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The map label for this gene is prfC [H]

Identifier: 99081648

GI number: 99081648

Start: 1897799

End: 1899487

Strand: Direct

Name: prfC [H]

Synonym: TM1040_1807

Alternate gene names: 99081648

Gene position: 1897799-1899487 (Clockwise)

Preceding gene: 99081645

Following gene: 99081649

Centisome position: 59.29

GC content: 60.45

Gene sequence:

>1689_bases
ATGCGCCCAGTGAAACACATCGCACCCGTTCAGCACGGCTTTTCCTTGCCTCTGAGCCGCTTTGTGGCTAAGGCCAGCCT
CATGTTGGACACCGCTCATAACCGCCCCGATCTGCCGCCGGAAATCGCGCGGCGCCGCACCTTTGCGATCATCTCGCACC
CGGATGCGGGCAAGACCACTCTGACCGAGAAGTTCCTCCTTTATGGGGGCGCCATCCAGATGGCGGGTCAGGTGCGCGCC
AAGGGCGAAGCGCGGCGCACGCGTTCTGACTTCATGCAGATGGAAAAGGATCGGGGCATCTCGGTTTCGGCCTCCGCAAT
GTCCTTTGACTACGGGGATTTCCGCTACAACCTCGTCGACACGCCCGGCCACTCGGACTTTTCCGAGGACACCTACCGCA
CGCTGACGGCGGTGGACGCGGCGGTGATGGTGATTGATGGCGCCAAGGGTGTGGAAAGCCAGACCCAGAAGCTCTTTGAA
GTTTGCCGCCTGCGCGATCTGCCGATCCTGACCTTCTGTAACAAGATGGACCGCGAGAGCCGCGATACCTTCGACATTAT
CGACGAGATTCAGGAAAACCTCGCCATCGACGTCACCCCGGCGAGCTGGCCTATCGGCGTCGGGCGTGACTTTATCGGCT
GTTATGACCTCTTGCGCGACCGGCTCGAACTGATGGATCGTGCTGACCGCAACAAGGTGGCAGAGAGCATCTCGATCAAC
GGGCTTGATGATCCCAAACTGGCCGAGCACGTCCCGGAGCATCTGTTGGAAAAGCTGCTCGAAGAGGTCGAAATGGCGCG
CGAGCTTTTGCCGACGCTGGACCCGCAAGCGGTGCTCGAGGGGCATATGACCCCGATCTGGTTCGGCTCTGCGATCAACT
CCTTTGGGGTGCGCGAGCTGATGGACGGGATCGGCCAATACGGCCCTGTGCCTCAGATCCAATCCGCCGAACCCCGCCAG
ATCGCGCCCGAAGAGAAAAAAGTCGCAGGCTTTGTCTTTAAGGTGCAGGCCAATATGGACCCAAAGCACCGCGACCGCGT
CGCCTTTGTGCGTCTGGCGTCGGGCCACTTCAAACGTGGCATGAAGCTGACGCATGTGCGCAGCAAGAAACCGATGGCGA
TCTCCAACCCGGTGCTGTTCCTCGCCTCTGACCGCGAGCTTGCAGAAGAGGCCTGGGGCGGCGACATCATCGGCATTCCC
AACCACGGCCAGCTGCGCATTGGCGACACGCTCACCGAAGGCGAGGCGCTGCGCGTCACCGGCATCCCCTCATTCGCACC
GGAGCTGTTGCAAGGCGTGCGTGCGGGCGACCCGATGAAGGCCAAGCACCTTGAAAAAGCCCTGATGCAATTTGCCGAAG
AAGGCGCCGCAAAGGTCTTCAAACCTTCGATCGGCTCGGGCTTTATCGTCGGTGTTGTCGGCGCGCTGCAATTTGAGGTG
CTCGCCAGCCGCATCGAAATGGAATACGGCCTTCCCGTGCGCTTTGAGGCATCGCAGTTCACCTCCGCACGCTGGGTGCA
TGGGGACAAGACGGCAGTGGACAAATTCGTCAACGCCAACAAGCAGCACATCGCCCATGACAACGATGGCGACATCGTCT
ACCTCACGCGGCTGCAATGGGACATCGACCGGGTAGAGCGGGATTATCCCGACTTGAAACTCTCCTCCACCAAGGAGATG
ATGGTCTAA

Upstream 100 bases:

>100_bases
CTGACACCTCGACGGGCACCGACATTTTTTAAGAGTCTCCTGAGTGGTCGACCTCTCCGGGGCGGCCCTTGTTTTGGGTG
CCAGCAACGCTCCGGCCCTG

Downstream 100 bases:

>100_bases
CACCACGGGCGCTCCTCACCGGGCGCCCGTCTTGGTTTCAAAAGGGTTAAGAGACGAGGGCAAGCCTCTGCTATGACAGT
AAAATGGGGGCTTGTCGCCA

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 562; Mature: 562

Protein sequence:

>562_residues
MRPVKHIAPVQHGFSLPLSRFVAKASLMLDTAHNRPDLPPEIARRRTFAIISHPDAGKTTLTEKFLLYGGAIQMAGQVRA
KGEARRTRSDFMQMEKDRGISVSASAMSFDYGDFRYNLVDTPGHSDFSEDTYRTLTAVDAAVMVIDGAKGVESQTQKLFE
VCRLRDLPILTFCNKMDRESRDTFDIIDEIQENLAIDVTPASWPIGVGRDFIGCYDLLRDRLELMDRADRNKVAESISIN
GLDDPKLAEHVPEHLLEKLLEEVEMARELLPTLDPQAVLEGHMTPIWFGSAINSFGVRELMDGIGQYGPVPQIQSAEPRQ
IAPEEKKVAGFVFKVQANMDPKHRDRVAFVRLASGHFKRGMKLTHVRSKKPMAISNPVLFLASDRELAEEAWGGDIIGIP
NHGQLRIGDTLTEGEALRVTGIPSFAPELLQGVRAGDPMKAKHLEKALMQFAEEGAAKVFKPSIGSGFIVGVVGALQFEV
LASRIEMEYGLPVRFEASQFTSARWVHGDKTAVDKFVNANKQHIAHDNDGDIVYLTRLQWDIDRVERDYPDLKLSSTKEM
MV

Sequences:

>Translated_562_residues
MRPVKHIAPVQHGFSLPLSRFVAKASLMLDTAHNRPDLPPEIARRRTFAIISHPDAGKTTLTEKFLLYGGAIQMAGQVRA
KGEARRTRSDFMQMEKDRGISVSASAMSFDYGDFRYNLVDTPGHSDFSEDTYRTLTAVDAAVMVIDGAKGVESQTQKLFE
VCRLRDLPILTFCNKMDRESRDTFDIIDEIQENLAIDVTPASWPIGVGRDFIGCYDLLRDRLELMDRADRNKVAESISIN
GLDDPKLAEHVPEHLLEKLLEEVEMARELLPTLDPQAVLEGHMTPIWFGSAINSFGVRELMDGIGQYGPVPQIQSAEPRQ
IAPEEKKVAGFVFKVQANMDPKHRDRVAFVRLASGHFKRGMKLTHVRSKKPMAISNPVLFLASDRELAEEAWGGDIIGIP
NHGQLRIGDTLTEGEALRVTGIPSFAPELLQGVRAGDPMKAKHLEKALMQFAEEGAAKVFKPSIGSGFIVGVVGALQFEV
LASRIEMEYGLPVRFEASQFTSARWVHGDKTAVDKFVNANKQHIAHDNDGDIVYLTRLQWDIDRVERDYPDLKLSSTKEM
MV
>Mature_562_residues
MRPVKHIAPVQHGFSLPLSRFVAKASLMLDTAHNRPDLPPEIARRRTFAIISHPDAGKTTLTEKFLLYGGAIQMAGQVRA
KGEARRTRSDFMQMEKDRGISVSASAMSFDYGDFRYNLVDTPGHSDFSEDTYRTLTAVDAAVMVIDGAKGVESQTQKLFE
VCRLRDLPILTFCNKMDRESRDTFDIIDEIQENLAIDVTPASWPIGVGRDFIGCYDLLRDRLELMDRADRNKVAESISIN
GLDDPKLAEHVPEHLLEKLLEEVEMARELLPTLDPQAVLEGHMTPIWFGSAINSFGVRELMDGIGQYGPVPQIQSAEPRQ
IAPEEKKVAGFVFKVQANMDPKHRDRVAFVRLASGHFKRGMKLTHVRSKKPMAISNPVLFLASDRELAEEAWGGDIIGIP
NHGQLRIGDTLTEGEALRVTGIPSFAPELLQGVRAGDPMKAKHLEKALMQFAEEGAAKVFKPSIGSGFIVGVVGALQFEV
LASRIEMEYGLPVRFEASQFTSARWVHGDKTAVDKFVNANKQHIAHDNDGDIVYLTRLQWDIDRVERDYPDLKLSSTKEM
MV

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI19923640, Length=499, Percent_Identity=27.6553106212425, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI18390331, Length=477, Percent_Identity=27.2536687631027, Blast_Score=156, Evalue=5e-38,
Organism=Homo sapiens, GI25306283, Length=392, Percent_Identity=28.8265306122449, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI25306287, Length=474, Percent_Identity=27.4261603375527, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI157426893, Length=151, Percent_Identity=31.7880794701987, Blast_Score=89, Evalue=2e-17,
Organism=Homo sapiens, GI94966754, Length=158, Percent_Identity=30.379746835443, Blast_Score=80, Evalue=6e-15,
Organism=Homo sapiens, GI4503483, Length=153, Percent_Identity=34.640522875817, Blast_Score=74, Evalue=5e-13,
Organism=Homo sapiens, GI310132016, Length=137, Percent_Identity=29.9270072992701, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI310110807, Length=137, Percent_Identity=29.9270072992701, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI310123363, Length=137, Percent_Identity=29.9270072992701, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1790835, Length=520, Percent_Identity=47.3076923076923, Blast_Score=519, Evalue=1e-148,
Organism=Escherichia coli, GI1789738, Length=487, Percent_Identity=26.4887063655031, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1788922, Length=174, Percent_Identity=33.9080459770115, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI48994988, Length=137, Percent_Identity=35.036496350365, Blast_Score=89, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=532, Percent_Identity=26.8796992481203, Blast_Score=153, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17556745, Length=181, Percent_Identity=36.4640883977901, Blast_Score=122, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17557151, Length=132, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17506493, Length=186, Percent_Identity=27.4193548387097, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71988819, Length=135, Percent_Identity=31.1111111111111, Blast_Score=69, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI71988811, Length=135, Percent_Identity=31.1111111111111, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25141371, Length=199, Percent_Identity=31.1557788944724, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323098, Length=508, Percent_Identity=26.3779527559055, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6322359, Length=367, Percent_Identity=27.7929155313352, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6323320, Length=215, Percent_Identity=26.5116279069767, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324707, Length=154, Percent_Identity=31.1688311688312, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320593, Length=154, Percent_Identity=31.1688311688312, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24582462, Length=481, Percent_Identity=26.4033264033264, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI221458488, Length=533, Percent_Identity=27.0168855534709, Blast_Score=159, Evalue=7e-39,
Organism=Drosophila melanogaster, GI78706572, Length=193, Percent_Identity=32.1243523316062, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24585711, Length=178, Percent_Identity=28.0898876404494, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24585713, Length=178, Percent_Identity=28.0898876404494, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24585709, Length=178, Percent_Identity=28.0898876404494, Blast_Score=71, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28574573, Length=144, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 62705; Mature: 62705

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPVKHIAPVQHGFSLPLSRFVAKASLMLDTAHNRPDLPPEIARRRTFAIISHPDAGKTT
CCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHCCEEEEEEECCCCCCHH
LTEKFLLYGGAIQMAGQVRAKGEARRTRSDFMQMEKDRGISVSASAMSFDYGDFRYNLVD
HHHHHHHHCCHHHHCCHHHCCCHHHHHHHHHHHHHHCCCCEEEEHHEEECCCCEEEEEEC
TPGHSDFSEDTYRTLTAVDAAVMVIDGAKGVESQTQKLFEVCRLRDLPILTFCNKMDRES
CCCCCCCCHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
RDTFDIIDEIQENLAIDVTPASWPIGVGRDFIGCYDLLRDRLELMDRADRNKVAESISIN
CCHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GLDDPKLAEHVPEHLLEKLLEEVEMARELLPTLDPQAVLEGHMTPIWFGSAINSFGVREL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEECHHHHHCCHHHH
MDGIGQYGPVPQIQSAEPRQIAPEEKKVAGFVFKVQANMDPKHRDRVAFVRLASGHFKRG
HHHHHCCCCCCCCCCCCCCCCCCCHHHHEEEEEEEECCCCCCHHCCEEEEEECCCHHHCC
MKLTHVRSKKPMAISNPVLFLASDRELAEEAWGGDIIGIPNHGQLRIGDTLTEGEALRVT
CEEEEECCCCCCEECCCEEEEECCHHHHHHHCCCCEEECCCCCCEEECCEECCCCEEEEE
GIPSFAPELLQGVRAGDPMKAKHLEKALMQFAEEGAAKVFKPSIGSGFIVGVVGALQFEV
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHEECCCCCCCHHHHHHHHHHHHH
LASRIEMEYGLPVRFEASQFTSARWVHGDKTAVDKFVNANKQHIAHDNDGDIVYLTRLQW
HHHHHHHHCCCCEEEEHHHCCCCEEECCCHHHHHHHHCCCHHHCCCCCCCCEEEEEEEEC
DIDRVERDYPDLKLSSTKEMMV
CHHHHHCCCCCCEECCCHHHCC
>Mature Secondary Structure
MRPVKHIAPVQHGFSLPLSRFVAKASLMLDTAHNRPDLPPEIARRRTFAIISHPDAGKTT
CCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHCCEEEEEEECCCCCCHH
LTEKFLLYGGAIQMAGQVRAKGEARRTRSDFMQMEKDRGISVSASAMSFDYGDFRYNLVD
HHHHHHHHCCHHHHCCHHHCCCHHHHHHHHHHHHHHCCCCEEEEHHEEECCCCEEEEEEC
TPGHSDFSEDTYRTLTAVDAAVMVIDGAKGVESQTQKLFEVCRLRDLPILTFCNKMDRES
CCCCCCCCHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
RDTFDIIDEIQENLAIDVTPASWPIGVGRDFIGCYDLLRDRLELMDRADRNKVAESISIN
CCHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GLDDPKLAEHVPEHLLEKLLEEVEMARELLPTLDPQAVLEGHMTPIWFGSAINSFGVREL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEECHHHHHCCHHHH
MDGIGQYGPVPQIQSAEPRQIAPEEKKVAGFVFKVQANMDPKHRDRVAFVRLASGHFKRG
HHHHHCCCCCCCCCCCCCCCCCCCHHHHEEEEEEEECCCCCCHHCCEEEEEECCCHHHCC
MKLTHVRSKKPMAISNPVLFLASDRELAEEAWGGDIIGIPNHGQLRIGDTLTEGEALRVT
CEEEEECCCCCCEECCCEEEEECCHHHHHHHCCCCEEECCCCCCEEECCEECCCCEEEEE
GIPSFAPELLQGVRAGDPMKAKHLEKALMQFAEEGAAKVFKPSIGSGFIVGVVGALQFEV
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHEECCCCCCCHHHHHHHHHHHHH
LASRIEMEYGLPVRFEASQFTSARWVHGDKTAVDKFVNANKQHIAHDNDGDIVYLTRLQW
HHHHHHHHCCCCEEEEHHHCCCCEEECCCHHHHHHHHCCCHHHCCCCCCCCEEEEEEEEC
DIDRVERDYPDLKLSSTKEMMV
CHHHHHCCCCCCEECCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA