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The map label for this gene is gspE [C]

Identifier: 99082189

GI number: 99082189

Start: 2470318

End: 2471775

Strand: Direct

Name: gspE [C]

Synonym: TM1040_2349

Alternate gene names: 99082189

Gene position: 2470318-2471775 (Clockwise)

Preceding gene: 99082188

Following gene: 99082190

Centisome position: 77.17

GC content: 58.78

Gene sequence:

>1458_bases
ATGTTTTCCAAGTACAAAAAGCAATCCTCTGCGCCTGCTGCCCCCGCAGCGGCCCCTGCCGCGGCTCCAAAACCTGCCGC
CAGCCCCAGCGCACCTGCCGCCGGGGTCAAGGCCGTTCTGCGCCGTCCCGACCAGCGCAACGCAGCTGCCGCCGCACCGC
AGGACCGTGAGCGCAAGCGCAAGGAACGGCTGAGCGAGATCAAAATTGATCTGCACAGAGAGCTTTTGGAGAACCTCAAC
CTCGCCGCGCTTGAAAAAGCCTCTGAATCTGAGCTGCGCAGCGAGATCGCAGCCATCTCCAATGAGGTGCTTCAGGAAAA
AAGCATCGTTTTGAACCGCGAAGACCGTCAGCAGCTCATGAAAGAGCTGTATGACGAGGTCACGGGTCTTGGCCCGCTCG
AAGCGCTTTTGAAGGATGATACCGTTTCCGATATTCTGGTGAACGGCCCCCAGCAGATCTTTATCGAACGTGCAGGTAAG
CTCGAACTGAGCGACATTACCTTCAAGGATGAAAAGCACCTGATGCGGATCATCGACAAGATCGTCTCGGCTGTGGGGCG
TCGAGTTGATGAATCAAATCCCTATGTGGATGCCCGTCTCCAGGATGGGTCGCGCTTTAATGCCATGGTGCCGCCGATTG
CGGTAGATGGCTCACTGGTGTCGATCCGGAAATTCAAAAAGGACAAGTTACAGATCGACGATCTGGTAAACTTTGGTGCC
TTCACCGAAGAAATGGCCGCCTATCTTCAGGCTGCTGTCTCGACCCGTCTGAATGTCATCGTGTCCGGCGGGACGGGCTC
TGGTAAGACGACCACGCTCAACGCGCTGTCGAGCTTTATCGACAACTCCGAGCGCATCCTGACCATTGAGGATACGGCGG
AACTGCAGTTGCAACAGACCCATGTGGGACGCATGGAAAGCCGCCCGCCCAACGTCGAGGGCAAGGGAGAGGTCAGCCCG
CGCGACTGTCTGAAAAACGCGCTGCGGATGCGCCCGGATCGCATCATCGTCGGCGAAACGCGTGGCGCCGAAGTGATCGA
CATGCTGCAGGCGATGAACACGGGCCACGACGGCTCGATGACCACGATCCACGCAAACTCTGCCCGTGACGGGATCTCGC
GTCTCGAGAACATGATCGCCATGGCCGGGATCGAGATGCCGATCAAGGCTGTGCGCAGCCAGATTGCCTCGGCTGTGAAC
CTGATCGTACAGGCCTCGCGCCTTCAGGATGGTTCGCGCCGGATGACCTCCATCACCGAGATCACCGGGATGGAAGGCGA
TGTTATCTCCATGCAGGAAATCTTTCGCTTCCAGCGCGTGGGCCTTACCCCCGACAACAAGATCATCGGCCATTTTACCG
CGACCGGCGTGCGCAGCCATTTCTCGGAACGCTTCCGCCTCTGGGGCTATGACCTGCCGGCGTCGATCTACGAGCCAACC
GTGATGGAGAGCCGCTGA

Upstream 100 bases:

>100_bases
GCAAGGAAATCGCCAAGCTGGCGAAGAACCTGCATGAGCTGGGACAGGCAGAGGCGGAAGCCGCCTAAGTCGCAACGCGA
CAGCACGGGAAGGTATCGAA

Downstream 100 bases:

>100_bases
TGGAATTCGGGATTGAACCGCTTATCTACGGGCTGATCTTCATCGGCGTCCTGGTGCTGGTCGAAGGCATCTATCTGGTT
GCCTTTGGCAAATCCATCAG

Product: type II secretion system protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MFSKYKKQSSAPAAPAAAPAAAPKPAASPSAPAAGVKAVLRRPDQRNAAAAAPQDRERKRKERLSEIKIDLHRELLENLN
LAALEKASESELRSEIAAISNEVLQEKSIVLNREDRQQLMKELYDEVTGLGPLEALLKDDTVSDILVNGPQQIFIERAGK
LELSDITFKDEKHLMRIIDKIVSAVGRRVDESNPYVDARLQDGSRFNAMVPPIAVDGSLVSIRKFKKDKLQIDDLVNFGA
FTEEMAAYLQAAVSTRLNVIVSGGTGSGKTTTLNALSSFIDNSERILTIEDTAELQLQQTHVGRMESRPPNVEGKGEVSP
RDCLKNALRMRPDRIIVGETRGAEVIDMLQAMNTGHDGSMTTIHANSARDGISRLENMIAMAGIEMPIKAVRSQIASAVN
LIVQASRLQDGSRRMTSITEITGMEGDVISMQEIFRFQRVGLTPDNKIIGHFTATGVRSHFSERFRLWGYDLPASIYEPT
VMESR

Sequences:

>Translated_485_residues
MFSKYKKQSSAPAAPAAAPAAAPKPAASPSAPAAGVKAVLRRPDQRNAAAAAPQDRERKRKERLSEIKIDLHRELLENLN
LAALEKASESELRSEIAAISNEVLQEKSIVLNREDRQQLMKELYDEVTGLGPLEALLKDDTVSDILVNGPQQIFIERAGK
LELSDITFKDEKHLMRIIDKIVSAVGRRVDESNPYVDARLQDGSRFNAMVPPIAVDGSLVSIRKFKKDKLQIDDLVNFGA
FTEEMAAYLQAAVSTRLNVIVSGGTGSGKTTTLNALSSFIDNSERILTIEDTAELQLQQTHVGRMESRPPNVEGKGEVSP
RDCLKNALRMRPDRIIVGETRGAEVIDMLQAMNTGHDGSMTTIHANSARDGISRLENMIAMAGIEMPIKAVRSQIASAVN
LIVQASRLQDGSRRMTSITEITGMEGDVISMQEIFRFQRVGLTPDNKIIGHFTATGVRSHFSERFRLWGYDLPASIYEPT
VMESR
>Mature_485_residues
MFSKYKKQSSAPAAPAAAPAAAPKPAASPSAPAAGVKAVLRRPDQRNAAAAAPQDRERKRKERLSEIKIDLHRELLENLN
LAALEKASESELRSEIAAISNEVLQEKSIVLNREDRQQLMKELYDEVTGLGPLEALLKDDTVSDILVNGPQQIFIERAGK
LELSDITFKDEKHLMRIIDKIVSAVGRRVDESNPYVDARLQDGSRFNAMVPPIAVDGSLVSIRKFKKDKLQIDDLVNFGA
FTEEMAAYLQAAVSTRLNVIVSGGTGSGKTTTLNALSSFIDNSERILTIEDTAELQLQQTHVGRMESRPPNVEGKGEVSP
RDCLKNALRMRPDRIIVGETRGAEVIDMLQAMNTGHDGSMTTIHANSARDGISRLENMIAMAGIEMPIKAVRSQIASAVN
LIVQASRLQDGSRRMTSITEITGMEGDVISMQEIFRFQRVGLTPDNKIIGHFTATGVRSHFSERFRLWGYDLPASIYEPT
VMESR

Specific function: Involved In A General Secretion Pathway (Gsp) For The Export Of Proteins (By Similarity). [C]

COG id: COG4962

COG function: function code U; Flp pilus assembly protein, ATPase CpaF

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=122, Percent_Identity=36.8852459016393, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022399
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 53357; Mature: 53357

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00227 TUBULIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSKYKKQSSAPAAPAAAPAAAPKPAASPSAPAAGVKAVLRRPDQRNAAAAAPQDRERKR
CCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHH
KERLSEIKIDLHRELLENLNLAALEKASESELRSEIAAISNEVLQEKSIVLNREDRQQLM
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
KELYDEVTGLGPLEALLKDDTVSDILVNGPQQIFIERAGKLELSDITFKDEKHLMRIIDK
HHHHHHHCCCCHHHHHHCCCCHHHHHHCCHHHHHHHHCCCEEEECCCCCHHHHHHHHHHH
IVSAVGRRVDESNPYVDARLQDGSRFNAMVPPIAVDGSLVSIRKFKKDKLQIDDLVNFGA
HHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCH
FTEEMAAYLQAAVSTRLNVIVSGGTGSGKTTTLNALSSFIDNSERILTIEDTAELQLQQT
HHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHH
HVGRMESRPPNVEGKGEVSPRDCLKNALRMRPDRIIVGETRGAEVIDMLQAMNTGHDGSM
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCE
TTIHANSARDGISRLENMIAMAGIEMPIKAVRSQIASAVNLIVQASRLQDGSRRMTSITE
EEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
ITGMEGDVISMQEIFRFQRVGLTPDNKIIGHFTATGVRSHFSERFRLWGYDLPASIYEPT
HCCCCCCEECHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCHHHCCCC
VMESR
CCCCC
>Mature Secondary Structure
MFSKYKKQSSAPAAPAAAPAAAPKPAASPSAPAAGVKAVLRRPDQRNAAAAAPQDRERKR
CCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHH
KERLSEIKIDLHRELLENLNLAALEKASESELRSEIAAISNEVLQEKSIVLNREDRQQLM
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
KELYDEVTGLGPLEALLKDDTVSDILVNGPQQIFIERAGKLELSDITFKDEKHLMRIIDK
HHHHHHHCCCCHHHHHHCCCCHHHHHHCCHHHHHHHHCCCEEEECCCCCHHHHHHHHHHH
IVSAVGRRVDESNPYVDARLQDGSRFNAMVPPIAVDGSLVSIRKFKKDKLQIDDLVNFGA
HHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCH
FTEEMAAYLQAAVSTRLNVIVSGGTGSGKTTTLNALSSFIDNSERILTIEDTAELQLQQT
HHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHH
HVGRMESRPPNVEGKGEVSPRDCLKNALRMRPDRIIVGETRGAEVIDMLQAMNTGHDGSM
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCE
TTIHANSARDGISRLENMIAMAGIEMPIKAVRSQIASAVNLIVQASRLQDGSRRMTSITE
EEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
ITGMEGDVISMQEIFRFQRVGLTPDNKIIGHFTATGVRSHFSERFRLWGYDLPASIYEPT
HCCCCCCEECHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCHHHCCCC
VMESR
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036; 9634230 [H]