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The map label for this gene is queE [H]

Identifier: 99081885

GI number: 99081885

Start: 2150883

End: 2151590

Strand: Reverse

Name: queE [H]

Synonym: TM1040_2045

Alternate gene names: 99081885

Gene position: 2151590-2150883 (Counterclockwise)

Preceding gene: 99081886

Following gene: 99081884

Centisome position: 67.22

GC content: 65.11

Gene sequence:

>708_bases
ATGACCCTCAGGATCGCAGAGATTTTTGGCCCGACGATTCAGGGCGAGGGGGCCTTGATTGGCGAACCCACGGTCTTTGT
GCGCGCGGGCGGCTGCGATTATCGCTGCAGCTGGTGCGACAGCCTGCATGCGGTGGACAGCGCGCACCGCCACAGCTGGG
CCGCGATGGAGACCGAAGCCGTCTGGCAGGAGGTGCGCCGCCTCTCGGGAGACCAGCCCCTGACCGTGTCGCTCTCGGGC
GGCAACCCGGCAATCCAGGACTTCGGCCCGCTCATCGCGCGGGGAAAGGCGGCGGGCTATCGGTTTGCCTGCGAAACCCA
AGGCTCGATCCCGCGACCGTGGTTCTCCGATCTTGAGACGCTGGTGCTTTCGCCCAAACCGCCCTCCAGTGGGGAAGACG
TGGATTGGGGCGCCTTTATTGCCTGTCGCAAAATCGGCGCAGGGGCGCGCCAGCAGGTGATGAAGATCGTGATCTTTGAT
GAGGCCGACTATCTCTGGGCGCGGGAGGTTCACCGCCGCCACAGTGACCTGCCGCTGTATCTGCAGCCCGGCAACCCCGA
GGTCGACCCCGAAACCCCTGTGGATCTCAATGCACAGGCGGAGCGCCTGCGCTGGCTCACGGAACGGGCCATGGGCGAGC
GGTGGTTTGCGCCTCGTATTCTGCCGCAACTGCATGTCCTCATCTGGGGCAACAAACGTGGCGTCTGA

Upstream 100 bases:

>100_bases
ACATGGCACGCCATTTCTACGAGTGGTGCGCCGCGCGCTGGCCCGAAACCAGCGCGGTCCTCGTGAGCGAGACCCCAAAG
ACCTGGGCGGAGTACCGGCC

Downstream 100 bases:

>100_bases
TGTGATGGAGAGTTCAATGTCTGAAGATATTTACCAAAACCTCAAACAACTGGGCGGTGAGACCCGCATCCCTGCCAGCC
CCGAAGAGGCCGAGCTGGAA

Product: radical SAM family protein

Products: NA

Alternate protein names: CDG synthase; Queuosine biosynthesis protein queE [H]

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MTLRIAEIFGPTIQGEGALIGEPTVFVRAGGCDYRCSWCDSLHAVDSAHRHSWAAMETEAVWQEVRRLSGDQPLTVSLSG
GNPAIQDFGPLIARGKAAGYRFACETQGSIPRPWFSDLETLVLSPKPPSSGEDVDWGAFIACRKIGAGARQQVMKIVIFD
EADYLWAREVHRRHSDLPLYLQPGNPEVDPETPVDLNAQAERLRWLTERAMGERWFAPRILPQLHVLIWGNKRGV

Sequences:

>Translated_235_residues
MTLRIAEIFGPTIQGEGALIGEPTVFVRAGGCDYRCSWCDSLHAVDSAHRHSWAAMETEAVWQEVRRLSGDQPLTVSLSG
GNPAIQDFGPLIARGKAAGYRFACETQGSIPRPWFSDLETLVLSPKPPSSGEDVDWGAFIACRKIGAGARQQVMKIVIFD
EADYLWAREVHRRHSDLPLYLQPGNPEVDPETPVDLNAQAERLRWLTERAMGERWFAPRILPQLHVLIWGNKRGV
>Mature_234_residues
TLRIAEIFGPTIQGEGALIGEPTVFVRAGGCDYRCSWCDSLHAVDSAHRHSWAAMETEAVWQEVRRLSGDQPLTVSLSGG
NPAIQDFGPLIARGKAAGYRFACETQGSIPRPWFSDLETLVLSPKPPSSGEDVDWGAFIACRKIGAGARQQVMKIVIFDE
ADYLWAREVHRRHSDLPLYLQPGNPEVDPETPVDLNAQAERLRWLTERAMGERWFAPRILPQLHVLIWGNKRGV

Specific function: Catalyzes the conversion of 6-carboxy-5,6,7,8- tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG) [H]

COG id: COG0602

COG function: function code O; Organic radical activating enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. 7-carboxy-7- deazaguanine synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017742
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 26199; Mature: 26068

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLRIAEIFGPTIQGEGALIGEPTVFVRAGGCDYRCSWCDSLHAVDSAHRHSWAAMETEA
CEEEEEEHHCCCCCCCCEEECCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
VWQEVRRLSGDQPLTVSLSGGNPAIQDFGPLIARGKAAGYRFACETQGSIPRPWFSDLET
HHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHH
LVLSPKPPSSGEDVDWGAFIACRKIGAGARQQVMKIVIFDEADYLWAREVHRRHSDLPLY
EEECCCCCCCCCCCCHHHHHHHHHHCCCHHHHEEEEEEECCCCHHHHHHHHHHCCCCCEE
LQPGNPEVDPETPVDLNAQAERLRWLTERAMGERWFAPRILPQLHVLIWGNKRGV
EECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHCCEEEEEEECCCCCC
>Mature Secondary Structure 
TLRIAEIFGPTIQGEGALIGEPTVFVRAGGCDYRCSWCDSLHAVDSAHRHSWAAMETEA
EEEEEEHHCCCCCCCCEEECCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
VWQEVRRLSGDQPLTVSLSGGNPAIQDFGPLIARGKAAGYRFACETQGSIPRPWFSDLET
HHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHH
LVLSPKPPSSGEDVDWGAFIACRKIGAGARQQVMKIVIFDEADYLWAREVHRRHSDLPLY
EEECCCCCCCCCCCCHHHHHHHHHHCCCHHHHEEEEEEECCCCHHHHHHHHHHCCCCCEE
LQPGNPEVDPETPVDLNAQAERLRWLTERAMGERWFAPRILPQLHVLIWGNKRGV
EECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]