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The map label for this gene is phoB [H]

Identifier: 99081098

GI number: 99081098

Start: 1354554

End: 1355243

Strand: Reverse

Name: phoB [H]

Synonym: TM1040_1257

Alternate gene names: 99081098

Gene position: 1355243-1354554 (Counterclockwise)

Preceding gene: 99081099

Following gene: 99081096

Centisome position: 42.34

GC content: 62.32

Gene sequence:

>690_bases
ATGGCAGTCGATCAGCCTACTGTTCTGGTCGTCGAGGACGAACTGGCGCAGCGCGAAGTGCTTGCTTACAATCTGGAGGC
GGACGGCTTTCGGGTGATCAAGGCGGAGAACGGTGAAGAGGCGCTGCTGCTCGTCGAAGAGGACATGCCTGACATCATCG
TACTCGACTGGATGATGCCCAATCTGACCGGAATCGAGATCTGCCGCCGCCTGAAGACGCGCACGGACACCCGGAGCATT
CCGATCATCATGCTCTCCGCGCGCTCGGAAGAGGTCGACAAGGTGCGCGGTCTGGAGACCGGCGCGGACGACTATGTGGT
GAAACCTTACTCCGTGATCGAGCTGATGGCCCGGGTGCGCACGCAACTGCGCCGCGTCCGGCCAGCCACCGTTGGCATGC
GGCTCGAGTTTGACGACATTATCCTTGACGCGGAAACGCACAAGGTGAGCCGCTCGGAACATCCGCTCAAGCTTGGCCCG
ACCGAGTTTCGCCTGCTCTCGACCTTTATGGAGAAGCCGGGCCGTGTCTGGAGCCGTGAACAGCTGCTGGATCGCGTCTG
GGGGCGGGATATCTATGTCGACACCCGCACCGTCGATGTTCACATCGGCCGTCTGCGCAAGGCACTGACCCAGCATGGGG
GCGATGATCCCGTCCGTACGGTGCGCGGTGCGGGCTATGCGCTCGGGTAG

Upstream 100 bases:

>100_bases
GATCGCAGAGCAGGTGGTCTATCTCGTGACCGGCAACAAGCCCGAGGAAGAACGCACAAAAGCCGACAATACGTCGGTGA
CCATGCAGGAGATCTAAGCC

Downstream 100 bases:

>100_bases
CCGACCACATTAGATTTGTACTGTAACGATTTATTGCGGCTCCGGTTTTCCGGAGCCGTTTTTCCATCAAACAGTGGGCT
TAGCGCGGCAAGGGATCTTC

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MAVDQPTVLVVEDELAQREVLAYNLEADGFRVIKAENGEEALLLVEEDMPDIIVLDWMMPNLTGIEICRRLKTRTDTRSI
PIIMLSARSEEVDKVRGLETGADDYVVKPYSVIELMARVRTQLRRVRPATVGMRLEFDDIILDAETHKVSRSEHPLKLGP
TEFRLLSTFMEKPGRVWSREQLLDRVWGRDIYVDTRTVDVHIGRLRKALTQHGGDDPVRTVRGAGYALG

Sequences:

>Translated_229_residues
MAVDQPTVLVVEDELAQREVLAYNLEADGFRVIKAENGEEALLLVEEDMPDIIVLDWMMPNLTGIEICRRLKTRTDTRSI
PIIMLSARSEEVDKVRGLETGADDYVVKPYSVIELMARVRTQLRRVRPATVGMRLEFDDIILDAETHKVSRSEHPLKLGP
TEFRLLSTFMEKPGRVWSREQLLDRVWGRDIYVDTRTVDVHIGRLRKALTQHGGDDPVRTVRGAGYALG
>Mature_228_residues
AVDQPTVLVVEDELAQREVLAYNLEADGFRVIKAENGEEALLLVEEDMPDIIVLDWMMPNLTGIEICRRLKTRTDTRSIP
IIMLSARSEEVDKVRGLETGADDYVVKPYSVIELMARVRTQLRRVRPATVGMRLEFDDIILDAETHKVSRSEHPLKLGPT
EFRLLSTFMEKPGRVWSREQLLDRVWGRDIYVDTRTVDVHIGRLRKALTQHGGDDPVRTVRGAGYALG

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=219, Percent_Identity=52.0547945205479, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI1790860, Length=226, Percent_Identity=43.3628318584071, Blast_Score=163, Evalue=8e-42,
Organism=Escherichia coli, GI1786911, Length=226, Percent_Identity=40.2654867256637, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1788394, Length=225, Percent_Identity=38.6666666666667, Blast_Score=150, Evalue=8e-38,
Organism=Escherichia coli, GI1786784, Length=221, Percent_Identity=37.5565610859729, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI87082012, Length=221, Percent_Identity=35.2941176470588, Blast_Score=133, Evalue=9e-33,
Organism=Escherichia coli, GI1789809, Length=232, Percent_Identity=34.9137931034483, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1790863, Length=231, Percent_Identity=34.1991341991342, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1787375, Length=219, Percent_Identity=32.8767123287671, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI2367329, Length=229, Percent_Identity=37.5545851528384, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1789402, Length=225, Percent_Identity=32.8888888888889, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1787229, Length=229, Percent_Identity=33.1877729257642, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1790552, Length=224, Percent_Identity=33.4821428571429, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI145693140, Length=232, Percent_Identity=27.5862068965517, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1788550, Length=108, Percent_Identity=35.1851851851852, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1790299, Length=141, Percent_Identity=29.7872340425532, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1788191, Length=116, Percent_Identity=28.448275862069, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1790437, Length=153, Percent_Identity=28.7581699346405, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26079; Mature: 25948

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVDQPTVLVVEDELAQREVLAYNLEADGFRVIKAENGEEALLLVEEDMPDIIVLDWMMP
CCCCCCEEEEEECCHHCCCCEEEEECCCCEEEEEECCCCEEEEEEECCCCCEEEEECCCC
NLTGIEICRRLKTRTDTRSIPIIMLSARSEEVDKVRGLETGADDYVVKPYSVIELMARVR
CCCHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHCCCCCCCCEEECCHHHHHHHHHHH
TQLRRVRPATVGMRLEFDDIILDAETHKVSRSEHPLKLGPTEFRLLSTFMEKPGRVWSRE
HHHHHCCCHHEEEEEEHHHEEEECCHHHCCCCCCCEEECCHHHHHHHHHHHCCCCCCCHH
QLLDRVWGRDIYVDTRTVDVHIGRLRKALTQHGGDDPVRTVRGAGYALG
HHHHHHCCCEEEEEEEEEEEHHHHHHHHHHHCCCCCHHHHHCCCCCCCC
>Mature Secondary Structure 
AVDQPTVLVVEDELAQREVLAYNLEADGFRVIKAENGEEALLLVEEDMPDIIVLDWMMP
CCCCCEEEEEECCHHCCCCEEEEECCCCEEEEEECCCCEEEEEEECCCCCEEEEECCCC
NLTGIEICRRLKTRTDTRSIPIIMLSARSEEVDKVRGLETGADDYVVKPYSVIELMARVR
CCCHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHCCCCCCCCEEECCHHHHHHHHHHH
TQLRRVRPATVGMRLEFDDIILDAETHKVSRSEHPLKLGPTEFRLLSTFMEKPGRVWSRE
HHHHHCCCHHEEEEEEHHHEEEECCHHHCCCCCCCEEECCHHHHHHHHHHHCCCCCCCHH
QLLDRVWGRDIYVDTRTVDVHIGRLRKALTQHGGDDPVRTVRGAGYALG
HHHHHHCCCEEEEEEEEEEEHHHHHHHHHHHCCCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]