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The map label for this gene is nthB [H]

Identifier: 99081811

GI number: 99081811

Start: 2075512

End: 2076216

Strand: Reverse

Name: nthB [H]

Synonym: TM1040_1971

Alternate gene names: 99081811

Gene position: 2076216-2075512 (Counterclockwise)

Preceding gene: 99081812

Following gene: 99081810

Centisome position: 64.86

GC content: 64.26

Gene sequence:

>705_bases
ATGAGCCGAATTCACGATATGGGGGGGCGCTATGGCGATGGTCCGATCACGCCCGAGCCCCTGGACGCGCCGGTCTTTCC
CGAGGACTGGCACGGCCGCGCGCTTGCGGTGACCTTGGCCTGTGGGGCGCTTGGGCAGTGGAATATCGACATCTCGCGCC
ATGCGCGCGAATGCCTCAGCCCCTCGGACTATATGAATTTCAGCTACTACGAGAAATGGCTGGCCGCCTTGGCGGATCTC
TTGGTGGCGCAGGGTGTATTGCGCCGCGAAGACCTCAGCGATGTAGATGAGGCCGCAAATGACTCTGACGCGGCGGAGCC
TGCCCATCCGCTGGCGGAGCGCTGTTTGTCCGCCGATCGCGTGGCCGCGGTTCTTGCGGCGGGTGGGCCTGCGGATCGCA
AAAGTCCGGTCGCCGCGCAGTATCAGCCCGGGGATTTTGTGCGCACGCGGCTGAGTGGCAACCGATTGGTCGCGGGCGGT
CATACCCGTTTGCCCACCTACGCAAGCCACGCTCAGGGGCAGATCCTGCGCCTGCATGGCACGCATGTTTTCCCTGATTC
CAATGCCCACGGTCAGGGGGAGGCGCCCGAGCCCCTCTATGCGGTGGCCTTTGCCGCGCGCGACCTCTGGCCGCAGGCAG
AGCACCCCAATGATGAGGTGGTGTTGGATCTCTGGCAAAGCTATCTGGAGCCGGTTTCATCATGA

Upstream 100 bases:

>100_bases
AAGGCACCGACGGCTGGAGCGAAGAGGCGTTGATGACCCTGGTCAACCGCGATTCGATGATCGGAACCGCCCTTGCCACC
CCAGCGAGCGAGGCCCCTGC

Downstream 100 bases:

>100_bases
CGGTGCCCGAGACCAAGCTGAATACAGCTGATCAGATGCAGCGCCCCGAGCCCGCGTTCGAGGCCCCCTGGCACGCACAG
GCCTTTGCGCTGGCGGTCCA

Product: nitrile hydratase

Products: NA

Alternate protein names: NHase; Nitrilase [H]

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MSRIHDMGGRYGDGPITPEPLDAPVFPEDWHGRALAVTLACGALGQWNIDISRHARECLSPSDYMNFSYYEKWLAALADL
LVAQGVLRREDLSDVDEAANDSDAAEPAHPLAERCLSADRVAAVLAAGGPADRKSPVAAQYQPGDFVRTRLSGNRLVAGG
HTRLPTYASHAQGQILRLHGTHVFPDSNAHGQGEAPEPLYAVAFAARDLWPQAEHPNDEVVLDLWQSYLEPVSS

Sequences:

>Translated_234_residues
MSRIHDMGGRYGDGPITPEPLDAPVFPEDWHGRALAVTLACGALGQWNIDISRHARECLSPSDYMNFSYYEKWLAALADL
LVAQGVLRREDLSDVDEAANDSDAAEPAHPLAERCLSADRVAAVLAAGGPADRKSPVAAQYQPGDFVRTRLSGNRLVAGG
HTRLPTYASHAQGQILRLHGTHVFPDSNAHGQGEAPEPLYAVAFAARDLWPQAEHPNDEVVLDLWQSYLEPVSS
>Mature_233_residues
SRIHDMGGRYGDGPITPEPLDAPVFPEDWHGRALAVTLACGALGQWNIDISRHARECLSPSDYMNFSYYEKWLAALADLL
VAQGVLRREDLSDVDEAANDSDAAEPAHPLAERCLSADRVAAVLAAGGPADRKSPVAAQYQPGDFVRTRLSGNRLVAGGH
TRLPTYASHAQGQILRLHGTHVFPDSNAHGQGEAPEPLYAVAFAARDLWPQAEHPNDEVVLDLWQSYLEPVSS

Specific function: NHase catalyzes the hydration of various nitrile compounds to the corresponding amides [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrile hydratase subunit beta family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008990
- InterPro:   IPR003168 [H]

Pfam domain/function: NA

EC number: =4.2.1.84 [H]

Molecular weight: Translated: 25396; Mature: 25265

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIHDMGGRYGDGPITPEPLDAPVFPEDWHGRALAVTLACGALGQWNIDISRHARECLS
CCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHCCCCCCCCCHHHHHHHHCC
PSDYMNFSYYEKWLAALADLLVAQGVLRREDLSDVDEAANDSDAAEPAHPLAERCLSADR
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
VAAVLAAGGPADRKSPVAAQYQPGDFVRTRLSGNRLVAGGHTRLPTYASHAQGQILRLHG
HHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEECCCCCCCCHHHCCCCEEEEEEC
THVFPDSNAHGQGEAPEPLYAVAFAARDLWPQAEHPNDEVVLDLWQSYLEPVSS
CEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SRIHDMGGRYGDGPITPEPLDAPVFPEDWHGRALAVTLACGALGQWNIDISRHARECLS
CCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHCCCCCCCCCHHHHHHHHCC
PSDYMNFSYYEKWLAALADLLVAQGVLRREDLSDVDEAANDSDAAEPAHPLAERCLSADR
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
VAAVLAAGGPADRKSPVAAQYQPGDFVRTRLSGNRLVAGGHTRLPTYASHAQGQILRLHG
HHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEECCCCCCCCHHHCCCCEEEEEEC
THVFPDSNAHGQGEAPEPLYAVAFAARDLWPQAEHPNDEVVLDLWQSYLEPVSS
CEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9154927 [H]