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The map label for this gene is cobJ [H]

Identifier: 99082054

GI number: 99082054

Start: 2326739

End: 2327560

Strand: Direct

Name: cobJ [H]

Synonym: TM1040_2214

Alternate gene names: 99082054

Gene position: 2326739-2327560 (Clockwise)

Preceding gene: 99082053

Following gene: 99082056

Centisome position: 72.69

GC content: 64.23

Gene sequence:

>822_bases
ATGAATGACACCCCAAAAGGCTGGGTGGTGATTGCAGGCCTTGGCCCCGGCGCCGAGAGCATGGTGATCCCCGAGGTCCA
GGACGTGCTGGCCGAGGCCACCGATGTGGTCGGCTACATCCCTTATGTAAAACGCATCCCGCCGCGCGCGGGGCTGACGC
TTCATGCCAGCGACAACCGGGTTGAGGTCGACCGCGCCACCCAAGCGCTAGAAATGGCCGCAGCGGGACAACGCGTGGTG
GTGGTCTCTTCCGGCGATCCGGGTGTCTTTGCTATGGCATCAGCGGTGTTTGAGGCGCTCGAAGAAGGCGCGAAGACGCA
GCCTGCGTGGCTCAACCTCGAGATCCGCGTGCTGCCCGGCATCACCGCCATGCTCGCTGCGGCCGCTGCGATTGGCGCAC
CTCTGGGGCATGATTTTGCCGCCATCAACCTCAGCGACAACCTGAAACCCTGGGAGCTGATCGAGCGCCGGTTGCAGCTT
GTGGGCGAAGCGGGGCTGGCGATGGCGTTTTACAACCCGCGTTCCAAATCCCGCCCGCATCAGTTCGAGCGCGCACTAGA
GGTGCTGCGCGCAGCCTGCGGGGACGACACGCTCATCAGCTTTGCCCGCGATGTCACAAAACCAGGGCAAGAGATCCTGA
CCGTGCCGCTCCGCGATGCCCGCCCCGAGATGGCGGATATGCGCACTGTCGTGATCGTCGGCAATCGCGACACCCGGCGC
GTCGGCTCTTATGTCTACACCCCGCGGTTTGCACCCGGACACCAGCCTGGACACCAGCCCGCTTATCACACAGCCGCCAA
CACCGCCACAAAGGACGCTTAA

Upstream 100 bases:

>100_bases
GCGGCACCATGCCGGCCCAGACGGTGCAAAAGCTCACCGATGTCGACGGCGAGGTGCCGTATTTCTCGATCGTGATCCTG
CACGGAGAAGGACGCCGCCC

Downstream 100 bases:

>100_bases
GCCCCGTGGTCAAGCCAGCGCATCACCTCTGCCACCGAGCCCAGCACCTCGCGTGCGGGCCCCTCGGGCCGGTCGATCAT
AATGACCGGCAACGCAAGAC

Product: precorrin-3 methyltransferase

Products: NA

Alternate protein names: Precorrin-3 methylase; Precorrin-3 methyltransferase [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MNDTPKGWVVIAGLGPGAESMVIPEVQDVLAEATDVVGYIPYVKRIPPRAGLTLHASDNRVEVDRATQALEMAAAGQRVV
VVSSGDPGVFAMASAVFEALEEGAKTQPAWLNLEIRVLPGITAMLAAAAAIGAPLGHDFAAINLSDNLKPWELIERRLQL
VGEAGLAMAFYNPRSKSRPHQFERALEVLRAACGDDTLISFARDVTKPGQEILTVPLRDARPEMADMRTVVIVGNRDTRR
VGSYVYTPRFAPGHQPGHQPAYHTAANTATKDA

Sequences:

>Translated_273_residues
MNDTPKGWVVIAGLGPGAESMVIPEVQDVLAEATDVVGYIPYVKRIPPRAGLTLHASDNRVEVDRATQALEMAAAGQRVV
VVSSGDPGVFAMASAVFEALEEGAKTQPAWLNLEIRVLPGITAMLAAAAAIGAPLGHDFAAINLSDNLKPWELIERRLQL
VGEAGLAMAFYNPRSKSRPHQFERALEVLRAACGDDTLISFARDVTKPGQEILTVPLRDARPEMADMRTVVIVGNRDTRR
VGSYVYTPRFAPGHQPGHQPAYHTAANTATKDA
>Mature_273_residues
MNDTPKGWVVIAGLGPGAESMVIPEVQDVLAEATDVVGYIPYVKRIPPRAGLTLHASDNRVEVDRATQALEMAAAGQRVV
VVSSGDPGVFAMASAVFEALEEGAKTQPAWLNLEIRVLPGITAMLAAAAAIGAPLGHDFAAINLSDNLKPWELIERRLQL
VGEAGLAMAFYNPRSKSRPHQFERALEVLRAACGDDTLISFARDVTKPGQEILTVPLRDARPEMADMRTVVIVGNRDTRR
VGSYVYTPRFAPGHQPGHQPAYHTAANTATKDA

Specific function: Methyltransferase that catalyzes the methylation of C-17 in precorrin-3B to form precorrin-4 [H]

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006363 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.131 [H]

Molecular weight: Translated: 29308; Mature: 29308

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDTPKGWVVIAGLGPGAESMVIPEVQDVLAEATDVVGYIPYVKRIPPRAGLTLHASDNR
CCCCCCCEEEEEECCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCE
VEVDRATQALEMAAAGQRVVVVSSGDPGVFAMASAVFEALEEGAKTQPAWLNLEIRVLPG
EEHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCC
ITAMLAAAAAIGAPLGHDFAAINLSDNLKPWELIERRLQLVGEAGLAMAFYNPRSKSRPH
HHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCH
QFERALEVLRAACGDDTLISFARDVTKPGQEILTVPLRDARPEMADMRTVVIVGNRDTRR
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCHHH
VGSYVYTPRFAPGHQPGHQPAYHTAANTATKDA
HCCEEECCCCCCCCCCCCCCCHHHHCCCCCCCC
>Mature Secondary Structure
MNDTPKGWVVIAGLGPGAESMVIPEVQDVLAEATDVVGYIPYVKRIPPRAGLTLHASDNR
CCCCCCCEEEEEECCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCE
VEVDRATQALEMAAAGQRVVVVSSGDPGVFAMASAVFEALEEGAKTQPAWLNLEIRVLPG
EEHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCC
ITAMLAAAAAIGAPLGHDFAAINLSDNLKPWELIERRLQLVGEAGLAMAFYNPRSKSRPH
HHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCH
QFERALEVLRAACGDDTLISFARDVTKPGQEILTVPLRDARPEMADMRTVVIVGNRDTRR
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCHHH
VGSYVYTPRFAPGHQPGHQPAYHTAANTATKDA
HCCEEECCCCCCCCCCCCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2211521; 8226690 [H]