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The map label for this gene is queF

Identifier: 99081884

GI number: 99081884

Start: 2150402

End: 2150866

Strand: Reverse

Name: queF

Synonym: TM1040_2044

Alternate gene names: 99081884

Gene position: 2150866-2150402 (Counterclockwise)

Preceding gene: 99081885

Following gene: 99081883

Centisome position: 67.19

GC content: 61.08

Gene sequence:

>465_bases
ATGTCTGAAGATATTTACCAAAACCTCAAACAACTGGGCGGTGAGACCCGCATCCCTGCCAGCCCCGAAGAGGCCGAGCT
GGAACGGGTGGCAAACCCGCAGGCAGATGTGGCCTATAACGTGCGCTTCACCGCGCCGGAGTTCACCTCGCTCTGTCCGA
TGACGGGCCAGCCGGACTTTGCCCATCTGGTGATCGACTATGTTCCTGGCCCGTGGCTGGTGGAATCAAAGTCGCTCAAG
CTCTTCCTGACCTCCTTTCGCAACCACGGCGCCTTTCACGAGGATTGCACCATCTCGATCGCGCGGCGGCTGGTCGACTT
TCTCGACCCGCAATGGCTGCGGATCGGGGGCTATTGGTATCCGCGCGGCGGTATCCCGATTGATGTCTTTTGGCAGTCCG
GCACGATCCCCGAGGGTGTCTGGATTCCCGATCAGGGTGTGCCGCCCTATCGCGGTCGGGGCTGA

Upstream 100 bases:

>100_bases
CGGGCCATGGGCGAGCGGTGGTTTGCGCCTCGTATTCTGCCGCAACTGCATGTCCTCATCTGGGGCAACAAACGTGGCGT
CTGATGTGATGGAGAGTTCA

Downstream 100 bases:

>100_bases
GGGCCGCCCTCGGTTTTGATAGAACTGCACCCGTTGCGCCGGGCCGTGTCCCGGCGCGTCGGGGCGCTCTTGCGGACCGC
TAGAAACGGCTTTCCTCCTC

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MSEDIYQNLKQLGGETRIPASPEEAELERVANPQADVAYNVRFTAPEFTSLCPMTGQPDFAHLVIDYVPGPWLVESKSLK
LFLTSFRNHGAFHEDCTISIARRLVDFLDPQWLRIGGYWYPRGGIPIDVFWQSGTIPEGVWIPDQGVPPYRGRG

Sequences:

>Translated_154_residues
MSEDIYQNLKQLGGETRIPASPEEAELERVANPQADVAYNVRFTAPEFTSLCPMTGQPDFAHLVIDYVPGPWLVESKSLK
LFLTSFRNHGAFHEDCTISIARRLVDFLDPQWLRIGGYWYPRGGIPIDVFWQSGTIPEGVWIPDQGVPPYRGRG
>Mature_153_residues
SEDIYQNLKQLGGETRIPASPEEAELERVANPQADVAYNVRFTAPEFTSLCPMTGQPDFAHLVIDYVPGPWLVESKSLKL
FLTSFRNHGAFHEDCTISIARRLVDFLDPQWLRIGGYWYPRGGIPIDVFWQSGTIPEGVWIPDQGVPPYRGRG

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_SILST (Q1GEZ0)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_614038.1
- ProteinModelPortal:   Q1GEZ0
- SMR:   Q1GEZ0
- STRING:   Q1GEZ0
- GeneID:   4077971
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_2044
- NMPDR:   fig|292414.1.peg.416
- eggNOG:   COG0780
- HOGENOM:   HBG294103
- OMA:   HEDCTVA
- ProtClustDB:   PRK13258
- BioCyc:   SSP292414:TM1040_2044-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00818
- InterPro:   IPR016856
- InterPro:   IPR020602
- PIRSF:   PIRSF027377
- TIGRFAMs:   TIGR03139

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 17393; Mature: 17262

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEDIYQNLKQLGGETRIPASPEEAELERVANPQADVAYNVRFTAPEFTSLCPMTGQPDF
CCHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCHHCCCCCCCCCC
AHLVIDYVPGPWLVESKSLKLFLTSFRNHGAFHEDCTISIARRLVDFLDPQWLRIGGYWY
EEEEEECCCCCEEEECCCEEEEEEHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEECCEEE
PRGGIPIDVFWQSGTIPEGVWIPDQGVPPYRGRG
CCCCCEEEEEECCCCCCCCEECCCCCCCCCCCCC
>Mature Secondary Structure 
SEDIYQNLKQLGGETRIPASPEEAELERVANPQADVAYNVRFTAPEFTSLCPMTGQPDF
CHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCHHCCCCCCCCCC
AHLVIDYVPGPWLVESKSLKLFLTSFRNHGAFHEDCTISIARRLVDFLDPQWLRIGGYWY
EEEEEECCCCCEEEECCCEEEEEEHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEECCEEE
PRGGIPIDVFWQSGTIPEGVWIPDQGVPPYRGRG
CCCCCEEEEEECCCCCCCCEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA