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The map label for this gene is bcr [H]

Identifier: 99081189

GI number: 99081189

Start: 1436054

End: 1437277

Strand: Direct

Name: bcr [H]

Synonym: TM1040_1348

Alternate gene names: 99081189

Gene position: 1436054-1437277 (Clockwise)

Preceding gene: 99081188

Following gene: 99081192

Centisome position: 44.86

GC content: 57.19

Gene sequence:

>1224_bases
ATGATTGAAAAACGCAAAAAAGCCCTGCCACAGGGCGAATTCATTGCTCTGATCGCGATGATGTTTGCAACCATCGCGTT
CTCGATCGACGCGATGCTGCCCGCACTTCCGGAAATCGGCGCAAGTCTAAGCCCTGACGATATAAATCAGGCTCAACTTA
TTATTTCTTCCTTTGTTCTTGGCATGGGGGTTGGCACCTTTTTTACCGGCCCCCTGTCAGATGCCTTCGGGCGCAAGCCG
GTGATCTTTGTTGGCAGCGGTCTCTATTGCCTTGGCGCTCTGGCTTCGTTTTTCAGCCAGTCTCTGGAGACGATTTTGAT
CTCGCGGCTGCTGATGGGGCTTGGGGCTGCTGGCCCACGGGTGGTCGCGATCGCGATTGTGCGCGACCTTTATGTCGGGC
GCGACATGGCGCGCATCATATCGCTCGCGATGATGATTTTTACGCTGGTGCCCGCCTTTGCGCCCCTGCTCGGAAGCGGC
ATCATTGCTGTCTCCAGCTGGCGCGGAATCTTTGTCGCCTTTGCGGTGTTTTCGCTGATCACCGTGCTCTGGATGAGCAT
CCGTCTTGAGGAGCCCTTGCCCCGGGACAAGCGTCGGCCTTTCCGGTTGCCGCAGCTCATGGGCGCGCTTCGCGAGATGG
TCAGCCATCCAACCGTACGTCTCTCGATCATGGCGCAGACCTTTTGCATGTCGATGTTGTTCACTGTTATCATAATGGTA
CAGCCCGTTTATGATGTCGTGTTCGCACGCAGCGAGAGCTTTCCCTTCTGGTTCATGATGGTCGCTCTGATGGCCGGTAC
CGCCAGCTTGTTGAATGCCTCTTTGGTGGGGCGCATTGGTATGCGGCGCATGGTGACCTGGGCCCTGGGAGTTCAGATCC
TCATCAGCACAGGTGTCTTCGCCCTGACCCAGCTCTCACTTGGCGAGCCGACGAGCTTTTACATTTTTGTCTTCTGGCAG
ACGAGCGTATTCTTCATGGTAGGGCTCACCCTCGGAAATTTGAATGCCATCGCCATGGAGCCAATGGGGCATATCGCTGG
CATGGCAGCGTCCGTCATGGGCGCCCTTTCCACCGTGGCGGCGGCAGTGATTGCCGCGCCGATTGGCCTTCTGTTTGACG
GCACGATCATCCCGATGGTTGGCGGTGTTCTTGCAACCGCTGGGCTGGCGTTCATCGTAATGCTCTGGATGGGGCGCGTT
GAGGCGGCAGCAACTCCCGCATAA

Upstream 100 bases:

>100_bases
AGCACTTCCCGCCACATTCATATCGTGTTAGCGCCGTTGGGCTGGTGGCCGAACCTTTCGCAGAGTGCGCCTATGTTCAT
GTCACCGCGAGAGGTTACAC

Downstream 100 bases:

>100_bases
AAGAACAGTCCCTCTGTCAGGCAGAGGGACTGTTTTAAATCAAAACCTTATGTAGATCAGTTGATGGAGCGGCCATCGCC
CTAGCGGTTCTTCTTGCGCT

Product: major facilitator transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MIEKRKKALPQGEFIALIAMMFATIAFSIDAMLPALPEIGASLSPDDINQAQLIISSFVLGMGVGTFFTGPLSDAFGRKP
VIFVGSGLYCLGALASFFSQSLETILISRLLMGLGAAGPRVVAIAIVRDLYVGRDMARIISLAMMIFTLVPAFAPLLGSG
IIAVSSWRGIFVAFAVFSLITVLWMSIRLEEPLPRDKRRPFRLPQLMGALREMVSHPTVRLSIMAQTFCMSMLFTVIIMV
QPVYDVVFARSESFPFWFMMVALMAGTASLLNASLVGRIGMRRMVTWALGVQILISTGVFALTQLSLGEPTSFYIFVFWQ
TSVFFMVGLTLGNLNAIAMEPMGHIAGMAASVMGALSTVAAAVIAAPIGLLFDGTIIPMVGGVLATAGLAFIVMLWMGRV
EAAATPA

Sequences:

>Translated_407_residues
MIEKRKKALPQGEFIALIAMMFATIAFSIDAMLPALPEIGASLSPDDINQAQLIISSFVLGMGVGTFFTGPLSDAFGRKP
VIFVGSGLYCLGALASFFSQSLETILISRLLMGLGAAGPRVVAIAIVRDLYVGRDMARIISLAMMIFTLVPAFAPLLGSG
IIAVSSWRGIFVAFAVFSLITVLWMSIRLEEPLPRDKRRPFRLPQLMGALREMVSHPTVRLSIMAQTFCMSMLFTVIIMV
QPVYDVVFARSESFPFWFMMVALMAGTASLLNASLVGRIGMRRMVTWALGVQILISTGVFALTQLSLGEPTSFYIFVFWQ
TSVFFMVGLTLGNLNAIAMEPMGHIAGMAASVMGALSTVAAAVIAAPIGLLFDGTIIPMVGGVLATAGLAFIVMLWMGRV
EAAATPA
>Mature_407_residues
MIEKRKKALPQGEFIALIAMMFATIAFSIDAMLPALPEIGASLSPDDINQAQLIISSFVLGMGVGTFFTGPLSDAFGRKP
VIFVGSGLYCLGALASFFSQSLETILISRLLMGLGAAGPRVVAIAIVRDLYVGRDMARIISLAMMIFTLVPAFAPLLGSG
IIAVSSWRGIFVAFAVFSLITVLWMSIRLEEPLPRDKRRPFRLPQLMGALREMVSHPTVRLSIMAQTFCMSMLFTVIIMV
QPVYDVVFARSESFPFWFMMVALMAGTASLLNASLVGRIGMRRMVTWALGVQILISTGVFALTQLSLGEPTSFYIFVFWQ
TSVFFMVGLTLGNLNAIAMEPMGHIAGMAASVMGALSTVAAAVIAAPIGLLFDGTIIPMVGGVLATAGLAFIVMLWMGRV
EAAATPA

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=373, Percent_Identity=26.2734584450402, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI48994889, Length=191, Percent_Identity=31.413612565445, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790146, Length=177, Percent_Identity=33.8983050847458, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082312, Length=167, Percent_Identity=28.1437125748503, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43647; Mature: 43647

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
7.1 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
7.1 %Met     (Mature Protein)
7.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEKRKKALPQGEFIALIAMMFATIAFSIDAMLPALPEIGASLSPDDINQAQLIISSFVL
CCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
GMGVGTFFTGPLSDAFGRKPVIFVGSGLYCLGALASFFSQSLETILISRLLMGLGAAGPR
HHCHHHHCCCCCHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
VVAIAIVRDLYVGRDMARIISLAMMIFTLVPAFAPLLGSGIIAVSSWRGIFVAFAVFSLI
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHH
TVLWMSIRLEEPLPRDKRRPFRLPQLMGALREMVSHPTVRLSIMAQTFCMSMLFTVIIMV
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHH
QPVYDVVFARSESFPFWFMMVALMAGTASLLNASLVGRIGMRRMVTWALGVQILISTGVF
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALTQLSLGEPTSFYIFVFWQTSVFFMVGLTLGNLNAIAMEPMGHIAGMAASVMGALSTVA
HHHHHCCCCCCEEEEEEEEHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHH
AAVIAAPIGLLFDGTIIPMVGGVLATAGLAFIVMLWMGRVEAAATPA
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIEKRKKALPQGEFIALIAMMFATIAFSIDAMLPALPEIGASLSPDDINQAQLIISSFVL
CCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
GMGVGTFFTGPLSDAFGRKPVIFVGSGLYCLGALASFFSQSLETILISRLLMGLGAAGPR
HHCHHHHCCCCCHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
VVAIAIVRDLYVGRDMARIISLAMMIFTLVPAFAPLLGSGIIAVSSWRGIFVAFAVFSLI
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHH
TVLWMSIRLEEPLPRDKRRPFRLPQLMGALREMVSHPTVRLSIMAQTFCMSMLFTVIIMV
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHH
QPVYDVVFARSESFPFWFMMVALMAGTASLLNASLVGRIGMRRMVTWALGVQILISTGVF
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALTQLSLGEPTSFYIFVFWQTSVFFMVGLTLGNLNAIAMEPMGHIAGMAASVMGALSTVA
HHHHHCCCCCCEEEEEEEEHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHH
AAVIAAPIGLLFDGTIIPMVGGVLATAGLAFIVMLWMGRVEAAATPA
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]