Click here to switch to the map view.

The map label for this gene is metR [H]

Identifier: 99081540

GI number: 99081540

Start: 1795432

End: 1796337

Strand: Reverse

Name: metR [H]

Synonym: TM1040_1699

Alternate gene names: 99081540

Gene position: 1796337-1795432 (Counterclockwise)

Preceding gene: 99081543

Following gene: 99081539

Centisome position: 56.12

GC content: 60.38

Gene sequence:

>906_bases
ATGCACATCGAGTTTCGTCATCTTCGGACCATCAAGGCGATCCACGAGGCCGGTGGGCTGGCGCGGGCGGCGGATCAGCT
CAACATCACCCAGAGCGCGCTCAGTCATCAGATCAAGGGGCTTGAAGAGCAGGCGGGGGTAGAGCTGTTTTTGCGCCGCT
CAAAACCGATGAAGCTCTCTGCGGCGGGTCTGCGGCTGTTGCGGTTGGCAGAGCAGATCCTGCCGCAAGTTGAGTCTGCG
CAAGCAGAATTTGCCGCCCTGCGCGAGGGCGAGGCGGGGCGTCTGCACATCGCAATTGAATGCCACGCCTGTTTTGAATG
GCTGTTTCCTGTCCTGGAGGGCTTTCGCAAAAGCTGGGGGGATGTGGATGTGGACATCCGTCCGGGTCTCGCGTTCGAGG
CCATGCCCGCTTTGATGCGCGAAGAGGTGGATCTGGTGGTGACCTCCGACCCCGAGGACATGCCGGGGGTCTCCTTTATT
GAACTCTTTGATTACAATCCCGTGTTTGTCGCCTCTGCCCAGCACGCGCTTGCCGCAAAGCCCTATGTAGAAGCCGAGGA
TTTTCGTGGTCAGACCCTCATTACCTATCCCGTAGAGCGCACCCGCCTCGATGTGTTCAGCCAGCTCTTGATCCCGGCGG
GCGTGGAGCCTGCGAGCATCAGGAAGGTCGAACTGACGGCGGTGATCTTGTTGTTGGTGGCCTCCAACCGCGGGGTCTCG
GTGTTGCCTGACTGGGTTGTGCGCGAGGTGAAGTATTCCTCGGATTATGTGACGCGCCCGCTGACAAAACTGGGCATCAC
CCGCCGGCTTTATGCAGCGATCCGCAGCGAGGACGAAAGCAAACCGTTCATGCAGGAGCTGATCCGTCTGGCCCGGGTCG
AGGCGCGCAAGTTGCAGCAGGTCTAG

Upstream 100 bases:

>100_bases
TGCGACACATGTGTCGCTTCAGGTTTGACCCCTTTTCACATGAGAGCTATCAGTGAATCAAATTCATATTTCTCAAGAAC
AACATGAGGCAGATCGCAGT

Downstream 100 bases:

>100_bases
GGTGGTGGGCGTTGGTCCAATGGTGGCTCAGGGCCCGCACAAGGTGGGGGTAGCGGCTCTTATGCACTGGGTGCATGGCT
GAAACTTAACCGCAAAGGGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MHIEFRHLRTIKAIHEAGGLARAADQLNITQSALSHQIKGLEEQAGVELFLRRSKPMKLSAAGLRLLRLAEQILPQVESA
QAEFAALREGEAGRLHIAIECHACFEWLFPVLEGFRKSWGDVDVDIRPGLAFEAMPALMREEVDLVVTSDPEDMPGVSFI
ELFDYNPVFVASAQHALAAKPYVEAEDFRGQTLITYPVERTRLDVFSQLLIPAGVEPASIRKVELTAVILLLVASNRGVS
VLPDWVVREVKYSSDYVTRPLTKLGITRRLYAAIRSEDESKPFMQELIRLARVEARKLQQV

Sequences:

>Translated_301_residues
MHIEFRHLRTIKAIHEAGGLARAADQLNITQSALSHQIKGLEEQAGVELFLRRSKPMKLSAAGLRLLRLAEQILPQVESA
QAEFAALREGEAGRLHIAIECHACFEWLFPVLEGFRKSWGDVDVDIRPGLAFEAMPALMREEVDLVVTSDPEDMPGVSFI
ELFDYNPVFVASAQHALAAKPYVEAEDFRGQTLITYPVERTRLDVFSQLLIPAGVEPASIRKVELTAVILLLVASNRGVS
VLPDWVVREVKYSSDYVTRPLTKLGITRRLYAAIRSEDESKPFMQELIRLARVEARKLQQV
>Mature_301_residues
MHIEFRHLRTIKAIHEAGGLARAADQLNITQSALSHQIKGLEEQAGVELFLRRSKPMKLSAAGLRLLRLAEQILPQVESA
QAEFAALREGEAGRLHIAIECHACFEWLFPVLEGFRKSWGDVDVDIRPGLAFEAMPALMREEVDLVVTSDPEDMPGVSFI
ELFDYNPVFVASAQHALAAKPYVEAEDFRGQTLITYPVERTRLDVFSQLLIPAGVEPASIRKVELTAVILLLVASNRGVS
VLPDWVVREVKYSSDYVTRPLTKLGITRRLYAAIRSEDESKPFMQELIRLARVEARKLQQV

Specific function: Control of the last step in methionine biosynthesis; metR is a positive activator of the metA, metE and metH genes. It is also a negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790262, Length=293, Percent_Identity=43.0034129692833, Blast_Score=243, Evalue=1e-65,
Organism=Escherichia coli, GI2367136, Length=242, Percent_Identity=27.6859504132231, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1787879, Length=250, Percent_Identity=31.6, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788887, Length=300, Percent_Identity=28, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790208, Length=172, Percent_Identity=31.3953488372093, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI145693105, Length=278, Percent_Identity=24.8201438848921, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI157672245, Length=241, Percent_Identity=24.0663900414938, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789173, Length=187, Percent_Identity=27.807486631016, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1789204, Length=242, Percent_Identity=25.6198347107438, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787530, Length=301, Percent_Identity=21.9269102990033, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1786401, Length=135, Percent_Identity=28.1481481481481, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1786713, Length=93, Percent_Identity=37.6344086021505, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787601, Length=305, Percent_Identity=23.9344262295082, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33766; Mature: 33766

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIEFRHLRTIKAIHEAGGLARAADQLNITQSALSHQIKGLEEQAGVELFLRRSKPMKLS
CCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHEEECCCCCEEH
AAGLRLLRLAEQILPQVESAQAEFAALREGEAGRLHIAIECHACFEWLFPVLEGFRKSWG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCC
DVDVDIRPGLAFEAMPALMREEVDLVVTSDPEDMPGVSFIELFDYNPVFVASAQHALAAK
CEEEEECCCCHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECCCCEEEECCCHHHHCC
PYVEAEDFRGQTLITYPVERTRLDVFSQLLIPAGVEPASIRKVELTAVILLLVASNRGVS
CCCCHHCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
VLPDWVVREVKYSSDYVTRPLTKLGITRRLYAAIRSEDESKPFMQELIRLARVEARKLQQ
CCHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
V
C
>Mature Secondary Structure
MHIEFRHLRTIKAIHEAGGLARAADQLNITQSALSHQIKGLEEQAGVELFLRRSKPMKLS
CCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHEEECCCCCEEH
AAGLRLLRLAEQILPQVESAQAEFAALREGEAGRLHIAIECHACFEWLFPVLEGFRKSWG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCC
DVDVDIRPGLAFEAMPALMREEVDLVVTSDPEDMPGVSFIELFDYNPVFVASAQHALAAK
CEEEEECCCCHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECCCCEEEECCCHHHHCC
PYVEAEDFRGQTLITYPVERTRLDVFSQLLIPAGVEPASIRKVELTAVILLLVASNRGVS
CCCCHHCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
VLPDWVVREVKYSSDYVTRPLTKLGITRRLYAAIRSEDESKPFMQELIRLARVEARKLQQ
CCHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
V
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]