Click here to switch to the map view.

The map label for this gene is lepB [H]

Identifier: 99082400

GI number: 99082400

Start: 2703577

End: 2704413

Strand: Reverse

Name: lepB [H]

Synonym: TM1040_2560

Alternate gene names: 99082400

Gene position: 2704413-2703577 (Counterclockwise)

Preceding gene: 99082401

Following gene: 99082399

Centisome position: 84.49

GC content: 56.87

Gene sequence:

>837_bases
ATGGCGTCGAAAACCAAAAGCGGGAACTCAATCGTCGAGACGATCAAGACCATTGTCTACGCCCTGTTGATTGCCGGTGT
CTTCCGCACCCTGTTCTTTCAACCGTTCTGGATTCCGTCTGGCTCGATGAAGGAGACGCTGCTGATCGGAGATTTCCTCT
TCGTCAACAAGATGGTCTATGGCTATTCCTACGCCTCCTGCCCGAATATCCGCCTGAACTCCATCGGGATCAATATCGAC
GCCAAGGACATCTGCGGCTTTGTAGACGGGGACAACACCCGTATTTTCGGGGGTGAGCCAGAGCGCGGCGACGTTGTCGT
ATTCCGTCATCCCGTAAATGGAACCGACTACATCAAGCGCCTTGTGGGGCTCCCGGGTGACAAATTGCAGATGAGAGATG
GCGTGCTGCACATCAATGGCGCGGCAGTTGGTCTGATGGATGATGGCGAGTTCGAAGAAGCAATGGAGCGCCAGGGGCCT
CAGGGCAGGTTCCCGCGCTGTGAAAACGACCCCGTTGGTCAGGGCGGCGTCTGCAAGAAATCGCGTCAGATCGAGACGCT
TCCGGGCGGCACCTCGCATGCGATCATCAACATCGGCAATCAGGCCAGCGACCACACCAAGGTCTATCATGTGCCAGACG
GTCATTTCTTCTTTATGGGGGATAACCGTGACAACTCCACCGACAGCCGGGTGTCCAAGGCAGTGGGCGGTGTTGGCTAC
GTCCCCTTCGAGAATCTGATCGGGCGTGCAGATCGCATCATGTTCTCTTCGGCGGGGCGCTCGATGCTGTTCTTCTGGAC
CTGGCGCGGGGATCGCTTCTTTAAGGGCATCACCTGA

Upstream 100 bases:

>100_bases
CATCGCGCCACAAACGCGCGCTCTGATCGCCACCGCTTGACTTGCGGCGGACAGCCCCGCATGTAGCCCCGGAGCAGTCC
ACTAATGACCGGAGAGCCTG

Downstream 100 bases:

>100_bases
CAACGGACCCTATCGGGATCATCCATGTCGGCCGTAGGTCTTTTGCATCTGCGGCCGTTTTGCTGCGCGCAATATCAAGA
GTTGCGATTGGCGCAAGGCC

Product: signal peptidase I

Products: NA

Alternate protein names: SPase I; Leader peptidase I [H]

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MASKTKSGNSIVETIKTIVYALLIAGVFRTLFFQPFWIPSGSMKETLLIGDFLFVNKMVYGYSYASCPNIRLNSIGINID
AKDICGFVDGDNTRIFGGEPERGDVVVFRHPVNGTDYIKRLVGLPGDKLQMRDGVLHINGAAVGLMDDGEFEEAMERQGP
QGRFPRCENDPVGQGGVCKKSRQIETLPGGTSHAIINIGNQASDHTKVYHVPDGHFFFMGDNRDNSTDSRVSKAVGGVGY
VPFENLIGRADRIMFSSAGRSMLFFWTWRGDRFFKGIT

Sequences:

>Translated_278_residues
MASKTKSGNSIVETIKTIVYALLIAGVFRTLFFQPFWIPSGSMKETLLIGDFLFVNKMVYGYSYASCPNIRLNSIGINID
AKDICGFVDGDNTRIFGGEPERGDVVVFRHPVNGTDYIKRLVGLPGDKLQMRDGVLHINGAAVGLMDDGEFEEAMERQGP
QGRFPRCENDPVGQGGVCKKSRQIETLPGGTSHAIINIGNQASDHTKVYHVPDGHFFFMGDNRDNSTDSRVSKAVGGVGY
VPFENLIGRADRIMFSSAGRSMLFFWTWRGDRFFKGIT
>Mature_277_residues
ASKTKSGNSIVETIKTIVYALLIAGVFRTLFFQPFWIPSGSMKETLLIGDFLFVNKMVYGYSYASCPNIRLNSIGINIDA
KDICGFVDGDNTRIFGGEPERGDVVVFRHPVNGTDYIKRLVGLPGDKLQMRDGVLHINGAAVGLMDDGEFEEAMERQGPQ
GRFPRCENDPVGQGGVCKKSRQIETLPGGTSHAIINIGNQASDHTKVYHVPDGHFFFMGDNRDNSTDSRVSKAVGGVGYV
PFENLIGRADRIMFSSAGRSMLFFWTWRGDRFFKGIT

Specific function: Unknown

COG id: COG0681

COG function: function code U; Signal peptidase I

Gene ontology:

Cell location: Cell membrane; Single-pass type II membrane protein (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S26 family [H]

Homologues:

Organism=Escherichia coli, GI1788921, Length=275, Percent_Identity=33.0909090909091, Blast_Score=111, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000223
- InterPro:   IPR019758
- InterPro:   IPR019757
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056 [H]

Pfam domain/function: PF00717 Peptidase_S24 [H]

EC number: =3.4.21.89 [H]

Molecular weight: Translated: 30694; Mature: 30563

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS00760 SPASE_I_2 ; PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASKTKSGNSIVETIKTIVYALLIAGVFRTLFFQPFWIPSGSMKETLLIGDFLFVNKMVY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCEEEEHHHHHHHHHHH
GYSYASCPNIRLNSIGINIDAKDICGFVDGDNTRIFGGEPERGDVVVFRHPVNGTDYIKR
CCCCCCCCCEEEEEEEEEEEHHHHEEEECCCCEEEECCCCCCCCEEEEECCCCHHHHHHH
LVGLPGDKLQMRDGVLHINGAAVGLMDDGEFEEAMERQGPQGRFPRCENDPVGQGGVCKK
HHCCCCCCEEECCCEEEECCEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
SRQIETLPGGTSHAIINIGNQASDHTKVYHVPDGHFFFMGDNRDNSTDSRVSKAVGGVGY
CCCEEECCCCCCEEEEEECCCCCCCCEEEECCCCEEEEEECCCCCCHHHHHHHHHCCCCC
VPFENLIGRADRIMFSSAGRSMLFFWTWRGDRFFKGIT
CCHHHHHCHHHHEEEECCCCEEEEEEEECCCEEECCCC
>Mature Secondary Structure 
ASKTKSGNSIVETIKTIVYALLIAGVFRTLFFQPFWIPSGSMKETLLIGDFLFVNKMVY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCEEEEHHHHHHHHHHH
GYSYASCPNIRLNSIGINIDAKDICGFVDGDNTRIFGGEPERGDVVVFRHPVNGTDYIKR
CCCCCCCCCEEEEEEEEEEEHHHHEEEECCCCEEEECCCCCCCCEEEEECCCCHHHHHHH
LVGLPGDKLQMRDGVLHINGAAVGLMDDGEFEEAMERQGPQGRFPRCENDPVGQGGVCKK
HHCCCCCCEEECCCEEEECCEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
SRQIETLPGGTSHAIINIGNQASDHTKVYHVPDGHFFFMGDNRDNSTDSRVSKAVGGVGY
CCCEEECCCCCCEEEEEECCCCCCCCEEEECCCCEEEEEECCCCCCHHHHHHHHHCCCCC
VPFENLIGRADRIMFSSAGRSMLFFWTWRGDRFFKGIT
CCHHHHHCHHHHEEEECCCCEEEEEEEECCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]