Click here to switch to the map view.

The map label for this gene is gsiB [H]

Identifier: 99081661

GI number: 99081661

Start: 1914075

End: 1915658

Strand: Direct

Name: gsiB [H]

Synonym: TM1040_1820

Alternate gene names: 99081661

Gene position: 1914075-1915658 (Clockwise)

Preceding gene: 99081660

Following gene: 99081662

Centisome position: 59.8

GC content: 62.5

Gene sequence:

>1584_bases
ATGAACGACTATCTTAAACACCTCGCACAGGAAGCCGCATTGGGCCGCATGGGCCGCCGCGAGTTCCTCGGCCGCGCTGC
TGCGCTGGGTGTGACCGCATTGGGCGCGAACTCGCTGCTGGCCTCTGCGGCCAAAGCGGCAGGCGCGCAAAAAGGCGGCA
CCCTGCGTGTGGGTCTGCAAGGGGGCTCCACCACCGACAGCCTCGACCCGGCTCTGGCGACAAACCAGGTGGCCCTCTCG
GTGATCCGTCTCTGGGGTGAGCCGCTGGTGGAACTGGGCGAAGAAGGCGGCCTCGTGGGTGCCGTCGCCGAAAGCTTTGA
AGCCTCGGCAGACGCAAAAACCTGGACCTTCAAGCTGCGCTCCGGCCTGACCTTCTCCAACGGCCAGCCGGTGACGGCGG
CGGATGTTGTCGCCACCATGGAGCGTCACTCCGGTGAGGACACAAAATCCGGTGCGCTTGGCATCATGCGCTCCATTTCC
AACGTCAAGGCCGATGGCGATACGGTGGTCTTTGAGCTCGAAGACGCAAACGCCGACCTGCCCTACCTGCTGACCGACTA
CCACCTGGTGATCCAGCCCAACGGCGGCAAGGACGATCCGGCGGCCGCGATCGGCACCGGTCCTTATGTTCTGAAAGCCG
TGGACATGGGCGTGCGCTTTGTGGCCGAGAAGAACCCCAACTACTGGGGCGATCTGGGCAATGCGCAGACCATCGAGTAC
GTGGTCATCAACGACAATACCGCCCGTGTGGCTGCACTGCAGGCCGGTCAGGTGGACATGATCGACCGCGTGCCGCCGCG
CACCGCGAAACTGGTGGATCGCGCGCCGAACATCTCCGTGCACTCCACTGCGGGTCCGGGCCACTATGTGTTCATCGCCC
ATTGCGACACCGATCCCTTTGCCAACAACGACGTGCGCCTCGCACTGAAATACGGCATCAACCGTCAGGAAATGGTCGAC
AAGATCCTGAACGGCTTTGGCTCCGTGGGCAACGACTCGCCGATCAACGCCTCCTACCCGCTGTTCACCCAGCTGGAGCA
GCGCGAGTACGATCCCGAGAAGGCCAAGTTCCACATGGAAAAATCGGGCTATGACGGTCCGATCCTGCTGCGCACCTCCG
ACAACTCCTTCCCCGGAGCCCCGGATGCGGCGGCGCTGTTCCAGCAGTCGCTGGCAGCGGCAGGCATCAACCTCGAGATC
AAGCGTGAGCCCAACGATGGCTACTGGTCCGAGGTCTGGAACAAGCAGCCCTTCTGCACCTCCTACTGGGGTGGCCGTCC
GACGCAGGACTCCATGTTCTCCACCGCCTATCTGTCGACCGCAGACTGGAACGACACCCGTTTCAAGAACGAGCAGTTCG
ACCAGACACTGCTGGCGGCCCGTGGCGAGCTCGACGAAGCCAAGCGCACCCAGATGTATGCAGATATGTCGACCATGGTG
CGCGACGAAGGCGGCCTGATCTGCCCGATGTTCAACGACTTTGTCGATGCAACGTCGGATCGTCTGGACGGCTGGAAAGA
CGGCGTCAAAGGTCACGCTCTCATGAACGGTTACGCGCCCCTGAAAATGTGGGTCAAAGCCTGA

Upstream 100 bases:

>100_bases
CTATCATTATTCCTTGCCCCGATGTGCCAAATCGGCAAATCTTGTATCACCATTCAAGAAAATCCGGGGCCAACCCGTAA
CCTCGACAGGGAGACAACGA

Downstream 100 bases:

>100_bases
TATGAATCCGGTGATCAAACTATTGGCTCAGCGCATTGCGCTGAGTCTCTTCCTGCTGTTGATGATCTCGGCGCTGATCT
TTGCGGGCACCATGATCCTG

Product: twin-arginine translocation pathway signal

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MNDYLKHLAQEAALGRMGRREFLGRAAALGVTALGANSLLASAAKAAGAQKGGTLRVGLQGGSTTDSLDPALATNQVALS
VIRLWGEPLVELGEEGGLVGAVAESFEASADAKTWTFKLRSGLTFSNGQPVTAADVVATMERHSGEDTKSGALGIMRSIS
NVKADGDTVVFELEDANADLPYLLTDYHLVIQPNGGKDDPAAAIGTGPYVLKAVDMGVRFVAEKNPNYWGDLGNAQTIEY
VVINDNTARVAALQAGQVDMIDRVPPRTAKLVDRAPNISVHSTAGPGHYVFIAHCDTDPFANNDVRLALKYGINRQEMVD
KILNGFGSVGNDSPINASYPLFTQLEQREYDPEKAKFHMEKSGYDGPILLRTSDNSFPGAPDAAALFQQSLAAAGINLEI
KREPNDGYWSEVWNKQPFCTSYWGGRPTQDSMFSTAYLSTADWNDTRFKNEQFDQTLLAARGELDEAKRTQMYADMSTMV
RDEGGLICPMFNDFVDATSDRLDGWKDGVKGHALMNGYAPLKMWVKA

Sequences:

>Translated_527_residues
MNDYLKHLAQEAALGRMGRREFLGRAAALGVTALGANSLLASAAKAAGAQKGGTLRVGLQGGSTTDSLDPALATNQVALS
VIRLWGEPLVELGEEGGLVGAVAESFEASADAKTWTFKLRSGLTFSNGQPVTAADVVATMERHSGEDTKSGALGIMRSIS
NVKADGDTVVFELEDANADLPYLLTDYHLVIQPNGGKDDPAAAIGTGPYVLKAVDMGVRFVAEKNPNYWGDLGNAQTIEY
VVINDNTARVAALQAGQVDMIDRVPPRTAKLVDRAPNISVHSTAGPGHYVFIAHCDTDPFANNDVRLALKYGINRQEMVD
KILNGFGSVGNDSPINASYPLFTQLEQREYDPEKAKFHMEKSGYDGPILLRTSDNSFPGAPDAAALFQQSLAAAGINLEI
KREPNDGYWSEVWNKQPFCTSYWGGRPTQDSMFSTAYLSTADWNDTRFKNEQFDQTLLAARGELDEAKRTQMYADMSTMV
RDEGGLICPMFNDFVDATSDRLDGWKDGVKGHALMNGYAPLKMWVKA
>Mature_527_residues
MNDYLKHLAQEAALGRMGRREFLGRAAALGVTALGANSLLASAAKAAGAQKGGTLRVGLQGGSTTDSLDPALATNQVALS
VIRLWGEPLVELGEEGGLVGAVAESFEASADAKTWTFKLRSGLTFSNGQPVTAADVVATMERHSGEDTKSGALGIMRSIS
NVKADGDTVVFELEDANADLPYLLTDYHLVIQPNGGKDDPAAAIGTGPYVLKAVDMGVRFVAEKNPNYWGDLGNAQTIEY
VVINDNTARVAALQAGQVDMIDRVPPRTAKLVDRAPNISVHSTAGPGHYVFIAHCDTDPFANNDVRLALKYGINRQEMVD
KILNGFGSVGNDSPINASYPLFTQLEQREYDPEKAKFHMEKSGYDGPILLRTSDNSFPGAPDAAALFQQSLAAAGINLEI
KREPNDGYWSEVWNKQPFCTSYWGGRPTQDSMFSTAYLSTADWNDTRFKNEQFDQTLLAARGELDEAKRTQMYADMSTMV
RDEGGLICPMFNDFVDATSDRLDGWKDGVKGHALMNGYAPLKMWVKA

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=504, Percent_Identity=26.5873015873016, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI87081878, Length=494, Percent_Identity=27.3279352226721, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1789887, Length=319, Percent_Identity=30.7210031347962, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1787495, Length=521, Percent_Identity=26.1036468330134, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787762, Length=465, Percent_Identity=23.6559139784946, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1789966, Length=431, Percent_Identity=22.969837587007, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1789397, Length=470, Percent_Identity=23.4042553191489, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 56951; Mature: 56951

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDYLKHLAQEAALGRMGRREFLGRAAALGVTALGANSLLASAAKAAGAQKGGTLRVGLQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEC
GGSTTDSLDPALATNQVALSVIRLWGEPLVELGEEGGLVGAVAESFEASADAKTWTFKLR
CCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCEEHHHHHHHHCCCCCEEEEEEEE
SGLTFSNGQPVTAADVVATMERHSGEDTKSGALGIMRSISNVKADGDTVVFELEDANADL
CCCEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCC
PYLLTDYHLVIQPNGGKDDPAAAIGTGPYVLKAVDMGVRFVAEKNPNYWGDLGNAQTIEY
CEEEECEEEEEECCCCCCCCCEEECCCCCEEEEHHCCEEEEEECCCCCCCCCCCCCEEEE
VVINDNTARVAALQAGQVDMIDRVPPRTAKLVDRAPNISVHSTAGPGHYVFIAHCDTDPF
EEECCCCEEEEEEECCCEEHHHCCCCHHHHHHHCCCCCEEECCCCCCEEEEEEECCCCCC
ANNDVRLALKYGINRQEMVDKILNGFGSVGNDSPINASYPLFTQLEQREYDPEKAKFHME
CCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHEEEC
KSGYDGPILLRTSDNSFPGAPDAAALFQQSLAAAGINLEIKREPNDGYWSEVWNKQPFCT
CCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHCCCCCCEE
SYWGGRPTQDSMFSTAYLSTADWNDTRFKNEQFDQTLLAARGELDEAKRTQMYADMSTMV
CCCCCCCCCCCHHEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RDEGGLICPMFNDFVDATSDRLDGWKDGVKGHALMNGYAPLKMWVKA
HCCCCEECCCHHHHHHHHHHHCCHHHCCCCCCEEECCCCCEEEEECC
>Mature Secondary Structure
MNDYLKHLAQEAALGRMGRREFLGRAAALGVTALGANSLLASAAKAAGAQKGGTLRVGLQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEC
GGSTTDSLDPALATNQVALSVIRLWGEPLVELGEEGGLVGAVAESFEASADAKTWTFKLR
CCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCEEHHHHHHHHCCCCCEEEEEEEE
SGLTFSNGQPVTAADVVATMERHSGEDTKSGALGIMRSISNVKADGDTVVFELEDANADL
CCCEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCC
PYLLTDYHLVIQPNGGKDDPAAAIGTGPYVLKAVDMGVRFVAEKNPNYWGDLGNAQTIEY
CEEEECEEEEEECCCCCCCCCEEECCCCCEEEEHHCCEEEEEECCCCCCCCCCCCCEEEE
VVINDNTARVAALQAGQVDMIDRVPPRTAKLVDRAPNISVHSTAGPGHYVFIAHCDTDPF
EEECCCCEEEEEEECCCEEHHHCCCCHHHHHHHCCCCCEEECCCCCCEEEEEEECCCCCC
ANNDVRLALKYGINRQEMVDKILNGFGSVGNDSPINASYPLFTQLEQREYDPEKAKFHME
CCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHEEEC
KSGYDGPILLRTSDNSFPGAPDAAALFQQSLAAAGINLEIKREPNDGYWSEVWNKQPFCT
CCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHCCCCCCEE
SYWGGRPTQDSMFSTAYLSTADWNDTRFKNEQFDQTLLAARGELDEAKRTQMYADMSTMV
CCCCCCCCCCCHHEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RDEGGLICPMFNDFVDATSDRLDGWKDGVKGHALMNGYAPLKMWVKA
HCCCCEECCCHHHHHHHHHHHCCHHHCCCCCCEEECCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA