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The map label for this gene is nthA [H]

Identifier: 99081812

GI number: 99081812

Start: 2076213

End: 2076839

Strand: Reverse

Name: nthA [H]

Synonym: TM1040_1972

Alternate gene names: 99081812

Gene position: 2076839-2076213 (Counterclockwise)

Preceding gene: 99081817

Following gene: 99081811

Centisome position: 64.88

GC content: 63.0

Gene sequence:

>627_bases
ATGCCTCATGATCATCACGACCACCCGCACAGTCTTCTTCCTTCTGAACCTGCCCTGCGCGTCAAAGCGCTGGAGACGAT
TCTGACCCGCAAAGGGCTCATTGACCCGGCCGCGCTGGACGCGATCATCGACACCTACGAAAACAAGGTTGGACCCCAAA
ATGGCGCGCGTGTTGTGGCGCGGGCCTGGTCGGACCCTGCGTTTCGCGCCGCATTGCTCGAGGATGCGGACAAGATCCTC
GATGAGATGGCGCTGAACGGGCGGCAGGGGGAACATGTTGTGGTGGTGGAGAACACACCCGAGCGGCACAACATGGTCGT
ATGCACCCTGTGCAGCTGCTATCCATGGCCACTCTTGGGCATTCCGCCGGGCTGGTACAAATCCGATGCCTATCGCGCCC
GCGCCGTGCGCGAGCCCCGCAAGGTGCTGGCGGAATTTGGCGTCACCCTGCCGGCGGACACATCGGTCAGAGTGTGGGAT
TCCACAGCCGAGGTGCGCTATCTGGTGCTGCCCATGCGTCCCGAAGGCACCGACGGCTGGAGCGAAGAGGCGTTGATGAC
CCTGGTCAACCGCGATTCGATGATCGGAACCGCCCTTGCCACCCCAGCGAGCGAGGCCCCTGCATGA

Upstream 100 bases:

>100_bases
TTGCCCTGAGGAGCGGAAAACCTCGCGCCAACAGGATTTGATTGTATGCATCACGATTTGCAACTGGACAGGGGCACGCC
CCTGCGGCCAGCATCTGCGC

Downstream 100 bases:

>100_bases
GCCGAATTCACGATATGGGGGGGCGCTATGGCGATGGTCCGATCACGCCCGAGCCCCTGGACGCGCCGGTCTTTCCCGAG
GACTGGCACGGCCGCGCGCT

Product: nitrile hydratase, alpha subunit

Products: NA

Alternate protein names: NHase; Nitrilase [H]

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MPHDHHDHPHSLLPSEPALRVKALETILTRKGLIDPAALDAIIDTYENKVGPQNGARVVARAWSDPAFRAALLEDADKIL
DEMALNGRQGEHVVVVENTPERHNMVVCTLCSCYPWPLLGIPPGWYKSDAYRARAVREPRKVLAEFGVTLPADTSVRVWD
STAEVRYLVLPMRPEGTDGWSEEALMTLVNRDSMIGTALATPASEAPA

Sequences:

>Translated_208_residues
MPHDHHDHPHSLLPSEPALRVKALETILTRKGLIDPAALDAIIDTYENKVGPQNGARVVARAWSDPAFRAALLEDADKIL
DEMALNGRQGEHVVVVENTPERHNMVVCTLCSCYPWPLLGIPPGWYKSDAYRARAVREPRKVLAEFGVTLPADTSVRVWD
STAEVRYLVLPMRPEGTDGWSEEALMTLVNRDSMIGTALATPASEAPA
>Mature_207_residues
PHDHHDHPHSLLPSEPALRVKALETILTRKGLIDPAALDAIIDTYENKVGPQNGARVVARAWSDPAFRAALLEDADKILD
EMALNGRQGEHVVVVENTPERHNMVVCTLCSCYPWPLLGIPPGWYKSDAYRARAVREPRKVLAEFGVTLPADTSVRVWDS
TAEVRYLVLPMRPEGTDGWSEEALMTLVNRDSMIGTALATPASEAPA

Specific function: NHase catalyzes the hydration of various nitrile compounds to the corresponding amides. Industrial production of acrylamide is now being developed using some of the enzymes of this class [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrile hydratase subunit alpha family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004232
- InterPro:   IPR018141
- ProDom:   PD007559 [H]

Pfam domain/function: PF02979 NHase_alpha [H]

EC number: =4.2.1.84 [H]

Molecular weight: Translated: 22906; Mature: 22775

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHDHHDHPHSLLPSEPALRVKALETILTRKGLIDPAALDAIIDTYENKVGPQNGARVVA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
RAWSDPAFRAALLEDADKILDEMALNGRQGEHVVVVENTPERHNMVVCTLCSCYPWPLLG
ECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHCEEEEEECCCCCCCCCC
IPPGWYKSDAYRARAVREPRKVLAEFGVTLPADTSVRVWDSTAEVRYLVLPMRPEGTDGW
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCC
SEEALMTLVNRDSMIGTALATPASEAPA
HHHHHHHHHHCCCHHHHHHCCCCCCCCC
>Mature Secondary Structure 
PHDHHDHPHSLLPSEPALRVKALETILTRKGLIDPAALDAIIDTYENKVGPQNGARVVA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
RAWSDPAFRAALLEDADKILDEMALNGRQGEHVVVVENTPERHNMVVCTLCSCYPWPLLG
ECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHCEEEEEECCCCCCCCCC
IPPGWYKSDAYRARAVREPRKVLAEFGVTLPADTSVRVWDSTAEVRYLVLPMRPEGTDGW
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCC
SEEALMTLVNRDSMIGTALATPASEAPA
HHHHHHHHHHCCCHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9154927 [H]