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The map label for this gene is nuoN [H]

Identifier: 99080601

GI number: 99080601

Start: 815747

End: 817183

Strand: Direct

Name: nuoN [H]

Synonym: TM1040_0760

Alternate gene names: 99080601

Gene position: 815747-817183 (Clockwise)

Preceding gene: 99080600

Following gene: 99080602

Centisome position: 25.48

GC content: 59.85

Gene sequence:

>1437_bases
ATGCAAGCTGATCTCTCCGTGATCCTGCCCGAGATCATCCTCGCCGTCTTCTCGATGGCGGCGCTGCTCGGAGCTGTCTA
CACCGTCAAGGACAAGCTGGCGCCGATGCTCGTGTGGGTAACCGCCGCGCTGATGGTGGTGCTGGGTATCTGGATCGGAA
CCTCCGGTTCTGGCGCTACCGAGGCGTTCGACGGCATGATCGTCAACGATGCCTTTGCGCGCTTTGCCAAGGTCGCGATC
CTCTTTTCTGCCGCCGCTGTCATGGTGATGGGGCAGGACTACATGTCCAAACGCGGCATGCTGCGGTTCGAATATCCGAT
CCTGATCGCGCTTTCTGTCATTGGTATGATGGTGATGGTCTCTGCCGGCGATCTGATGGCGCTTTATATGGGGCTGGAGC
TGCAATCGCTGGCGCTTTATGTGGTGGCTGCGCTGCGTCGCGACAGCGTCAAATCCACCGAGGCGGGCCTCAAGTATTTC
GTGCTTGGCGCGCTGTCTTCTGGTCTGCTGCTCTATGGCGCGTCGCTGGTCTACGGTTTTGCTGGCACCACAAAATTTGC
CGGTATCATCGAGGTTGCCCAACATGGAGAAGTCTCCATTGGCCTCTTGTTCGGCATCGTCTTCCTGATCTCGGGCATGG
CGTTCAAAGTCTCTGCGGTTCCGTTTCACATGTGGACCCCGGATGTCTACGAGGGCGCGCCGACGCCGATCACAGCGTTC
TTTGCCTCCGCGCCCAAAGTCGCCGCCATGGGGCTCTTTGCCCGCGTGCTGCACGACGCCTTTGGCCATGCGATCGAGGA
CTGGAGCCAGGTTGTCGCACTCTTGTCGGTGCTGTCGATGTTCCTTGGCGCAGTTGCGGCGATTGGGCAGACCGACCTCA
AGCGTCTGATGGCCTATTCGTCGATTGCACATATGGGCTATGCGCTCATCGGTCTGGCCGCAGGCACCGCGCTGGGTGTG
CAGGCGCTACTCATCTACATGTCGATTTATGTGGCGATGAACATCGGTACCTTTGCGTTCATCATGATGCTGCACCGCGA
CGGCAAGCCGGTCACCGACATTCGCGCCTTGAACCAATACGCCAAACGCGAACCTGGTCGTGCGCTTGGGATGCTGGTGC
TGTTGTTCTCGCTCGCGGGCGTGCCGCCGATGCTTGGCTTCTTTGGCAAGTTCTTCGTGCTGCGCGCAGCAGTCGAGGCC
GATATGGCCTGGCTTGCAATTGCGGGTGTGATCGCCTCGGTCATTGGGGCCTACTACTACCTGCGGATCGTCTTCTTCAT
GTACTTCGGTGCCGAAGGGGACGATGTGGAGACTTCGCGCTCGCCTGTCTTGCTCGCCTTCTTCACGGTTGGGGCGGCTT
TGATGCTGGTCGGTGTTGTAACCACCTTTGGTGTCGAGGGGGCCGCGGCCTCGGCGGCGGCCATGCTGGTCAACTGA

Upstream 100 bases:

>100_bases
GGTGCAATCCGTCAACACGGCCGTTGCCGACGCGGGGCCCCTCTGGGATGCCGCCGAGGTCGATGTCGCCGCCACCAATT
CCCACTAAGGAGCTGATGAG

Downstream 100 bases:

>100_bases
TGCGCTGCATCACGTTTTTGCACAAAGGCCCGGTCCAACGCGACCGGGCCTTTTGCCTTTTGCGAAACTGGGTGCTAAAG
GGGCGCGCATGACAGATTGG

Product: NADH dehydrogenase subunit N

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MQADLSVILPEIILAVFSMAALLGAVYTVKDKLAPMLVWVTAALMVVLGIWIGTSGSGATEAFDGMIVNDAFARFAKVAI
LFSAAAVMVMGQDYMSKRGMLRFEYPILIALSVIGMMVMVSAGDLMALYMGLELQSLALYVVAALRRDSVKSTEAGLKYF
VLGALSSGLLLYGASLVYGFAGTTKFAGIIEVAQHGEVSIGLLFGIVFLISGMAFKVSAVPFHMWTPDVYEGAPTPITAF
FASAPKVAAMGLFARVLHDAFGHAIEDWSQVVALLSVLSMFLGAVAAIGQTDLKRLMAYSSIAHMGYALIGLAAGTALGV
QALLIYMSIYVAMNIGTFAFIMMLHRDGKPVTDIRALNQYAKREPGRALGMLVLLFSLAGVPPMLGFFGKFFVLRAAVEA
DMAWLAIAGVIASVIGAYYYLRIVFFMYFGAEGDDVETSRSPVLLAFFTVGAALMLVGVVTTFGVEGAAASAAAMLVN

Sequences:

>Translated_478_residues
MQADLSVILPEIILAVFSMAALLGAVYTVKDKLAPMLVWVTAALMVVLGIWIGTSGSGATEAFDGMIVNDAFARFAKVAI
LFSAAAVMVMGQDYMSKRGMLRFEYPILIALSVIGMMVMVSAGDLMALYMGLELQSLALYVVAALRRDSVKSTEAGLKYF
VLGALSSGLLLYGASLVYGFAGTTKFAGIIEVAQHGEVSIGLLFGIVFLISGMAFKVSAVPFHMWTPDVYEGAPTPITAF
FASAPKVAAMGLFARVLHDAFGHAIEDWSQVVALLSVLSMFLGAVAAIGQTDLKRLMAYSSIAHMGYALIGLAAGTALGV
QALLIYMSIYVAMNIGTFAFIMMLHRDGKPVTDIRALNQYAKREPGRALGMLVLLFSLAGVPPMLGFFGKFFVLRAAVEA
DMAWLAIAGVIASVIGAYYYLRIVFFMYFGAEGDDVETSRSPVLLAFFTVGAALMLVGVVTTFGVEGAAASAAAMLVN
>Mature_478_residues
MQADLSVILPEIILAVFSMAALLGAVYTVKDKLAPMLVWVTAALMVVLGIWIGTSGSGATEAFDGMIVNDAFARFAKVAI
LFSAAAVMVMGQDYMSKRGMLRFEYPILIALSVIGMMVMVSAGDLMALYMGLELQSLALYVVAALRRDSVKSTEAGLKYF
VLGALSSGLLLYGASLVYGFAGTTKFAGIIEVAQHGEVSIGLLFGIVFLISGMAFKVSAVPFHMWTPDVYEGAPTPITAF
FASAPKVAAMGLFARVLHDAFGHAIEDWSQVVALLSVLSMFLGAVAAIGQTDLKRLMAYSSIAHMGYALIGLAAGTALGV
QALLIYMSIYVAMNIGTFAFIMMLHRDGKPVTDIRALNQYAKREPGRALGMLVLLFSLAGVPPMLGFFGKFFVLRAAVEA
DMAWLAIAGVIASVIGAYYYLRIVFFMYFGAEGDDVETSRSPVLLAFFTVGAALMLVGVVTTFGVEGAAASAAAMLVN

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Homo sapiens, GI251831108, Length=305, Percent_Identity=23.6065573770492, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI145693160, Length=381, Percent_Identity=34.3832020997375, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI1788614, Length=333, Percent_Identity=26.7267267267267, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788613, Length=340, Percent_Identity=26.7647058823529, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788831, Length=308, Percent_Identity=24.3506493506494, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788829, Length=251, Percent_Identity=29.8804780876494, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788827, Length=306, Percent_Identity=24.5098039215686, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 50669; Mature: 50669

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
6.3 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
6.3 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQADLSVILPEIILAVFSMAALLGAVYTVKDKLAPMLVWVTAALMVVLGIWIGTSGSGAT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
EAFDGMIVNDAFARFAKVAILFSAAAVMVMGQDYMSKRGMLRFEYPILIALSVIGMMVMV
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCEEECHHHHHHHHHHHHHHHH
SAGDLMALYMGLELQSLALYVVAALRRDSVKSTEAGLKYFVLGALSSGLLLYGASLVYGF
CCCHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
AGTTKFAGIIEVAQHGEVSIGLLFGIVFLISGMAFKVSAVPFHMWTPDVYEGAPTPITAF
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECEEECCCCHHCCCCCCCHHHH
FASAPKVAAMGLFARVLHDAFGHAIEDWSQVVALLSVLSMFLGAVAAIGQTDLKRLMAYS
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
SIAHMGYALIGLAAGTALGVQALLIYMSIYVAMNIGTFAFIMMLHRDGKPVTDIRALNQY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AKREPGRALGMLVLLFSLAGVPPMLGFFGKFFVLRAAVEADMAWLAIAGVIASVIGAYYY
HHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRIVFFMYFGAEGDDVETSRSPVLLAFFTVGAALMLVGVVTTFGVEGAAASAAAMLVN
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MQADLSVILPEIILAVFSMAALLGAVYTVKDKLAPMLVWVTAALMVVLGIWIGTSGSGAT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
EAFDGMIVNDAFARFAKVAILFSAAAVMVMGQDYMSKRGMLRFEYPILIALSVIGMMVMV
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCEEECHHHHHHHHHHHHHHHH
SAGDLMALYMGLELQSLALYVVAALRRDSVKSTEAGLKYFVLGALSSGLLLYGASLVYGF
CCCHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
AGTTKFAGIIEVAQHGEVSIGLLFGIVFLISGMAFKVSAVPFHMWTPDVYEGAPTPITAF
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECEEECCCCHHCCCCCCCHHHH
FASAPKVAAMGLFARVLHDAFGHAIEDWSQVVALLSVLSMFLGAVAAIGQTDLKRLMAYS
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
SIAHMGYALIGLAAGTALGVQALLIYMSIYVAMNIGTFAFIMMLHRDGKPVTDIRALNQY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AKREPGRALGMLVLLFSLAGVPPMLGFFGKFFVLRAAVEADMAWLAIAGVIASVIGAYYY
HHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRIVFFMYFGAEGDDVETSRSPVLLAFFTVGAALMLVGVVTTFGVEGAAASAAAMLVN
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA