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The map label for this gene is metA

Identifier: 99081220

GI number: 99081220

Start: 1473238

End: 1474176

Strand: Direct

Name: metA

Synonym: TM1040_1379

Alternate gene names: 99081220

Gene position: 1473238-1474176 (Clockwise)

Preceding gene: 99081219

Following gene: 99081221

Centisome position: 46.03

GC content: 57.93

Gene sequence:

>939_bases
ATGCCTATCAAGATCCCCGCTGACCTGCCCGCCTACTCCGTCCTGTCGGACGAGGGCGTCATGGTCATGTCGCCGGATCA
AGCGGCGCGTCAGGACATCCGACCGATCAAGATTGGCCTGCTCAACTTGATGCCGAAGAAAATTCAGACGGAAAACCAGT
TTGCCCGTCTGATTGGGGCGACGCCGCTGCAGATCGAGTTCTCCTTGATCCGCATGACCGAGCATCAGACCAAGAACACC
GCCGCCGCCCACATGGAAGAGTTTTATCGCCCCTTTCAGGAGGTGCGCGACGAGAAATTCGATGGTCTGATCATCACCGG
TGCTCCGATTGAGCATCTGCCGTTCGAGGACGTCACCTATTGGCAGGAGCTCTGCGAGGTCTTTGAGTGGACGCAGACCA
ATGTGCATTCCACCTTTGGCGTTTGCTGGGGCGGCATGGCGATGATCAATTACTTTCACGGCGTGCGCAAACATATGCTG
GATCACAAGGCCTTTGGCTGTTTCCGGCATCAGAACCTGCAGCCGACCTCGCCCTATCTGCGCGGGTTTTCGGATGATTT
TGTGATCCCCGTGTCGCGCTGGACCGAAATGAGCCAGAGCGAGATCGACGCCGCACCGGGTCTGACCACGCTCTTGGGCA
GCGCCGATGTCGGCCCCTGCCTCGTTGAGGACAAGGCGCACCGTGCGCTCTATATCTTCAACCACTTTGAATATGACAGC
GACACGCTGAAACAAGAATATGACCGAGATGTCGCCAGCGGCACCGAGATCAACGTGCCGATCAACTACTACCCGGACGA
CGATCCAAGCCAGCGCCCGCTCAACCGTTGGCGCAGCCACGCGCATCTGCTCTATGGCAACTGGGTGTCTGAAATCTATC
AGACGACCCCATATGATGTGGAGCGCATCGGTCTCGACACGACGGACCTTCGGGCCTGA

Upstream 100 bases:

>100_bases
CGATTTCGAGGAGTTGATTGCATCCGCTCTTGAGACACGAGCGGAACAGACTTAATTAACGGCACCCTGCTACTTTTAGC
GCACCCACAAAGGACACGAC

Downstream 100 bases:

>100_bases
TCTTTGCGGATGAGTGCCATCCTTGCTCTTGCCGTGACGCTTGGCTTTGCTGGGGTTGCGGGCTTTACCGCCCTGCGCGC
CCCACTGCGCGAGGCCGAGA

Product: homoserine O-succinyltransferase

Products: NA

Alternate protein names: Homoserine O-transsuccinylase; HTS

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MPIKIPADLPAYSVLSDEGVMVMSPDQAARQDIRPIKIGLLNLMPKKIQTENQFARLIGATPLQIEFSLIRMTEHQTKNT
AAAHMEEFYRPFQEVRDEKFDGLIITGAPIEHLPFEDVTYWQELCEVFEWTQTNVHSTFGVCWGGMAMINYFHGVRKHML
DHKAFGCFRHQNLQPTSPYLRGFSDDFVIPVSRWTEMSQSEIDAAPGLTTLLGSADVGPCLVEDKAHRALYIFNHFEYDS
DTLKQEYDRDVASGTEINVPINYYPDDDPSQRPLNRWRSHAHLLYGNWVSEIYQTTPYDVERIGLDTTDLRA

Sequences:

>Translated_312_residues
MPIKIPADLPAYSVLSDEGVMVMSPDQAARQDIRPIKIGLLNLMPKKIQTENQFARLIGATPLQIEFSLIRMTEHQTKNT
AAAHMEEFYRPFQEVRDEKFDGLIITGAPIEHLPFEDVTYWQELCEVFEWTQTNVHSTFGVCWGGMAMINYFHGVRKHML
DHKAFGCFRHQNLQPTSPYLRGFSDDFVIPVSRWTEMSQSEIDAAPGLTTLLGSADVGPCLVEDKAHRALYIFNHFEYDS
DTLKQEYDRDVASGTEINVPINYYPDDDPSQRPLNRWRSHAHLLYGNWVSEIYQTTPYDVERIGLDTTDLRA
>Mature_311_residues
PIKIPADLPAYSVLSDEGVMVMSPDQAARQDIRPIKIGLLNLMPKKIQTENQFARLIGATPLQIEFSLIRMTEHQTKNTA
AAHMEEFYRPFQEVRDEKFDGLIITGAPIEHLPFEDVTYWQELCEVFEWTQTNVHSTFGVCWGGMAMINYFHGVRKHMLD
HKAFGCFRHQNLQPTSPYLRGFSDDFVIPVSRWTEMSQSEIDAAPGLTTLLGSADVGPCLVEDKAHRALYIFNHFEYDSD
TLKQEYDRDVASGTEINVPINYYPDDDPSQRPLNRWRSHAHLLYGNWVSEIYQTTPYDVERIGLDTTDLRA

Specific function: Methionine biosynthesis; HTS variant; first step. [C]

COG id: COG1897

COG function: function code E; Homoserine trans-succinylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HTS family

Homologues:

Organism=Escherichia coli, GI1790443, Length=305, Percent_Identity=41.3114754098361, Blast_Score=268, Evalue=3e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): META_SILST (Q1GGV4)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_613374.1
- ProteinModelPortal:   Q1GGV4
- SMR:   Q1GGV4
- STRING:   Q1GGV4
- GeneID:   4075872
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_1379
- NMPDR:   fig|292414.1.peg.3514
- eggNOG:   COG1897
- HOGENOM:   HBG362506
- OMA:   QAGLYYH
- PhylomeDB:   Q1GGV4
- ProtClustDB:   PRK05368
- BioCyc:   SSP292414:TM1040_1379-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00295
- InterPro:   IPR005697
- PANTHER:   PTHR20919
- PIRSF:   PIRSF000450
- ProDom:   PD037892
- TIGRFAMs:   TIGR01001

Pfam domain/function: PF04204 HTS

EC number: =2.3.1.46

Molecular weight: Translated: 35834; Mature: 35703

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: ACT_SITE 142-142

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIKIPADLPAYSVLSDEGVMVMSPDQAARQDIRPIKIGLLNLMPKKIQTENQFARLIGA
CCEECCCCCCCHHEECCCCEEEECCCHHHHHCCCHHHHHHHHHCHHHHCCHHHHHHHHCC
TPLQIEFSLIRMTEHQTKNTAAAHMEEFYRPFQEVRDEKFDGLIITGAPIEHLPFEDVTY
CCEEEEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHH
WQELCEVFEWTQTNVHSTFGVCWGGMAMINYFHGVRKHMLDHKAFGCFRHQNLQPTSPYL
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
RGFSDDFVIPVSRWTEMSQSEIDAAPGLTTLLGSADVGPCLVEDKAHRALYIFNHFEYDS
CCCCCCEEEEHHHHHHHHHHHHCCCCCHHHHHCCCCCCCEEECCCCCCEEEEEEECCCCH
DTLKQEYDRDVASGTEINVPINYYPDDDPSQRPLNRWRSHAHLLYGNWVSEIYQTTPYDV
HHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHCEEEEHHHHHHHHHCCCCCH
ERIGLDTTDLRA
HHCCCCHHHCCC
>Mature Secondary Structure 
PIKIPADLPAYSVLSDEGVMVMSPDQAARQDIRPIKIGLLNLMPKKIQTENQFARLIGA
CEECCCCCCCHHEECCCCEEEECCCHHHHHCCCHHHHHHHHHCHHHHCCHHHHHHHHCC
TPLQIEFSLIRMTEHQTKNTAAAHMEEFYRPFQEVRDEKFDGLIITGAPIEHLPFEDVTY
CCEEEEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHH
WQELCEVFEWTQTNVHSTFGVCWGGMAMINYFHGVRKHMLDHKAFGCFRHQNLQPTSPYL
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
RGFSDDFVIPVSRWTEMSQSEIDAAPGLTTLLGSADVGPCLVEDKAHRALYIFNHFEYDS
CCCCCCEEEEHHHHHHHHHHHHCCCCCHHHHHCCCCCCCEEECCCCCCEEEEEEECCCCH
DTLKQEYDRDVASGTEINVPINYYPDDDPSQRPLNRWRSHAHLLYGNWVSEIYQTTPYDV
HHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHCEEEEHHHHHHHHHCCCCCH
ERIGLDTTDLRA
HHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA