Click here to switch to the map view.

The map label for this gene is nqo13 [H]

Identifier: 99080600

GI number: 99080600

Start: 814169

End: 815734

Strand: Direct

Name: nqo13 [H]

Synonym: TM1040_0759

Alternate gene names: 99080600

Gene position: 814169-815734 (Clockwise)

Preceding gene: 99080599

Following gene: 99080601

Centisome position: 25.44

GC content: 57.85

Gene sequence:

>1566_bases
ATGGACAATATTCTCTCCATCGTAACCTTCATTCCCGCACTGGCGGCCGCCGTGATGGCGCTGTTGATGCGCGGAGACGA
CGAGGCCGCACAGCGCAACGCGAAATATGTTGCGCTGATGGCCACCTCGATCACCTTTATCGTCTCGCTGTTCATCTATT
TTCAGTTCGATCCCTCCGACACGGGCTTTCAGTTCGTTGAGGAACGGGACTGGATCTTCGGGCTGAAGTACAAGATGGGC
GTCGACGGCATCTCTGTTCTGTTCGTGATGCTGACGACATTTGTGATGCCGCTGACGATCCTCGCCAGCTGGTCCGTGAC
CAACCGCGTCAAGGAATACATGATCGCCTTCCTGGTGCTTGAGACGCTGATGCTCGGCGTGTTCATGGCGCTCGACATGG
TGCTCTTTTACCTGTTCTTCGAGGCTGGCCTCATCCCGATGTTCCTCATCATCGGCATCTGGGGCGGCAAGGACCGCATC
TACGCGTCGTTCAAATTCTTCCTCTACACCTTCTTTGGCTCGGTGCTGATGCTGGTGGCGATGGTGGCAATGTATTTTGA
TGCGGGCACCACCGACATTGTGAAACTGTTGTCGCACGAGTTCTCCTATGAGGGTTTCAATGTGCTCGGGATTCACGTGC
TGGGCGGCATGCAGACGCTGCTGTTCCTCGCCTTCTTTGCCTCTTTTGCGGTGAAGATGCCGATGTGGCCGGTTCACACC
TGGTTGCCGGATGCGCACGTTCAGGCGCCGACGGCGGGCTCCGTGGTGCTGGCGGCAATCCTGCTGAAGATGGGGGGCTA
CGGCTTCCTGCGTTTCAGCCTGCCGATGTTCCCCGTGGGCTCAGAGGTGATGACCGATGTGATCCTGTGGATGTCGGCGA
TTGCGATTGTCTACACTTCGCTTGTGGCGATGGTGCAGGAAGACATGAAAAAGCTGATCGCGTATTCCTCGGTCGCCCAT
ATGGGCTTTGTGACCATGGGGATCTTTGCGGCGAACCAGCAGGGTGTGGATGGTGCGATTTTCCAGATGCTCAGCCACGG
CTTCATCTCGGCGGCGCTCTTCCTATGCGTGGGCGTGATCTATGATCGCATGCACACCCGCGACATCGACGCTTACGGTG
GCCTCGTGATCCGCATGCCGGCCTATGCGCTTGTGTTCATGTTCTTCACCATGGGCAACGTCGGTCTGCCCGGCACCTCC
GGCTTTGTCGGCGAATTCCTGACCTTGATGGGCGCCTTCCAGGAGAACACCTGGGTTGCGTTTGTGGCCACCACCGGTGT
GATCTTTTCGGCGTGTTACGCGCTCTGGCTCTATCGTCGGGTGGTTTTTGGCGACCTGATCAAAGAGAGCCTGAAGGCGA
TCAAGGACATGAACGCCCGCGAGAAATGGATCTTTGCGCCGCTCATCGTCATGACGCTCCTTTTGGGTGTCTATCCTTCT
GTTGTCACTGACGTGATCGGTCCCTCGACCGAAGCGCTGGTGCAATCCGTCAACACGGCCGTTGCCGACGCGGGGCCCCT
CTGGGATGCCGCCGAGGTCGATGTCGCCGCCACCAATTCCCACTAA

Upstream 100 bases:

>100_bases
GCCGGGCCCAGTCCGGTTACATCTTCACTTATGCCTTCTGGATGGTGCTCGGGATCGCAGCCCTTGTCACCTGGATGTCG
ATCGGCGGAGGATCGCACTG

Downstream 100 bases:

>100_bases
GGAGCTGATGAGATGCAAGCTGATCTCTCCGTGATCCTGCCCGAGATCATCCTCGCCGTCTTCTCGATGGCGGCGCTGCT
CGGAGCTGTCTACACCGTCA

Product: NADH dehydrogenase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I, chain 13; NDH-1, chain 13 [H]

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MDNILSIVTFIPALAAAVMALLMRGDDEAAQRNAKYVALMATSITFIVSLFIYFQFDPSDTGFQFVEERDWIFGLKYKMG
VDGISVLFVMLTTFVMPLTILASWSVTNRVKEYMIAFLVLETLMLGVFMALDMVLFYLFFEAGLIPMFLIIGIWGGKDRI
YASFKFFLYTFFGSVLMLVAMVAMYFDAGTTDIVKLLSHEFSYEGFNVLGIHVLGGMQTLLFLAFFASFAVKMPMWPVHT
WLPDAHVQAPTAGSVVLAAILLKMGGYGFLRFSLPMFPVGSEVMTDVILWMSAIAIVYTSLVAMVQEDMKKLIAYSSVAH
MGFVTMGIFAANQQGVDGAIFQMLSHGFISAALFLCVGVIYDRMHTRDIDAYGGLVIRMPAYALVFMFFTMGNVGLPGTS
GFVGEFLTLMGAFQENTWVAFVATTGVIFSACYALWLYRRVVFGDLIKESLKAIKDMNAREKWIFAPLIVMTLLLGVYPS
VVTDVIGPSTEALVQSVNTAVADAGPLWDAAEVDVAATNSH

Sequences:

>Translated_521_residues
MDNILSIVTFIPALAAAVMALLMRGDDEAAQRNAKYVALMATSITFIVSLFIYFQFDPSDTGFQFVEERDWIFGLKYKMG
VDGISVLFVMLTTFVMPLTILASWSVTNRVKEYMIAFLVLETLMLGVFMALDMVLFYLFFEAGLIPMFLIIGIWGGKDRI
YASFKFFLYTFFGSVLMLVAMVAMYFDAGTTDIVKLLSHEFSYEGFNVLGIHVLGGMQTLLFLAFFASFAVKMPMWPVHT
WLPDAHVQAPTAGSVVLAAILLKMGGYGFLRFSLPMFPVGSEVMTDVILWMSAIAIVYTSLVAMVQEDMKKLIAYSSVAH
MGFVTMGIFAANQQGVDGAIFQMLSHGFISAALFLCVGVIYDRMHTRDIDAYGGLVIRMPAYALVFMFFTMGNVGLPGTS
GFVGEFLTLMGAFQENTWVAFVATTGVIFSACYALWLYRRVVFGDLIKESLKAIKDMNAREKWIFAPLIVMTLLLGVYPS
VVTDVIGPSTEALVQSVNTAVADAGPLWDAAEVDVAATNSH
>Mature_521_residues
MDNILSIVTFIPALAAAVMALLMRGDDEAAQRNAKYVALMATSITFIVSLFIYFQFDPSDTGFQFVEERDWIFGLKYKMG
VDGISVLFVMLTTFVMPLTILASWSVTNRVKEYMIAFLVLETLMLGVFMALDMVLFYLFFEAGLIPMFLIIGIWGGKDRI
YASFKFFLYTFFGSVLMLVAMVAMYFDAGTTDIVKLLSHEFSYEGFNVLGIHVLGGMQTLLFLAFFASFAVKMPMWPVHT
WLPDAHVQAPTAGSVVLAAILLKMGGYGFLRFSLPMFPVGSEVMTDVILWMSAIAIVYTSLVAMVQEDMKKLIAYSSVAH
MGFVTMGIFAANQQGVDGAIFQMLSHGFISAALFLCVGVIYDRMHTRDIDAYGGLVIRMPAYALVFMFFTMGNVGLPGTS
GFVGEFLTLMGAFQENTWVAFVATTGVIFSACYALWLYRRVVFGDLIKESLKAIKDMNAREKWIFAPLIVMTLLLGVYPS
VVTDVIGPSTEALVQSVNTAVADAGPLWDAAEVDVAATNSH

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=379, Percent_Identity=32.1899736147757, Blast_Score=193, Evalue=3e-49,
Organism=Homo sapiens, GI251831117, Length=403, Percent_Identity=23.3250620347395, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1788613, Length=487, Percent_Identity=38.6036960985626, Blast_Score=308, Evalue=7e-85,
Organism=Escherichia coli, GI1788827, Length=276, Percent_Identity=27.8985507246377, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1788831, Length=297, Percent_Identity=26.9360269360269, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1788614, Length=430, Percent_Identity=26.046511627907, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI145693160, Length=434, Percent_Identity=25.8064516129032, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788829, Length=500, Percent_Identity=23.8, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI2367154, Length=134, Percent_Identity=29.1044776119403, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 57494; Mature: 57494

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
6.5 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
6.5 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNILSIVTFIPALAAAVMALLMRGDDEAAQRNAKYVALMATSITFIVSLFIYFQFDPSD
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHEEECCCC
TGFQFVEERDWIFGLKYKMGVDGISVLFVMLTTFVMPLTILASWSVTNRVKEYMIAFLVL
CCHHHHHCCCCEEEEEHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETLMLGVFMALDMVLFYLFFEAGLIPMFLIIGIWGGKDRIYASFKFFLYTFFGSVLMLVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
MVAMYFDAGTTDIVKLLSHEFSYEGFNVLGIHVLGGMQTLLFLAFFASFAVKMPMWPVHT
HHHHHHCCCHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
WLPDAHVQAPTAGSVVLAAILLKMGGYGFLRFSLPMFPVGSEVMTDVILWMSAIAIVYTS
CCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
LVAMVQEDMKKLIAYSSVAHMGFVTMGIFAANQQGVDGAIFQMLSHGFISAALFLCVGVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
YDRMHTRDIDAYGGLVIRMPAYALVFMFFTMGNVGLPGTSGFVGEFLTLMGAFQENTWVA
HHHHHHCCCHHHCCEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEE
FVATTGVIFSACYALWLYRRVVFGDLIKESLKAIKDMNAREKWIFAPLIVMTLLLGVYPS
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
VVTDVIGPSTEALVQSVNTAVADAGPLWDAAEVDVAATNSH
HHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCC
>Mature Secondary Structure
MDNILSIVTFIPALAAAVMALLMRGDDEAAQRNAKYVALMATSITFIVSLFIYFQFDPSD
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHEEECCCC
TGFQFVEERDWIFGLKYKMGVDGISVLFVMLTTFVMPLTILASWSVTNRVKEYMIAFLVL
CCHHHHHCCCCEEEEEHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETLMLGVFMALDMVLFYLFFEAGLIPMFLIIGIWGGKDRIYASFKFFLYTFFGSVLMLVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
MVAMYFDAGTTDIVKLLSHEFSYEGFNVLGIHVLGGMQTLLFLAFFASFAVKMPMWPVHT
HHHHHHCCCHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
WLPDAHVQAPTAGSVVLAAILLKMGGYGFLRFSLPMFPVGSEVMTDVILWMSAIAIVYTS
CCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
LVAMVQEDMKKLIAYSSVAHMGFVTMGIFAANQQGVDGAIFQMLSHGFISAALFLCVGVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
YDRMHTRDIDAYGGLVIRMPAYALVFMFFTMGNVGLPGTSGFVGEFLTLMGAFQENTWVA
HHHHHHCCCHHHCCEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEE
FVATTGVIFSACYALWLYRRVVFGDLIKESLKAIKDMNAREKWIFAPLIVMTLLLGVYPS
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
VVTDVIGPSTEALVQSVNTAVADAGPLWDAAEVDVAATNSH
HHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8422400 [H]