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The map label for this gene is ntrX [H]

Identifier: 99081200

GI number: 99081200

Start: 1451445

End: 1452863

Strand: Reverse

Name: ntrX [H]

Synonym: TM1040_1359

Alternate gene names: 99081200

Gene position: 1452863-1451445 (Counterclockwise)

Preceding gene: 99081201

Following gene: 99081199

Centisome position: 45.39

GC content: 58.99

Gene sequence:

>1419_bases
ATGAGTGATATTCTGATTGTTGATGATGAACGTGACATCCGGGAGCTGGTTTCAGACATCCTGGAGGACGAAAGCTATGC
CACGCGCAAAGCGGCCAACTCCGAAGAATGCATGGCCGCCTTTGAAGACGCTCCGCCTGCGCTGCTGATTTTGGACATCT
GGCTCAAAGACAGCCAGATGGATGGGATCGACATCCTGAAAACCGTAAAGCGGGATTACCCGGAGGTTCCGGTGGTCATC
ATCTCGGGGCACGGGAATATCGAAATTGCGGTCGCGGCGATCAAGCAGGGCGCTTATGACTTCATCGAGAAGCCCTTTAA
TATCGACCAGTTGATGGTTGTGATCCGGCGCGCGATGGAGACCTCTCGCCTGCGTCGCGAAAACACGGATCTGCGCCGCA
AGGATGTAGGTGCAACCGATATGGTCGGGACGTCGCCCGCGTTTCGTGCCTTGACCAGCCAGCTTGACAAGGTGACGAAA
TCCAATGGGCGCGTCATGCTCACCGGGCCTGCGGGCAGCGGCAAGGAAGTAGCAGCCCGCTATATTCACGCAAACTCCCA
CCGCGCCAGTGAGCCTTTTGTCACCGTGAATTGCGCCAGCATTGAGCCCGAGCGCATGGAAGATGTGCTCTTTGGGCGTG
AGAGCAGCGAACGCGGCGTCGAGCCGGGGCTGCTGGAGCAGGCAGATGGGGGCGTCGTCTTTTTTGACGAAGTGGCGGAT
ATGCCCCCCGGCACCCAATCCAAGATCCTGCGCGTGCTGGTGGATCAGCAGTTTGTGCGCGTTGGCGGCACCGAGAAGGT
CAAGGTGGACCTACGGGTGATCTCGGCCACAAATCGCGATCTCGAACAGGAGATCAAGGATGGCAACTTCCGTCAGGAAT
TGTATCACCGTCTGAATGTGGTTCCGATCTGCGTGCCATCGCTCTCGGACCGGCGCGAGGACATCCCGCTGCTGGCGGAG
TATTTCATCCAAGGCTTCAACCAGACGCAGGGCCTGCCACTGCGCGCCCTTAGCGAAGACGCCGTGGCCTTGATGCAGAC
GATGACCTGGCCGGGCAATGTGCGCCAGCTGAAGAATCTGGTGGAACGCGTTCTGATCCTTGGAGAAACCTCCGGTCCCA
TTCAGGCAAAGGATCTGCCGAGCGAAGAAGAAAAGGTCGAAACAGAAGGTCGCGTCGTGCTCTCTGGCGCCTTGGCTACG
CTGCCTTTGCGCGAGGCACGCGAGGCCTTTGAACGCGAATATCTTCTGACGCAGATCAATCGCTTTGGCGGCAACATCAG
CCGCACGGCCTCCTTTGTGGGCATGGAACGCTCGGCGCTCCACCGCAAACTGAAATCGCTCGGCGTTGTCACATCGAACA
AGGCGGGCGCGCGGGTGGCGCGGGTCGAGAGCGAGGAAGCCACGGAAGAGACAGCGTAA

Upstream 100 bases:

>100_bases
TTGACGGGATGAGCCACCAAGGCGCAATGGCGGTGATCCGTCTCCCGGCGCTGGCGCCAGGAAATGCCGTCCAGAAACAA
ACTATAGTAGCAGGGCTGAC

Downstream 100 bases:

>100_bases
ATCAGGTGTAGGCCAGGGCTGCGGCGGGACAATTGAATTGACCCCCTTCGCACCATCTGGCATGCAAATCAGGCAGTCCA
GCGCCCGGAGAGCGGATCTA

Product: two component, sigma54 specific, Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSDILIVDDERDIRELVSDILEDESYATRKAANSEECMAAFEDAPPALLILDIWLKDSQMDGIDILKTVKRDYPEVPVVI
ISGHGNIEIAVAAIKQGAYDFIEKPFNIDQLMVVIRRAMETSRLRRENTDLRRKDVGATDMVGTSPAFRALTSQLDKVTK
SNGRVMLTGPAGSGKEVAARYIHANSHRASEPFVTVNCASIEPERMEDVLFGRESSERGVEPGLLEQADGGVVFFDEVAD
MPPGTQSKILRVLVDQQFVRVGGTEKVKVDLRVISATNRDLEQEIKDGNFRQELYHRLNVVPICVPSLSDRREDIPLLAE
YFIQGFNQTQGLPLRALSEDAVALMQTMTWPGNVRQLKNLVERVLILGETSGPIQAKDLPSEEEKVETEGRVVLSGALAT
LPLREAREAFEREYLLTQINRFGGNISRTASFVGMERSALHRKLKSLGVVTSNKAGARVARVESEEATEETA

Sequences:

>Translated_472_residues
MSDILIVDDERDIRELVSDILEDESYATRKAANSEECMAAFEDAPPALLILDIWLKDSQMDGIDILKTVKRDYPEVPVVI
ISGHGNIEIAVAAIKQGAYDFIEKPFNIDQLMVVIRRAMETSRLRRENTDLRRKDVGATDMVGTSPAFRALTSQLDKVTK
SNGRVMLTGPAGSGKEVAARYIHANSHRASEPFVTVNCASIEPERMEDVLFGRESSERGVEPGLLEQADGGVVFFDEVAD
MPPGTQSKILRVLVDQQFVRVGGTEKVKVDLRVISATNRDLEQEIKDGNFRQELYHRLNVVPICVPSLSDRREDIPLLAE
YFIQGFNQTQGLPLRALSEDAVALMQTMTWPGNVRQLKNLVERVLILGETSGPIQAKDLPSEEEKVETEGRVVLSGALAT
LPLREAREAFEREYLLTQINRFGGNISRTASFVGMERSALHRKLKSLGVVTSNKAGARVARVESEEATEETA
>Mature_471_residues
SDILIVDDERDIRELVSDILEDESYATRKAANSEECMAAFEDAPPALLILDIWLKDSQMDGIDILKTVKRDYPEVPVVII
SGHGNIEIAVAAIKQGAYDFIEKPFNIDQLMVVIRRAMETSRLRRENTDLRRKDVGATDMVGTSPAFRALTSQLDKVTKS
NGRVMLTGPAGSGKEVAARYIHANSHRASEPFVTVNCASIEPERMEDVLFGRESSERGVEPGLLEQADGGVVFFDEVADM
PPGTQSKILRVLVDQQFVRVGGTEKVKVDLRVISATNRDLEQEIKDGNFRQELYHRLNVVPICVPSLSDRREDIPLLAEY
FIQGFNQTQGLPLRALSEDAVALMQTMTWPGNVRQLKNLVERVLILGETSGPIQAKDLPSEEEKVETEGRVVLSGALATL
PLREAREAFEREYLLTQINRFGGNISRTASFVGMERSALHRKLKSLGVVTSNKAGARVARVESEEATEETA

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=454, Percent_Identity=34.5814977973568, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI1790299, Length=467, Percent_Identity=34.475374732334, Blast_Score=291, Evalue=4e-80,
Organism=Escherichia coli, GI1790437, Length=452, Percent_Identity=35.3982300884956, Blast_Score=270, Evalue=2e-73,
Organism=Escherichia coli, GI1788905, Length=452, Percent_Identity=35.3982300884956, Blast_Score=248, Evalue=6e-67,
Organism=Escherichia coli, GI87082117, Length=344, Percent_Identity=35.1744186046512, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1787583, Length=340, Percent_Identity=35.5882352941176, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI87082152, Length=323, Percent_Identity=35.6037151702786, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1789233, Length=321, Percent_Identity=33.6448598130841, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1789087, Length=323, Percent_Identity=34.984520123839, Blast_Score=181, Evalue=9e-47,
Organism=Escherichia coli, GI1786524, Length=328, Percent_Identity=34.1463414634146, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI87081872, Length=315, Percent_Identity=33.015873015873, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI1789828, Length=248, Percent_Identity=33.4677419354839, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI87081858, Length=306, Percent_Identity=27.4509803921569, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1786911, Length=119, Percent_Identity=31.9327731092437, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1786599, Length=118, Percent_Identity=32.2033898305085, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52364; Mature: 52233

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDILIVDDERDIRELVSDILEDESYATRKAANSEECMAAFEDAPPALLILDIWLKDSQM
CCCEEEECCCHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHCCCCEEEEEEEEECCCCC
DGIDILKTVKRDYPEVPVVIISGHGNIEIAVAAIKQGAYDFIEKPFNIDQLMVVIRRAME
CCHHHHHHHHHCCCCCCEEEEECCCCEEEEEEEHHHCCHHHHHCCCCHHHHHHHHHHHHH
TSRLRRENTDLRRKDVGATDMVGTSPAFRALTSQLDKVTKSNGRVMLTGPAGSGKEVAAR
HHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHH
YIHANSHRASEPFVTVNCASIEPERMEDVLFGRESSERGVEPGLLEQADGGVVFFDEVAD
HHCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEHHHCC
MPPGTQSKILRVLVDQQFVRVGGTEKVKVDLRVISATNRDLEQEIKDGNFRQELYHRLNV
CCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHHHHCCCHHHHHHHHCCE
VPICVPSLSDRREDIPLLAEYFIQGFNQTQGLPLRALSEDAVALMQTMTWPGNVRQLKNL
EEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
VERVLILGETSGPIQAKDLPSEEEKVETEGRVVLSGALATLPLREAREAFEREYLLTQIN
HHHHHEECCCCCCCCCCCCCCHHHHHCCCCCEEEECHHHHCCHHHHHHHHHHHHHHHHHH
RFGGNISRTASFVGMERSALHRKLKSLGVVTSNKAGARVARVESEEATEETA
HHCCCHHHHHHHHCCHHHHHHHHHHHCCCEECCCCCCEEEECCCHHHHCCCC
>Mature Secondary Structure 
SDILIVDDERDIRELVSDILEDESYATRKAANSEECMAAFEDAPPALLILDIWLKDSQM
CCEEEECCCHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHCCCCEEEEEEEEECCCCC
DGIDILKTVKRDYPEVPVVIISGHGNIEIAVAAIKQGAYDFIEKPFNIDQLMVVIRRAME
CCHHHHHHHHHCCCCCCEEEEECCCCEEEEEEEHHHCCHHHHHCCCCHHHHHHHHHHHHH
TSRLRRENTDLRRKDVGATDMVGTSPAFRALTSQLDKVTKSNGRVMLTGPAGSGKEVAAR
HHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHH
YIHANSHRASEPFVTVNCASIEPERMEDVLFGRESSERGVEPGLLEQADGGVVFFDEVAD
HHCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEHHHCC
MPPGTQSKILRVLVDQQFVRVGGTEKVKVDLRVISATNRDLEQEIKDGNFRQELYHRLNV
CCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHHHHCCCHHHHHHHHCCE
VPICVPSLSDRREDIPLLAEYFIQGFNQTQGLPLRALSEDAVALMQTMTWPGNVRQLKNL
EEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
VERVLILGETSGPIQAKDLPSEEEKVETEGRVVLSGALATLPLREAREAFEREYLLTQIN
HHHHHEECCCCCCCCCCCCCCHHHHHCCCCCEEEECHHHHCCHHHHHHHHHHHHHHHHHH
RFGGNISRTASFVGMERSALHRKLKSLGVVTSNKAGARVARVESEEATEETA
HHCCCHHHHHHHHCCHHHHHHHHHHHCCCEECCCCCCEEEECCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]