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The map label for this gene is hemB [H]

Identifier: 99081192

GI number: 99081192

Start: 1441498

End: 1442535

Strand: Direct

Name: hemB [H]

Synonym: TM1040_1351

Alternate gene names: 99081192

Gene position: 1441498-1442535 (Clockwise)

Preceding gene: 99081189

Following gene: 99081193

Centisome position: 45.03

GC content: 58.57

Gene sequence:

>1038_bases
ATGCAACCCATGTGCACCGGAACAGAATACGAGACCGAGATGCCCCATATCAGCGCTTCCTTTCCTGCCTCCCGCCCCCG
TCGCCTGCGTGCCTCTGCCGCTTTGCGCGACCTCACGCGGGAGAACGAACTCTCGGTGAATGACCTGATCTGGCCGGTTT
TTGTACGCGATGGGGAGGGGATCGAGGAGCCGGTTCCCTCGATGCCAGGCGTGGTGCGGCGCTCTGTCGACAAGGTGGTT
GAGGCCGCCGTCGAAGCGCAGGCACTCGGAATTCCGGCGATCTGCCTCTTTCCCTACACGGATCCGTCTTTGAAAACAGA
GGATTGTGCCGAGGCTTGGAACCCGGAGAACCTCTGCAATCGGGCCATCCGTGGGATCAAGGCCGCGGCGCCCGATCTCG
CCGTGATGACCGATGTCGCGCTAGATCCCTATAATATCAACGGCCACGACGGCTTTGTGATTGATGGCGAAATTCGCAAC
GACGAAACCGTCGAGGCGCTGGTCAAGATGACCCTCGCACAGGCCGAGGCCGGGGCGGATATCATCGGCCCCTCTGACAT
GATGGACGGGCGCATCGGAGCCATGCGGTCTGCATTGGAAAGGAAGGGATTTCAGAATGTTACGATCCTGTCCTACTCTG
CAAAATACGCGTCTGGATTTTATGGACCGTTTCGTGATGCGGTCGGCGCCTCGGGGGCCCTGACCGGCGACAAGAAGACC
TATCAGATGGACCCCGCCAACACCAATGAAGCCCTTCGCATGATTGAACGCGATCTGCGCGAGGGGGCGGATATGGTGAT
GGTGAAACCCGGATTGCCCTATCTCGACATCTGCCACCGCGTGAAAGAGACCTTCCAGGTCCCGACCTTTGCCTACCAGG
TGTCAGGAGAATACGCGATGATCCAAGCGGCGGCTCTGAATGGTTGGATCGATGGGGAAAAAGTTATGCTAGAAAGCCTC
ATGGCCTTCCGTCGGGCTGGATGTGATGGTGTGCTTACCTATTTTGCGCCACAGGTCGCGAAACTGTTGAACGGCTAA

Upstream 100 bases:

>100_bases
CTGCGCGCTGCCTGCCCTGTATCCATCGCCACGCTCTGCGACACAAGTGCTGCTGCGTCAGAAATGCGCGGATCGGAGAC
TTGTGAAGCACCACTGTTCC

Downstream 100 bases:

>100_bases
ACCTCTTTGCAAGACCCCGGCAGGAACCTGCTCAATCGGGCAGGCTTCAAAGTGACAGCTTTAGGGATTATGCATATAGT
TGTGTCAAACAAAGCGCCAA

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MQPMCTGTEYETEMPHISASFPASRPRRLRASAALRDLTRENELSVNDLIWPVFVRDGEGIEEPVPSMPGVVRRSVDKVV
EAAVEAQALGIPAICLFPYTDPSLKTEDCAEAWNPENLCNRAIRGIKAAAPDLAVMTDVALDPYNINGHDGFVIDGEIRN
DETVEALVKMTLAQAEAGADIIGPSDMMDGRIGAMRSALERKGFQNVTILSYSAKYASGFYGPFRDAVGASGALTGDKKT
YQMDPANTNEALRMIERDLREGADMVMVKPGLPYLDICHRVKETFQVPTFAYQVSGEYAMIQAAALNGWIDGEKVMLESL
MAFRRAGCDGVLTYFAPQVAKLLNG

Sequences:

>Translated_345_residues
MQPMCTGTEYETEMPHISASFPASRPRRLRASAALRDLTRENELSVNDLIWPVFVRDGEGIEEPVPSMPGVVRRSVDKVV
EAAVEAQALGIPAICLFPYTDPSLKTEDCAEAWNPENLCNRAIRGIKAAAPDLAVMTDVALDPYNINGHDGFVIDGEIRN
DETVEALVKMTLAQAEAGADIIGPSDMMDGRIGAMRSALERKGFQNVTILSYSAKYASGFYGPFRDAVGASGALTGDKKT
YQMDPANTNEALRMIERDLREGADMVMVKPGLPYLDICHRVKETFQVPTFAYQVSGEYAMIQAAALNGWIDGEKVMLESL
MAFRRAGCDGVLTYFAPQVAKLLNG
>Mature_345_residues
MQPMCTGTEYETEMPHISASFPASRPRRLRASAALRDLTRENELSVNDLIWPVFVRDGEGIEEPVPSMPGVVRRSVDKVV
EAAVEAQALGIPAICLFPYTDPSLKTEDCAEAWNPENLCNRAIRGIKAAAPDLAVMTDVALDPYNINGHDGFVIDGEIRN
DETVEALVKMTLAQAEAGADIIGPSDMMDGRIGAMRSALERKGFQNVTILSYSAKYASGFYGPFRDAVGASGALTGDKKT
YQMDPANTNEALRMIERDLREGADMVMVKPGLPYLDICHRVKETFQVPTFAYQVSGEYAMIQAAALNGWIDGEKVMLESL
MAFRRAGCDGVLTYFAPQVAKLLNG

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=309, Percent_Identity=41.4239482200647, Blast_Score=231, Evalue=1e-60,
Organism=Escherichia coli, GI87081728, Length=324, Percent_Identity=43.8271604938272, Blast_Score=247, Evalue=7e-67,
Organism=Saccharomyces cerevisiae, GI6321398, Length=346, Percent_Identity=35.8381502890173, Blast_Score=187, Evalue=3e-48,
Organism=Drosophila melanogaster, GI21358291, Length=307, Percent_Identity=38.4364820846906, Blast_Score=229, Evalue=2e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 37639; Mature: 37639

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPMCTGTEYETEMPHISASFPASRPRRLRASAALRDLTRENELSVNDLIWPVFVRDGEG
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHCEEEEEEECCCC
IEEPVPSMPGVVRRSVDKVVEAAVEAQALGIPAICLFPYTDPSLKTEDCAEAWNPENLCN
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHCCCHHHHHH
RAIRGIKAAAPDLAVMTDVALDPYNINGHDGFVIDGEIRNDETVEALVKMTLAQAEAGAD
HHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCC
IIGPSDMMDGRIGAMRSALERKGFQNVTILSYSAKYASGFYGPFRDAVGASGALTGDKKT
CCCCHHHCCCHHHHHHHHHHHCCCCCEEEEEECCHHHCCCCCHHHHHCCCCCCCCCCCCE
YQMDPANTNEALRMIERDLREGADMVMVKPGLPYLDICHRVKETFQVPTFAYQVSGEYAM
EEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCEEE
IQAAALNGWIDGEKVMLESLMAFRRAGCDGVLTYFAPQVAKLLNG
EEEHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQPMCTGTEYETEMPHISASFPASRPRRLRASAALRDLTRENELSVNDLIWPVFVRDGEG
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHCEEEEEEECCCC
IEEPVPSMPGVVRRSVDKVVEAAVEAQALGIPAICLFPYTDPSLKTEDCAEAWNPENLCN
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHCCCHHHHHH
RAIRGIKAAAPDLAVMTDVALDPYNINGHDGFVIDGEIRNDETVEALVKMTLAQAEAGAD
HHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCC
IIGPSDMMDGRIGAMRSALERKGFQNVTILSYSAKYASGFYGPFRDAVGASGALTGDKKT
CCCCHHHCCCHHHHHHHHHHHCCCCCEEEEEECCHHHCCCCCHHHHHCCCCCCCCCCCCE
YQMDPANTNEALRMIERDLREGADMVMVKPGLPYLDICHRVKETFQVPTFAYQVSGEYAM
EEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCEEE
IQAAALNGWIDGEKVMLESLMAFRRAGCDGVLTYFAPQVAKLLNG
EEEHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7784513 [H]