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The map label for this gene is appA [H]

Identifier: 99082585

GI number: 99082585

Start: 2889841

End: 2891496

Strand: Reverse

Name: appA [H]

Synonym: TM1040_2745

Alternate gene names: 99082585

Gene position: 2891496-2889841 (Counterclockwise)

Preceding gene: 99082587

Following gene: 99082584

Centisome position: 90.33

GC content: 58.7

Gene sequence:

>1656_bases
ATGAAATTCACTGCTACCGCCGGGCTCTTGGCCTCGGTTGCGCTCTGGACCACTGCCGCACAGGCAGAGGAAACCGTGCT
GAGCGCGTTGCCGGAACAGGTCACCGCTTGGGTGGAGAACTTCAACCCGTTCAACCAGACCACCGCGGCGCCGTCCGTCA
TGCATTTCATGTACGAGCCGCTGATCATTTTCAACGCGCTCGACGGCGGCAAGCCGATCTACCGTCTGGCGACCGCATTT
GAGTATTCCGATGATCTGAGTTCCATCACCGTGACCCTGCGCGATGGCGTTCAGTGGTCCGATGGCGAAGCATTCACCGC
CGATGATGTGGTCAAATCCTTTGATCTGGCGCTGAGCGATCCGGCCCTCGACAGCGTCGGCATGGCGCAGATGCTCTCTG
GTGTCGAGAAACTCGACGAGATGACGGTGAAGTTCAACCTCTCCACCCCTTCAAGCCAGGCCATGTACCAGATCGTGCGT
GTGCCAATCGTCCCCGAACACGTCTGGAGCAACGTCTCCGATCCCGTGACCTTTACCAACCCTGATCCCGTGGGGTCCGG
TCCGCTGACAGAGATCCGCCGTTTCACGCCGCAGGAATACATCCAGTGCCGCAACAACAATTACTGGGACGCTGAGAGCC
TCAAGGTCGACTGCATGCGTTTCCCGCAGATCGCCAACAACGATCAGGCGCTGGCAGCGGCCGCGAATGGCGAACTGGAC
TGGATGGGGTCCTTCCTGCCCGACATCGACAACACCTTTGTCGCCAAGGACCCCGAGCATCACAGCTATTGGCTGCCCGC
AGGCTCTCTCGTGGCGTTCTACATGAACTTCGAGGCCAAAGAAGCCGGCGACAAAGAAGCCGTGAACAACGTCGCCTTCC
GCCGCGCGGTGTCGATGGCCTTCGACCGCGAAGCGATGGTGGAAATTGCAGGCTATGGCTATCCGACGATCAACCAGTAT
CCCTCTGGTCTGGGCCGCGCTTATCACGCGTGGAACAACCCCGAAGTCGAGGACAAATTTGGCGCGTTTACCCAATATGA
CATCGAGGGCGCCAAGGCACAGCTGGCCGAGGCCGGGTTCAAGGACATTGACGGTGACGGCTTTGTGGAAACCCCAAGCG
GTGAGCAGATCGACATTGAAGTCATCGTGCCCAACGGCTGGACCGACTGGGTCAACAGCAGCCAGATCGCGGTCGAGGGC
CTGAATGCAGCCGGGATCAAGGCCAATGTCTCAACACCTGAATCCGCGATCTGGACCGAAAAGCTGATCAAGGGCGACTA
TGACATGGCGATCAACTCGGTTCGTGTTGGTGCGACCCCCTTCAACCAGTATCTGGACTCGCTCCACGAGATTAATCAGG
CCAAGTCGCGTTTTGCCGCGTCGCGGTACTACAACGAAGAGCTGAGCGACCTTCTGGATGCCTTCACCCAGACCAGCGAC
ACCGACAAGCAGATGGCGATCATGTCCGATGTACAAGAGATCGTCGGTGAAGACATGCCGCTGGCCTATGTGTTCAACAA
CCCGCGCTGGTATCAGTACAACACCAAGCGTTTCGAAGGCTTCTTCAACGCTGACAACCCGGTGGCCAACCCGGTGGTTC
ACAAAACCAACCCGGCCCGTCTGATCCACCTCCTGAACCTGCGCCCGGTCGAGTAA

Upstream 100 bases:

>100_bases
ATCAAATGCCCCCCATGGTTTGCTCCGTGGGCGCCCTTCACCGGGCGGGAACGGGGAATGACACGAGGCGGGAAAACCCG
TCATCACAGGGAGACACAAC

Downstream 100 bases:

>100_bases
TTCCTCCCCGACCTTCGGGACCGCTCATCTGATGAGTGGTCCCGCTTTTGGCGCCCCACACTCCCCTCCCGACAGATGGT
TTGACCTGATGCGTTTTCTT

Product: extracellular solute-binding protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 551; Mature: 551

Protein sequence:

>551_residues
MKFTATAGLLASVALWTTAAQAEETVLSALPEQVTAWVENFNPFNQTTAAPSVMHFMYEPLIIFNALDGGKPIYRLATAF
EYSDDLSSITVTLRDGVQWSDGEAFTADDVVKSFDLALSDPALDSVGMAQMLSGVEKLDEMTVKFNLSTPSSQAMYQIVR
VPIVPEHVWSNVSDPVTFTNPDPVGSGPLTEIRRFTPQEYIQCRNNNYWDAESLKVDCMRFPQIANNDQALAAAANGELD
WMGSFLPDIDNTFVAKDPEHHSYWLPAGSLVAFYMNFEAKEAGDKEAVNNVAFRRAVSMAFDREAMVEIAGYGYPTINQY
PSGLGRAYHAWNNPEVEDKFGAFTQYDIEGAKAQLAEAGFKDIDGDGFVETPSGEQIDIEVIVPNGWTDWVNSSQIAVEG
LNAAGIKANVSTPESAIWTEKLIKGDYDMAINSVRVGATPFNQYLDSLHEINQAKSRFAASRYYNEELSDLLDAFTQTSD
TDKQMAIMSDVQEIVGEDMPLAYVFNNPRWYQYNTKRFEGFFNADNPVANPVVHKTNPARLIHLLNLRPVE

Sequences:

>Translated_551_residues
MKFTATAGLLASVALWTTAAQAEETVLSALPEQVTAWVENFNPFNQTTAAPSVMHFMYEPLIIFNALDGGKPIYRLATAF
EYSDDLSSITVTLRDGVQWSDGEAFTADDVVKSFDLALSDPALDSVGMAQMLSGVEKLDEMTVKFNLSTPSSQAMYQIVR
VPIVPEHVWSNVSDPVTFTNPDPVGSGPLTEIRRFTPQEYIQCRNNNYWDAESLKVDCMRFPQIANNDQALAAAANGELD
WMGSFLPDIDNTFVAKDPEHHSYWLPAGSLVAFYMNFEAKEAGDKEAVNNVAFRRAVSMAFDREAMVEIAGYGYPTINQY
PSGLGRAYHAWNNPEVEDKFGAFTQYDIEGAKAQLAEAGFKDIDGDGFVETPSGEQIDIEVIVPNGWTDWVNSSQIAVEG
LNAAGIKANVSTPESAIWTEKLIKGDYDMAINSVRVGATPFNQYLDSLHEINQAKSRFAASRYYNEELSDLLDAFTQTSD
TDKQMAIMSDVQEIVGEDMPLAYVFNNPRWYQYNTKRFEGFFNADNPVANPVVHKTNPARLIHLLNLRPVE
>Mature_551_residues
MKFTATAGLLASVALWTTAAQAEETVLSALPEQVTAWVENFNPFNQTTAAPSVMHFMYEPLIIFNALDGGKPIYRLATAF
EYSDDLSSITVTLRDGVQWSDGEAFTADDVVKSFDLALSDPALDSVGMAQMLSGVEKLDEMTVKFNLSTPSSQAMYQIVR
VPIVPEHVWSNVSDPVTFTNPDPVGSGPLTEIRRFTPQEYIQCRNNNYWDAESLKVDCMRFPQIANNDQALAAAANGELD
WMGSFLPDIDNTFVAKDPEHHSYWLPAGSLVAFYMNFEAKEAGDKEAVNNVAFRRAVSMAFDREAMVEIAGYGYPTINQY
PSGLGRAYHAWNNPEVEDKFGAFTQYDIEGAKAQLAEAGFKDIDGDGFVETPSGEQIDIEVIVPNGWTDWVNSSQIAVEG
LNAAGIKANVSTPESAIWTEKLIKGDYDMAINSVRVGATPFNQYLDSLHEINQAKSRFAASRYYNEELSDLLDAFTQTSD
TDKQMAIMSDVQEIVGEDMPLAYVFNNPRWYQYNTKRFEGFFNADNPVANPVVHKTNPARLIHLLNLRPVE

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=517, Percent_Identity=23.9845261121857, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1787052, Length=446, Percent_Identity=23.542600896861, Blast_Score=73, Evalue=6e-14,
Organism=Escherichia coli, GI87082063, Length=456, Percent_Identity=21.7105263157895, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61159; Mature: 61159

Theoretical pI: Translated: 4.17; Mature: 4.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTATAGLLASVALWTTAAQAEETVLSALPEQVTAWVENFNPFNQTTAAPSVMHFMYEP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCC
LIIFNALDGGKPIYRLATAFEYSDDLSSITVTLRDGVQWSDGEAFTADDVVKSFDLALSD
EEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEEECCCEECCCCCCCHHHHHHHHHHHCCC
PALDSVGMAQMLSGVEKLDEMTVKFNLSTPSSQAMYQIVRVPIVPEHVWSNVSDPVTFTN
CCCCCCCHHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHCCCCCHHHHCCCCCCEEECC
PDPVGSGPLTEIRRFTPQEYIQCRNNNYWDAESLKVDCMRFPQIANNDQALAAAANGELD
CCCCCCCCHHHHHHCCCHHHHHCCCCCCCCHHHCEEHHHHCCCCCCCCCEEEEECCCCHH
WMGSFLPDIDNTFVAKDPEHHSYWLPAGSLVAFYMNFEAKEAGDKEAVNNVAFRRAVSMA
HHHHHCCCCCCCEECCCCCCCEEECCCCCEEEEEECCCHHCCCCHHHHHHHHHHHHHHHH
FDREAMVEIAGYGYPTINQYPSGLGRAYHAWNNPEVEDKFGAFTQYDIEGAKAQLAEAGF
HCHHHHHHHHCCCCCCHHHCCCHHHHHHHCCCCCCCHHHHCCCEEECCCCHHHHHHHCCC
KDIDGDGFVETPSGEQIDIEVIVPNGWTDWVNSSQIAVEGLNAAGIKANVSTPESAIWTE
CCCCCCCCEECCCCCEEEEEEEECCCCHHHCCCCEEEEECCCCCCEEEECCCCCHHHHHH
KLIKGDYDMAINSVRVGATPFNQYLDSLHEINQAKSRFAASRYYNEELSDLLDAFTQTSD
HHHCCCHHHEECEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TDKQMAIMSDVQEIVGEDMPLAYVFNNPRWYQYNTKRFEGFFNADNPVANPVVHKTNPAR
CHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECHHHCCCEECCCCCCCCCCEECCCHHH
LIHLLNLRPVE
EEEEECCCCCC
>Mature Secondary Structure
MKFTATAGLLASVALWTTAAQAEETVLSALPEQVTAWVENFNPFNQTTAAPSVMHFMYEP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCC
LIIFNALDGGKPIYRLATAFEYSDDLSSITVTLRDGVQWSDGEAFTADDVVKSFDLALSD
EEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEEECCCEECCCCCCCHHHHHHHHHHHCCC
PALDSVGMAQMLSGVEKLDEMTVKFNLSTPSSQAMYQIVRVPIVPEHVWSNVSDPVTFTN
CCCCCCCHHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHCCCCCHHHHCCCCCCEEECC
PDPVGSGPLTEIRRFTPQEYIQCRNNNYWDAESLKVDCMRFPQIANNDQALAAAANGELD
CCCCCCCCHHHHHHCCCHHHHHCCCCCCCCHHHCEEHHHHCCCCCCCCCEEEEECCCCHH
WMGSFLPDIDNTFVAKDPEHHSYWLPAGSLVAFYMNFEAKEAGDKEAVNNVAFRRAVSMA
HHHHHCCCCCCCEECCCCCCCEEECCCCCEEEEEECCCHHCCCCHHHHHHHHHHHHHHHH
FDREAMVEIAGYGYPTINQYPSGLGRAYHAWNNPEVEDKFGAFTQYDIEGAKAQLAEAGF
HCHHHHHHHHCCCCCCHHHCCCHHHHHHHCCCCCCCHHHHCCCEEECCCCHHHHHHHCCC
KDIDGDGFVETPSGEQIDIEVIVPNGWTDWVNSSQIAVEGLNAAGIKANVSTPESAIWTE
CCCCCCCCEECCCCCEEEEEEEECCCCHHHCCCCEEEEECCCCCCEEEECCCCCHHHHHH
KLIKGDYDMAINSVRVGATPFNQYLDSLHEINQAKSRFAASRYYNEELSDLLDAFTQTSD
HHHCCCHHHEECEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TDKQMAIMSDVQEIVGEDMPLAYVFNNPRWYQYNTKRFEGFFNADNPVANPVVHKTNPAR
CHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECHHHCCCEECCCCCCCCCCEECCCHHH
LIHLLNLRPVE
EEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]