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The map label for this gene is dnaG [H]

Identifier: 99081982

GI number: 99081982

Start: 2247809

End: 2249818

Strand: Reverse

Name: dnaG [H]

Synonym: TM1040_2142

Alternate gene names: 99081982

Gene position: 2249818-2247809 (Counterclockwise)

Preceding gene: 99081983

Following gene: 99081981

Centisome position: 70.29

GC content: 61.94

Gene sequence:

>2010_bases
ATGTCGCTGCCCCCCGGTTTCCTTGATGAATTGCGCACCCGCATCAGCCTGTCTGACGTTGTCGGGCGCAAGGTCATGTG
GGATCAGCGCAAAAGCCAGCCGGGCAAGGGCGACATGTGGTCTCCGTGCCCCTTTCATCATGAAAAAACCGCTTCTTTTC
ATGTGGATGATCGCAAAGGATTTTACTACTGCTTTGGCTGTCACGCCAAAGGGGATGCGCTGAAATTCGTGCAGGAGACA
GAGAATGTCTCCTTTATGGAGGCGGTAGAGATCCTTGCGGGCGAGGCCGGGCTGGAGATGCCCAAGCGCGACCCCAAAGC
GGCTGAGAAACAGGACCGTCGCACGCAGCTTGCTGAGGTGATGGAACAGGCGGTGAAATACTTCCGCCTGCAACTCAAGA
CTCAAGCCGCGAGTGAGGCGCGCGCCTACCTCGCGCGTCGGGGGCTTGATGCGGCAGCGCTGGACCGCTGGGAGATCGGC
TTTGCCCCCGATGGCTGGCAGAACCTTTGGGATGCGCTCAAAGGCAAGGACATCGCGGATGATCTGATCCTCGGTGCCGG
GCTGGCCAAACCCTCGAACAAGGGCAAGAAGCCCTATGATGTGTTTCGCAACCGGATCATGTTCCCGATCCGGAATGCGC
GGGGCCGGGCGATCGCCTTTGGCGGCCGGGCGATGGATCCCAATGACAACGCCAAATACCTGAACTCGCCGGAAACCGAG
CTGTTTGATAAGGGGCGCAATCTCTATAACCACGGGCCCGCGCGCGCGGCCTCGGGACGGGGCAGCACCTTGATCGTGGC
CGAAGGCTACATGGATGTGATCGCGCTGTCCGAGGCGGGGTTCCAGTCGGCGGTGGCGCCCTTGGGTACAGCCGTCACCG
ACAACCAGCTGCATCTTTTATGGCGCATGTCGGACGAGCCGGTGATTGCGCTGGATGGGGATACGGCTGGTCTGCGCGCG
GCGATGCGGCTGATCGATATGGCGCTGCCTCTGCTCGAGGCCGGGAAGTCCTTGCGCTTTGCCATCATGCCCGAGGGCAA
GGACCCAGATGATCTTATTCGCGCCGAGGGACCAGAGGCGGTGCAGGCGGTGCTGGATCAGGCGATTCCGATGGTACAGC
TGCTCTGGCGCCGCGAGACCGAAGGGCGGGTGTTTGACAGCCCCGAGCGCAAAGCGGCCCTGGACAAGTCCCTGCGTGAA
AAGATCAAATTGATCCGTGACCCTTCTATTCGCCTGCATTACGCCAATGATATCAAGGAGCTTCGCTTTCAGCTCTTTCG
CGGTCAGCGTGGCGGTGGCGGGCAGGGGGGCACCGGCTATGGCCAGCGGTACGGCGGTTTCAAACCGGGCTCCCGCATGC
AGGGTGGATTTCGCGACGGCGCCCGCATGCCTTGGGGTGCGCCGCCCCCGCCCCGCAGCACCACACGGGCCTCGATGGTG
GCCTCCTCCGAGGAACAGGACTTTGCCCGCGTGCGCGAGGCGGTGATCCTCGCCACAGCAATCACCACGCCCACGGTGAT
CCCGGAATTCGAAATCAATCTTGAGCGCATGCAGTGTCAGGACATGGAACATGCCCACCTGCGCGATCTGGTTCTGCGCT
ATGGGATCGAGGCCCCGGAGCGGTTGCGCGACGAAATTGCCTATGCTCTTGGGCCGGACGCTCTTGAAAACCTGCTGTCG
CTGCGCCATGTGGCTATTAGCCCCTGCGTGCGCAAACCTGGCGACCTGGATATGGCCAGCCTGACCCTGGCCGAGGAATT
CGCAAAACTCGAGAGCATCGCGGGGTTGAATGCTGAGCTGGCGGAAGCCGAGGAAGATCTGGATGGTCTGGCGGATGAGG
CCCTGACCTGGCGTTTGCGTCAGGCGGCAGAGGCCCGCAACCGGGCTGTGCGCAGCGAAAATGAGGATAAGGCAACTTAC
GACGTGGGGGAGAACGGGGCGCGGCTCAACCGGGATGAGCGCGATGCCTTCGGAGACCTGCTCAAGCGAATCGGTTTCAA
CAGCAAATGA

Upstream 100 bases:

>100_bases
TCCAGGAAATGAAGAAGAACCTCTCCGGAGGCTAACGCCCGCATCTGTGCAGCCCGGCCTGCCGGGCTGCATTTTCCTCT
GAGCACTGGACCTTTTGTGA

Downstream 100 bases:

>100_bases
CGCAATGAGCACGATTCGCGGGCTCAACTGTGTCCGCGACGCCGCGATGTGACGTTTTGCTAAAGAAGCCTATAGAAAAA
TTGGGTAAACGGGGTGACGA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 669; Mature: 668

Protein sequence:

>669_residues
MSLPPGFLDELRTRISLSDVVGRKVMWDQRKSQPGKGDMWSPCPFHHEKTASFHVDDRKGFYYCFGCHAKGDALKFVQET
ENVSFMEAVEILAGEAGLEMPKRDPKAAEKQDRRTQLAEVMEQAVKYFRLQLKTQAASEARAYLARRGLDAAALDRWEIG
FAPDGWQNLWDALKGKDIADDLILGAGLAKPSNKGKKPYDVFRNRIMFPIRNARGRAIAFGGRAMDPNDNAKYLNSPETE
LFDKGRNLYNHGPARAASGRGSTLIVAEGYMDVIALSEAGFQSAVAPLGTAVTDNQLHLLWRMSDEPVIALDGDTAGLRA
AMRLIDMALPLLEAGKSLRFAIMPEGKDPDDLIRAEGPEAVQAVLDQAIPMVQLLWRRETEGRVFDSPERKAALDKSLRE
KIKLIRDPSIRLHYANDIKELRFQLFRGQRGGGGQGGTGYGQRYGGFKPGSRMQGGFRDGARMPWGAPPPPRSTTRASMV
ASSEEQDFARVREAVILATAITTPTVIPEFEINLERMQCQDMEHAHLRDLVLRYGIEAPERLRDEIAYALGPDALENLLS
LRHVAISPCVRKPGDLDMASLTLAEEFAKLESIAGLNAELAEAEEDLDGLADEALTWRLRQAAEARNRAVRSENEDKATY
DVGENGARLNRDERDAFGDLLKRIGFNSK

Sequences:

>Translated_669_residues
MSLPPGFLDELRTRISLSDVVGRKVMWDQRKSQPGKGDMWSPCPFHHEKTASFHVDDRKGFYYCFGCHAKGDALKFVQET
ENVSFMEAVEILAGEAGLEMPKRDPKAAEKQDRRTQLAEVMEQAVKYFRLQLKTQAASEARAYLARRGLDAAALDRWEIG
FAPDGWQNLWDALKGKDIADDLILGAGLAKPSNKGKKPYDVFRNRIMFPIRNARGRAIAFGGRAMDPNDNAKYLNSPETE
LFDKGRNLYNHGPARAASGRGSTLIVAEGYMDVIALSEAGFQSAVAPLGTAVTDNQLHLLWRMSDEPVIALDGDTAGLRA
AMRLIDMALPLLEAGKSLRFAIMPEGKDPDDLIRAEGPEAVQAVLDQAIPMVQLLWRRETEGRVFDSPERKAALDKSLRE
KIKLIRDPSIRLHYANDIKELRFQLFRGQRGGGGQGGTGYGQRYGGFKPGSRMQGGFRDGARMPWGAPPPPRSTTRASMV
ASSEEQDFARVREAVILATAITTPTVIPEFEINLERMQCQDMEHAHLRDLVLRYGIEAPERLRDEIAYALGPDALENLLS
LRHVAISPCVRKPGDLDMASLTLAEEFAKLESIAGLNAELAEAEEDLDGLADEALTWRLRQAAEARNRAVRSENEDKATY
DVGENGARLNRDERDAFGDLLKRIGFNSK
>Mature_668_residues
SLPPGFLDELRTRISLSDVVGRKVMWDQRKSQPGKGDMWSPCPFHHEKTASFHVDDRKGFYYCFGCHAKGDALKFVQETE
NVSFMEAVEILAGEAGLEMPKRDPKAAEKQDRRTQLAEVMEQAVKYFRLQLKTQAASEARAYLARRGLDAAALDRWEIGF
APDGWQNLWDALKGKDIADDLILGAGLAKPSNKGKKPYDVFRNRIMFPIRNARGRAIAFGGRAMDPNDNAKYLNSPETEL
FDKGRNLYNHGPARAASGRGSTLIVAEGYMDVIALSEAGFQSAVAPLGTAVTDNQLHLLWRMSDEPVIALDGDTAGLRAA
MRLIDMALPLLEAGKSLRFAIMPEGKDPDDLIRAEGPEAVQAVLDQAIPMVQLLWRRETEGRVFDSPERKAALDKSLREK
IKLIRDPSIRLHYANDIKELRFQLFRGQRGGGGQGGTGYGQRYGGFKPGSRMQGGFRDGARMPWGAPPPPRSTTRASMVA
SSEEQDFARVREAVILATAITTPTVIPEFEINLERMQCQDMEHAHLRDLVLRYGIEAPERLRDEIAYALGPDALENLLSL
RHVAISPCVRKPGDLDMASLTLAEEFAKLESIAGLNAELAEAEEDLDGLADEALTWRLRQAAEARNRAVRSENEDKATYD
VGENGARLNRDERDAFGDLLKRIGFNSK

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=419, Percent_Identity=37.708830548926, Blast_Score=295, Evalue=8e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 74278; Mature: 74147

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPPGFLDELRTRISLSDVVGRKVMWDQRKSQPGKGDMWSPCPFHHEKTASFHVDDRKG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCC
FYYCFGCHAKGDALKFVQETENVSFMEAVEILAGEAGLEMPKRDPKAAEKQDRRTQLAEV
EEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
MEQAVKYFRLQLKTQAASEARAYLARRGLDAAALDRWEIGFAPDGWQNLWDALKGKDIAD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHCCCCCHH
DLILGAGLAKPSNKGKKPYDVFRNRIMFPIRNARGRAIAFGGRAMDPNDNAKYLNSPETE
HHHHHCCCCCCCCCCCCHHHHHHCCEECEEECCCCCEEEECCCCCCCCCCCCCCCCCHHH
LFDKGRNLYNHGPARAASGRGSTLIVAEGYMDVIALSEAGFQSAVAPLGTAVTDNQLHLL
HHHCCCCHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHCCHHHHHHHHCCCCCCCCEEEE
WRMSDEPVIALDGDTAGLRAAMRLIDMALPLLEAGKSLRFAIMPEGKDPDDLIRAEGPEA
EEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCHHH
VQAVLDQAIPMVQLLWRRETEGRVFDSPERKAALDKSLREKIKLIRDPSIRLHYANDIKE
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHH
LRFQLFRGQRGGGGQGGTGYGQRYGGFKPGSRMQGGFRDGARMPWGAPPPPRSTTRASMV
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
ASSEEQDFARVREAVILATAITTPTVIPEFEINLERMQCQDMEHAHLRDLVLRYGIEAPE
HCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCCHH
RLRDEIAYALGPDALENLLSLRHVAISPCVRKPGDLDMASLTLAEEFAKLESIAGLNAEL
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHH
AEAEEDLDGLADEALTWRLRQAAEARNRAVRSENEDKATYDVGENGARLNRDERDAFGDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCHHHHHHHHH
LKRIGFNSK
HHHCCCCCC
>Mature Secondary Structure 
SLPPGFLDELRTRISLSDVVGRKVMWDQRKSQPGKGDMWSPCPFHHEKTASFHVDDRKG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCC
FYYCFGCHAKGDALKFVQETENVSFMEAVEILAGEAGLEMPKRDPKAAEKQDRRTQLAEV
EEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
MEQAVKYFRLQLKTQAASEARAYLARRGLDAAALDRWEIGFAPDGWQNLWDALKGKDIAD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHCCCCCHH
DLILGAGLAKPSNKGKKPYDVFRNRIMFPIRNARGRAIAFGGRAMDPNDNAKYLNSPETE
HHHHHCCCCCCCCCCCCHHHHHHCCEECEEECCCCCEEEECCCCCCCCCCCCCCCCCHHH
LFDKGRNLYNHGPARAASGRGSTLIVAEGYMDVIALSEAGFQSAVAPLGTAVTDNQLHLL
HHHCCCCHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHCCHHHHHHHHCCCCCCCCEEEE
WRMSDEPVIALDGDTAGLRAAMRLIDMALPLLEAGKSLRFAIMPEGKDPDDLIRAEGPEA
EEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCHHH
VQAVLDQAIPMVQLLWRRETEGRVFDSPERKAALDKSLREKIKLIRDPSIRLHYANDIKE
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHH
LRFQLFRGQRGGGGQGGTGYGQRYGGFKPGSRMQGGFRDGARMPWGAPPPPRSTTRASMV
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
ASSEEQDFARVREAVILATAITTPTVIPEFEINLERMQCQDMEHAHLRDLVLRYGIEAPE
HCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCCHH
RLRDEIAYALGPDALENLLSLRHVAISPCVRKPGDLDMASLTLAEEFAKLESIAGLNAEL
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHH
AEAEEDLDGLADEALTWRLRQAAEARNRAVRSENEDKATYDVGENGARLNRDERDAFGDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCHHHHHHHHH
LKRIGFNSK
HHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]