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The map label for this gene is ynfJ [C]

Identifier: 99080534

GI number: 99080534

Start: 743914

End: 745608

Strand: Direct

Name: ynfJ [C]

Synonym: TM1040_0693

Alternate gene names: 99080534

Gene position: 743914-745608 (Clockwise)

Preceding gene: 99080533

Following gene: 99080537

Centisome position: 23.24

GC content: 61.0

Gene sequence:

>1695_bases
ATGGCGCGACGGGAAAGTAGTCCCCTGTCTGAATATCTCGCCAATACCAAGGCGCGCTTGCGGGATGCCCGTCGTGTATT
GGTTCACCGTGGACCAAGTCAGCTGCAATTCTGGTTGATTGCTCTGGTCATTGGGGTGCTCGCTGGCTTTGCCGCTTTGT
TCTTTCGCAAAGGCATTACCGCGCTACAAGGGGCCGTCTACGGCGCCGAGGATGTGGACTACCTGCATAGTTTCGCCGCA
ACGCTGCCGTGGTACGTGCTGTTGCTGGTGCCGGTATTCGGCGGGTTGATCGTCGGGATCATTCTGCACCGCTTTACGCC
AGACGGGCGGGTACGATCCGTCGCCGATGTGATCGAAGGCGCGGCCCTCAGAGATGGGCGCGTCGAGATCCGCGAGGGGT
TGGCCTCGGCCTGCGCGTCCTTTTTGACCCTCTCGACCGGGGGCTCTTCCGGGCGCGAGGGGCCGGTGGTCCATATCGCA
GGCATGATCTCTACCTGGGTGAGCGACCGCATCCGTGCCGATGGCATCACTGGGCGGGACCTCCTGGGTTGCGCGGTTGC
GGCAGCGGTTTCTGCCAGTTTTAACGCCCCGATTGCCGGCGCTCTTTTTGCCATGGAAGTGGTGCTCAGGCATTTTTCCG
TAAACACCTTCGCGCCCATCGTGGTGGCCTCGGTGGCAGGCACTGTCATCAATCGGCTTGAGTATGGGGATGTCACGGAA
TTTGTGCTCACGACGCCCGGTGCGTTGCAATTCTACGTCGAGTTGCCAGCGTTCCTGATGCTTGGTCTGATCTGTGGCGT
CGTCGCGGTTGTGCTGATGCATTCAATCTTTTTGGCGGATCGGGTGGGGACGGCTGTGCAATCGGCGCTTGGCATGCCGC
GCTGGTTGCGCCCGACCGTAGCTGGGTTTTTGCTTGGTCTGGTCGCAATCTGGTATCCGCATATCATTGGTGTTGGATAC
GAGACGACCGTGCTCGCCCTGTCTGGGAGCCTGCTGCTCAGCCAGGCTATCCTGTTCACGATTGTCAAAGTGGCTGCAGT
CTCCATCACCATGGGCGGGCGCATGGGGGGCGGGGTGTTTTCCCCCTCGTTGATGGTTGGGGCGCTGACCGGGCTTGCCT
TTGGCCTCATTGCGACGAATTTCCTGCCGGATGTCTCGGGTACGCATACGCTCTATGCTTTTGCGGGGATGGGCGCTGTC
GCCGCTGCGGTGCTGGGTGCGCCCATTTCGACCACGCTCATTGTGTTTGAACTGACAGGCGACTGGCAGATCGGCCTTGC
GGTGATGGTATCGGTGTCGATGTCCACGGCGTTGGCCTCGCGGATGGTGGACCGCTCGTTCTTCCTGACCCAGCTTGAAA
AACGCGACCTGCATTGCGCGGCGGGTCCGCAGGCCTACCTCTTGGGCACTCTGCGGGTGGGCAAGGTCATGCGGCCCTTG
GCGGATAGTGATCCCGAGAGCGCACAGGCGCTGATCGATGAAGGCATCGCCATCAGCCCCACCTCCACGCTGGAGGCGGC
GCTGCCGATTTTTGACCGGGCAGGGGTGGAGTATATCCCGGTGGTCACTGAAGAAGAGTTCTCAGAAGAGACCGGAGAGC
CACTGATGACCCTGATCGGCGTGCTCCATCATGTAGACTCGCTCAAGGCATACAACAGCGCGCTCGTGGCCATTGCGGCC
GAAGAACACCGGTAG

Upstream 100 bases:

>100_bases
TGGCAGACGTCTAAGGGTATCGGACGCTCGCAAAATTATTGTTAACTACCATGGGGTAGGTGTGGCACTGATAGGTCAGT
GAACAGATCGGAGAACCTGG

Downstream 100 bases:

>100_bases
TCTCGCATTTCGGATCTCGCACGGCTGCGCTGCCTGATGGGCGCTCCGTTTGTCCCGGGATTATTCGTGCCGCATTCCTG
ATGGGGGCGGGGGCGCAAAT

Product: Cl- channel, voltage gated

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 564; Mature: 563

Protein sequence:

>564_residues
MARRESSPLSEYLANTKARLRDARRVLVHRGPSQLQFWLIALVIGVLAGFAALFFRKGITALQGAVYGAEDVDYLHSFAA
TLPWYVLLLVPVFGGLIVGIILHRFTPDGRVRSVADVIEGAALRDGRVEIREGLASACASFLTLSTGGSSGREGPVVHIA
GMISTWVSDRIRADGITGRDLLGCAVAAAVSASFNAPIAGALFAMEVVLRHFSVNTFAPIVVASVAGTVINRLEYGDVTE
FVLTTPGALQFYVELPAFLMLGLICGVVAVVLMHSIFLADRVGTAVQSALGMPRWLRPTVAGFLLGLVAIWYPHIIGVGY
ETTVLALSGSLLLSQAILFTIVKVAAVSITMGGRMGGGVFSPSLMVGALTGLAFGLIATNFLPDVSGTHTLYAFAGMGAV
AAAVLGAPISTTLIVFELTGDWQIGLAVMVSVSMSTALASRMVDRSFFLTQLEKRDLHCAAGPQAYLLGTLRVGKVMRPL
ADSDPESAQALIDEGIAISPTSTLEAALPIFDRAGVEYIPVVTEEEFSEETGEPLMTLIGVLHHVDSLKAYNSALVAIAA
EEHR

Sequences:

>Translated_564_residues
MARRESSPLSEYLANTKARLRDARRVLVHRGPSQLQFWLIALVIGVLAGFAALFFRKGITALQGAVYGAEDVDYLHSFAA
TLPWYVLLLVPVFGGLIVGIILHRFTPDGRVRSVADVIEGAALRDGRVEIREGLASACASFLTLSTGGSSGREGPVVHIA
GMISTWVSDRIRADGITGRDLLGCAVAAAVSASFNAPIAGALFAMEVVLRHFSVNTFAPIVVASVAGTVINRLEYGDVTE
FVLTTPGALQFYVELPAFLMLGLICGVVAVVLMHSIFLADRVGTAVQSALGMPRWLRPTVAGFLLGLVAIWYPHIIGVGY
ETTVLALSGSLLLSQAILFTIVKVAAVSITMGGRMGGGVFSPSLMVGALTGLAFGLIATNFLPDVSGTHTLYAFAGMGAV
AAAVLGAPISTTLIVFELTGDWQIGLAVMVSVSMSTALASRMVDRSFFLTQLEKRDLHCAAGPQAYLLGTLRVGKVMRPL
ADSDPESAQALIDEGIAISPTSTLEAALPIFDRAGVEYIPVVTEEEFSEETGEPLMTLIGVLHHVDSLKAYNSALVAIAA
EEHR
>Mature_563_residues
ARRESSPLSEYLANTKARLRDARRVLVHRGPSQLQFWLIALVIGVLAGFAALFFRKGITALQGAVYGAEDVDYLHSFAAT
LPWYVLLLVPVFGGLIVGIILHRFTPDGRVRSVADVIEGAALRDGRVEIREGLASACASFLTLSTGGSSGREGPVVHIAG
MISTWVSDRIRADGITGRDLLGCAVAAAVSASFNAPIAGALFAMEVVLRHFSVNTFAPIVVASVAGTVINRLEYGDVTEF
VLTTPGALQFYVELPAFLMLGLICGVVAVVLMHSIFLADRVGTAVQSALGMPRWLRPTVAGFLLGLVAIWYPHIIGVGYE
TTVLALSGSLLLSQAILFTIVKVAAVSITMGGRMGGGVFSPSLMVGALTGLAFGLIATNFLPDVSGTHTLYAFAGMGAVA
AAVLGAPISTTLIVFELTGDWQIGLAVMVSVSMSTALASRMVDRSFFLTQLEKRDLHCAAGPQAYLLGTLRVGKVMRPLA
DSDPESAQALIDEGIAISPTSTLEAALPIFDRAGVEYIPVVTEEEFSEETGEPLMTLIGVLHHVDSLKAYNSALVAIAAE
EHR

Specific function: Unknown

COG id: COG0038

COG function: function code P; Chloride channel protein EriC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chloride channel (TC 2.A.49) family. ClcB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081943, Length=421, Percent_Identity=25.8907363420428, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786350, Length=429, Percent_Identity=27.039627039627, Blast_Score=97, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014743
- InterPro:   IPR001807 [H]

Pfam domain/function: PF00654 Voltage_CLC [H]

EC number: NA

Molecular weight: Translated: 59553; Mature: 59422

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRESSPLSEYLANTKARLRDARRVLVHRGPSQLQFWLIALVIGVLAGFAALFFRKGIT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALQGAVYGAEDVDYLHSFAATLPWYVLLLVPVFGGLIVGIILHRFTPDGRVRSVADVIEG
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
AALRDGRVEIREGLASACASFLTLSTGGSSGREGPVVHIAGMISTWVSDRIRADGITGRD
CCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCHH
LLGCAVAAAVSASFNAPIAGALFAMEVVLRHFSVNTFAPIVVASVAGTVINRLEYGDVTE
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHH
FVLTTPGALQFYVELPAFLMLGLICGVVAVVLMHSIFLADRVGTAVQSALGMPRWLRPTV
HHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
AGFLLGLVAIWYPHIIGVGYETTVLALSGSLLLSQAILFTIVKVAAVSITMGGRMGGGVF
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCC
SPSLMVGALTGLAFGLIATNFLPDVSGTHTLYAFAGMGAVAAAVLGAPISTTLIVFELTG
CHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECC
DWQIGLAVMVSVSMSTALASRMVDRSFFLTQLEKRDLHCAAGPQAYLLGTLRVGKVMRPL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
ADSDPESAQALIDEGIAISPTSTLEAALPIFDRAGVEYIPVVTEEEFSEETGEPLMTLIG
CCCCCHHHHHHHHCCCEECCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHCCHHHHHHHH
VLHHVDSLKAYNSALVAIAAEEHR
HHHHHHHHHHHCCEEEEEEECCCC
>Mature Secondary Structure 
ARRESSPLSEYLANTKARLRDARRVLVHRGPSQLQFWLIALVIGVLAGFAALFFRKGIT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALQGAVYGAEDVDYLHSFAATLPWYVLLLVPVFGGLIVGIILHRFTPDGRVRSVADVIEG
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
AALRDGRVEIREGLASACASFLTLSTGGSSGREGPVVHIAGMISTWVSDRIRADGITGRD
CCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCHH
LLGCAVAAAVSASFNAPIAGALFAMEVVLRHFSVNTFAPIVVASVAGTVINRLEYGDVTE
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHH
FVLTTPGALQFYVELPAFLMLGLICGVVAVVLMHSIFLADRVGTAVQSALGMPRWLRPTV
HHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
AGFLLGLVAIWYPHIIGVGYETTVLALSGSLLLSQAILFTIVKVAAVSITMGGRMGGGVF
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCC
SPSLMVGALTGLAFGLIATNFLPDVSGTHTLYAFAGMGAVAAAVLGAPISTTLIVFELTG
CHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECC
DWQIGLAVMVSVSMSTALASRMVDRSFFLTQLEKRDLHCAAGPQAYLLGTLRVGKVMRPL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
ADSDPESAQALIDEGIAISPTSTLEAALPIFDRAGVEYIPVVTEEEFSEETGEPLMTLIG
CCCCCHHHHHHHHCCCEECCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHCCHHHHHHHH
VLHHVDSLKAYNSALVAIAAEEHR
HHHHHHHHHHHCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]