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The map label for this gene is mdeA [H]

Identifier: 99082577

GI number: 99082577

Start: 2881941

End: 2883143

Strand: Reverse

Name: mdeA [H]

Synonym: TM1040_2737

Alternate gene names: 99082577

Gene position: 2883143-2881941 (Counterclockwise)

Preceding gene: 99082579

Following gene: 99082576

Centisome position: 90.07

GC content: 62.76

Gene sequence:

>1203_bases
ATGTCCGAGACCAAAGGCTTTGCCACCCGCGCCATCCACCACGCCTATGACCCGATGCAGAACGAGGGCGCGCTGACGCC
GCCGCTGCATCTGACCTCCACCTTCGTGTTCGAAAATGCCGAAGCCGGCGGCGAGATGTTCGCAGGCGAGCGCGAGGGGC
ATATCTACAGCCGAATCTCGAACCCGACCTGTTCGCTTCTGGAGCAGCGTATCGCAACGCTAGAAGGGGCCGAAGCCGGT
CTGGCGCTGGCAAGCGGCATGGGCGCGGTGACGGCGATTCTCTGGACGCTCCTCAGCCCCGGTGACGAGGTGATCGTCGA
CAAGACGCTCTATGGGTGCACCTTTGCCTATATGCGCCATGGGCTTGCAAAATGGGGCGTGACCATCACCCACGTCGACA
TGACCGACCCCGCCGCCCTGAGCGCCGCCGTGAGCGAGAAGACCCGCGTCGTCTATTTCGAGACGCCCGCCAACCCCAAC
ATGCGCTTGGTCGACATCAAACGCGCCGCAGAGATTGCGCATTCCGTCGACGCGCGCGTTGTGGTCGACAACACCTATGC
GACGCCCTACCTCACGCGCCCGATCGAGCTTGGCGCGGATCTGGTGCTGCATTCGGCGACCAAATACCTTGGTGGTCACG
GCGATGTGGTCGCAGGGCTTGTGGCGGGCAGCGCCGAGCTTTTGACCGAAATCCGCCTTGTCGGCATGAAGGACATGACC
GGTGCGGTGATGGCGCCCTTCAATGCGATGCTGATCATGCGCGGCTTGAAAACACTGGCGCTACGGATGGACCGTCATTG
TGCCTCGGCGCAGACCGTCGCGGAGTGGCTAGAGGCCCAGCCCGGCGTCGCCAAGGTCTATTTCCCCGGTCTCAAGAGCT
CTGAACAGCACGAGATCGCCAAGGCGCAGATGGCGCAGCCCGGCGGCATGATTGCCTTTGAACTCGAGGGCGGCATGGAG
GCCGGGATCAGCCTGATGAACCGCCTGCAGATGATCCAGCGCGCGGTGTCGCTTGGCGATGCGGAGACGCTCATCCAGCA
CCCGGCTTCGATGACACATTCCACCTATACCCCCGAGGAACGCGCGGAACATGGCATCAGCGATGGCTTGGTGCGCTTGT
CCGTGGGGCTTGAAGAGGTTTCGGACATTCTCGATGATCTTCAGCAAGCTCTGTCGCCTCAGATGCGTCAGGCTGCCGAG
TGA

Upstream 100 bases:

>100_bases
GGCATAATCTGCCAATATCATGTCTCGCATTGGTAGTTTTTGCAATCTCTCGCCCTGTGTGGAGGCGCATCATGGCAGGC
AAATGAGGAAGGAGACCCCC

Downstream 100 bases:

>100_bases
AAGGCGGGTCCATGTCCCAGAACATTGAACATCTGAGAACGGTGGAGCAGCGACTGTTGTGGCTGTCCCATTGGATGATC
CACAACGCCAACCATTTGCG

Product: methionine gamma-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MSETKGFATRAIHHAYDPMQNEGALTPPLHLTSTFVFENAEAGGEMFAGEREGHIYSRISNPTCSLLEQRIATLEGAEAG
LALASGMGAVTAILWTLLSPGDEVIVDKTLYGCTFAYMRHGLAKWGVTITHVDMTDPAALSAAVSEKTRVVYFETPANPN
MRLVDIKRAAEIAHSVDARVVVDNTYATPYLTRPIELGADLVLHSATKYLGGHGDVVAGLVAGSAELLTEIRLVGMKDMT
GAVMAPFNAMLIMRGLKTLALRMDRHCASAQTVAEWLEAQPGVAKVYFPGLKSSEQHEIAKAQMAQPGGMIAFELEGGME
AGISLMNRLQMIQRAVSLGDAETLIQHPASMTHSTYTPEERAEHGISDGLVRLSVGLEEVSDILDDLQQALSPQMRQAAE

Sequences:

>Translated_400_residues
MSETKGFATRAIHHAYDPMQNEGALTPPLHLTSTFVFENAEAGGEMFAGEREGHIYSRISNPTCSLLEQRIATLEGAEAG
LALASGMGAVTAILWTLLSPGDEVIVDKTLYGCTFAYMRHGLAKWGVTITHVDMTDPAALSAAVSEKTRVVYFETPANPN
MRLVDIKRAAEIAHSVDARVVVDNTYATPYLTRPIELGADLVLHSATKYLGGHGDVVAGLVAGSAELLTEIRLVGMKDMT
GAVMAPFNAMLIMRGLKTLALRMDRHCASAQTVAEWLEAQPGVAKVYFPGLKSSEQHEIAKAQMAQPGGMIAFELEGGME
AGISLMNRLQMIQRAVSLGDAETLIQHPASMTHSTYTPEERAEHGISDGLVRLSVGLEEVSDILDDLQQALSPQMRQAAE
>Mature_399_residues
SETKGFATRAIHHAYDPMQNEGALTPPLHLTSTFVFENAEAGGEMFAGEREGHIYSRISNPTCSLLEQRIATLEGAEAGL
ALASGMGAVTAILWTLLSPGDEVIVDKTLYGCTFAYMRHGLAKWGVTITHVDMTDPAALSAAVSEKTRVVYFETPANPNM
RLVDIKRAAEIAHSVDARVVVDNTYATPYLTRPIELGADLVLHSATKYLGGHGDVVAGLVAGSAELLTEIRLVGMKDMTG
AVMAPFNAMLIMRGLKTLALRMDRHCASAQTVAEWLEAQPGVAKVYFPGLKSSEQHEIAKAQMAQPGGMIAFELEGGMEA
GISLMNRLQMIQRAVSLGDAETLIQHPASMTHSTYTPEERAEHGISDGLVRLSVGLEEVSDILDDLQQALSPQMRQAAE

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=387, Percent_Identity=42.3772609819121, Blast_Score=276, Evalue=3e-74,
Organism=Homo sapiens, GI299473759, Length=387, Percent_Identity=39.0180878552972, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI25453487, Length=386, Percent_Identity=37.5647668393782, Blast_Score=215, Evalue=5e-56,
Organism=Escherichia coli, GI1790375, Length=390, Percent_Identity=38.4615384615385, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI1789383, Length=375, Percent_Identity=28.5333333333333, Blast_Score=151, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI71980708, Length=379, Percent_Identity=38.7862796833773, Blast_Score=265, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI17538055, Length=395, Percent_Identity=38.9873417721519, Blast_Score=263, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI17560056, Length=395, Percent_Identity=36.4556962025316, Blast_Score=252, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6319307, Length=400, Percent_Identity=38, Blast_Score=243, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6323333, Length=410, Percent_Identity=32.4390243902439, Blast_Score=221, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6321254, Length=400, Percent_Identity=32, Blast_Score=172, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6321170, Length=325, Percent_Identity=27.0769230769231, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321904, Length=398, Percent_Identity=26.6331658291457, Blast_Score=107, Evalue=3e-24,
Organism=Drosophila melanogaster, GI19922554, Length=392, Percent_Identity=40.0510204081633, Blast_Score=283, Evalue=2e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 43017; Mature: 42886

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETKGFATRAIHHAYDPMQNEGALTPPLHLTSTFVFENAEAGGEMFAGEREGHIYSRIS
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHEEEEECCCCCCCCCCCCCCCCCHHHCC
NPTCSLLEQRIATLEGAEAGLALASGMGAVTAILWTLLSPGDEVIVDKTLYGCTFAYMRH
CCHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHC
GLAKWGVTITHVDMTDPAALSAAVSEKTRVVYFETPANPNMRLVDIKRAAEIAHSVDARV
CHHHHCEEEEEECCCCCHHHHHHHCCCEEEEEEECCCCCCEEEEEHHHHHHHHHCCCCEE
VVDNTYATPYLTRPIELGADLVLHSATKYLGGHGDVVAGLVAGSAELLTEIRLVGMKDMT
EEECCCCCCCCCCHHHHCHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHCCCCCC
GAVMAPFNAMLIMRGLKTLALRMDRHCASAQTVAEWLEAQPGVAKVYFPGLKSSEQHEIA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHH
KAQMAQPGGMIAFELEGGMEAGISLMNRLQMIQRAVSLGDAETLIQHPASMTHSTYTPEE
HHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHCCCCCCCHH
RAEHGISDGLVRLSVGLEEVSDILDDLQQALSPQMRQAAE
HHHCCCCCCEEEEECCHHHHHHHHHHHHHHHCHHHHHHCC
>Mature Secondary Structure 
SETKGFATRAIHHAYDPMQNEGALTPPLHLTSTFVFENAEAGGEMFAGEREGHIYSRIS
CCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHEEEEECCCCCCCCCCCCCCCCCHHHCC
NPTCSLLEQRIATLEGAEAGLALASGMGAVTAILWTLLSPGDEVIVDKTLYGCTFAYMRH
CCHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHC
GLAKWGVTITHVDMTDPAALSAAVSEKTRVVYFETPANPNMRLVDIKRAAEIAHSVDARV
CHHHHCEEEEEECCCCCHHHHHHHCCCEEEEEEECCCCCCEEEEEHHHHHHHHHCCCCEE
VVDNTYATPYLTRPIELGADLVLHSATKYLGGHGDVVAGLVAGSAELLTEIRLVGMKDMT
EEECCCCCCCCCCHHHHCHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHCCCCCC
GAVMAPFNAMLIMRGLKTLALRMDRHCASAQTVAEWLEAQPGVAKVYFPGLKSSEQHEIA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHH
KAQMAQPGGMIAFELEGGMEAGISLMNRLQMIQRAVSLGDAETLIQHPASMTHSTYTPEE
HHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHCCCCCCCHH
RAEHGISDGLVRLSVGLEEVSDILDDLQQALSPQMRQAAE
HHHCCCCCCEEEEECCHHHHHHHHHHHHHHHCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]