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The map label for this gene is leuA [H]

Identifier: 99082122

GI number: 99082122

Start: 2400415

End: 2401998

Strand: Direct

Name: leuA [H]

Synonym: TM1040_2282

Alternate gene names: 99082122

Gene position: 2400415-2401998 (Clockwise)

Preceding gene: 99082120

Following gene: 99082124

Centisome position: 74.99

GC content: 61.3

Gene sequence:

>1584_bases
ATGACCAGCAAGACCGACACCGTGAACCAGCAGGATCGCGTTCTCATTTTTGACACCACGTTGCGCGATGGCGAGCAGAG
CCCCGGCGCGACCATGACCCATGATGAAAAGCTCGAGATTGCCGAGCTTCTGGACGATATGGGTGTCGACATCATCGAGG
CCGGTTTCCCGATTGCCTCGGAGGGCGATTTCAAGGCCGTGAGCGAGATCGCGGAACGCTCCAAGAACTCTGTGATCTGT
GGCCTGGCGCGTGCGAATTTCAAGGACATCGACCGCTGCGCCGAAGCCGTGCGCAAGGCTGCGAAGCCCCGCATCCACAC
GTTTATCGGCACGTCGCCGCTGCACCGCGCCATTCCCAACCTTACCATGGATGAGATGGCCGAGCGCATCCACGACACCG
TGACCCATGCGCGCAACCTCGTGGACAATGTGCAATGGTCGCCGATGGATGCCACGCGCACCGAATGGGATTACCTCTGC
CGGGTCATCGAGATCGCGATCAAGGCGGGGGCGACTACCATCAACATCCCTGACACGGTTGGCTATACCGCGCCGCGCGA
GAGCGCCGACCTGATCCGGCGCCTGATTGAAACCGTGCCCGGCGCTGACGAGATCATCTTTGCGACCCATTGCCATAACG
ACCTTGGCATGGCGACCGCGAACTCTTTGGCCGCAGTCGAGGCGGGCGCGCGCCAGATCGAATGCACGATCAACGGCCTT
GGCGAGCGTGCGGGCAATACCGCGCTCGAAGAGGTGGTGATGGCAATGCGCGTGCGCAACGACATCATGCCGTTCCAGAC
CGGGATCGACACCAAGAAGATCATGCATATCTCGCGCCGCGTCGCCACGGTTTCGGGCTTTAACGTGCAGTTCAACAAGG
CGATCGTCGGCAAGAATGCCTTTGCCCACGAAAGCGGCATCCACCAGGATGGCATGCTCAAGAACAAGGAAACCTTCGAG
ATCATGCGCCCCGAAGACGTGGGCCTTGCTGGAACGTCGCTCCCCTTGGGCAAACACTCCGGCCGCGCCGCGCTGCGCGA
CAAGCTCTCGAGCCTCGGGTTTGAAATCGGAGACAACCAGCTCAAGGATGTATTCGTGCGCTTCAAGGAGCTCGCGGACC
GCAAGAAAGAGGTGTTCGACGACGACATCATCGCGCTCATGCGCACCAGCGGCGACGAGGACGATCACCTGAAGCTGGTG
TCGATGAAAGTGGTCTGCGGCACCGGTGGTCCGGCGGAATCCACCGTCGAGATGGAAGTTGACGGCAAGGACGTGACGGA
AACCGCCGAGGGCGACGGACCGGTGGATGCCACATTCAAGGCGATCCGCAAGATCCACCCCAACGCCGCGCATCTGCAGC
TTTATCAGGTGCACGCCGTGACCGAGGGCACGGACGCGCAGGCAACCGTCTCTGTGCGCCTTGAAGAAGACGGCGTGATC
GCCACCGGCGACAGCGCCAACACCGATACGGTTGTGGCCTCTGCGATGGCATACATTGACGCGCTCAACCGCCTGATCGT
GCGCCGCGACAAGATGGGCGAAGGCGCGGATTCGCGCGAGATTTCCTATAAAGACGTGGACTGA

Upstream 100 bases:

>100_bases
CGCCCGCTCAGAGGATGTGCATGCGCGCCATGGATTGGACCCTACCCCCCGAACAACATGCCTGACGTCCCCGGGGATCA
GAGCAAGAAAGACATTTGAC

Downstream 100 bases:

>100_bases
CACACGTCACATCAAAGATAAAAAAGGGCGCCGTCGGGCGTCCTTTTTTGTTACTGTGCATGACGTTGCTGCAGGCGCGG
ACTACTGCCGCTGCAACAGC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MTSKTDTVNQQDRVLIFDTTLRDGEQSPGATMTHDEKLEIAELLDDMGVDIIEAGFPIASEGDFKAVSEIAERSKNSVIC
GLARANFKDIDRCAEAVRKAAKPRIHTFIGTSPLHRAIPNLTMDEMAERIHDTVTHARNLVDNVQWSPMDATRTEWDYLC
RVIEIAIKAGATTINIPDTVGYTAPRESADLIRRLIETVPGADEIIFATHCHNDLGMATANSLAAVEAGARQIECTINGL
GERAGNTALEEVVMAMRVRNDIMPFQTGIDTKKIMHISRRVATVSGFNVQFNKAIVGKNAFAHESGIHQDGMLKNKETFE
IMRPEDVGLAGTSLPLGKHSGRAALRDKLSSLGFEIGDNQLKDVFVRFKELADRKKEVFDDDIIALMRTSGDEDDHLKLV
SMKVVCGTGGPAESTVEMEVDGKDVTETAEGDGPVDATFKAIRKIHPNAAHLQLYQVHAVTEGTDAQATVSVRLEEDGVI
ATGDSANTDTVVASAMAYIDALNRLIVRRDKMGEGADSREISYKDVD

Sequences:

>Translated_527_residues
MTSKTDTVNQQDRVLIFDTTLRDGEQSPGATMTHDEKLEIAELLDDMGVDIIEAGFPIASEGDFKAVSEIAERSKNSVIC
GLARANFKDIDRCAEAVRKAAKPRIHTFIGTSPLHRAIPNLTMDEMAERIHDTVTHARNLVDNVQWSPMDATRTEWDYLC
RVIEIAIKAGATTINIPDTVGYTAPRESADLIRRLIETVPGADEIIFATHCHNDLGMATANSLAAVEAGARQIECTINGL
GERAGNTALEEVVMAMRVRNDIMPFQTGIDTKKIMHISRRVATVSGFNVQFNKAIVGKNAFAHESGIHQDGMLKNKETFE
IMRPEDVGLAGTSLPLGKHSGRAALRDKLSSLGFEIGDNQLKDVFVRFKELADRKKEVFDDDIIALMRTSGDEDDHLKLV
SMKVVCGTGGPAESTVEMEVDGKDVTETAEGDGPVDATFKAIRKIHPNAAHLQLYQVHAVTEGTDAQATVSVRLEEDGVI
ATGDSANTDTVVASAMAYIDALNRLIVRRDKMGEGADSREISYKDVD
>Mature_526_residues
TSKTDTVNQQDRVLIFDTTLRDGEQSPGATMTHDEKLEIAELLDDMGVDIIEAGFPIASEGDFKAVSEIAERSKNSVICG
LARANFKDIDRCAEAVRKAAKPRIHTFIGTSPLHRAIPNLTMDEMAERIHDTVTHARNLVDNVQWSPMDATRTEWDYLCR
VIEIAIKAGATTINIPDTVGYTAPRESADLIRRLIETVPGADEIIFATHCHNDLGMATANSLAAVEAGARQIECTINGLG
ERAGNTALEEVVMAMRVRNDIMPFQTGIDTKKIMHISRRVATVSGFNVQFNKAIVGKNAFAHESGIHQDGMLKNKETFEI
MRPEDVGLAGTSLPLGKHSGRAALRDKLSSLGFEIGDNQLKDVFVRFKELADRKKEVFDDDIIALMRTSGDEDDHLKLVS
MKVVCGTGGPAESTVEMEVDGKDVTETAEGDGPVDATFKAIRKIHPNAAHLQLYQVHAVTEGTDAQATVSVRLEEDGVIA
TGDSANTDTVVASAMAYIDALNRLIVRRDKMGEGADSREISYKDVD

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=496, Percent_Identity=46.1693548387097, Blast_Score=421, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6320019, Length=379, Percent_Identity=30.8707124010554, Blast_Score=160, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6320071, Length=379, Percent_Identity=30.8707124010554, Blast_Score=159, Evalue=7e-40,
Organism=Saccharomyces cerevisiae, GI6324682, Length=506, Percent_Identity=24.3083003952569, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324225, Length=498, Percent_Identity=25.1004016064257, Blast_Score=108, Evalue=2e-24,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 57489; Mature: 57357

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSKTDTVNQQDRVLIFDTTLRDGEQSPGATMTHDEKLEIAELLDDMGVDIIEAGFPIAS
CCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEECCCCCCCC
EGDFKAVSEIAERSKNSVICGLARANFKDIDRCAEAVRKAAKPRIHTFIGTSPLHRAIPN
CCCHHHHHHHHHCCCCCEEEEEHHCCHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHCCC
LTMDEMAERIHDTVTHARNLVDNVQWSPMDATRTEWDYLCRVIEIAIKAGATTINIPDTV
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCC
GYTAPRESADLIRRLIETVPGADEIIFATHCHNDLGMATANSLAAVEAGARQIECTINGL
CCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEEEECC
GERAGNTALEEVVMAMRVRNDIMPFQTGIDTKKIMHISRRVATVSGFNVQFNKAIVGKNA
CCCCCCHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHEEECCCEEECCEEECCCC
FAHESGIHQDGMLKNKETFEIMRPEDVGLAGTSLPLGKHSGRAALRDKLSSLGFEIGDNQ
HHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHH
LKDVFVRFKELADRKKEVFDDDIIALMRTSGDEDDHLKLVSMKVVCGTGGPAESTVEMEV
HHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCCCEEEEEE
DGKDVTETAEGDGPVDATFKAIRKIHPNAAHLQLYQVHAVTEGTDAQATVSVRLEEDGVI
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHEEEEEEEEECCCCCCEEEEEEEEECCCEE
ATGDSANTDTVVASAMAYIDALNRLIVRRDKMGEGADSREISYKDVD
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TSKTDTVNQQDRVLIFDTTLRDGEQSPGATMTHDEKLEIAELLDDMGVDIIEAGFPIAS
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEECCCCCCCC
EGDFKAVSEIAERSKNSVICGLARANFKDIDRCAEAVRKAAKPRIHTFIGTSPLHRAIPN
CCCHHHHHHHHHCCCCCEEEEEHHCCHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHCCC
LTMDEMAERIHDTVTHARNLVDNVQWSPMDATRTEWDYLCRVIEIAIKAGATTINIPDTV
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCC
GYTAPRESADLIRRLIETVPGADEIIFATHCHNDLGMATANSLAAVEAGARQIECTINGL
CCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEEEECC
GERAGNTALEEVVMAMRVRNDIMPFQTGIDTKKIMHISRRVATVSGFNVQFNKAIVGKNA
CCCCCCHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHEEECCCEEECCEEECCCC
FAHESGIHQDGMLKNKETFEIMRPEDVGLAGTSLPLGKHSGRAALRDKLSSLGFEIGDNQ
HHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHH
LKDVFVRFKELADRKKEVFDDDIIALMRTSGDEDDHLKLVSMKVVCGTGGPAESTVEMEV
HHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCCCEEEEEE
DGKDVTETAEGDGPVDATFKAIRKIHPNAAHLQLYQVHAVTEGTDAQATVSVRLEEDGVI
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHEEEEEEEEECCCCCCEEEEEEEEECCCEE
ATGDSANTDTVVASAMAYIDALNRLIVRRDKMGEGADSREISYKDVD
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA