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The map label for this gene is yogA [H]

Identifier: 99082070

GI number: 99082070

Start: 2344893

End: 2345891

Strand: Direct

Name: yogA [H]

Synonym: TM1040_2230

Alternate gene names: 99082070

Gene position: 2344893-2345891 (Clockwise)

Preceding gene: 99082068

Following gene: 99082087

Centisome position: 73.26

GC content: 63.76

Gene sequence:

>999_bases
ATGAAAGCCATAGTTCACACCGCACGCGGCACCGCGCGGGACACCTTGGTCCTGACCGAAACAGAGACCCCGACCCCCGG
CCCCGGAGAGGTGCTGGTCGCGATGCGGGCCTCTGGGGTCAACCCATCGGATGTCAAGCTGCGCTCTGGCGCGCAGGGGC
CGATGGTGGCCGAGCAGGTCTTGGTTCACAACGATGGCGCAGGCGTAATCGAGGCCGTCGGCGACGGTGTCGGACCGGCC
CGGATCGGGCGTCGGGTCTGGCTCTACAATGTGAACCGCTCTGCGGATGGGCTAGGTCAGGGCCCGCTTGGAACAGCGGC
GGAATTCGCAATTGTGCCAGCACATCTTGCGGTGGATCTGCCGGATGCGGCCAGCTTCGAGGCCGGCGCCTGCATCGGCG
TGCCCGCAATGACAGCGCATCGCGCAGTGATGTGGGCCGGATCGGTCGCGGGCAAGACCCTGTTGATCACCGGCGGCGCA
GGCGCAGTCGGGCACAGTGCCATCCAGATTGCCAAGGCCAAAGGCGCGCGCGTCATCGCCACCGTCTCAAGCGACGCGAA
GGCTGAGATCGCCCGCGCAGCAGGCGCCGATGAGGTGCTCAACTACAAGAGCACGCCTTTGGTGGATCAGCTGATCGAAC
TGGTGGGCGCAGGCGGTATTGACCATGTCGTCGACGTGGATATCGCCGCGCATATGGAGATCTACCCGCAGATCCTAAAG
CTCAACGGTTCGGTCGGGGCCTATGCCTCCGCCAGTAACCTCACGCCCGCCCTGCCCTTTTACCCGCTGGCCTTTCGCAA
CATTCAGATCCAGCCAGTTTTTGTCTACTCGATGAGTGACAGCGCCAAAGCAGAGGCGATTGCAGACATCAACGCGCTCC
TGTCCGCGGGTCAGCTGACGCCGCGCATTGACCGCGTCTTTGCGCTTGAGGAGATCGTTGACGCACATGAGGCCGTGGAG
GCAGGCACCCTGGTTGGAAACGCGATCCTGAAGATCTGA

Upstream 100 bases:

>100_bases
CCGGCGCAGCCCCTGCTTAATGCGCGCTGTCCCCCTTGCGTTTGCAGTGCATTTTGGGGCTCATACGGTGCGAAATACCA
AACCAGAAACAGGAGCGAGC

Downstream 100 bases:

>100_bases
GTGCCGAACACAAAACAAAAAGGGCCCCGCAGATATGGGGCCCTTTTTGTGAATTGAAGCACACTGGTACTCAGCTGATA
TGCGCGGTGGCCTCGATTTC

Product: zinc-binding alcohol dehydrogenase

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MKAIVHTARGTARDTLVLTETETPTPGPGEVLVAMRASGVNPSDVKLRSGAQGPMVAEQVLVHNDGAGVIEAVGDGVGPA
RIGRRVWLYNVNRSADGLGQGPLGTAAEFAIVPAHLAVDLPDAASFEAGACIGVPAMTAHRAVMWAGSVAGKTLLITGGA
GAVGHSAIQIAKAKGARVIATVSSDAKAEIARAAGADEVLNYKSTPLVDQLIELVGAGGIDHVVDVDIAAHMEIYPQILK
LNGSVGAYASASNLTPALPFYPLAFRNIQIQPVFVYSMSDSAKAEAIADINALLSAGQLTPRIDRVFALEEIVDAHEAVE
AGTLVGNAILKI

Sequences:

>Translated_332_residues
MKAIVHTARGTARDTLVLTETETPTPGPGEVLVAMRASGVNPSDVKLRSGAQGPMVAEQVLVHNDGAGVIEAVGDGVGPA
RIGRRVWLYNVNRSADGLGQGPLGTAAEFAIVPAHLAVDLPDAASFEAGACIGVPAMTAHRAVMWAGSVAGKTLLITGGA
GAVGHSAIQIAKAKGARVIATVSSDAKAEIARAAGADEVLNYKSTPLVDQLIELVGAGGIDHVVDVDIAAHMEIYPQILK
LNGSVGAYASASNLTPALPFYPLAFRNIQIQPVFVYSMSDSAKAEAIADINALLSAGQLTPRIDRVFALEEIVDAHEAVE
AGTLVGNAILKI
>Mature_332_residues
MKAIVHTARGTARDTLVLTETETPTPGPGEVLVAMRASGVNPSDVKLRSGAQGPMVAEQVLVHNDGAGVIEAVGDGVGPA
RIGRRVWLYNVNRSADGLGQGPLGTAAEFAIVPAHLAVDLPDAASFEAGACIGVPAMTAHRAVMWAGSVAGKTLLITGGA
GAVGHSAIQIAKAKGARVIATVSSDAKAEIARAAGADEVLNYKSTPLVDQLIELVGAGGIDHVVDVDIAAHMEIYPQILK
LNGSVGAYASASNLTPALPFYPLAFRNIQIQPVFVYSMSDSAKAEAIADINALLSAGQLTPRIDRVFALEEIVDAHEAVE
AGTLVGNAILKI

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI194239674, Length=301, Percent_Identity=29.9003322259136, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI13236495, Length=301, Percent_Identity=29.9003322259136, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI194239676, Length=301, Percent_Identity=28.2392026578073, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI22538446, Length=339, Percent_Identity=25.0737463126844, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI22538444, Length=339, Percent_Identity=25.0737463126844, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI47519420, Length=349, Percent_Identity=25.7879656160458, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI28557745, Length=356, Percent_Identity=26.123595505618, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI18379349, Length=320, Percent_Identity=26.25, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI226059159, Length=226, Percent_Identity=24.3362831858407, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI226059133, Length=226, Percent_Identity=24.3362831858407, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI24308257, Length=360, Percent_Identity=23.3333333333333, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI197927207, Length=174, Percent_Identity=27.0114942528736, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1790485, Length=315, Percent_Identity=27.6190476190476, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1787863, Length=245, Percent_Identity=29.7959183673469, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87082125, Length=238, Percent_Identity=28.9915966386555, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17507255, Length=327, Percent_Identity=28.4403669724771, Blast_Score=110, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17562876, Length=214, Percent_Identity=31.3084112149533, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71988145, Length=326, Percent_Identity=26.3803680981595, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17562878, Length=208, Percent_Identity=29.3269230769231, Blast_Score=65, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6319520, Length=330, Percent_Identity=23.3333333333333, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319621, Length=248, Percent_Identity=26.2096774193548, Blast_Score=67, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6324486, Length=338, Percent_Identity=24.5562130177515, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323729, Length=326, Percent_Identity=22.6993865030675, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6319955, Length=322, Percent_Identity=26.7080745341615, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323961, Length=327, Percent_Identity=23.8532110091743, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 34024; Mature: 34024

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAIVHTARGTARDTLVLTETETPTPGPGEVLVAMRASGVNPSDVKLRSGAQGPMVAEQV
CCCEEECCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCCEEEECCCCCCHHHHEE
LVHNDGAGVIEAVGDGVGPARIGRRVWLYNVNRSADGLGQGPLGTAAEFAIVPAHLAVDL
EEECCCCCHHHHHCCCCCHHHHCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEEEEEEEC
PDAASFEAGACIGVPAMTAHRAVMWAGSVAGKTLLITGGAGAVGHSAIQIAKAKGARVIA
CCCCCCCCCCEECCCCHHHHHEEEEECCCCCCEEEEECCCCCCCHHHEEEEECCCCEEEE
TVSSDAKAEIARAAGADEVLNYKSTPLVDQLIELVGAGGIDHVVDVDIAAHMEIYPQILK
EECCCHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEEHHHHHHHHCEEHEE
LNGSVGAYASASNLTPALPFYPLAFRNIQIQPVFVYSMSDSAKAEAIADINALLSAGQLT
ECCCCCCEECCCCCCCCCCCCCEEECCEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCC
PRIDRVFALEEIVDAHEAVEAGTLVGNAILKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure
MKAIVHTARGTARDTLVLTETETPTPGPGEVLVAMRASGVNPSDVKLRSGAQGPMVAEQV
CCCEEECCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCCEEEECCCCCCHHHHEE
LVHNDGAGVIEAVGDGVGPARIGRRVWLYNVNRSADGLGQGPLGTAAEFAIVPAHLAVDL
EEECCCCCHHHHHCCCCCHHHHCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEEEEEEEC
PDAASFEAGACIGVPAMTAHRAVMWAGSVAGKTLLITGGAGAVGHSAIQIAKAKGARVIA
CCCCCCCCCCEECCCCHHHHHEEEEECCCCCCEEEEECCCCCCCHHHEEEEECCCCEEEE
TVSSDAKAEIARAAGADEVLNYKSTPLVDQLIELVGAGGIDHVVDVDIAAHMEIYPQILK
EECCCHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEEHHHHHHHHCEEHEE
LNGSVGAYASASNLTPALPFYPLAFRNIQIQPVFVYSMSDSAKAEAIADINALLSAGQLT
ECCCCCCEECCCCCCCCCCCCCEEECCEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCC
PRIDRVFALEEIVDAHEAVEAGTLVGNAILKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]