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The map label for this gene is birA [H]

Identifier: 99080602

GI number: 99080602

Start: 817272

End: 818021

Strand: Direct

Name: birA [H]

Synonym: TM1040_0761

Alternate gene names: 99080602

Gene position: 817272-818021 (Clockwise)

Preceding gene: 99080601

Following gene: 99080603

Centisome position: 25.53

GC content: 64.0

Gene sequence:

>750_bases
ATGACAGATTGGCCAATCGGATACGGGCGCCGGGTGCTGGCCGAAGTTGACAGCACCTTGGATGAAGCGGCCCGCCTCGC
GCCCGCACTGAGCGGGCCAGAGTGGATCCTTGCCCTGCGCCAGACCAAGGGGCGTGGGCGGCGGGGCCGTGCCTGGACCG
ACCCTAAGGGGAACTTTGCAGGCACGCTGGTCTATCGCCCGGAAGGCGCACCCGACCAGATCGCGCTACGCAGTTTTGTG
GCGGCTCTGGCGCTCTATGACGCCGTAGAGACCGTGACCGGCCGCACCACTGGGCTTGCGCTCAAATGGCCCAATGACGT
GCTGCTCAATGGCGGCAAGCTTGCGGGCATCCTCCTTGAGAGCTCAGGTGTGAAATCCGGTGTGGATCACCTCGCCATCG
GGATCGGGGTCAATCTCGCCGAGACGCCGATGAAGGAATGGCTGGAACCCGGTGCGGTCTGGCCCGTGTCGCTACAATCC
GAGACCGGCGTGCGCGTCGACCCGGAGACGTTTCTGTCGGCGCTTGCTCTTGCATTTGCCCGCTATGAGGACCAGTTCAC
CACATATGGGTTCGGACCGATCAGGGAGCTGTGGCTTTCGCGCGCGGCCAAGCTGGGCGAGGTCATCACCGCCCGCACCG
CGCGCGAGGAAACCTCGGGCACATTTGAAACCATCGACGACGCGGGCAACCTCGTTCTCAAGACCGCAAAGGGGATCGTC
ACCATCCCCGCAGCGGACATCTTTTTCTGA

Upstream 100 bases:

>100_bases
CTGGTCAACTGATGCGCTGCATCACGTTTTTGCACAAAGGCCCGGTCCAACGCGACCGGGCCTTTTGCCTTTTGCGAAAC
TGGGTGCTAAAGGGGCGCGC

Downstream 100 bases:

>100_bases
CGGAGGCGGGGTATATGCTTCTCGCGGTTGATTGCGGCAATACTAACACGGTTTTTGCGCTCTGGGATGGCGATCAGATG
ATCGGCCACTGGCGCACGTC

Product: biotin--acetyl-CoA-carboxylase ligase

Products: NA

Alternate protein names: Biotin--protein ligase [H]

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MTDWPIGYGRRVLAEVDSTLDEAARLAPALSGPEWILALRQTKGRGRRGRAWTDPKGNFAGTLVYRPEGAPDQIALRSFV
AALALYDAVETVTGRTTGLALKWPNDVLLNGGKLAGILLESSGVKSGVDHLAIGIGVNLAETPMKEWLEPGAVWPVSLQS
ETGVRVDPETFLSALALAFARYEDQFTTYGFGPIRELWLSRAAKLGEVITARTAREETSGTFETIDDAGNLVLKTAKGIV
TIPAADIFF

Sequences:

>Translated_249_residues
MTDWPIGYGRRVLAEVDSTLDEAARLAPALSGPEWILALRQTKGRGRRGRAWTDPKGNFAGTLVYRPEGAPDQIALRSFV
AALALYDAVETVTGRTTGLALKWPNDVLLNGGKLAGILLESSGVKSGVDHLAIGIGVNLAETPMKEWLEPGAVWPVSLQS
ETGVRVDPETFLSALALAFARYEDQFTTYGFGPIRELWLSRAAKLGEVITARTAREETSGTFETIDDAGNLVLKTAKGIV
TIPAADIFF
>Mature_248_residues
TDWPIGYGRRVLAEVDSTLDEAARLAPALSGPEWILALRQTKGRGRRGRAWTDPKGNFAGTLVYRPEGAPDQIALRSFVA
ALALYDAVETVTGRTTGLALKWPNDVLLNGGKLAGILLESSGVKSGVDHLAIGIGVNLAETPMKEWLEPGAVWPVSLQSE
TGVRVDPETFLSALALAFARYEDQFTTYGFGPIRELWLSRAAKLGEVITARTAREETSGTFETIDDAGNLVLKTAKGIVT
IPAADIFF

Specific function: Activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins [H]

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=228, Percent_Identity=29.8245614035088, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR003142
- InterPro:   IPR004143 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 26800; Mature: 26669

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDWPIGYGRRVLAEVDSTLDEAARLAPALSGPEWILALRQTKGRGRRGRAWTDPKGNFA
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEHHCCCCCCCCCCCCCCCCCEE
GTLVYRPEGAPDQIALRSFVAALALYDAVETVTGRTTGLALKWPNDVLLNGGKLAGILLE
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEECCCEEEEEEEE
SSGVKSGVDHLAIGIGVNLAETPMKEWLEPGAVWPVSLQSETGVRVDPETFLSALALAFA
CCCCCCCHHHEEEECCCCHHHHHHHHHCCCCCEEEEEECCCCCCEECHHHHHHHHHHHHH
RYEDQFTTYGFGPIRELWLSRAAKLGEVITARTAREETSGTFETIDDAGNLVLKTAKGIV
HHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEECCCEE
TIPAADIFF
EECCHHHCC
>Mature Secondary Structure 
TDWPIGYGRRVLAEVDSTLDEAARLAPALSGPEWILALRQTKGRGRRGRAWTDPKGNFA
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEHHCCCCCCCCCCCCCCCCCEE
GTLVYRPEGAPDQIALRSFVAALALYDAVETVTGRTTGLALKWPNDVLLNGGKLAGILLE
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEECCCEEEEEEEE
SSGVKSGVDHLAIGIGVNLAETPMKEWLEPGAVWPVSLQSETGVRVDPETFLSALALAFA
CCCCCCCHHHEEEECCCCHHHHHHHHHCCCCCEEEEEECCCCCCEECHHHHHHHHHHHHH
RYEDQFTTYGFGPIRELWLSRAAKLGEVITARTAREETSGTFETIDDAGNLVLKTAKGIV
HHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEECCCEE
TIPAADIFF
EECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8422400 [H]