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The map label for this gene is rpoD [H]

Identifier: 99081981

GI number: 99081981

Start: 2245649

End: 2247643

Strand: Reverse

Name: rpoD [H]

Synonym: TM1040_2141

Alternate gene names: 99081981

Gene position: 2247643-2245649 (Counterclockwise)

Preceding gene: 99081982

Following gene: 99081980

Centisome position: 70.22

GC content: 58.6

Gene sequence:

>1995_bases
ATGGCCGCCAAAGATACTGACGACCGCAAGCCTGACGATCAGGACGCCGAAATTTCGCTCGATATGAGCCAGGCGCAAGT
CAAGAAGATGATCGCCGAAGCCCGCGAGAAGGGCTACATCACCTACGATCAGCTCAATCAGGTTTTGCCGCCGGATCAGG
TCTCCTCCGAACAGATCGAAGACGTGATGTCTATGCTGTCGGAAATGGGCATCAACATCATCGAAGATGAAGAGGCCGAG
GAAGAAGAGAACAAAGGCACCACCGAACTGGTGACCACGGAATCCAACCGTGAAGTCGCAGTGGCTGGCGCTGCGACTGA
GAAGCTCGACCGGACCGACGATCCGGTCCGCATGTACCTGCGCGAAATGGGCAGCGTCGAGCTGTTGTCGCGCGAGGGCG
AGATCGCGATTGCAAAGCGCATCGAGGCCGGGCGCAACACCATGATCCTCGGGCTCTGCGAAAGCCCGCTGACATTTCAG
GCGATCACCATCTGGCATGACGAACTCCTCTCCGAGGATATTCTTCTGCGCGATGTGATTGATCTTGAGGCGACCTTTGG
CAATCAGATGGACGAGGACGGTGACGTCGCCGAGCCGGTTGTCGATCCCTCTGCAGTGTCTGGCGCTGCAAAGCCAGAAA
AAGACTCCGGGCCCGAGCTTGATGCCGATGGCAACCCGATCCTCAACAACGACGACGATGACGACGACGACGATGATCAG
GCCAACATGTCCCTTGCGGCCATGGAAGCGGCGCTCAAGGATCGGGTTCTGGAAACGCTCGAGCGGATTTCCAGTGACTT
TGCGATGCTGTCGGAAATGCAGGATCTGCGGATCTCTGCGACGCTCAATGAGGATGGGTCCTTTTCGGCTGATGACGAAG
CCAAGTACCAGCAGCTGCGCTCTGAAATCGTGGAACTGGTGAACGGTCTTCACCTGCACAACAACCGCATCGAGGCGCTG
ATCGACCAGCTTTATGGTATCAACCGCCGCGTCATGCAGATCGATAGCGCCATGGTCAAACTCGCTGACCAGGCCCGTAT
CAACCGCAAGGAATTCGTCGAAGCTTACCGTGGTCGCGAACTCGACCCCAACTGGCTTTCCGAGATGAGCGAAAAGCCCG
GTCGGGGCTGGCAGATGTTCATCGAGCGCTCGACCGAGAAGGTCGAAGAGCTGCGCGCCGACATGGCACAGGTCGGTCAG
TACGTCGGTCTGGACATCTCGGAATTCCGCCGCATCGTGCAGCAGGTGCAAAAAGGTGAAAAAGAGGCCCGTCAGGCCAA
GAAGGAAATGGTCGAGGCCAACCTGCGCCTCGTGATCTCAATCGCCAAGAAATACACCAACCGGGGCCTGCAGTTCCTCG
ACCTCATTCAGGAAGGCAACATCGGCCTGATGAAGGCGGTCGACAAGTTCGAATACCGTCGCGGCTATAAGTTCTCGACC
TATGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCGATCGCCGATCAAGCGCGCACCATCCGTATCCCGGTGCATAT
GATCGAGACCATCAACAAGCTGGTCCGCACCGGCCGTCAGATGCTCCACGAAATCGGCCGCGAGCCGACGCCGGAAGAGC
TGGCAGAAAAGCTGCAGATGCCGCTCGAGAAGGTCCGCAAGGTGATGAAGATCGCCAAGGAGCCCATTTCGCTCGAGACT
CCGATCGGGGACGAGGAAGACAGCCAGCTGGGCGATTTCATCGAGGACAAGAATGCCGTGCTGCCCCTGGACAGCGCCAT
TCAGGAAAACCTCAAGGAAACCACCACGCGGGTTCTGGCCTCGCTCACCCCGCGCGAGGAGCGCGTGCTGCGGATGCGGT
TTGGTATCGGCATGAACACCGATCACACGCTCGAAGAGGTGGGCCAGCAGTTCAGCGTGACCCGCGAACGGATCCGTCAG
ATCGAGGCCAAGGCGCTCAGGAAGCTCAAGCATCCGAGCCGCTCTCGCAAGCTGCGCAGCTTCCTCGATCAGTAA

Upstream 100 bases:

>100_bases
AAGCCTATAGAAAAATTGGGTAAACGGGGTGACGAATCACGAGAGCACTCTAAAGATTCGCTCCACTCGAATCGCCCGCA
GCCCCTTAGAGGAGCAATGA

Downstream 100 bases:

>100_bases
AAAGTGTTAACGTAAAGACGCCCCCGGAAGGGGGCGTTTTTTGTACGACCCTCTGAAAAAAATGGGCTGGTTGAGCCGCT
GCCTTTTGCGTCCACTCTGG

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-70 [H]

Number of amino acids: Translated: 664; Mature: 663

Protein sequence:

>664_residues
MAAKDTDDRKPDDQDAEISLDMSQAQVKKMIAEAREKGYITYDQLNQVLPPDQVSSEQIEDVMSMLSEMGINIIEDEEAE
EEENKGTTELVTTESNREVAVAGAATEKLDRTDDPVRMYLREMGSVELLSREGEIAIAKRIEAGRNTMILGLCESPLTFQ
AITIWHDELLSEDILLRDVIDLEATFGNQMDEDGDVAEPVVDPSAVSGAAKPEKDSGPELDADGNPILNNDDDDDDDDDQ
ANMSLAAMEAALKDRVLETLERISSDFAMLSEMQDLRISATLNEDGSFSADDEAKYQQLRSEIVELVNGLHLHNNRIEAL
IDQLYGINRRVMQIDSAMVKLADQARINRKEFVEAYRGRELDPNWLSEMSEKPGRGWQMFIERSTEKVEELRADMAQVGQ
YVGLDISEFRRIVQQVQKGEKEARQAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFST
YATWWIRQAITRSIADQARTIRIPVHMIETINKLVRTGRQMLHEIGREPTPEELAEKLQMPLEKVRKVMKIAKEPISLET
PIGDEEDSQLGDFIEDKNAVLPLDSAIQENLKETTTRVLASLTPREERVLRMRFGIGMNTDHTLEEVGQQFSVTRERIRQ
IEAKALRKLKHPSRSRKLRSFLDQ

Sequences:

>Translated_664_residues
MAAKDTDDRKPDDQDAEISLDMSQAQVKKMIAEAREKGYITYDQLNQVLPPDQVSSEQIEDVMSMLSEMGINIIEDEEAE
EEENKGTTELVTTESNREVAVAGAATEKLDRTDDPVRMYLREMGSVELLSREGEIAIAKRIEAGRNTMILGLCESPLTFQ
AITIWHDELLSEDILLRDVIDLEATFGNQMDEDGDVAEPVVDPSAVSGAAKPEKDSGPELDADGNPILNNDDDDDDDDDQ
ANMSLAAMEAALKDRVLETLERISSDFAMLSEMQDLRISATLNEDGSFSADDEAKYQQLRSEIVELVNGLHLHNNRIEAL
IDQLYGINRRVMQIDSAMVKLADQARINRKEFVEAYRGRELDPNWLSEMSEKPGRGWQMFIERSTEKVEELRADMAQVGQ
YVGLDISEFRRIVQQVQKGEKEARQAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFST
YATWWIRQAITRSIADQARTIRIPVHMIETINKLVRTGRQMLHEIGREPTPEELAEKLQMPLEKVRKVMKIAKEPISLET
PIGDEEDSQLGDFIEDKNAVLPLDSAIQENLKETTTRVLASLTPREERVLRMRFGIGMNTDHTLEEVGQQFSVTRERIRQ
IEAKALRKLKHPSRSRKLRSFLDQ
>Mature_663_residues
AAKDTDDRKPDDQDAEISLDMSQAQVKKMIAEAREKGYITYDQLNQVLPPDQVSSEQIEDVMSMLSEMGINIIEDEEAEE
EENKGTTELVTTESNREVAVAGAATEKLDRTDDPVRMYLREMGSVELLSREGEIAIAKRIEAGRNTMILGLCESPLTFQA
ITIWHDELLSEDILLRDVIDLEATFGNQMDEDGDVAEPVVDPSAVSGAAKPEKDSGPELDADGNPILNNDDDDDDDDDQA
NMSLAAMEAALKDRVLETLERISSDFAMLSEMQDLRISATLNEDGSFSADDEAKYQQLRSEIVELVNGLHLHNNRIEALI
DQLYGINRRVMQIDSAMVKLADQARINRKEFVEAYRGRELDPNWLSEMSEKPGRGWQMFIERSTEKVEELRADMAQVGQY
VGLDISEFRRIVQQVQKGEKEARQAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTY
ATWWIRQAITRSIADQARTIRIPVHMIETINKLVRTGRQMLHEIGREPTPEELAEKLQMPLEKVRKVMKIAKEPISLETP
IGDEEDSQLGDFIEDKNAVLPLDSAIQENLKETTTRVLASLTPREERVLRMRFGIGMNTDHTLEEVGQQFSVTRERIRQI
EAKALRKLKHPSRSRKLRSFLDQ

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=643, Percent_Identity=46.9673405909798, Blast_Score=522, Evalue=1e-149,
Organism=Escherichia coli, GI1789098, Length=225, Percent_Identity=42.6666666666667, Blast_Score=216, Evalue=4e-57,
Organism=Escherichia coli, GI1789871, Length=243, Percent_Identity=27.5720164609054, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=27.2277227722772, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007631
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04546 Sigma70_ner; PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 75492; Mature: 75361

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKDTDDRKPDDQDAEISLDMSQAQVKKMIAEAREKGYITYDQLNQVLPPDQVSSEQIE
CCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCEEHHHHHCCCCCCCCCHHHHH
DVMSMLSEMGINIIEDEEAEEEENKGTTELVTTESNREVAVAGAATEKLDRTDDPVRMYL
HHHHHHHHCCCEEECCCHHHHHHCCCCCEEEEECCCCEEEEECCHHHHHCCCCCHHHHHH
REMGSVELLSREGEIAIAKRIEAGRNTMILGLCESPLTFQAITIWHDELLSEDILLRDVI
HHCCCHHHHCCCCCCHHHHHHHCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHH
DLEATFGNQMDEDGDVAEPVVDPSAVSGAAKPEKDSGPELDADGNPILNNDDDDDDDDDQ
HHHHHHCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
ANMSLAAMEAALKDRVLETLERISSDFAMLSEMQDLRISATLNEDGSFSADDEAKYQQLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHHH
SEIVELVNGLHLHNNRIEALIDQLYGINRRVMQIDSAMVKLADQARINRKEFVEAYRGRE
HHHHHHHHCHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCC
LDPNWLSEMSEKPGRGWQMFIERSTEKVEELRADMAQVGQYVGLDISEFRRIVQQVQKGE
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
KEARQAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFST
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHH
YATWWIRQAITRSIADQARTIRIPVHMIETINKLVRTGRQMLHEIGREPTPEELAEKLQM
HHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
PLEKVRKVMKIAKEPISLETPIGDEEDSQLGDFIEDKNAVLPLDSAIQENLKETTTRVLA
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
SLTPREERVLRMRFGIGMNTDHTLEEVGQQFSVTRERIRQIEAKALRKLKHPSRSRKLRS
HCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
FLDQ
HHCC
>Mature Secondary Structure 
AAKDTDDRKPDDQDAEISLDMSQAQVKKMIAEAREKGYITYDQLNQVLPPDQVSSEQIE
CCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCEEHHHHHCCCCCCCCCHHHHH
DVMSMLSEMGINIIEDEEAEEEENKGTTELVTTESNREVAVAGAATEKLDRTDDPVRMYL
HHHHHHHHCCCEEECCCHHHHHHCCCCCEEEEECCCCEEEEECCHHHHHCCCCCHHHHHH
REMGSVELLSREGEIAIAKRIEAGRNTMILGLCESPLTFQAITIWHDELLSEDILLRDVI
HHCCCHHHHCCCCCCHHHHHHHCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHH
DLEATFGNQMDEDGDVAEPVVDPSAVSGAAKPEKDSGPELDADGNPILNNDDDDDDDDDQ
HHHHHHCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
ANMSLAAMEAALKDRVLETLERISSDFAMLSEMQDLRISATLNEDGSFSADDEAKYQQLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHHH
SEIVELVNGLHLHNNRIEALIDQLYGINRRVMQIDSAMVKLADQARINRKEFVEAYRGRE
HHHHHHHHCHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCC
LDPNWLSEMSEKPGRGWQMFIERSTEKVEELRADMAQVGQYVGLDISEFRRIVQQVQKGE
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
KEARQAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFST
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHH
YATWWIRQAITRSIADQARTIRIPVHMIETINKLVRTGRQMLHEIGREPTPEELAEKLQM
HHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
PLEKVRKVMKIAKEPISLETPIGDEEDSQLGDFIEDKNAVLPLDSAIQENLKETTTRVLA
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
SLTPREERVLRMRFGIGMNTDHTLEEVGQQFSVTRERIRQIEAKALRKLKHPSRSRKLRS
HCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
FLDQ
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA