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The map label for this gene is yeiT [H]

Identifier: 99081342

GI number: 99081342

Start: 1607447

End: 1608781

Strand: Reverse

Name: yeiT [H]

Synonym: TM1040_1501

Alternate gene names: 99081342

Gene position: 1608781-1607447 (Counterclockwise)

Preceding gene: 99081343

Following gene: 99081341

Centisome position: 50.26

GC content: 62.32

Gene sequence:

>1335_bases
ATGGCAGTAGGCCACCAGGTATCCGGCGTGCGTTCTGAGCGATTGAGTGCCCAGGAAATCGCCGAGAACTTCGCAGATCT
TCACGCGCCTTATGACGCGCATGAAGCCGCGGTTGCAGCGGATCGCTGCTATTTCTGTTACGACGCGCCCTGCATGACGG
CCTGTCCGACAAGCATCGACATCCCGCAGTTCATTCGTGAAATTCAGGCGGGTCATGCCGAGAGCGCGGCAAAGACCATT
CTTGAGCAGAACATCCTTGGCGGGATGTGTGCGCGGGTTTGTCCGACAGAGACGCTCTGCGAGGAAGTCTGCGTGCGTGA
AGTGGCCGAAGGCAAGCCGGTCGAGATCGGTCGCCTTCAGCGCTATGCCACGGATATGCTGATGGAAAAGGGCGCGCACC
CCTTTACCCGTGCTGCGGCGACAGGCAAGCGGGTCGCGGTCGTCGGTGCGGGGCCTGCCGGGCTTGCGGCGGCGCACCGG
TTGGCTATGCACGGTCATGAGGTGACAATCTACGAGTCCAAACCCAAGGCGGGTGGTCTCAATGAATTTGGCATCGCCGC
CTATAAATCGACCAATGATTTTGCTGCGCGCGAGGTCGAGTGGCTGCTCCAGATCGGTGGGATCACCATCGAGTACGGTC
AGGGCCTCGGCGCGGGCCTCACGCTTGACGGGCTTCAGGCGGATTTCGACGCGGTGTTCCTGTCTGTCGGGCTGCAAGGC
GTCAACGCGCTGCGCGCAGAAGGTGAAGACAAGGACGGCGTGCGCGATGCGGTGGATTTCATTGCCGAACTGCGCCAGGC
CGAAGATCTGCGCTCGCTTCCGGTTGGGCGCAATGTGGTGGTGATCGGCGGCGGTATGACCGCTGTAGACGCGGCCGTGC
AGTCCAAACTCTTGGGTGCCGAGAATGTGACCATCGCTTATCGTCGCGGTCGCGACGCCATGGGCGCAAGCCGTTTTGAG
CAGGATCTCGCGGCCTCCAAGGGCGTGAAACTGATGTTCAACGTGATGCCCAAGGCGATCCATGGCAACGGCGCCGCCGC
AGAGATCGAGCTCGAGTACACCGAAATCGTTGACGGGCGCGTGAAAGGCACCGGAGAGACCCTCCGCCTTGCTGCGGATC
AGGTGATGAAAGCCATCGGTCAGACGCTCGAAGGCCAGCCGGATGCACTGGCGCTTGAGGGCGGCAAGATCAAAGTCTCC
GAGACGGGCAAAACCTCCGTCGCCGGGGTCTGGGCCGGGGGCGACTGTGCCTCTGGCGGCGATGATCTCACGGTCACTGC
CGTTGCCGAGGGTCGCGATGCGGCCGAAGATATCCACGCAAGCCTGATGGGCTGA

Upstream 100 bases:

>100_bases
CCTCTCTAAATTTTACCAGTTGATAAAAAAATCGCCTGTGGCACTGTGTGGATAACAAGAAGACATCACTGACTGTCCAA
TTTCAGCAAGGAGACACCCA

Downstream 100 bases:

>100_bases
AGCCGCTATGCGCCGCTCCCCCAGGTGGGGCGGCTTGCATAGTTAGCAAATAGGGAGAGATCAAATGGCTGATCTTACAA
CAGAATTCCTCGGTATCAAA

Product: putative oxidoreductase

Products: L-glutamine; 2-oxoglutarate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MAVGHQVSGVRSERLSAQEIAENFADLHAPYDAHEAAVAADRCYFCYDAPCMTACPTSIDIPQFIREIQAGHAESAAKTI
LEQNILGGMCARVCPTETLCEEVCVREVAEGKPVEIGRLQRYATDMLMEKGAHPFTRAAATGKRVAVVGAGPAGLAAAHR
LAMHGHEVTIYESKPKAGGLNEFGIAAYKSTNDFAAREVEWLLQIGGITIEYGQGLGAGLTLDGLQADFDAVFLSVGLQG
VNALRAEGEDKDGVRDAVDFIAELRQAEDLRSLPVGRNVVVIGGGMTAVDAAVQSKLLGAENVTIAYRRGRDAMGASRFE
QDLAASKGVKLMFNVMPKAIHGNGAAAEIELEYTEIVDGRVKGTGETLRLAADQVMKAIGQTLEGQPDALALEGGKIKVS
ETGKTSVAGVWAGGDCASGGDDLTVTAVAEGRDAAEDIHASLMG

Sequences:

>Translated_444_residues
MAVGHQVSGVRSERLSAQEIAENFADLHAPYDAHEAAVAADRCYFCYDAPCMTACPTSIDIPQFIREIQAGHAESAAKTI
LEQNILGGMCARVCPTETLCEEVCVREVAEGKPVEIGRLQRYATDMLMEKGAHPFTRAAATGKRVAVVGAGPAGLAAAHR
LAMHGHEVTIYESKPKAGGLNEFGIAAYKSTNDFAAREVEWLLQIGGITIEYGQGLGAGLTLDGLQADFDAVFLSVGLQG
VNALRAEGEDKDGVRDAVDFIAELRQAEDLRSLPVGRNVVVIGGGMTAVDAAVQSKLLGAENVTIAYRRGRDAMGASRFE
QDLAASKGVKLMFNVMPKAIHGNGAAAEIELEYTEIVDGRVKGTGETLRLAADQVMKAIGQTLEGQPDALALEGGKIKVS
ETGKTSVAGVWAGGDCASGGDDLTVTAVAEGRDAAEDIHASLMG
>Mature_443_residues
AVGHQVSGVRSERLSAQEIAENFADLHAPYDAHEAAVAADRCYFCYDAPCMTACPTSIDIPQFIREIQAGHAESAAKTIL
EQNILGGMCARVCPTETLCEEVCVREVAEGKPVEIGRLQRYATDMLMEKGAHPFTRAAATGKRVAVVGAGPAGLAAAHRL
AMHGHEVTIYESKPKAGGLNEFGIAAYKSTNDFAAREVEWLLQIGGITIEYGQGLGAGLTLDGLQADFDAVFLSVGLQGV
NALRAEGEDKDGVRDAVDFIAELRQAEDLRSLPVGRNVVVIGGGMTAVDAAVQSKLLGAENVTIAYRRGRDAMGASRFEQ
DLAASKGVKLMFNVMPKAIHGNGAAAEIELEYTEIVDGRVKGTGETLRLAADQVMKAIGQTLEGQPDALALEGGKIKVSE
TGKTSVAGVWAGGDCASGGDDLTVTAVAEGRDAAEDIHASLMG

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli glutamate synthase [NADPH] small chain (GltD) and to E.coli AegA [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=457, Percent_Identity=30.6345733041575, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI237757300, Length=115, Percent_Identity=39.1304347826087, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1788468, Length=426, Percent_Identity=36.8544600938967, Blast_Score=221, Evalue=8e-59,
Organism=Escherichia coli, GI1789606, Length=450, Percent_Identity=35.5555555555556, Blast_Score=215, Evalue=6e-57,
Organism=Escherichia coli, GI1788811, Length=457, Percent_Identity=35.0109409190372, Blast_Score=214, Evalue=8e-57,
Organism=Escherichia coli, GI87082180, Length=458, Percent_Identity=34.061135371179, Blast_Score=204, Evalue=6e-54,
Organism=Escherichia coli, GI1789243, Length=411, Percent_Identity=29.683698296837, Blast_Score=124, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71984108, Length=452, Percent_Identity=31.4159292035398, Blast_Score=161, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17570289, Length=452, Percent_Identity=28.3185840707965, Blast_Score=116, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6320030, Length=291, Percent_Identity=29.553264604811, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24640763, Length=453, Percent_Identity=34.2163355408389, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI18858217, Length=453, Percent_Identity=34.2163355408389, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24665547, Length=457, Percent_Identity=30.1969365426696, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24665543, Length=457, Percent_Identity=30.1969365426696, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI28574881, Length=457, Percent_Identity=30.1969365426696, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24665539, Length=457, Percent_Identity=30.1969365426696, Blast_Score=142, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.4.1.13

Molecular weight: Translated: 46557; Mature: 46426

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVGHQVSGVRSERLSAQEIAENFADLHAPYDAHEAAVAADRCYFCYDAPCMTACPTSID
CCCCCCCCCHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEECCCHHHCCCCCCC
IPQFIREIQAGHAESAAKTILEQNILGGMCARVCPTETLCEEVCVREVAEGKPVEIGRLQ
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHH
RYATDMLMEKGAHPFTRAAATGKRVAVVGAGPAGLAAAHRLAMHGHEVTIYESKPKAGGL
HHHHHHHHHCCCCCHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCC
NEFGIAAYKSTNDFAAREVEWLLQIGGITIEYGQGLGAGLTLDGLQADFDAVFLSVGLQG
CCCCEEEECCCCCHHHHHHHHHHHHCCEEEECCCCCCCCEEECCCHHHHHHHHHHHHHHH
VNALRAEGEDKDGVRDAVDFIAELRQAEDLRSLPVGRNVVVIGGGMTAVDAAVQSKLLGA
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHCCC
ENVTIAYRRGRDAMGASRFEQDLAASKGVKLMFNVMPKAIHGNGAAAEIELEYTEIVDGR
CCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCHHCCCCCCEEEEEEEHHHHCCCE
VKGTGETLRLAADQVMKAIGQTLEGQPDALALEGGKIKVSETGKTSVAGVWAGGDCASGG
ECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEECCCCCEEEEEEECCCCCCCC
DDLTVTAVAEGRDAAEDIHASLMG
CCEEEEEEECCCCHHHHHHHHHCC
>Mature Secondary Structure 
AVGHQVSGVRSERLSAQEIAENFADLHAPYDAHEAAVAADRCYFCYDAPCMTACPTSID
CCCCCCCCHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEECCCHHHCCCCCCC
IPQFIREIQAGHAESAAKTILEQNILGGMCARVCPTETLCEEVCVREVAEGKPVEIGRLQ
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHH
RYATDMLMEKGAHPFTRAAATGKRVAVVGAGPAGLAAAHRLAMHGHEVTIYESKPKAGGL
HHHHHHHHHCCCCCHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCC
NEFGIAAYKSTNDFAAREVEWLLQIGGITIEYGQGLGAGLTLDGLQADFDAVFLSVGLQG
CCCCEEEECCCCCHHHHHHHHHHHHCCEEEECCCCCCCCEEECCCHHHHHHHHHHHHHHH
VNALRAEGEDKDGVRDAVDFIAELRQAEDLRSLPVGRNVVVIGGGMTAVDAAVQSKLLGA
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHCCC
ENVTIAYRRGRDAMGASRFEQDLAASKGVKLMFNVMPKAIHGNGAAAEIELEYTEIVDGR
CCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCHHCCCCCCEEEEEEEHHHHCCCE
VKGTGETLRLAADQVMKAIGQTLEGQPDALALEGGKIKVSETGKTSVAGVWAGGDCASGG
ECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEECCCCCEEEEEEECCCCCCCC
DDLTVTAVAEGRDAAEDIHASLMG
CCEEEEEEECCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; NADP+

Specific reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]