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The map label for this gene is dnaB [H]

Identifier: 99081619

GI number: 99081619

Start: 1871573

End: 1873090

Strand: Direct

Name: dnaB [H]

Synonym: TM1040_1778

Alternate gene names: 99081619

Gene position: 1871573-1873090 (Clockwise)

Preceding gene: 99081618

Following gene: 99081620

Centisome position: 58.47

GC content: 61.46

Gene sequence:

>1518_bases
ATGAACGAGATCACCTCTTTCAACGGCGATCCACAGCCGCCAGCGTCACCGGACGCGCGCGGTGCGGCAGATGAAGCGCA
GGCAACAGAATTGCCCCATTCCGTCGAGGCGGAACAGCAACTGCTGGGGGCAATTCTCACCAACAATGACGTCTATGACA
AAGTCGCCTCGATCATAAATTCCTCGCATTTCTACGACCCGGTCCACGCCCGGATCTACGAGGTGGCCGCGGCCCGGATC
TCCAAGAACGCGCTGGCTTCGCCAGTGACGCTCAAGGCCTTCATGGAGGATGATCAGGGCCTCAAAGAATTGGGCGGCCC
CGCCTATCTCGCCAAGCTGGCCTCGGCGTCGATCTCCGCCTTTGCGGTGCGCGACTATGCACAGATGATTTATGATCTGG
CGATCCGGCGCGAACTCATCGCGCTTGGCCAGACCATCGCGGAAAAAGCCCGCCGCGTCGATGTCGCCTCCGAACCCAAG
GAACAGATCGTCGAGGCCGAACAGTCGCTCTACCAGCTGGCCGAACAGGGCCAGACCGAACAGGGGTTCAAATCCTTCCT
GCGCGCTGTCACCGAGGCCGTGAATGTCACCAATGAGGCCTACCAGCGCGGCGGCGGCATGGCCGGGATCTCTACCGGGC
TGGATGATCTCGACAAACAGCTGGGGGGCTTGCACCCTTCGGACCTGCTGATCCTCGCCGGACGCCCCTCGATGGGGAAA
ACCTCGCTTGCCACCAACATCGCCTTCAACGTCGCCAAAGCCTATCGGCGCGGTCTGAAACAGGACGGCAGCGAAGGGGC
CATTGACGGCGGCGTTGTGGGCTTCTTCTCGCTCGAGATGTCCGCCGAACAGCTGGCAGGCCGGATCCTGGCCGAAGCGT
CAGAGATCTCCTCGCACAAGATCCGCCAGGGTGACATGACCGAAGAAGAGTTCCGCCGGTTCGTCCAAGCCGCCAAGGAT
CTGGAAAGCTGCCCGCTCTTTATCGACGATACGCCCGCGATCCCGATCTCACAGCTGGCCGCCCGCGCCCGCCGCCTGAA
ACGGACCCATGGGCTCGACCTTCTGGTGATCGACTACCTGCAGCTCTGCCGCGGCATGTCCGACAACCGCGTGAACGAGA
TTGCCGAGATCTCGATGGGCATGAAAGCCATCGCCAAGGAACTCAACATCCCCGTCATCGCCCTGTCGCAGCTCTCCCGT
CAGGTCGAAAGCCGCGAGGACAAACGCCCGCAGCTCTCCGACCTGCGGGAATCGGGCTCGATCGAACAGGACGCCGACGT
GGTCATGTTCGTGTTCCGCGAAGAATACTACAAAGAACGCGAAAAACCGGGCGACCACGAGCTCGACAAGATGGAAGAGT
GGAAACAGGCGATGGAACGCTTGCACGCGAAGGCCGAAGTCATCGTCGGCAAACAGCGTCACGGCCCCATTGGCACGGTC
GAACTTTCGTTCGAGGCGCAGTTCACGCGCTTTGGCAACCTCGCCAAAGCCTGGCAGCAGCAACCCGACGGCTATTAA

Upstream 100 bases:

>100_bases
TCGACCTCTGCGGTTATCCTCAAACGATCCACAGGGATTTCCAGATGGTATTCGGGGCAGTTTCCAGCGTAAAACACGAG
CCTATCAAAAGAGAGCAATC

Downstream 100 bases:

>100_bases
GGTCACAAAAGGGGCGCCGGGCCACCGGTCCCCTTCATCTTTTCCTCAATACCTCGGGGGAGGCCCATCAGGCCGGGGCA
GCGTCCCCACCTGTGGGTGC

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MNEITSFNGDPQPPASPDARGAADEAQATELPHSVEAEQQLLGAILTNNDVYDKVASIINSSHFYDPVHARIYEVAAARI
SKNALASPVTLKAFMEDDQGLKELGGPAYLAKLASASISAFAVRDYAQMIYDLAIRRELIALGQTIAEKARRVDVASEPK
EQIVEAEQSLYQLAEQGQTEQGFKSFLRAVTEAVNVTNEAYQRGGGMAGISTGLDDLDKQLGGLHPSDLLILAGRPSMGK
TSLATNIAFNVAKAYRRGLKQDGSEGAIDGGVVGFFSLEMSAEQLAGRILAEASEISSHKIRQGDMTEEEFRRFVQAAKD
LESCPLFIDDTPAIPISQLAARARRLKRTHGLDLLVIDYLQLCRGMSDNRVNEIAEISMGMKAIAKELNIPVIALSQLSR
QVESREDKRPQLSDLRESGSIEQDADVVMFVFREEYYKEREKPGDHELDKMEEWKQAMERLHAKAEVIVGKQRHGPIGTV
ELSFEAQFTRFGNLAKAWQQQPDGY

Sequences:

>Translated_505_residues
MNEITSFNGDPQPPASPDARGAADEAQATELPHSVEAEQQLLGAILTNNDVYDKVASIINSSHFYDPVHARIYEVAAARI
SKNALASPVTLKAFMEDDQGLKELGGPAYLAKLASASISAFAVRDYAQMIYDLAIRRELIALGQTIAEKARRVDVASEPK
EQIVEAEQSLYQLAEQGQTEQGFKSFLRAVTEAVNVTNEAYQRGGGMAGISTGLDDLDKQLGGLHPSDLLILAGRPSMGK
TSLATNIAFNVAKAYRRGLKQDGSEGAIDGGVVGFFSLEMSAEQLAGRILAEASEISSHKIRQGDMTEEEFRRFVQAAKD
LESCPLFIDDTPAIPISQLAARARRLKRTHGLDLLVIDYLQLCRGMSDNRVNEIAEISMGMKAIAKELNIPVIALSQLSR
QVESREDKRPQLSDLRESGSIEQDADVVMFVFREEYYKEREKPGDHELDKMEEWKQAMERLHAKAEVIVGKQRHGPIGTV
ELSFEAQFTRFGNLAKAWQQQPDGY
>Mature_505_residues
MNEITSFNGDPQPPASPDARGAADEAQATELPHSVEAEQQLLGAILTNNDVYDKVASIINSSHFYDPVHARIYEVAAARI
SKNALASPVTLKAFMEDDQGLKELGGPAYLAKLASASISAFAVRDYAQMIYDLAIRRELIALGQTIAEKARRVDVASEPK
EQIVEAEQSLYQLAEQGQTEQGFKSFLRAVTEAVNVTNEAYQRGGGMAGISTGLDDLDKQLGGLHPSDLLILAGRPSMGK
TSLATNIAFNVAKAYRRGLKQDGSEGAIDGGVVGFFSLEMSAEQLAGRILAEASEISSHKIRQGDMTEEEFRRFVQAAKD
LESCPLFIDDTPAIPISQLAARARRLKRTHGLDLLVIDYLQLCRGMSDNRVNEIAEISMGMKAIAKELNIPVIALSQLSR
QVESREDKRPQLSDLRESGSIEQDADVVMFVFREEYYKEREKPGDHELDKMEEWKQAMERLHAKAEVIVGKQRHGPIGTV
ELSFEAQFTRFGNLAKAWQQQPDGY

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=471, Percent_Identity=40.1273885350318, Blast_Score=313, Evalue=2e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 55600; Mature: 55600

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEITSFNGDPQPPASPDARGAADEAQATELPHSVEAEQQLLGAILTNNDVYDKVASIIN
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
SSHFYDPVHARIYEVAAARISKNALASPVTLKAFMEDDQGLKELGGPAYLAKLASASISA
CCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHH
FAVRDYAQMIYDLAIRRELIALGQTIAEKARRVDVASEPKEQIVEAEQSLYQLAEQGQTE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCH
QGFKSFLRAVTEAVNVTNEAYQRGGGMAGISTGLDDLDKQLGGLHPSDLLILAGRPSMGK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHCCCCCCCEEEEECCCCCCC
TSLATNIAFNVAKAYRRGLKQDGSEGAIDGGVVGFFSLEMSAEQLAGRILAEASEISSHK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
IRQGDMTEEEFRRFVQAAKDLESCPLFIDDTPAIPISQLAARARRLKRTHGLDLLVIDYL
CCCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHH
QLCRGMSDNRVNEIAEISMGMKAIAKELNIPVIALSQLSRQVESREDKRPQLSDLRESGS
HHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCC
IEQDADVVMFVFREEYYKEREKPGDHELDKMEEWKQAMERLHAKAEVIVGKQRHGPIGTV
CCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
ELSFEAQFTRFGNLAKAWQQQPDGY
EEEECHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNEITSFNGDPQPPASPDARGAADEAQATELPHSVEAEQQLLGAILTNNDVYDKVASIIN
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
SSHFYDPVHARIYEVAAARISKNALASPVTLKAFMEDDQGLKELGGPAYLAKLASASISA
CCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHH
FAVRDYAQMIYDLAIRRELIALGQTIAEKARRVDVASEPKEQIVEAEQSLYQLAEQGQTE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCH
QGFKSFLRAVTEAVNVTNEAYQRGGGMAGISTGLDDLDKQLGGLHPSDLLILAGRPSMGK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHCCCCCCCEEEEECCCCCCC
TSLATNIAFNVAKAYRRGLKQDGSEGAIDGGVVGFFSLEMSAEQLAGRILAEASEISSHK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
IRQGDMTEEEFRRFVQAAKDLESCPLFIDDTPAIPISQLAARARRLKRTHGLDLLVIDYL
CCCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHH
QLCRGMSDNRVNEIAEISMGMKAIAKELNIPVIALSQLSRQVESREDKRPQLSDLRESGS
HHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCC
IEQDADVVMFVFREEYYKEREKPGDHELDKMEEWKQAMERLHAKAEVIVGKQRHGPIGTV
CCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
ELSFEAQFTRFGNLAKAWQQQPDGY
EEEECHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA