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The map label for this gene is pcm [H]

Identifier: 99081832

GI number: 99081832

Start: 2097720

End: 2098373

Strand: Reverse

Name: pcm [H]

Synonym: TM1040_1992

Alternate gene names: 99081832

Gene position: 2098373-2097720 (Counterclockwise)

Preceding gene: 99081833

Following gene: 99081831

Centisome position: 65.55

GC content: 61.93

Gene sequence:

>654_bases
ATGACTGATTTCTCCGACCGCCGCCGCATGATGGTAGACACCCAGATCCGTCCGGCGGATGTGACCAAATACCCGATCAT
CGAAGCCTTGCTGGCCATTTCGCGCGAAAAATTCGTCCCGGATTCCCAGGCCGAGGTGGCCTATGCCGATCAATCCGTAC
CGCTGAGCACGGGCCGCGTGGTGCCTGAGCCGCGCACGCTTGCCAAGATGCTGGACGCGCTTGATGTGCGGCAGGATGAA
CTGGTGCTCGATGTGGCCTGCGGCTTTGGCTATTCCACCGCCGTGGTTGCGCGTCTCGCGCAGATGGTGATCGGGGTGGA
AGAAGACGAAAGCCTGGCCTCCGAGGCGCAAGAGATCCTGAGCGCCAGCAACGCCGACAATGCGATCGTGCATCAGGGTG
ATCTGGCCGAGGGCGCTGCCGAACATGGCCCTTATGATGTGATCATGATCGAGGGCGGCGTCGAAGAGGTGCCTGAGGCG
CTTCTGGCGCAGCTCAAGGATGGTGGTCGCATCGCGGCCCTGTTCATGGACGGCGCTCTGGGCGAGGTGAAAATCGGCTA
CAAGAGCAATGGGCGCGTGTCCTGGCGTTTTGAATTCAATGCCGGCGCGCCGGTGCTGCCGGGCTTTGCCAAGCCGAGGG
TCTTTTCGCTCTGA

Upstream 100 bases:

>100_bases
CAGGTTTGCCAAACCTTTGCTATTGTCAAAAGGGGCCGTTGCCGCGCGGGTGGCGATGTGGCAAAACCCACTCGAATGCC
TGAACATATGAGTTTAGCAG

Downstream 100 bases:

>100_bases
GGGCGGCGATCCTGTCGCGCTCGAGTCTTTTTGGCCACATCTGCAAGGCAGGCTCTTTTCAAATTGGTGTGACGTTCACA
TATGTGTGACGCAAACGAGA

Product: protein-L-isoaspartate(D-aspartate) O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MTDFSDRRRMMVDTQIRPADVTKYPIIEALLAISREKFVPDSQAEVAYADQSVPLSTGRVVPEPRTLAKMLDALDVRQDE
LVLDVACGFGYSTAVVARLAQMVIGVEEDESLASEAQEILSASNADNAIVHQGDLAEGAAEHGPYDVIMIEGGVEEVPEA
LLAQLKDGGRIAALFMDGALGEVKIGYKSNGRVSWRFEFNAGAPVLPGFAKPRVFSL

Sequences:

>Translated_217_residues
MTDFSDRRRMMVDTQIRPADVTKYPIIEALLAISREKFVPDSQAEVAYADQSVPLSTGRVVPEPRTLAKMLDALDVRQDE
LVLDVACGFGYSTAVVARLAQMVIGVEEDESLASEAQEILSASNADNAIVHQGDLAEGAAEHGPYDVIMIEGGVEEVPEA
LLAQLKDGGRIAALFMDGALGEVKIGYKSNGRVSWRFEFNAGAPVLPGFAKPRVFSL
>Mature_216_residues
TDFSDRRRMMVDTQIRPADVTKYPIIEALLAISREKFVPDSQAEVAYADQSVPLSTGRVVPEPRTLAKMLDALDVRQDEL
VLDVACGFGYSTAVVARLAQMVIGVEEDESLASEAQEILSASNADNAIVHQGDLAEGAAEHGPYDVIMIEGGVEEVPEAL
LAQLKDGGRIAALFMDGALGEVKIGYKSNGRVSWRFEFNAGAPVLPGFAKPRVFSL

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789100, Length=165, Percent_Identity=30.9090909090909, Blast_Score=95, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 23411; Mature: 23280

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDFSDRRRMMVDTQIRPADVTKYPIIEALLAISREKFVPDSQAEVAYADQSVPLSTGRV
CCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCC
VPEPRTLAKMLDALDVRQDELVLDVACGFGYSTAVVARLAQMVIGVEEDESLASEAQEIL
CCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
SASNADNAIVHQGDLAEGAAEHGPYDVIMIEGGVEEVPEALLAQLKDGGRIAALFMDGAL
HCCCCCCEEEECCCCCCCHHCCCCEEEEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCC
GEVKIGYKSNGRVSWRFEFNAGAPVLPGFAKPRVFSL
CEEEEEECCCCEEEEEEEECCCCCCCCCCCCCEEECC
>Mature Secondary Structure 
TDFSDRRRMMVDTQIRPADVTKYPIIEALLAISREKFVPDSQAEVAYADQSVPLSTGRV
CCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCC
VPEPRTLAKMLDALDVRQDELVLDVACGFGYSTAVVARLAQMVIGVEEDESLASEAQEIL
CCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
SASNADNAIVHQGDLAEGAAEHGPYDVIMIEGGVEEVPEALLAQLKDGGRIAALFMDGAL
HCCCCCCEEEECCCCCCCHHCCCCEEEEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCC
GEVKIGYKSNGRVSWRFEFNAGAPVLPGFAKPRVFSL
CEEEEEECCCCEEEEEEEECCCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA