIGATQAEIATFKNEN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCC
EKVKSDLNTANEKVEQAERRLWHSIQAFQDGFAFFDSDSKLIGANTAYLNIFEGVEEVSP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEECCHHHHHHHHHHHHHCC
GVSYVRILQILTDEGLIDTEELSADSWRAMMTERWMSPAPEPTVVRLWNDRYLKLIDQRG
CHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHCCC
HDGDIVSLALDITATVHYEEELRSARERAEAANRAKSAFLANMSHEIRTPMNGVVGMAEL
CCCCEEEEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCHHHHHHHHHH
LSDTTLTEEQRLYANTIKNSGEALLVIINDVLDYSKIEADKLALHPEAFDLEACMHEVVM
HCCCCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHCCHHHEEECCCCCCHHHHHHHHHH
LLQANARDKGLTLLVDYDLFMPTNFIGDPGRIRQILTNLIGNAIKFTNEGHVTARVTGVP
HHHCCCCCCCEEEEEEEHHHCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCEEEEEECCC
HPEDNTVMLHISIEDSGIGIPDDKIQHIFGEFNQVEDETNRQFEGTGLGLAITERLIKLM
CCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHH
DGEIWVESQLGKGSCFGFRLPLVVADGSQVNAPELPENLNCVMIVDDMDINREILNRQLQ
CCCEEEECCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHH
RLDLKVVAVTSGAEALDVLNAEIDLVITDRNLPGMDGLKLARSLHHASPQTPVLLLSSDP
HCCEEEEEEECCCHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEECCC
ADGSDPALSDLFAGILQKPIPRGALFRALSKLDAPSVHPARPQDVPPAAPAIEPPSDAPV
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
QVPAPEAGVDFGSGATIASDLRRMRVLAAEDNRTNQLVFRKMVKDMNIDLRFAGNGLEAV
ECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEEECCCCHHHH
ELYQSFKPDMIFMDISMPKMDGKEATQAIRELEKENGRRVQIVALTAHAMDGDSEGILAA
HHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCEEEE
GLDDYLTKPLRKSVIHDRIMKNMPTAVAPLQGEGDDLQATG
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SLAEKLAQERRARLAAERLLELKQAELTDANRKLGRHALALTKRIGATQAEIATFKNEN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCC
EKVKSDLNTANEKVEQAERRLWHSIQAFQDGFAFFDSDSKLIGANTAYLNIFEGVEEVSP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEECCHHHHHHHHHHHHHCC
GVSYVRILQILTDEGLIDTEELSADSWRAMMTERWMSPAPEPTVVRLWNDRYLKLIDQRG
CHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHCCC
HDGDIVSLALDITATVHYEEELRSARERAEAANRAKSAFLANMSHEIRTPMNGVVGMAEL
CCCCEEEEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCHHHHHHHHHH
LSDTTLTEEQRLYANTIKNSGEALLVIINDVLDYSKIEADKLALHPEAFDLEACMHEVVM
HCCCCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHCCHHHEEECCCCCCHHHHHHHHHH
LLQANARDKGLTLLVDYDLFMPTNFIGDPGRIRQILTNLIGNAIKFTNEGHVTARVTGVP
HHHCCCCCCCEEEEEEEHHHCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCEEEEEECCC
HPEDNTVMLHISIEDSGIGIPDDKIQHIFGEFNQVEDETNRQFEGTGLGLAITERLIKLM
CCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHH
DGEIWVESQLGKGSCFGFRLPLVVADGSQVNAPELPENLNCVMIVDDMDINREILNRQLQ
CCCEEEECCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHH
RLDLKVVAVTSGAEALDVLNAEIDLVITDRNLPGMDGLKLARSLHHASPQTPVLLLSSDP
HCCEEEEEEECCCHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEECCC
ADGSDPALSDLFAGILQKPIPRGALFRALSKLDAPSVHPARPQDVPPAAPAIEPPSDAPV
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
QVPAPEAGVDFGSGATIASDLRRMRVLAAEDNRTNQLVFRKMVKDMNIDLRFAGNGLEAV
ECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEEECCCCHHHH
ELYQSFKPDMIFMDISMPKMDGKEATQAIRELEKENGRRVQIVALTAHAMDGDSEGILAA
HHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCEEEE
GLDDYLTKPLRKSVIHDRIMKNMPTAVAPLQGEGDDLQATG
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]